FastQCFastQC Report
Sat 31 Dec 2022
SRR5844060.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844060.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87220
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC4390.5033249254758083No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT3760.4310937858289383No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1610.18459069020866772No Hit
GTAATTGGGGATGGGATTTTATTGCTTTTTGTTTTAAAGGATGAAATTTTGT1370.1570740655812887No Hit
CACCGGGGAGGCTAGATGTTTTTTAATATCTGGCCCCCTTTAGTCACCACTC1280.14675533134602156No Hit
GGTCAAGGGGTATGAGGGAAGGAGGTAGACACTAGTCAAGAAAAGCAGCACA1270.14560880531988077No Hit
ATGTCTGGGGAGTCAGATGCTGTGGTACTTGGCATTTAACAACTAGAATTCA1270.14560880531988077No Hit
AATTCAGGGTAGCCTTGAACAACCAGATGGTGACCCAGAACAGGACTCCACA1250.14331575326759916No Hit
ACGTGTTGGGTAGGCAAATCAATATAGAGGCTCCTCGAGAAATTGAAAATAG1240.14216922724145836No Hit
TTAGCCGGGGAGACAGCCTCAGAGCTGGTGGGGAGACAGCATGCCACTGGAA1220.1398761751891768No Hit
GTGTGGGGGATTCATAGAAAACTGGTAAATGTGTTTCCCTGAGTACTTTGAG1220.1398761751891768No Hit
GTGCTTGGGATAGCAGAAAACCTTTTATGTAGGCTTGCTTAGTCAACAACTT1210.138729649163036No Hit
CAATAAGGGGATCTGGATCTGGGACCAAGTGGAAGAATCTGCCTGCTATTTT1210.138729649163036No Hit
ATCACTGGGATGCTCTTAATGATTTACAGACTGATGCCAGACAAACCTTGGG1200.13758312313689522No Hit
TGACTAGGGCTTGGAAAACGTGGTGCTTTGTCGCGAGTCTCAAGTGTCCATC1200.13758312313689522No Hit
ATTAACGGGAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCATCCTAGGGCA1200.13758312313689522No Hit
TAGCATGGGTAAAGTGAAGCTGCTTAAGGAAGTTTTCAGGCAATTTGTGGAA1190.13643659711075443No Hit
GAGGCGGGAGGATCCCTTGAGCCCTGGAGTTGGAGGCTGTAGTGAGCTATGA1190.13643659711075443No Hit
TTGAGAGGGTGCCAGGCCGAGGCTCAGCCCCAGACTCTCAGCATCTGCTGCC1190.13643659711075443No Hit
AATGTAGGGCATGGCAGTGATTCTGTGGAGAGTGCAGAGAAGGAGATCGGCT1180.13529007108461363No Hit
CTTCACGGGATGAGTAGTCTAATAAGAACAGTTTTTTTCCATGTGAAGCAAC1180.13529007108461363No Hit
GCTTGTGGGTACTACAGGAAATCACAAAGCTCTATTATGTACCAAAACAGCA1180.13529007108461363No Hit
ACTCGAGGGGAACCCCAGGCTTTATATGTATACTTTGACCTCAGTGTTAATT1170.13414354505847284No Hit
TTCGCCGGGAGCCCAGGGATTTGTCTCCTCCACCTGGACATACTACTTGCAG1170.13414354505847284No Hit
TATCACGGGGGTTGAACATGCATTTTTCATGGAGTTTGGGTTCTAAATTTAG1160.13299701903233202No Hit
TGCAGCGGGAGCGTTTTTTATTTTTAATTCTTATAGAACAGCATAAAAATGT1140.13070396698005043No Hit
