FastQCFastQC Report
Sat 31 Dec 2022
SRR5844059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87538
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGC4660.5323402408097055No Hit
CTTATACACATCTCCGAGCCCACGAGACACCTCGTTATCTCGTATGCCGTCT3960.4523749685850716No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1450.16564234960817018No Hit
AATTCAGGGTAGCCTTGAACAACCAGATGGTGACCCAGAACAGGACTCCACA1380.1576458223857068No Hit
GGTCATGGGGTTTTAGCTGTTAGGAAAAATATTCCTTGAAAGTATCAACCTT1360.1553611003221458No Hit
TGCAGCGGGAGCGTTTTTTATTTTTAATTCTTATAGAACAGCATAAAAATGT1320.15079165619502388No Hit
TGCTATGGGAGGTATGCCACTTATTTTATTCATTTTTGTGTAAGGGAAATGA1280.14622221206790192No Hit
ACGTGTTGGGTAGGCAAATCAATATAGAGGCTCCTCGAGAAATTGAAAATAG1250.14279512897256047No Hit
TTAGCCGGGGAGACAGCCTCAGAGCTGGTGGGGAGACAGCATGCCACTGGAA1230.14051040690899952No Hit
TATACGGGGAGCAACACTTCCAAGCCCTTTTGTCAACTTGGTTAACCATCTC1220.13936804587721904No Hit
GTCTGCGGGCTCCCAAAGTCCTGGGATTGCAGGTGTGAGCCACCGGTCCCGG1210.13822568484543854No Hit
TTATCCGGGGGTCCTGCTTGACATTGTATTGGAGCCTTACAGTTTCAAGACT1210.13822568484543854No Hit
ACAGCGCGGGGACCTGCTCTTCCTTGCCTTCCACCATGATTGTGAGGCTTCC1190.1359409627818776No Hit
CGAATGGGGCTTTTACATACTGGCCCATGTATTTTTCAGTAACAAGCTATTC1190.1359409627818776No Hit
ATCACTGGGATGCTCTTAATGATTTACAGACTGATGCCAGACAAACCTTGGG1190.1359409627818776No Hit
TCGGTTCGGGATCTCGTAACAAATATAAATGACCCACATTTCACACTTAAAT1170.1336562407183166No Hit
ATGTAAGGGATTGCAAACTATTCTAGAAGGCTCAGTTTAGTATGGGATTGTG1160.13251387968653613No Hit
CTACCAAAACAATAAATAAATTAGCCGGGCGTGGTGGTGCGCATGTGTAGTT1150.13137151865475566No Hit
CACCGGGGAGGCTAGATGTTTTTTAATATCTGGCCCCCTTTAGTCACCACTC1150.13137151865475566No Hit
CTTTTGGGGCCATGACAAGGGATGTGGAGCTTAAATGTTGTGAAGGGCTGTG1140.13022915762297518No Hit
ACTCGAGGGGAACCCCAGGCTTTATATGTATACTTTGACCTCAGTGTTAATT1130.12908679659119468No Hit
GATCTTGGGTAGGAAAAAGAAGAAGGTTTTCAAAAGGCAAGTGCTATTTTTT1120.1279444355594142No Hit
GGTCAAGGGGTATGAGGGAAGGAGGTAGACACTAGTCAAGAAAAGCAGCACA1120.1279444355594142No Hit
CCACGGGGGTTCTCCCTGAGATGATGTCTTCAGTAATGATCGCACGTATTGG1120.1279444355594142No Hit
ACGGGCGGGCTATCACACACTGGTTGCCAATTATATGTCTGGGTTTCTCTCC1120.1279444355594142No Hit
GTAATTGGGGATGGGATTTTATTGCTTTTTGTTTTAAAGGATGAAATTTTGT1120.1279444355594142No Hit
ATTAACGGGAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCATCCTAGGGCA1110.1268020745276337No Hit
TATCACGGGGGTTGAACATGCATTTTTCATGGAGTTTGGGTTCTAAATTTAG1100.12565971349585323No Hit
ATGTCTGGGGAGTCAGATGCTGTGGTACTTGGCATTTAACAACTAGAATTCA1100.12565971349585323No Hit
TTCTGTGGGAGGGCCAGTTGTGGGGTTGGTAAGCACAGTTATGAAAATTTCT1100.12565971349585323No Hit
GTTTCTGGGATTGGGAAAGGACTTATATGGGGCAAAACTGATTGCACAAAAA1100.12565971349585323No Hit
ACTCGAGGGAAGTGTCATGTCAGAAGTCAAAAAGTTTTGGATTTTAAAGATA1090.12451735246407274No Hit
