FastQCFastQC Report
Sat 31 Dec 2022
SRR5844037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186272
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC4460.23943480501632022No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT4270.22923466758288952RNA PCR Primer, Index 17 (95% over 22bp)
TGACTTGGGTATCTGCAGGAATTTATTTTTTTACGTATTTTTACTAGTTGAT3250.17447603504552484No Hit
GTATGGAGGGGCTGAATTCTATCAGGCATTCAAAGAAGAATTGGTACTAATT2860.15353891084006185No Hit
CAACTAGGGAATAGAAAATGTTTAGAGTCAGGAGCACATTTCATAGTCCCCA2840.1524652121628586No Hit
GATGACGGGGGAGGAACATTGAGAAATTCACAAGTACATGGAAGTTAAATAA2820.15139151348565538No Hit
AAAAGAGGGGAAGTGGAAGAAACGCGGAACCATGGCCGCTGTGGTTGCTGTT2720.14602302009963924No Hit
TGCTTGGGGATCTCTACTAAAAATACAAAAATTAGCTAGGCATGGTGGCACA2610.14011767737502148No Hit
CATTCTGGGTGCTTTGTTTATGAAGCTGTGGGATACAAGGACTCTCATACGT2600.13958082803641986No Hit
AGTTTAGGGCTGTATCAGGGATAATGGAAATGGAAGGATATAATTATAAATG2570.137970280020615No Hit
CGCTCGGGGGTTGATGGAAGAGATGACCCATAGATGTCCCTACATCTCATTT2560.1374334306820134No Hit
GCAGTAGGGGAATAGGGCTTACTCACCCCTTCATGGAGGCTCGGGCAGCTAT2490.1336754853118021No Hit
ACTGCAGGGGACACGTCCCCTGGGAATAATCTCCACTCTAGTTTGAGGATGA2490.1336754853118021No Hit
AGCTGAGGGGCTGCTGGTTAACTTGAGTTTTAACACCTTTTTTTAAGTTCTC2460.13206493729599725No Hit
CGGCTTGGGTACAGAAAATGCACGTTTCCTGAAAACGTGGTGGGAACTCTTG2450.13152808795739565No Hit
AAGTCAGGGGGAGCTGCCAGTTGTGGGAGGCAGCAGCAAACAAGTAGATAAA2430.1304543892801924No Hit
CTAAGCGGGAAACAAAAACTTTCCATGACAGTAGCATACTGATGAGACAACA2420.1299175399415908No Hit
GCGTAAGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.12938069060298918No Hit
TTTCAGGGGCTAGAAAGCTATTAGGAAATCAAGGCATCTGGAGAAACTATGT2380.12777014258718433No Hit
TGCACTGGGAGAACATAGGAAGGGTCATGTGAAAAGTCAGCATGTCTGGAAT2380.12777014258718433No Hit
TACCTCGGGTGTTGATGCAGATGATGTGCGATTGGCAATCCAGTGCCGCGCT2370.1272332932485827No Hit
TCAGCAGGGATGTCCGAAATTGCCTGACTTCATGTTTTGGAAGATTCCTAAG2370.1272332932485827No Hit
AACCTTGGGGGGATTATTATTTTTCAAAGTTGTAGAATCCAATAATACAGTC2370.1272332932485827No Hit
TACGATGGGTACAGGAAATGGAAAAGCTTCCATGTGTTACCCTGTTTATGAA2360.12669644390998108No Hit
TGAAGCGGGAAGAGACACAGAGGAAATGATTTTGCCTTTAAGACACTCATGA2350.12615959457137949No Hit
ACATTAGGGATTTACAACTCAGTCATGGTGCTGCTGTCCTCCAGGCTTACTG2350.12615959457137949No Hit
TATGCCGGGATTTCAAATTATTATTATGCAACCACAAGGATCTCTTCTTTCT2350.12615959457137949No Hit
AAGCATGGGTCGAACGGGGGGAGTGGCGTGGCGTGGCGGGGTCTCGCGGCGC2340.12562274523277786No Hit
AAGCCGGGGATTGGTGTTAAGGTGGTAATTTCCCACATATAAGATTTAGAAT2330.12508589589417626No Hit
