FastQCFastQC Report
Sat 31 Dec 2022
SRR5844061.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844061.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86932
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC4700.5406524639948465No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT3930.45207748585101No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1570.18060092946210832No Hit
CTGAGGGGGGTGGCTCAGGGAAGCTCATACAGATGGGAACTGTCTTTCAGAA1290.14839184650071321No Hit
GTAATTGGGGATGGGATTTTATTGCTTTTTGTTTTAAAGGATGAAATTTTGT1280.1472415221092348No Hit
TTCGCCGGGAGCCCAGGGATTTGTCTCCTCCACCTGGACATACTACTTGCAG1270.1460911977177564No Hit
ACGGGCGGGCTATCACACACTGGTTGCCAATTATATGTCTGGGTTTCTCTCC1260.144940873326278No Hit
TAGCATGGGTAAAGTGAAGCTGCTTAAGGAAGTTTTCAGGCAATTTGTGGAA1230.14148990015184282No Hit
GTTTCTGGGATTGGGAAAGGACTTATATGGGGCAAAACTGATTGCACAAAAA1230.14148990015184282No Hit
AAGGCTGGGGATGCATAGTTTGCAGATATGTTCTCTTGTTCCGTAGGTTGTC1220.14033957576036443No Hit
ATTTAAGGGACAGTTTTTAGTCAGTTGACAGCCATATAATTCTTAGTTTTAT1220.14033957576036443No Hit
GCAAATGGGACTCAGAATGTTCTTAATATGGCTTCTTTCTTCTAATGATTGA1180.13573827819445083No Hit
CTTCACGGGATGAGTAGTCTAATAAGAACAGTTTTTTTCCATGTGAAGCAAC1170.13458795380297245No Hit
CTGCTAGGGGGACAGGGATAATGAGGAAAGACTGAGTCAGCTCTACAGGAAT1160.13343762941149404No Hit
GGTCATGGGGTTTTAGCTGTTAGGAAAAATATTCCTTGAAAGTATCAACCTT1160.13343762941149404No Hit
CTGACACAGGGGTGAATTCAAAGTTACAAATTGTTTCATTTCCTAGTGAAGT1160.13343762941149404No Hit
TTGAGAGGGTGCCAGGCCGAGGCTCAGCCCCAGACTCTCAGCATCTGCTGCC1160.13343762941149404No Hit
TTAGCCGGGGAGACAGCCTCAGAGCTGGTGGGGAGACAGCATGCCACTGGAA1150.13228730502001565No Hit
CATCTCGGGACAACAACCAATAATATTTACTGAATTCAAACTATTTACTTGT1150.13228730502001565No Hit
TGACTAGGGCTTGGAAAACGTGGTGCTTTGTCGCGAGTCTCAAGTGTCCATC1140.13113698062853724No Hit
CACCGGGGAGGCTAGATGTTTTTTAATATCTGGCCCCCTTTAGTCACCACTC1140.13113698062853724No Hit
ACGTGTTGGGTAGGCAAATCAATATAGAGGCTCCTCGAGAAATTGAAAATAG1130.12998665623705885No Hit
AATTCCGGGAGTAGCAAAGTCAAAGGTAAGAAGTTTATGAAATTGCCTTTTT1120.12883633184558047No Hit
ACAAGAGGGACAATAGGATGGGCTTCTGTGCCCAGAATACTGATGGAATTTT1120.12883633184558047No Hit
CTACCAAAACAATAAATAAATTAGCCGGGCGTGGTGGTGCGCATGTGTAGTT1110.12768600745410205No Hit
TGCAGCGGGAGCGTTTTTTATTTTTAATTCTTATAGAACAGCATAAAAATGT1110.12768600745410205No Hit