TATTTAGGGGAAGTAAAGTCATGAATCCTCACACTGTCAAATTTAATTAAAG1090.12451735246407274No Hit
CTGACACAGGGGTGAATTCAAAGTTACAAATTGTTTCATTTCCTAGTGAAGT1090.12451735246407274No Hit
AGCTATGGGGCTGCGGGTTGCGGGCTGCGGGCGCTAGGCTGCCCCGGGGAGG1090.12451735246407274No Hit
CAAAGTGGGATGAAAGCGAAAGGGAAAATAAAGAAAAACCAATAAAAAAGAG1090.12451735246407274No Hit
AAGGCTGGGGATGCATAGTTTGCAGATATGTTCTCTTGTTCCGTAGGTTGTC1090.12451735246407274No Hit
GTCACTGGAGTTGCTTGGTCCTCAGAAGAGGCCTGGGAAAGTAGTAGTGCTA1080.12337499143229226No Hit
TGACTAGGGCTTGGAAAACGTGGTGCTTTGTCGCGAGTCTCAAGTGTCCATC1070.12223263040051179No Hit
CAGTAAGGGTATAGTGTTCAAGGCCAAAAATATTAACAATAAAGGCCAAGGA1070.12223263040051179No Hit
TCAACGGGGAGGAGGATAATGAAAAGTCCGTGTTTTGAAGTTTTCAGATTAT1070.12223263040051179No Hit
TTGAGAGGGTGCCAGGCCGAGGCTCAGCCCCAGACTCTCAGCATCTGCTGCC1060.1210902693687313No Hit
GCTTGTGGGTACTACAGGAAATCACAAAGCTCTATTATGTACCAAAACAGCA1050.11994790833695082No Hit
AATGTAGGGCATGGCAGTGATTCTGTGGAGAGTGCAGAGAAGGAGATCGGCT1050.11994790833695082No Hit
ATGTTGGTTTACCTGTAGTGTTTTATAAAGTGTGGTCAGAGGCCCCTATAGA1040.11880554730517032No Hit
CGCATTGGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGG1040.11880554730517032No Hit
GTATTTGGGTACTTGTCATGGCTTTTGTACGCAACTGTGTTTTAAAAATACC1040.11880554730517032No Hit
AATTCCGGGAGTAGCAAAGTCAAAGGTAAGAAGTTTATGAAATTGCCTTTTT1040.11880554730517032No Hit
CAAGATGGGTCTTTCCGAGGGGAGGTGAGGTGGCTTTCTCGAGGTAAAGTTC1040.11880554730517032No Hit
TGCTTAGGGACTGGAATTTCATCTGACACCTAATTTTTTCCAACTGGGGTTT1040.11880554730517032No Hit
AGATGTGGGATGTTCTCACCTAATGGAATTTGCTTAAAAATATCCACTGAGG1030.11766318627338984No Hit
GCAAATGGGACTCAGAATGTTCTTAATATGGCTTCTTTCTTCTAATGATTGA1030.11766318627338984No Hit
CTTGCTGGGAATTATGATCTACCTACCAATCGTGAAAACTATATTCACAGAA1030.11766318627338984No Hit
AAGTGGGGCATCTAACTAGCTATATAGCAAACCAAACCCCTCTGAACCTGAT1030.11766318627338984No Hit
TACAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC1030.11766318627338984No Hit
ACTAGAGGGGGTGATGCTATAGAGGAAAAAACCCGCAATTACTTTTGCACCA1030.11766318627338984No Hit
AAAGGCTGGGAAGATAAAACATTTTAATGCTAAAAACATAGGCCAAGCACTA1030.11766318627338984No Hit
ATTGACGGGACTGCAGGACTTAATCTGTAGTCTTTAGACAACAGTTGCTTTC1020.11652082524160935No Hit
CCAATAGGGAGAGTTATGTGAAATCCTAGCTAAAGAAAAGGACACTATAAGA1020.11652082524160935No Hit
CTACACGGGAAATCTGGGGTAGGACCAAAAGTTTTGCATTTTCAACAAGTTT1010.11537846420982888No Hit
TACCTTCGGGTATAATTTTGGCGAGGTTCATGGTCTTCCATTTCTTTTTCTT1010.11537846420982888No Hit
ATTTAAGGGACAGTTTTTAGTCAGTTGACAGCCATATAATTCTTAGTTTTAT1010.11537846420982888No Hit
CTTCACGGGATGAGTAGTCTAATAAGAACAGTTTTTTTCCATGTGAAGCAAC1010.11537846420982888No Hit
TGTAGCGGGAGAGCACTTCCTTCCGCGGCTGCTAAAACTGGTGCGCGGGTTC1010.11537846420982888No Hit
TGTACAGGGCATTCTTCATGTCATTAAGCAATAGGTTGCAGCAAACAAGGAA1000.1142361031780484No Hit