TGCAGAGGGATATAAAAACAAAACAACTGGTTCTGTGTGTATCAACTTAATT2320.12454904655557464No Hit
TATATGGGTCTTCTAGTGGGGGCTGAGCTCTACGAGGCGGAGCGGCGGCGGT2300.12347534787837142No Hit
TCCAATGGGGTTCTTTAATCATCATGATTCTGGTTTGTTGGATATCGGCAAA2290.12293849853976979No Hit
GTGTTGGGGACTATGGAAAATATATTGCGTACGGTCTTCTGATTTCTAGTTT2290.12293849853976979No Hit
AATACCGGGATTGGGCGGTGTCTTGGTGGCATGCTTCAGGGCTCCCCTGTGT2270.12186479986256657No Hit
AAATGTGGGGGTAGAGGCTATGGCAAACTGGAGACTGAGTGCCTGTTCCTCA2260.12132795052396496No Hit
CAGTCTGGGGAAATGCCAAAACTTTCCAGGAAAAATAGTTTTATTATCAATC2260.12132795052396496No Hit
GCACAAGGGGGCTTTGAACATGGGGTTGGCTGTTTCCCAGTAAAACTGGAAT2260.12132795052396496No Hit
GCTGTGTAGGGCTCTTTCTAACTTTTAGTAATTGTCAATAACACTTATATTA2240.12025425184676172No Hit
ATCTAAGGGATGAGGAACTGACCAATGATAGCCTGACCCTATCTCAAAGCAA2220.11918055316955849No Hit
CGTACCGGGATACTTAATGAGCAAATTGAAGTAAGCTTTTAAAGTATATTTC2220.11918055316955849No Hit
ACAGCTGGGGTTTTGGACATGTCTAAGAACCCAGGTATAGAATAATAAAGTT2210.11864370383095689No Hit
GACACTGGGCGATCTATGGTTGGGGTTCCTGTATATCAGTAGTACCAAAATT2200.11810685449235526No Hit
GCCACAGGTGCTGAGCCGTCCCCGGCCCTTCTGGTGCCAAGTGCCAAGCGCC2190.11757000515375364No Hit
GCAACTGGGATATGGAGCCCAGATAAATGAACTTCATTTGGCATACTGCCTG2190.11757000515375364No Hit
GGCTACGGGAATGAGCCTATTGGGTGGATTCAAAACTGATAAAATATTTGGA2190.11757000515375364No Hit
TATTGTGGGGGTTGACAATATATAGAAAACATCTGTGTATCACAAAATTTTT2190.11757000515375364No Hit
TTTGCAGGGGGGTGTTAGTTCCCGGTCACCTGAGCTCCGGGTGACGCGGCTG2190.11757000515375364No Hit
TCCGCTGGGATAGTAGGAAGAAAGGCTCATTTTTTTTTATGATTTAGAAAGG2180.11703315581515204No Hit
CCTTGCGGGGGCCGGCGCCAGGGCAGGCGGGCGGCTGGCAGCTGTGGCGCCG2180.11703315581515204No Hit
GGCACAGGGTGCAAAATACAACTTCACTAGCAGTTTTGAGTTTAATCCTGAA2180.11703315581515204No Hit
TGCTAGGGGCGATCTTTTCCCGGGACCTTTCAGAAATTTGTGTTCGAGTAAG2170.11649630647655042No Hit
GAATCTGGGATAGTGAAAATTGAAACTAGACTCCTTCCTTTCACCATACACA2170.11649630647655042No Hit
CTTAGTCAGGGATGAACAGACAATTCCCTAAGTTGTTTTGGTGCTTAGAATG2160.11595945713794881No Hit
AAAAGCAGGGGGTGCTCAGACAGCCTTAAACAAAGGTGGCTGAGAGTGAGGA2150.11542260779934718No Hit
AGTCCGGGGGGCATCCTGTTCCTGATTGCTGGGAAGATTCTGGAGATGGAAG2150.11542260779934718No Hit
GACCATGGGATGTCAGAGAAGTGCTATTTTTGAGGACAGATTGACAGATGTT2150.11542260779934718No Hit
ATTTCAGGGCAAATGACTAAGAAAGTTATAAAAGCCTCTCTACTCATCAAAG2140.11488575846074559No Hit
TACCACGGGGGTGCTGATAGTTTGTTAATGATTGAAATTTTAAAAAAATTAG2130.11434890912214396No Hit
GCATGTGGGTCCATGTACACAAAAAGTTATTTAACTCATCATGAATATTTAA2130.11434890912214396No Hit
ATAGCTGGTGAGGGCAGATTCCTATATAAGTGACTTTGTAACTTTAGAGACA2130.11434890912214396No Hit
GCTATGGGGAAGCTAGCGCCGTCGTCGTCTCCCTTCAGTCGCCATCATGATT2120.11381205978354234No Hit