ATTACAGGGATGAGAAACCAGTAAGTACATCTCTCCATAGATCTTGACCTTG1110.12768600745410205No Hit
GTGTATGGGCGATCTGGAACGGGTAGGTAAGTGGTAGTAGCTCTGAAGTCAG1100.12653568306262367No Hit
CCACGGGGGTTCTCCCTGAGATGATGTCTTCAGTAATGATCGCACGTATTGG1100.12653568306262367No Hit
AAGGCTGGGGAGACAGGGCTTGTCTGGATACGTCATGGCCAGAGGCTCATCC1100.12653568306262367No Hit
GTGGATGGGCTGGATTGTTTGTAACACATAGGATAAATGCTGGAGGGGATGG1090.12538535867114528No Hit
TCGGTTCGGGATCTCGTAACAAATATAAATGACCCACATTTCACACTTAAAT1090.12538535867114528No Hit
TGCTATGGGAGGTATGCCACTTATTTTATTCATTTTTGTGTAAGGGAAATGA1090.12538535867114528No Hit
GCCGGGGGAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGAC1090.12538535867114528No Hit
TGTGGTGGGATTCTAGAGCCACACATTGAATCAGAATCTATCTTTATGAGTT1080.12423503427966685No Hit
AACACGGGGAGGCATTGAGGCAGCCAGAGCAGGGGCTTCTGCTGAGGGGGCA1080.12423503427966685No Hit
TCAACGGGGAGGAGGATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTAT1080.12423503427966685No Hit
GACATTGGGATCTAAGCAAGGGATGTGACAAATGTTCTTCCAAAGAACATTG1070.12308470988818847No Hit
ATTAACGGGAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCATCCTAGGGCA1070.12308470988818847No Hit
TATTTAGGGGAAGTAAAGTCATGAATCCTCACACTGTCAAATTTAATTAAAG1060.12193438549671007No Hit
TGTAGCGGGAGAGCACTTCCTTCCGCGGCTGCTAAAACTGGTGCGCGGGTTC1060.12193438549671007No Hit
TATCACGGGGGTTGAACATGCATTTTTCATGGAGTTTGGGTTCTAAATTTAG1050.12078406110523168No Hit
ACAGCCTTGGAGTTATCTTTTTCTGCTCTCCATTCTCCATGTCCAAAAACTT1040.11963373671375328No Hit
ACTCGAGGGAAGTGTCATGTCAGAAGTCAAAAAGTTTTGGATTTTAAAGATA1040.11963373671375328No Hit
ATCTGGGGGAGGGCGTCCTCTACGTCGGAAGCAAGACCCGAGAAGGTGTGGT1040.11963373671375328No Hit
GGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1040.11963373671375328No Hit
ACTCGAGGGGAACCCCAGGCTTTATATGTATACTTTGACCTCAGTGTTAATT1040.11963373671375328No Hit
GAAAATGGGCTATTTGGCTATGATACTTCGTCTTTTTTCTATGGTCAGTTGA1040.11963373671375328No Hit
GTAGGCATGGAGATCGAGACGGCGGTCACATTCTAAGTCTAGGAGTCGGCGA1040.11963373671375328No Hit
TACAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC1040.11963373671375328No Hit
TATTAAGGGGTGGCATGCACCCGCAGTCCTAACTACCCAGGAGGCTGAGTTG1040.11963373671375328No Hit
TAACGGCGGGAGTTTACAAAGAGCTTTTATACGTTCATCATCTTGCTTGATT1030.11848341232227488No Hit
TACTTATGGGACAGCAGAGGCTGATCAAATTCTTCATTGACCAGAGTAAAAA1030.11848341232227488No Hit
TTGTAGGGATCTTCAGACAATACTCTCCAACACCAGTCTTTGTGTAGTTTCC1030.11848341232227488No Hit