TCCATCGGGGTATCTTAATGTAATTAATCGTTACAAAATATATTATTATTAC1000.1142361031780484No Hit
CACAAAGGGATGTTTGATTTTTAAACTATTTAGTACTAATAGTTGAGATGAA1000.1142361031780484No Hit
CGGAACGGGACCAAGTGATTAAATTATACGGTTAGAGAAAAAATATTTTTAG1000.1142361031780484No Hit
CTAATGGGGAGAATGCTGCATGTGAGTACTCTAGATTGAGGGAATCCTTTAT1000.1142361031780484No Hit
ACAAGAGGGACAATAGGATGGGCTTCTGTGCCCAGAATACTGATGGAATTTT1000.1142361031780484No Hit
TGGGTTGGGATTAAAAGGAGATGTGAGAAGAAAGAATATTTGTAGAATGGTG990.1130937421462679No Hit
TCTATAGGGGTACAACACAGCAACTTCAGGGGCTCTTCAAGATTTCCCAGGT980.11195138111448744No Hit
GACATTGGGATCTAAGCAAGGGATGTGACAAATGTTCTTCCAAAGAACATTG980.11195138111448744No Hit
GTGGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC980.11195138111448744No Hit
GACTTTGGGCTCCTTTTTAAAGGGTGAATCATATTCCATTATTTATATAAAC980.11195138111448744No Hit
ATCTGGGGGAGGGCGTCCTCTACGTCGGAAGCAAGACCCGAGAAGGTGTGGT970.11080902008270693No Hit
AAATCAGGGGGGTCATCTATGGTCCCTTCCAACTTAAAACTTCTCTATTCAT970.11080902008270693No Hit
TTTACGGGAGTCTTCTTTGATCCTAAGACCACCACTGAAGTTGTTTAGGTTC960.10966665905092646No Hit
GTTGCCGGGGATTGCTTGAGAATTTTCCTTTCAAAATGATAATATCACACTT960.10966665905092646No Hit
AAACGGGAGTTTATGCAACTTAAAATGTTTACACACTGTGGTGCAATCTACT960.10966665905092646No Hit
GTAACAGGGAATTTAAAGAAAAATCTTGAAAGTAGCAGGAGAAAAATGACTT960.10966665905092646No Hit
GCGTAAGGGACTGCAATTTTCAGGAGACATATTCAACTCCTCTGCTCTTCCA960.10966665905092646No Hit
ATTACTGGGAGGAATGTTCAACCTATAAATTTTGTATCTGTCATTACGTTTA950.10852429801914597No Hit
TCATCAAGGGCTTTCATATTAATGGGATGAAAATAAAAATTTCATAATTATA950.10852429801914597No Hit
GTGTGGGGGATTCATAGAAAACTGGTAAATGTGTTTCCCTGAGTACTTTGAG940.10738193698736549No Hit
GAAAATGGGCTATTTGGCTATGATACTTCGTCTTTTTTCTATGGTCAGTTGA940.10738193698736549No Hit
CTGAGGGGGGTGGCTCAGGGAAGCTCATACAGATGGGAACTGTCTTTCAGAA940.10738193698736549No Hit
ATGTTGGGGCAGATGATCAGCAAAAGGAAGTGGTTAACATGGCCCAGAAGGC940.10738193698736549No Hit
GGTTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC930.10623957595558502No Hit
GCTGGTGGGACCTGGCTTAGCCTCATTCCTGCTCGTAAGTCAGGCATTCAGC930.10623957595558502No Hit
GTGGATGGGCTGGATTGTTTGTAACACATAGGATAAATGCTGGAGGGGATGG930.10623957595558502No Hit
CGAATTGGGTGGGGAGGGATCACTCAGGAAAACAGGAAAGGAGGTTCCAGCC930.10623957595558502No Hit
TGCCAGCAATTTGCAGTGGCCTAGTTGTCCCACACAATACTCTGAGCTTCAG930.10623957595558502No Hit
CTGATTGGGAATCTACATCTTGCTGCGTTCTGTTTGCTGAGGTTGGGCTTAT930.10623957595558502No Hit
GCTCTTGGGGGCATGCGCAGCGCGGAGTCCCGGCCCGGGACACAAGATGGCG930.10623957595558502No Hit
TTTAAAGGGAAGAGTAGAAATTATGGTCATAGAAATATGAAAACTCATATGC930.10623957595558502No Hit
CTATCGGGGCACTTTGTGTTATGTGGAGGAAATGTGTATTACCAATGGGGTT920.10509721492380451No Hit
ACAGCCTTGGAGTTATCTTTTTCTGCTCTCCATTCTCCATGTCCAAAAACTT920.10509721492380451No Hit