GTTAATGGGGTATCCAGTGAAACTGAGCTTCATAAATGAAGGAAAAATACAG2110.11327521044494074No Hit
ACATCTGGGAACTATCAGCTGACTTATTAGAATAGAGACCATTAGATAGTGA2110.11327521044494074No Hit
AGTAATGGGCAGAAAAAAAGACGAGGATGCCTACTGTTGACACTTCTATTCA2110.11327521044494074No Hit
TGAACAGGGCATACAGTTTTTCTAGAAAGTTCATTGGTACTAGTAACGTATT2110.11327521044494074No Hit
TCCGATGGGATTCATCATATGGCTTTTTAGTTCTCATTAGATCTTTCTTCTG2110.11327521044494074No Hit
AGTCATGGGTTTCTTTATTTTAATCTTCAACTTTTGGTTTGATCAGAACCCA2100.11273836110633911No Hit
GACGCGGGCCTCATGAAAGGGACGACGGGAGCTGCATGAAAGCCGAAGTTAT2100.11273836110633911No Hit
CCAAGAGGGGAGGAATTAAGGAATAAGAACAGAGAGTCATATGAAGTATCTT2100.11273836110633911No Hit
CAGCTAGGGGCTGCAGCGAGTCTGGCGCAGAGTGGAGCGGCCGCCGGAGATG2090.1122015117677375No Hit
TCATTCGGGGTTTTCCTTCTTCCCGATTTCATGAATAGAAGACTCATTCTTA2090.1122015117677375No Hit
CTCAGAAGGGGCCGCTGTAAAATTTACCACAAACTTAGTGACTTAACACAAA2080.11166466242913589No Hit
GATCGTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2080.11166466242913589No Hit
TGCCGTGGGGGATCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACA2080.11166466242913589No Hit
AGTTATGGGGACTACACCTAGTTCATTAACTGTTTTTAGAAAGCACTTAATT2080.11166466242913589No Hit
ATTCATGGGATCGATGATGAGAATAATTGTCTGAGGATGCTGAGGGACTCAT2070.11112781309053428No Hit
AGACCTGGGCACTCTGAAAATTAATCATATTTTGGTAACACCAAAATTTACT2070.11112781309053428No Hit
GAAAGTGGGCGCCTAATATATAAAGTCAGAGAAATAAATGATAAAACAGATT2060.11059096375193266No Hit
TCAGCAAGGGCAATCCTATGGACAAGTTTACTCTTTCTAATCTAATTCTTGG2060.11059096375193266No Hit
TTATGCGGGAGGTGTTTAAGTTCCGCGCGCAGGCCAGGCTGCAACCTGACGG2060.11059096375193266No Hit
CGTACTGGGATTGCAGCCATCTGTGGGTTCACCTTCCAGACCCAGCAGCTCC2060.11059096375193266No Hit
TAACCAGGTAAATGTATGTCTTATTTTTTACAAAAACAGTTAAAAGTTAAAA2050.11005411441333103No Hit
TTTGCAGGGGAATTGGAAAAAACTACTTTAAAGTTCATATGGAACCAAAAAA2020.10844356639752621No Hit
CTTAGAGGGATGGAGATCCAGGTGAACGGAGGCACTGTGGCCGAGAAGCTGG2020.10844356639752621No Hit
TTCTATGGGTAATGAAAATTGCTTGTACTTGAAAAGTCGAGTCAGGAAGAAA2020.10844356639752621No Hit
AAGCCTGGGTGACAGAGCGAGACTGTGTCTTAAAGAAAAAGAAAAAAAGTCA2010.10790671705892459No Hit
GGCCATGATGCTTACAGGTTTTGTGAGATTGAGAGAACTATGACCTGCAGCA2000.10736986772032298No Hit
TAGGCGGGGGCTGTAGTTCGACCAATTTATGGATCGTGTCATGCCATGTAGA2000.10736986772032298No Hit
TCTCATGGGAACTTTCCTGGTGGGTAGGAGGCAAGCTGTGTTCTAAGATGAT1990.10683301838172135No Hit
ACAACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1990.10683301838172135No Hit
TACGCTGGGATCTCTATACAGGGACTAGGTAATAAAACCTTCAAAGGTTGTC1990.10683301838172135No Hit