GGTCATGGGGTTTTAGCTGTTAGGAAAAATATTCCTTGAAAGTATCAACCTT1140.13070396698005043No Hit
AAGGCTGGGGATGCATAGTTTGCAGATATGTTCTCTTGTTCCGTAGGTTGTC1130.12955744095390964No Hit
GCAAATGGGACTCAGAATGTTCTTAATATGGCTTCTTTCTTCTAATGATTGA1130.12955744095390964No Hit
TGACCTGGGGGTATAAATGAATGAAATTATGAGGTAATTGTATTTTTGTAAC1130.12955744095390964No Hit
GAATGCGGGCAAAGGCAGATAGTAAAGATTAAAAAAAATCATGATCTTTGTG1120.12841091492776885No Hit
CTGACACAGGGGTGAATTCAAAGTTACAAATTGTTTCATTTCCTAGTGAAGT1110.12726438890162806No Hit
CCACGGGGGTTCTCCCTGAGATGATGTCTTCAGTAATGATCGCACGTATTGG1090.12497133684934648No Hit
CAGCCAGGGCAGAAAACACTGCTCAGTGATTCACTTGCAGACAATTCAGGAA1090.12497133684934648No Hit
GAAAATGGGCTATTTGGCTATGATACTTCGTCTTTTTTCTATGGTCAGTTGA1070.1226782847970649No Hit
AGCTATGGGGCTGCGGGTTGCGGGCTGCGGGCGCTAGGCTGCCCCGGGGAGG1070.1226782847970649No Hit
GCCGGGGGAAGGAGGAAGCGGGTAGTCGACCGTGTCCGCGCGCCTGGGAGAC1060.12153175877092409No Hit
CAAAGTGGGATGAAAGCGAAAGGGAAAATAAAGAAAAACCAATAAAAAAGAG1050.1203852327447833No Hit
GTCACTGGAGTTGCTTGGTCCTCAGAAGAGGCCTGGGAAAGTAGTAGTGCTA1050.1203852327447833No Hit
ACTCTCGGGGAGGAAAAGCTGACATAGCAACCCAAGAAAGCTAAATCCTAGG1050.1203852327447833No Hit
AGATGTGGGATGTTCTCACCTAATGGAATTTGCTTAAAAATATCCACTGAGG1040.1192387067186425No Hit
ACTCGAGGGAAGTGTCATGTCAGAAGTCAAAAAGTTTTGGATTTTAAAGATA1040.1192387067186425No Hit
TGTGGTGGGATTCTAGAGCCACACATTGAATCAGAATCTATCTTTATGAGTT1030.11809218069250171No Hit
GACATTGGGATCTAAGCAAGGGATGTGACAAATGTTCTTCCAAAGAACATTG1030.11809218069250171No Hit
CACAGTGGGATCACTGAAACAAAGAGGGGAGGAGAAGCATAGTAGCATGTTG1030.11809218069250171No Hit
AAGTGGGGCATCTAACTAGCTATATAGCAAACCAAACCCCTCTGAACCTGAT1020.11694565466636092No Hit
TGCTTAGGGACTGGAATTTCATCTGACACCTAATTTTTTCCAACTGGGGTTT1020.11694565466636092No Hit
ATATGCGGGATTCCCAATCATGACCTAATCACCTCCCAAAGGCCCCACCTCC1020.11694565466636092No Hit
CTTTTGGGGCCATGACAAGGGATGTGGAGCTTAAATGTTGTGAAGGGCTGTG1020.11694565466636092No Hit
GCGTAAGGGACTGCAATTTTCAGGAGACATATTCAACTCCTCTGCTCTTCCA1010.11579912864022014No Hit
AATTCCGGGAGTAGCAAAGTCAAAGGTAAGAAGTTTATGAAATTGCCTTTTT1010.11579912864022014No Hit
TCGGTTCGGGATCTCGTAACAAATATAAATGACCCACATTTCACACTTAAAT1000.11465260261407935No Hit
CGATTAGGGGACCCCTCAGCAGCAGGTAGAGGGTACTCTGTGCTGAGCCCTG1000.11465260261407935No Hit
