FastQCFastQC Report
Sat 31 Dec 2022
SRR5844065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29833
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT3941.3206851473200818No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC3901.3072771762812991No Hit
TCTAAAGGGCAGCTACTATGGGTGATCTCAACATGATTTTTATTTGAAAGTG1520.5095028994737372No Hit
AGCTGGGACTCAGAGGGTGTCTCTGCAGTCTGGTGGATCTTGTCCATTGGGC1450.4860389501558676No Hit
TGGTGGGACCTAGAAATCCCTCTGGACACTTGTGGGTTCTTTAGGGTTTGAG1440.482686957396172No Hit
AGACACGGGGATTAGACAATGTGGAATTACATAACAGGCATTGCACTAAAAG1410.47263097911708507No Hit
GAAACGGGGAGTACAGGGAAACTTGCATATCGTCAAACAAATTTAGAAGCCC1380.4625750008379982No Hit
CGGCGGCGCGTCTGGAGGAGGCTGCAGCAGCGGAAGACCCCAGTCCAGATCC1320.4424630442798244No Hit
AGTTTTGGGAAGCCAACAACAGGGTCTTGAGATAACCTTTGAAGCTTATTGT1300.435759058760433No Hit
TTGAGCCGGGGCAGAGCTCGAGGAAGAACAGTCCCAGGGCTCCACCACAAAT1290.4324070660007374No Hit
TCGCCAGGGAGCCTTTAATTAGAATCCTAGTTTTCTCTCTAATCCAGTGGCA1250.4189990949619549No Hit
GTTACAGGGTCCTATAATGGGAGCATCACCACTTATTAAAACACACATAGAA1220.4089431166828679No Hit
CGATATGGGCACCTCAGGCCGCTGCTCAGATGCCTCTGCTGGAGCTGGCACT1220.4089431166828679No Hit
AATTGGGAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGT1220.4089431166828679No Hit
TTGATGGGGCAGCTGATGGACACTTGCGTTGCTTCCACCTTTGGGTTCTTGT1200.40223913116347665No Hit
CATGCGGGCAACGACACAAGAAACACAACTTCAGACTTGCAGAAAACCAGTG1170.3921831528843898No Hit
AGTTTGGGAGCACCTAGTGCGAGACAGTGTGTTCCAGCGCTGCGGCCTCCGC1150.3854791673649985No Hit
TTAGTGGGGGGGTGCGGGTGCCCGGGTGAGGGGCGGAGCTGGGGGCATGGCG1130.3787751818456072No Hit
TCAGTTGGGGGTTGGCTGAGGCGGGAGGATTACCTGTGTTTAGGAGTTTGAG1130.3787751818456072No Hit
TTATGGGGCACCCGCCATCCACCAGTCCTAACACCTCCCGACCAGGAAGTCA1110.37207119632621594No Hit
ATTTACGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1110.37207119632621594No Hit
CTTCATGGGGACGCTCTTCCGATTTGATACGGGGGCTCTTCCACAGGAGGCC1100.3687192035665203No Hit
TACGAAGGGATTTATTAATATGTAAAAGAGTGATAAACAAGGGAAAATAAAG1100.3687192035665203No Hit
AACACAGGGGCCCCATAGTTCAGCTTGTGAAACTTTCACGTCGTTGTGTTGG1100.3687192035665203No Hit
ACGAAGGGGCTGGCTGCCAATCCCATATACACACATTATAAGCAAAGATGCC1090.36536721080682466No Hit
TTGCTTGGGAAGAAACTGAAAGCTGTATGTCAACTTTATAAACATAATTCAA1060.3553112325277377No Hit
GCTACTGGGGAGGGGCGAGGCGGCTGGGGACCGCGGGGCGGACGGGAGCGAG1060.3553112325277377No Hit
CTCCAGGGGAGGCAATGATTACTTCGACACAGTGCCACATGGCTTTGAAACC1060.3553112325277377No Hit
TGAACCCGGGAGGCAGAGGTTGCAGTGGGCTGAGATCGCACCACTGCACTCC1040.34860724700834644No Hit
AGTCCAGGGTGTTGGAAATAAAATATAAGACATTGATCTAGATATGAGGTTT1030.34525525424865083No Hit