AATTCAGGGTAGCCTTGAACAACCAGATGGTGACCCAGAACAGGACTCCACA1030.11848341232227488No Hit
AAGCGGGTGGCAGAGCTGGAATCTGAACTCTGATAGAATTTTAAAAACTAAT1030.11848341232227488No Hit
GCCGCGGGGTCCTGTCAGCCCGGCAGTGTCTTCTCAGTGTCTTCACCTGAGC1020.11733308793079647No Hit
CATCTAGGGCACAAGAGACTCTAGAACTTTTTGGAGGCGTCCATGGGGAGTC1020.11733308793079647No Hit
TATACGGGGAGCAACACTTCCAAGCCCTTTTGTCAACTTGGTTAACCATCTC1020.11733308793079647No Hit
ATGTTGGTTTACCTGTAGTGTTTTATAAAGTGTGGTCAGAGGCCCCTATAGA1010.11618276353931808No Hit
GGTCAAGGGGTATGAGGGAAGGAGGTAGACACTAGTCAAGAAAAGCAGCACA1010.11618276353931808No Hit
TTACTAGGGATGCTGCTGTATCCTCAGTTGACTCATTCACTCAGTTCACACA1010.11618276353931808No Hit
CACAACGGCTGTCATTGATGTTGCATTTCCAAGGAGATAATCTCCTTCTTGG1000.11503243914783969No Hit
ATGTAAGGGATTGCAAACTATTCTAGAAGGCTCAGTTTAGTATGGGATTGTG1000.11503243914783969No Hit
CCAATAGGGAGAGTTATGTGAAATCCTAGCTAAAGAAAAGGACACTATAAGA1000.11503243914783969No Hit
TAGATTGGGAATCTAGAATGAAAAAGATAAAACCAAGGACAAGTGAATGTGG1000.11503243914783969No Hit
GAGGCGGGAGGATCCCTTGAGCCCTGGAGTTGGAGGCTGTAGTGAGCTATGA1000.11503243914783969No Hit
GTCACTGGAGTTGCTTGGTCCTCAGAAGAGGCCTGGGAAAGTAGTAGTGCTA1000.11503243914783969No Hit
ATTGACGGGACTGCAGGACTTAATCTGTAGTCTTTAGACAACAGTTGCTTTC990.1138821147563613No Hit
CCGTCGAGGGGGTGCCTGGCCCTAATTTTCATTTATTATACTTCCCTTACTA990.1138821147563613No Hit
AAGTGGGGCATCTAACTAGCTATATAGCAAACCAAACCCCTCTGAACCTGAT980.1127317903648829No Hit
CACAGTGGGATCACTGAAACAAAGAGGGGAGGAGAAGCATAGTAGCATGTTG980.1127317903648829No Hit
GTCTGCGGGCTCCCAAAGTCCTGGGATTGCAGGTGTGAGCCACCGGTCCCGG980.1127317903648829No Hit
AGTCCTGGCAGTTGTGTGATTAAAATGGCACCCTGATCTTCACCTGATTAAA980.1127317903648829No Hit
AACTTCGGGATAATAGACCAAAGGAATTTAACAGATATATACAGACCTTTAT980.1127317903648829No Hit
CTAACGGGGCTGCCCATCTGATAGGAAGAAACAGCCTTTTCTTTAAGGTACA970.11158146597340451No Hit
TACTGAGGGAATCTTTTTGGGGTATAGGAAAACTGTAATAGACCTTCTACCT970.11158146597340451No Hit
GTCAGTGGGATTCCTCTTCATAATGCATGCTCTTTTGGTCATGCTGAAGTAG970.11158146597340451No Hit
AGATGTGGGATGTTCTCACCTAATGGAATTTGCTTAAAAATATCCACTGAGG970.11158146597340451No Hit
CGGAACGGGACCAAGTGATTAAATTATACGGTTAGAGAAAAAATATTTTTAG960.11043114158192609No Hit
TTGATTGGGAGAATGAAAAGTAGCTTTTGAGTGACTTTAATTGTCAAAACTA960.11043114158192609No Hit
ATCACTGGGATGCTCTTAATGATTTACAGACTGATGCCAGACAAACCTTGGG960.11043114158192609No Hit