AAGGCTGGGGAGACAGGGCTTGTCTGGATACGTCATGGCCAGAGGCTCATCC1000.11465260261407935No Hit
TATGTGGGGGATTGCACTGCCGACTGAGAGCTCCACTCCGCTGTCTGCCATC1000.11465260261407935No Hit
GCTCTTGGGTGTACCGGGACCGGAAACTTCTTGGTGCTGAAGAGAGGATCAT990.11350607658793856No Hit
ACAGCGCGGGGACCTGCTCTTCCTTGCCTTCCACCATGATTGTGAGGCTTCC990.11350607658793856No Hit
AATATAGGGCAATCTGTTGTGAAGAACCTGATTTGCACTCTGTAGTGTTTAA980.11235955056179776No Hit
GTATTTGGGTACTTGTCATGGCTTTTGTACGCAACTGTGTTTTAAAAATACC980.11235955056179776No Hit
GCTATGGGTCTCTACATTCCTGAAACAATTTTTAGGTTTTTTTTGTACTAGA980.11235955056179776No Hit
TAACCAGGGCTGTTGTAGGGAGGATGTGAGTGAGCTAATTCTTTTGAGTTAC980.11235955056179776No Hit
CTTGCTGGGAATTATGATCTACCTACCAATCGTGAAAACTATATTCACAGAA980.11235955056179776No Hit
CGTAATGGGGACGTTATGATTGTATATCTAGAAAACTCCAGGGTCTCAGACC980.11235955056179776No Hit
AACAAAGGGTTACAATATAAATTTTTATATTCATTGGAACTGTTTCAGAAAT980.11235955056179776No Hit
GACTTTGGGCTCCTTTTTAAAGGGTGAATCATATTCCATTATTTATATAAAC980.11235955056179776No Hit
GTTTCTGGGATTGGGAAAGGACTTATATGGGGCAAAACTGATTGCACAAAAA980.11235955056179776No Hit
CTGAGGGGGGTGGCTCAGGGAAGCTCATACAGATGGGAACTGTCTTTCAGAA980.11235955056179776No Hit
CGAATAGGGAGACCAATGATGAGTATTCTGGGGTGTCTGAATCAATGATTTT970.11121302453565696No Hit
CAAGATGGGTCTTTCCGAGGGGAGGTGAGGTGGCTTTCTCGAGGTAAAGTTC970.11121302453565696No Hit
AGTCCTGGCAGTTGTGTGATTAAAATGGCACCCTGATCTTCACCTGATTAAA970.11121302453565696No Hit
AAGCGGGTGGCAGAGCTGGAATCTGAACTCTGATAGAATTTTAAAAACTAAT970.11121302453565696No Hit
AACTTCGGGATAATAGACCAAAGGAATTTAACAGATATATACAGACCTTTAT970.11121302453565696No Hit
GAATACGGGCTGACCTAAGCTTGTGTGGTCTTTTGCTCTTCATTGGCAATGT960.11006649850951616No Hit
AATGGAGGGAGGAAATAACAGCCTTCATTTGTTTTATGCCACTCTATTTAAT960.11006649850951616No Hit
CATTAAGGGAAACCGAATTCCACAACAGATTAAAAAGATTATTTGTTATGAA960.11006649850951616No Hit
TGAACTGGGAGGAGGTAGCCCATCTGTTGTCAAATAATCCGAGTTGTAGAAA960.11006649850951616No Hit
AACTGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.11006649850951616No Hit
CATCTCGGGACAACAACCAATAATATTTACTGAATTCAAACTATTTACTTGT960.11006649850951616No Hit
ATATGGGCTCTTGGCTTAATTTCACCATACGATCTGTCTTGAAAATGCTTAT950.10891997248337537No Hit
TGCTATGGGAGGTATGCCACTTATTTTATTCATTTTTGTGTAAGGGAAATGA950.10891997248337537No Hit
TACCTTCGGGTATAATTTTGGCGAGGTTCATGGTCTTCCATTTCTTTTTCTT950.10891997248337537No Hit