CAAACCGGGACCCACACCCAGACCTCTTGAGTAAGGACAGAAACGTTATCAT1030.34525525424865083No Hit
GATCACGGGTGTGGAAAAAAACAAGCTCACTTTGGGATTACCCACCCCGAAA1020.34190326148895517No Hit
CGCTATGGGGGGCGCACGCAGCCGCACTGGGGGTGCAAGACTACCTCCTGTT1010.33855126872925956No Hit
ACACAAGGGATGTTAAGTTTAAGATGGAGGTGCCAGGTAATTGGTTACAAGA1000.3351992759695639No Hit
CGTTAGGGGAAGATAGAACCTTTAGATACTCCAACTCACCAAAATGTAAAAA990.3318472832098683No Hit
GCCAATGGGATGCAATTTCCACTATCAATAATTTAACTTCTTTGCTGCAGAA980.3284952904501726No Hit
GGCTGTTAAAAGGGGAAAGTTATTCTGAGACACAATCCAAAAGAGAATGGTA980.3284952904501726No Hit
ATGTGCGGGCTTGAGAGGGGGTTCTAAGCAATGCAAATGTCTAATTGTCCCC960.32179130493078134No Hit
CCGTTAGGGATCTACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTC960.32179130493078134No Hit
TTACCGGGAACTCTGGAAATCACGTGTGTGGGGAGATGGGGACGCTTCCCAT950.31843931217108573No Hit
CGGGATGGGCAGATGCTTTTTCGTGTACATGATACTAGTAGACACTTTTCTC950.31843931217108573No Hit
TAGATAGGGGCTGGGATTACAGGCTTGAGCCACTCCGCCCTGCCTGTTAATT930.3117353266516944No Hit
GGTCACGGGATATAGGAATGTCCTCATGTTTCTCACTGAACTTTTGAAGTAG910.3050313411323031No Hit
CTGCACTAGGGCAGTGTATCAGCGACAAACAGTTTCAGCACCTCAGTGTCTC910.3050313411323031No Hit
ATTCATGGGAATTTTACAAATGGCTTTTTACAATTGGTTTCTCATAATCATG900.3016793483726075No Hit
CTTAAAGGGAACTCCCTGAATGACGACATCAGCCTCTACAAGAGCTTCCTCC890.29832735561291185No Hit
TGTCATGGGAAGTCAACCTAGAACTATACACAAATACAAACATGCCTAAAAT870.2916233700935206No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA870.2916233700935206No Hit
CACCCCGGCCCACTCCAGCGGCGACTTTGAGGGATTCCCTCTCTGGCGGCCT860.28827137733382496No Hit
GAACGAGGGATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGC850.28491938457412935No Hit
CTCTGCCGGGGGCGACGGCGGCGGCCGTCCTCATCCCGGCGCTTGAGAGGAC850.28491938457412935No Hit
GACGCGGAGCCTTGAAGACTGTGCTGGTGAGAAGAAGTCTGGGTGGGAGATG850.28491938457412935No Hit
CGTAGCGGGCAGAGAAACCAGCTTCCTTCAGAGAGCAGTGCTTTTGGCGGGG840.2815673918144337No Hit
AGAATGGGGTGTCATTTTTTATTAATTTACTGTGAAAACTATTACTGTTCAA830.2782153990547381No Hit
GGTATAGGTCTCAGGAATTTGTCAGAAAAAAGAATAAAATCACCTGAGACTC830.2782153990547381No Hit
AATCATGGGCGATCTTAGCTTGGGATGAGGTCTTTTTTTAAAATACCACTTT820.2748634062950424No Hit
GTAGGGGGGAACCGGATCGGTGACTGTGGAGGGCGAGCTGAGCCCTGGCCGC810.2715114135353468No Hit
CAGCCTGGGCAACAAGAGCGGGACTCCATCTCAAAAAAAATTAAAAAAAAGA800.2681594207756511No Hit
TGCAACGGGAGTTCCGCCATGGCCTCCTTGGAAGTCAGTCGTAGTCCTCGCA790.26480742801595547No Hit
AAGTGGGTGGTGGCTCCTCTGACAATATTAGGGGGTCCTCTTCTCATCAGAG790.26480742801595547No Hit
CAATCTGGGGGGCTCGTGGGGCGCCTGGAGTGAGGGTTCTGGTTCCCGCCGG790.26480742801595547No Hit