AAGTCGGGAGTCAGAGGGATCACAATCTTTCAGCTAACTTATTCTACTCCGA920.10509721492380451No Hit
GTGCTGGGGAACAATCAGTGAAGCAGTCAGAACATATACAACATTTATAGGT920.10509721492380451No Hit
TATAACGGGAAGGTGAAGGGTTCAGTATAAGTTGGAAATCCTAGAGCAACCA920.10509721492380451No Hit
ATTGTGGGGAGGCTGCGGTGAGCTATGAGTGCACCAGTGTATTCCAGCTGCA920.10509721492380451No Hit
TGACCTGGGGGTATAAATGAATGAAATTATGAGGTAATTGTATTTTTGTAAC920.10509721492380451No Hit
CGATTAGGGGACCCCTCAGCAGCAGGTAGAGGGTACTCTGTGCTGAGCCCTG920.10509721492380451No Hit
ATTAAGGGGAGCGTTGACATTTTTACTGAGGAGATCATCTCAAAGGTGATGC920.10509721492380451No Hit
TCCAATGGGATGTCTGTAGTTTGAAATAGAAGGTTAATTTTTTCCACTGGCT920.10509721492380451No Hit
GTGCACGGGGATCCAGAGCAATGGGCAAGAAAAAGAAACAAAAGTCATCCAA920.10509721492380451No Hit
GACCATGGGTCTGTAACCAACCTGAGTCAAACAGATGAAATCCCTGTATTGA920.10509721492380451No Hit
TGGCTTGGGGGAAGTCAGTGTGGCGATTCCTCAGGGATCTAGAACTAGAAAT910.10395485389202404No Hit
GTGTTCGGGAACAAATCTAAGAGTTATTGGCCTTAAAGAGGAGGTAGAGGGA910.10395485389202404No Hit
AAACCTGGGCTTCATATAGCCTTGGTAAATTTCAAGCTCTATATTTAATTAT900.10281249286024355No Hit
ACACCTGGGAGGCATTGAGGCAGCCAGCGCAGAGGCTTCTGCTGAGGGGGCA900.10281249286024355No Hit
GGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG900.10281249286024355No Hit
TAACCAGGGCTGTTGTAGGGAGGATGTGAGTGAGCTAATTCTTTTGAGTTAC900.10281249286024355No Hit
TTCGCCGGGAGCCCAGGGATTTGTCTCCTCCACCTGGACATACTACTTGCAG900.10281249286024355No Hit
AAGCGGGTGGCAGAGCTGGAATCTGAACTCTGATAGAATTTTAAAAACTAAT900.10281249286024355No Hit
TTTTGTGGGTTGGCAGGGGCAAATGATAGAACAGGGAATGAAAAGCAACAGC900.10281249286024355No Hit
TTGTAGGGATCTTCAGACAATACTCTCCAACACCAGTCTTTGTGTAGTTTCC900.10281249286024355No Hit
AAAGCTGGGCGTAGGGCTCTGGAAGGGATTAATAAAATAATATGAGTAAAGC900.10281249286024355No Hit
TTGTCTGGGTAAGAAGGGATTCTGTAGGCAATATATAGTTGAGTTTTGTTTT890.10167013182846307No Hit
GTTTACGGGAGATATTCAAGATTCCCATTGGTGGAAAGCTGGGGTCCTTAGT890.10167013182846307No Hit
ATCTTGGGGGAACATGGCGGCGGTGGTAGCTGCTACGGCGCTGAAGGGCCGG890.10167013182846307No Hit
CGAAAAGGGAAAAAAACATTCTATCTACTGATTTGGGCTGAATGTATGTAAA880.10052777079668257No Hit
GTTGAGGGGTCCGATCTTAATGGAGGGGTGACTAATAGGGATAATCCAGGTA880.10052777079668257No Hit
CTGCTAGGGGGACAGGGATAATGAGGAAAGACTGAGTCAGCTCTACAGGAAT880.10052777079668257No Hit
TTGATTGGGAGAATGAAAAGTAGCTTTTGAGTGACTTTAATTGTCAAAACTA880.10052777079668257No Hit
TGTGGTGGGATTCTAGAGCCACACATTGAATCAGAATCTATCTTTATGAGTT880.10052777079668257No Hit
CTTATGGAGGGACAACATATTATTCCATAAAACCAGTTTAGGGCACAGGCCA880.10052777079668257No Hit
ATTACAGGGATGAGAAACCAGTAAGTACATCTCTCCATAGATCTTGACCTTG880.10052777079668257No Hit
TTGTGGGGGACATGTACCTAAGGCTGCCAAAATGTGCTCTTGGACTGGTTAA880.10052777079668257No Hit
CATCTCGGGACAACAACCAATAATATTTACTGAATTCAAACTATTTACTTGT880.10052777079668257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)