TTGTAGGGATCTTCAGACAATACTCTCCAACACCAGTCTTTGTGTAGTTTCC950.10891997248337537No Hit
TCATATGGGATTAGAAAAAACTATTTTAAATTTCATATGGAACCAAAAAAGA950.10891997248337537No Hit
GTCTGCGGGCTCCCAAAGTCCTGGGATTGCAGGTGTGAGCCACCGGTCCCGG950.10891997248337537No Hit
CATGCAGGGACAGCAGAATTCAGAACAAGGAAGGTTACCAGGCATGAAAAGT950.10891997248337537No Hit
TTCCGTGGGCTACGCATTGTCCTTAAAGCAAACAAATGAACTTGCCCCCAAG950.10891997248337537No Hit
GGCACGGCGGCCGCGTGTCCCTCCTTACAGGGGATGAAATGACCTGGGGAGG940.10777344645723458No Hit
GTAGGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC940.10777344645723458No Hit
TACTGAGGGAATCTTTTTGGGGTATAGGAAAACTGTAATAGACCTTCTACCT940.10777344645723458No Hit
TATACGGGGAGCAACACTTCCAAGCCCTTTTGTCAACTTGGTTAACCATCTC940.10777344645723458No Hit
ACGGGCGGGCTATCACACACTGGTTGCCAATTATATGTCTGGGTTTCTCTCC940.10777344645723458No Hit
ACAAGAGGGACAATAGGATGGGCTTCTGTGCCCAGAATACTGATGGAATTTT940.10777344645723458No Hit
TGTAGCGGGAGAGCACTTCCTTCCGCGGCTGCTAAAACTGGTGCGCGGGTTC940.10777344645723458No Hit
TCAACGGGGAGGAGGATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTAT940.10777344645723458No Hit
GCTGGTGGGACCTGGCTTAGCCTCATTCCTGCTCGTAAGTCAGGCATTCAGC930.10662692043109379No Hit
TACTTATGGGACAGCAGAGGCTGATCAAATTCTTCATTGACCAGAGTAAAAA930.10662692043109379No Hit
GCTCTTGGGGGCATGCGCAGCGCGGAGTCCCGGCCCGGGACACAAGATGGCG930.10662692043109379No Hit
TTCGAGCGGGCTGTGCAATTTTGGGCACGTTATTCAATATCAGTTTTCTCAT930.10662692043109379No Hit
GCCATGGGTAAGCAATAGATTTTGCTTGGGTTTTCTTTTTTAAAAACATAAT920.10548039440495299No Hit
TGAATAGGGATTCCTATGCAGAGCCAAAACGAATAGTGTTGATAAAATAGCT920.10548039440495299No Hit
TTTACGGGAGTCTTCTTTGATCCTAAGACCACCACTGAAGTTGTTTAGGTTC920.10548039440495299No Hit
ATGTTGGGGCAGATGATCAGCAAAAGGAAGTGGTTAACATGGCCCAGAAGGC920.10548039440495299No Hit
CTTTGTGGGGAAGAGTTTCATATACACCAAATAATGTCTCATCTCTATAGTA920.10548039440495299No Hit
TCTATAGGGGTACAACACAGCAACTTCAGGGGCTCTTCAAGATTTCCCAGGT910.1043338683788122No Hit
CTACACGGGAAATCTGGGGTAGGACCAAAAGTTTTGCATTTTCAACAAGTTT910.1043338683788122No Hit
TTACTAGGGATGCTGCTGTATCCTCAGTTGACTCATTCACTCAGTTCACACA910.1043338683788122No Hit
GGCATTGGGCAGTACACCCAGTTGCCAAAGTTTTGCTGTCTCTTAGAGAAAG910.1043338683788122No Hit
CGTAATGGGTGAGTGGTCATTGTGTGACATCTTTCACTTAATAGAAAAGACT900.10318734235267141No Hit
GTGTATGGGCGATCTGGAACGGGTAGGTAAGTGGTAGTAGCTCTGAAGTCAG900.10318734235267141No Hit