ATCCTAGGGGGGAAGCGTTGGAGGACATTCCCTGTTGACTGCGTCGCGATGT780.2614554352562598No Hit
ACTGCAGGGATGGCCGCTACTTCAGGAGCTGCCCCATCTCAGGCAGGGCCGT780.2614554352562598No Hit
AAACTTGGGGCCTGATGCTAAAGTTTTTAAAAATTACTTAAAGGTGTTCTTC770.2581034424965642No Hit
ATCCAAGGGGCTGGGGCCTAGCCTCGAGGACCTGTTCAACTTCTGTTCCCGC770.2581034424965642No Hit
TGTGCAGTGAACAAGACAAAGTCCCTGCTGTCATGGAACTTTCCATCTAGTG770.2581034424965642No Hit
TCGCATGGGACCAGGGGTCTAATCAGGCCTGTGTCTGCCTCCTTCTTGAATA720.241343478698086No Hit
ATCACCGGGAAGAAAACCTGAATCCTAATGCTCATCCAAGACCCACCTCCCA720.241343478698086No Hit
TATGCTGGGTCTCAAGAAGCACCTCGTGTCGGCCGGATATGTCCACGTGAAC710.23799148593839037No Hit
AGATTAGGGAATGCTTATTCTGAAAATCCTAGGAACCTTAAGAATGATGCTA680.22793550765930348No Hit
AGGCGTGGGGGGTGAAATACATCAAGCAGACCAGCGCCATCCTGCAGCAGCA680.22793550765930348No Hit
GAATCTGTTTCTATGTCCAAACCTGGTAAATTTTATAATGTCATATTGTTTG680.22793550765930348No Hit
TCTGCGGGGCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAACT680.22793550765930348No Hit
ATCAAGGGGATGAGGTCTTTTTTTTAAAATACCACTTTTAGTGTCAGTGAAC680.22793550765930348No Hit
GGCACTGGGAGAACCGGTGGAGCAGCGACCCCTGAGCAGTGTTCTCTGTGCT680.22793550765930348No Hit
TCAATGGGGTCACTCCCTGAAGTTCATTGCCACCCTTCAGTACATCGTGGGC680.22793550765930348No Hit
CTTACGCGGGGGAGGAAGGGGAGCTGAGGTCCGGGGCCCAGTTTTCCGGAGT680.22793550765930348No Hit
GCACATGGGGTGACGGGGGCTGTTGGGGGCCAGCAGGAGGATGAGGACAGCA670.22458351489960784No Hit
TCAATCGGGTGAATCTAACTTTGTTTTTGTTTTGTTTTTTTCCTTATGATTA660.2212315221399122No Hit
TTATTGGGGAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTAAGGCT660.2212315221399122No Hit
TCAGGGGGCTGGGGATCCTTGGATGGATTTCAGAGATTCTGTTGAATCCCCT660.2212315221399122No Hit
CGCCATGGGAACTTCTGACCTTGTGATCCACCCACCTTGGTCTCCCAAAGTG650.2178795293802165No Hit
ATACCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA640.21452753662052088No Hit
GACGATGGGGTTAAAAAGAAACATAGTGTTGCCCTTTTTCTTAAAGCATCAG630.21117554386082524No Hit
GGACTCGGGGGGAACGGGCGGCGGCGTTGGCGGCTTGTGCAGCAATGGCCAA620.2078235511011296No Hit
TTTGACGGGACTGGGGAAAAACCATACAAATGTGAAGAATGTGGCAAAGCCT620.2078235511011296No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTT610.20447155834143396No Hit
CAATAAGGGTATGCATATGATCTTTAATTATTGAAGAAAATAAAGTGAGGAC600.20111956558173832No Hit
GAATAAGGGTTGGTCACCAGTTGATATGTTGTCTTTGTAGAAAAGAATTCAA600.20111956558173832No Hit
AGTTATGGGCACCAATGACATGTTCAGGCTCGCCCTGCACAACTTTGGCTGA600.20111956558173832No Hit
GGCAGGGCACCATTTAATCATTTCCAGCAAAATATATGTTTGGCTGAAATTA580.19441558006234708No Hit
CTTCTAGGGCCAGTTTTGCCCTTCCTCAAGAATATATGGTTTTACAGCTGTG580.19441558006234708No Hit