CAGCCAGGGCAGAAAACACTGCTCAGTGATTCACTTGCAGACAATTCAGGAA960.11043114158192609No Hit
TTTCCAGGGGATAAGGACTCCTTAAAGTTCCATTTGCTAATGATTAATACAC960.11043114158192609No Hit
GCTGGTGGGACCTGGCTTAGCCTCATTCCTGCTCGTAAGTCAGGCATTCAGC950.1092808171904477No Hit
ACAAGCGGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTC950.1092808171904477No Hit
GGCACGGCGGCCGCGTGTCCCTCCTTACAGGGGATGAAATGACCTGGGGAGG950.1092808171904477No Hit
TAACCAGGGCTGTTGTAGGGAGGATGTGAGTGAGCTAATTCTTTTGAGTTAC950.1092808171904477No Hit
TTATCCGGGGGTCCTGCTTGACATTGTATTGGAGCCTTACAGTTTCAAGACT950.1092808171904477No Hit
CGATTAGGGGACCCCTCAGCAGCAGGTAGAGGGTACTCTGTGCTGAGCCCTG950.1092808171904477No Hit
GGCGCAGGGAGGAAGCATTTCCCCGATAAGTTTTATAGTTTTAGCTCTTACA950.1092808171904477No Hit
ACACTAGGGTAAATGAGAAGGCCATAAAGATTCATTTAATAGCTGAAAAGCA940.1081304927989693No Hit
CAAAGTGGGATGAAAGCGAAAGGGAAAATAAAGAAAAACCAATAAAAAAGAG940.1081304927989693No Hit
GTGCTTGGGATAGCAGAAAACCTTTTATGTAGGCTTGCTTAGTCAACAACTT940.1081304927989693No Hit
TGAACTGGGAGGAGGTAGCCCATCTGTTGTCAAATAATCCGAGTTGTAGAAA940.1081304927989693No Hit
AAAGGCTGGGAAGATAAAACATTTTAATGCTAAAAACATAGGCCAAGCACTA940.1081304927989693No Hit
TCAACAGGGCATGGCTACATTCTAGACATTGAATTTATATCTTCTTTTTTTC930.10698016840749092No Hit
AAAGCTGGGCGTAGGGCTCTGGAAGGGATTAATAAAATAATATGAGTAAAGC930.10698016840749092No Hit
AAGCGGGGTAAGAAAAGCAAGACACAAGAAAGTGTTTCTAGCATGCTATCTT930.10698016840749092No Hit
GTTATGGGGGTTCAGTTCCTTTAATATTTACATTGCATATTGAAATCTTGCC930.10698016840749092No Hit
ATTGTAGGGCTTTGGGTTCAGCTGGGACAGCTCACAAAACTGAGGAAAACAC920.10582984401601252No Hit
GGCATTGGGCAGTACACCCAGTTGCCAAAGTTTTGCTGTCTCTTAGAGAAAG920.10582984401601252No Hit
GTTTCAGGGATGTAAAATGTAAAATGTCTTGTTTTCTCAGAGAACTCAACTC910.10467951962453413No Hit
ATATGGGCTCTTGGCTTAATTTCACCATACGATCTGTCTTGAAAATGCTTAT910.10467951962453413No Hit
GTATTTGGGTACTTGTCATGGCTTTTGTACGCAACTGTGTTTTAAAAATACC910.10467951962453413No Hit
ACCCTAGGGACCTGTGGTTTTTGGAAAACATTTCTAGCTTGGGGAATGTGAC910.10467951962453413No Hit
ACAGCGCGGGGACCTGCTCTTCCTTGCCTTCCACCATGATTGTGAGGCTTCC910.10467951962453413No Hit
CAGCAGGGTGAGACAAGCTAAAGAAGGTGCCTGAGGGAAGAACGTGAAGTGG910.10467951962453413No Hit
CATGCAGGGACAGCAGAATTCAGAACAAGGAAGGTTACCAGGCATGAAAAGT910.10467951962453413No Hit
ACTCTCGGGGAGGAAAAGCTGACATAGCAACCCAAGAAAGCTAAATCCTAGG910.10467951962453413No Hit
CGCATTGGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGG900.1035291952330557No Hit