CTAATGGGGAGAATGCTGCATGTGAGTACTCTAGATTGAGGGAATCCTTTAT900.10318734235267141No Hit
AAGCCTGGGTGACAGAGGAAGTCTCTGTCTGTCTGTCTGTCTCTCTCTCTCC900.10318734235267141No Hit
TTGTCTGGGTAAGAAGGGATTCTGTAGGCAATATATAGTTGAGTTTTGTTTT900.10318734235267141No Hit
GTAACAGGGAATTTAAAGAAAAATCTTGAAAGTAGCAGGAGAAAAATGACTT900.10318734235267141No Hit
TGACTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA900.10318734235267141No Hit
GCTCAGGGGCAATCCCGGGTTGAGCAAAATGGCGCGGGAGAAGGAGATGCAG900.10318734235267141No Hit
CTACATATGGGGAGGTACCCCCGCCGTCGCAGCCCCCGCGCTGGTGCAGCCA900.10318734235267141No Hit
CACAACGGCTGTCATTGATGTTGCATTTCCAAGGAGATAATCTCCTTCTTGG890.10204081632653061No Hit
CGGAACGGGACCAAGTGATTAAATTATACGGTTAGAGAAAAAATATTTTTAG890.10204081632653061No Hit
GTATTGGGGATGTTAAACAGAAGATACCTTTCTCCAAAATGGATCAAAATGT890.10204081632653061No Hit
TATAATGGGTGTTAAACACTTGGATCTCTCAATACTTCTGTAAGTTAAAGTG890.10204081632653061No Hit
TGAAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC890.10204081632653061No Hit
CGCGTAGGGGGCTCGGGCGGGCGGTCCGGCCCGGGACTCGGGTTTGGGCGAC890.10204081632653061No Hit
CACTGGGGGTGGCATTTGCTGAATAACAACAATTTAGGAATGCTCAATGTGC890.10204081632653061No Hit
GCTATGGGGATGACCCCTGGGTTTCTAGTATGAGCAAACTGATATGCCACCT890.10204081632653061No Hit
GCTATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC890.10204081632653061No Hit
GACCATGGGGTTATTACTTTGCACCAGGTGGTTTGGCCACTGAATTAATACT890.10204081632653061No Hit
AAATCAGGGGGGTCATCTATGGTCCCTTCCAACTTAAAACTTCTCTATTCAT890.10204081632653061No Hit
GTGCACGGGGATCCAGAGCAATGGGCAAGAAAAAGAAACAAAAGTCATCCAA890.10204081632653061No Hit
AAGTCGGGAGTCAGAGGGATCACAATCTTTCAGCTAACTTATTCTACTCCGA880.10089429030038981No Hit
TTGTCGGGGGCCTTTTTTTTCTGTAGAGAGCTTTTGGGAAGTGCCATTGTTG880.10089429030038981No Hit
CTACCAAAACAATAAATAAATTAGCCGGGCGTGGTGGTGCGCATGTGTAGTT880.10089429030038981No Hit
AATAGAGGGGTAGGGCTAGCATTTTTGCTGGGCCTTGAGGGATAAGGAAGAC880.10089429030038981No Hit
GTTTGTGGGACCTGTGAGCATGGGAATACAAAAGGTTTGAGATTCCTGTAAA880.10089429030038981No Hit
CCAATAGGGAGAGTTATGTGAAATCCTAGCTAAAGAAAAGGACACTATAAGA880.10089429030038981No Hit
GGTTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC880.10089429030038981No Hit
ATCTGGGGGAGGGCGTCCTCTACGTCGGAAGCAAGACCCGAGAAGGTGTGGT880.10089429030038981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)