AATAGGGGGAACTTTTCTAAGGAATGATACATAGAAAAGATTTTATTTTAAA570.19106358730265144No Hit
GTAGCCAGGCCATATAGGTATCCACAGAAAGGAGATCAGATTTCACTCTGAG570.19106358730265144No Hit
ACATACGGGGGCTGACTCTGGTTCAGTATTCTCTTAGGTGATTATATTCTCT570.19106358730265144No Hit
GCCTCAAGGGGTGAAGTAATTCCAGAAAGCACCAGTGCAGTAACAAACATTC560.1877115945429558No Hit
CGTGCAGGGAAATGGGAAATGTTATGACACAATCGAGGAGCTGAATAGAAGC560.1877115945429558No Hit
GCGCTGGGGTATGTTTCCTTCCTGGGAACAAGATGCTAGCGCGGTGCACCGG550.18435960178326016No Hit
AAGGCCGAGGCAGGTGGATCACAAGGTCAGGAGTTGGAGACCAGCCTGACCA550.18435960178326016No Hit
TTTAGGGGGACTATGCACCCTATCCAAATGGCTAGGTCACAACAGTGATTAA540.1810076090235645No Hit
GCCCATGGGTACAATGGCTCCAAAGAGATGCAATAAGCAACATTTAAGACTT540.1810076090235645No Hit
TCGACGGGGTAAATAAATCCTGCACCAAATCCCCAGGGGGAGAAATTAACAA540.1810076090235645No Hit
AGTTTGGGGTGACACTGGCTATGAACACTATGATGCTTACAACCTCTCTGCC540.1810076090235645No Hit
TCTTGAGGGGGAGGAGGAGTTGAGAGAACGGAGCGGACGCCATGGCGACCAA540.1810076090235645No Hit
GCGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA520.17430362350417322No Hit
GAGAAAGGGATGTAGTCAATATAACAAACAAGAGATAGCCTTTGAAATGGCA520.17430362350417322No Hit
GAGGCAGGAAAATCGCTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCTGAGA510.17095163074447758No Hit
GTTGTAGGGGCATGATCAAGCTAGGTCTAGGTATTGATGAAGATGAAGTGGC510.17095163074447758No Hit
TTATATGGGTGAATCCAAATGAGATAATTATACCACCCACTGAGGCACCTTA500.16759963798478195No Hit
ACTGCTGGGGGCAGACAATGATTGAAGCTCACGTTGATGTCAAGACTACCGA500.16759963798478195No Hit
GATCTTGAGGGGGGCGTCTCCAATATGACTATTAAAACTGTCACCTCTGGAA490.1642476452250863No Hit
TTCATAGGGAAGTGAGGAGACAGGCCATGCCTTTCAAGTGCATGCAGCTCAC490.1642476452250863No Hit
TCTGCAGGGGTAGACTCAGTTACCCAGTATGCCTTAATTTTTTAATGTGTGA490.1642476452250863No Hit
AAGGCTGGGACAAGGCCAATGTGGCCAAGGCTGGCTTGGTGGATGACTTTGA490.1642476452250863No Hit
TTTCAGGGGTCCTAAAAACAGACCATTTTCCTTAACTTGCATCAGTTTTGGT480.16089565246539067No Hit
TGTATAGGGCGCTCTTCCGATCTTTGCAGGGGTGCAAAAATCTATGAAGTTA480.16089565246539067No Hit
AACAAGGGCCGATCTGGCCACGGGCACTAAATTTATAACTTTTTGAAACAAA470.15754365970569503No Hit
GCATCAGGGCATCTTGATCTGTGCACTAGTCTGTGATTATTAGGGTGTTAGT460.1541916669459994No Hit
TGAGGGTGGCATTCTGCTGACCACAGCACTCCAACCAAAAGCCAAAAACCAA460.1541916669459994No Hit
TAACAGGGGCTTGTTTTACCTTGGCTCATTAGTGTTTAAAAATGTACTGATG460.1541916669459994No Hit
GCCATAGGGAGTTTCTGATGTTTCAGGCAGGAGTGGGGTAAATAAGTGTGAC450.15083967418630376No Hit
TACACTGGGGTGTCCGGGATGGATGGACAGCTTCTTTGCCCAAAATGCAGTG450.15083967418630376No Hit
CAGCAGTGGTATCAACGCAGAGTACGGGTGGCCCATCGCCATTGCAACGGCT440.14748768142660812Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TAGTATGGGATTACAGGCAAGCATGAAATGTAAAGTATTTATTTAAATAAAA430.14413568866691248No Hit