TACCTTCGGGTATAATTTTGGCGAGGTTCATGGTCTTCCATTTCTTTTTCTT900.1035291952330557No Hit
AATGTAGGGCATGGCAGTGATTCTGTGGAGAGTGCAGAGAAGGAGATCGGCT900.1035291952330557No Hit
GACTTTGGGCTCCTTTTTAAAGGGTGAATCATATTCCATTATTTATATAAAC900.1035291952330557No Hit
TGATATGGGGGGAAGTGATCCCCTGCGTGGCTGGGCTGCTCGGGTTAGATCG900.1035291952330557No Hit
CGAATGGGGCTTTTACATACTGGCCCATGTATTTTTCAGTAACAAGCTATTC900.1035291952330557No Hit
TTCCGTGGGCTACGCATTGTCCTTAAAGCAAACAAATGAACTTGCCCCCAAG900.1035291952330557No Hit
CAGTGGGGGTAATGAGCATGAACTCAAGCAGTGGTACAAAGGATTTCTCAAG900.1035291952330557No Hit
GTGCTGGGGAACAATCAGTGAAGCAGTCAGAACATATACAACATTTATAGGT890.10237887084157732No Hit
ATATGCGGGACAGCATTGAAGCAGTTGCTAATGAAGTATTGAAAATGGAGAT890.10237887084157732No Hit
CTTCAGGGACAGGAAATAAAAACCAAAACAACTCAGACAGAGAAGACTGGAA890.10237887084157732No Hit
TGCAGCTGGGGTACATACATGATGGTGTGTGTTTATACACAAAGGCCTGAAT890.10237887084157732No Hit
GTTTGCGGGTCAGTAGGACTTCTAATGTAATAAATCAGGCAATATAATGTTA890.10237887084157732No Hit
ATCACAGGGATCATTAATTATGGACTCACATGAATGCATGTCTACATGTCGT890.10237887084157732No Hit
TTCGAGCGGGCTGTGCAATTTTGGGCACGTTATTCAATATCAGTTTTCTCAT890.10237887084157732No Hit
GTATTCGGGAGTCCGGGCTAAGCCAACAGATGCTCTTCTCCCTGGCAGAAGT880.10122854645009892No Hit
GGCTCCGGGACCTCCCAAATCAATGGTAGCTTTCCCAAATGAACACAAGTAT880.10122854645009892No Hit
GATTTAGGGGAACAATGACAACCAATGCACAGCTGCCCACAAGGTGGGCTCC880.10122854645009892No Hit
CGAATAGGGAGACCAATGATGAGTATTCTGGGGTGTCTGAATCAATGATTTT880.10122854645009892No Hit
GTTTGTGGGACCTGTGAGCATGGGAATACAAAAGGTTTGAGATTCCTGTAAA880.10122854645009892No Hit
CAAGATGGGTCTTTCCGAGGGGAGGTGAGGTGGCTTTCTCGAGGTAAAGTTC880.10122854645009892No Hit
GTGTGGGGGATTCATAGAAAACTGGTAAATGTGTTTCCCTGAGTACTTTGAG880.10122854645009892No Hit
CACAAAGGGATGTTTGATTTTTAAACTATTTAGTACTAATAGTTGAGATGAA880.10122854645009892No Hit
GCTCTTGGGGGCATGCGCAGCGCGGAGTCCCGGCCCGGGACACAAGATGGCG870.10007822205862053No Hit
CTTTAAGGGGGGCCCCTGACGGGGTGGCGGGTGTTCCTGCTGCTGCTGTGCG870.10007822205862053No Hit
CTACACGGGAAATCTGGGGTAGGACCAAAAGTTTTGCATTTTCAACAAGTTT870.10007822205862053No Hit
GCTTGTGGGTACTACAGGAAATCACAAAGCTCTATTATGTACCAAAACAGCA870.10007822205862053No Hit
TGACCTGGGGGTATAAATGAATGAAATTATGAGGTAATTGTATTTTTGTAAC870.10007822205862053No Hit
CAGGTGGGGAAGCCAGACATTTTCACAATTGTGAAGAAATAGATGTTTTGTT870.10007822205862053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)