TATTCCGGGGTGGGCTTTCTGAGGCGATGCCCGTTGGGCAAACTTAGGTTTA430.14413568866691248No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTA420.14078369590721684No Hit
GATAAAGGGGAGGAGCTGGTGGCGTCGGCAGGTTCGAGGCGATTCGAGCTCC410.1374317031475212No Hit
TGGCTTGGGCGAGGAGCGCTGATGACGTGTGCTGTGGTCCTGTGGGTGCTGC410.1374317031475212No Hit
TTTTTTGGGATCTGTGACTATTATATAGGGATCACAAAAGTGTCACATATTA400.13407971038782554No Hit
TGTTGAGGGCTCTTTGCTTTGGTAAAGTACATTCACACTTAGGTCTAAGTTA390.1307277176281299No Hit
CATTTAGGGACTTTGGTTTAAAAACAAAAACCCTTATATACATGGAATAGTT390.1307277176281299No Hit
TTGTCAGGGACAAGTATACTTATTTCTTGGTGCGTAATCTTTACATGATGTC370.12402373210873864No Hit
GTTAGTGGGTCTTGGCAAGTTGCAGAGATGGTGGAGCTAGAAAAAAAAAAAA370.12402373210873864No Hit
CGGTTGGGGATTCTTGTATGTGTAAAATGTTAACCTAGCCATAAACATTAGC370.12402373210873864No Hit
TACTGAAGGGACTGGTGGTTTCTTAGTGGAAATTTTTCTTTCACTCTCACTT370.12402373210873864No Hit
CAGACAGGGATGTCCTTGACTACAACAGTAAAAAAATTGGAAACAACCTAAG360.120671739349043No Hit
TTTATGGGGAACAATGTATGTTTGGAGTTCCAAATCTGCAAGTGGCCAACCT360.120671739349043No Hit
TCTTGGGGGAGGACACAACTTATACAGTATAGGAAGCATTATACTTTTTTGA360.120671739349043No Hit
ATTAGCGGGTAACACCAAAAGTGAACCCAAATGTAAACTTAGGACTTTGAAG360.120671739349043No Hit
TTTGTAGGGGGGTCCCGGTGCGTGGGGCCCGGAACCAGCTGCGCATGTACCT350.11731974658934737No Hit
TCGCTAGGGAGGATGGCTAGGAAAGGGAAGCCTGACTGCTCGGTCAGGAGGG340.11396775382965174No Hit
TAAATTGGGACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA340.11396775382965174No Hit
TCAAGTGGGGAGGCCACTTCCGGCGTAGCCATGGCGGCTAACGCTACTACCA340.11396775382965174No Hit
TATAGTGGGTGAGATACCTTTTACAATGATTTAAGAAAAATCAATTCTGTTA330.1106157610699561No Hit
CTTAGTGCGGGATGTAGGACGCGGCATCCTGCCCCTGGTAGAGAGGACGAAT330.1106157610699561No Hit
TCGTTTGGGTATGAGAAATATTTAGACAGCATCTGTTTCTATTTTTAGACTG330.1106157610699561No Hit
GTAGGTGGGAGGGCTTGGGGAGAGGGAGGACACATCTGGAGGAAATGGCCTT320.10726376831026044No Hit
CCCCAGGGTACCTTTTCAGTAACATTTTATACATCTACTTGTCAATGTATTT320.10726376831026044No Hit
ATAGGGGGGGCCGAGCCACCTCGGATCCCACTGATTGGCCAGCCGAGCGAGA320.10726376831026044No Hit
GCTAAATACGGGTTATGGAAATCTTTAGCTTTTAATCATTTTTACCAATTTG320.10726376831026044No Hit
CTAGTGGGGGATTCAACCAGGTGTTGAAGGCACGACAGCCACTGCTCTACGA310.1039117755505648No Hit
TCAGAAGGGAAGTATTTTTTCATCACTGAAAGGTTTTTTTTTTTTATCACTA310.1039117755505648No Hit
CAGGATGGGGGGAATGGGAAGATACTGATCAAAGGATGAAAACTGTCAGTTA300.10055978279086916No Hit
TCTGCTGGGTCTTCCGATCTGCATGTGGGCAAAAGGTGAACTATTGGACACA300.10055978279086916No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)