GTTACTGGGGAATTCAGTCTTGCATGTGTTACGTTGGGGGACATTCGATGGA1990.10683301838172135No Hit
GTCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1990.10683301838172135No Hit
AGCCGTGGGTCCCGTGGGCACTGGCCAAGCTTTAGGGAGGCTCCTGGTCTGG1980.10629616904311973No Hit
CACACGGGACATAAATTTAAAGAAAATTTAATGCAAAGATAAGTTTTATGAA1980.10629616904311973No Hit
GCCTAAGGGTGGATCTTCCTTGTATTCTTCATGTCTCTTAGCTTTCTGATCG1980.10629616904311973No Hit
TTCTACGGGGCATAGAGGGTGTTTATAGGAAAGCGGTTGTATTACAAGCTCA1970.10575931970451813No Hit
ACATCTGGGCTAGTATAACTAATTCTGAGTAAACCAAAATGATAATAATTAA1970.10575931970451813No Hit
GCTAATGGGATATCCTACATGGTTAAAATGTAAGTTCTGCAGTTGAATGGAC1960.1052224703659165No Hit
TTCTTGGGGGGGGTTAGGGTAATCGATGTTATTGTTTAAAAGGTACAGAGTT1960.1052224703659165No Hit
TCATCAGGGAGAGCATCTTCATCACTCTTAGGAAACCAGGCATATCTCACCT1960.1052224703659165No Hit
TCTACAGGGTTGATTTTCCACAAATATTAGTAAACAGTTTTAGCCATCAGGA1960.1052224703659165No Hit
CTTAGGGGATGGAGGGTAGGAGAGCCTTGAGCCTGCGAGAAGAGTCTCTTAG1960.1052224703659165No Hit
ATTACTGGGGGAACCAAAAAAGAGCCTGCATTGCGAAGTCAATCCTAAGCCA1950.1046856210273149No Hit
TTAGATGGGCATTGCGGGTCCTGTGGTATAACCTTCTTGCCTTGACTAGAAG1940.10414877168871328No Hit
TCTGGGGTACATGCTATCAGCTTGATGGTGTATTAACTTTGGACACTTGGTT1930.10361192235011166No Hit
CAGCGGGATAGGAGAGTATACATTCTTCTCAAGCACACAAATAATTTACCAA1920.10307507301151005No Hit
GCCACGGGGGGAATTATTTAATTATTTAAATACAAAAACAACAATGTACTAT1920.10307507301151005No Hit
ATCCGAGGGGAAAGAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACC1910.10253822367290844No Hit
TTTGCCGGGATGGGTGGGAAGCTGTTGGATTAAATGTCACTATAATTACTGT1910.10253822367290844No Hit
TTGACAGGGATGTGAACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGT1910.10253822367290844No Hit
CAATATGGGGGTTGGGCTGGACTGCAGGTGCAGTTGGCAAAACTGTCCTGTC1910.10253822367290844No Hit
CTTGTTCGGGGTAGGCTCTCTGAGTTTTCATTTCTCATTGGGAATTGTAGAC1900.10200137433430682No Hit
TTGTGGGTAAAGACCAATTAACATTGACTGCTAAGGTGGACACTTATTCTTC1900.10200137433430682No Hit
GGTAGAGGGAGCTACAAAGATTAAGGCACCCTGGAAGCACAGAAGAGAAGTT1890.1014645249957052No Hit
GCTCGGGGTAATGGAGTTTACTCCAAGAAACTCAAAGCATTTCATACATTAT1890.1014645249957052No Hit
TCAACAGGGAGCTGCATTAGTCCCTAACAAGGGAGTCAGTCTGTCCTTTGAA1890.1014645249957052No Hit
GTCAATGGGATCCTAAGGAGAAAGAATAAACAATGAACCAATCAAAAATAAT1880.1009276756571036No Hit
CAGCAAGGGGGGGCGGGGGGCGCGCGCCGCTGCGGCACAGCCGGTCCCGGCT1880.1009276756571036No Hit
AGTTATGGGAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAATTAATACCA1870.10039082631850198No Hit
TCAGATGGGACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCT1870.10039082631850198No Hit
TAGTACGGGCAGGAAGGAAGCCCTTCCTTATGTTACATGTGGAAGGCCTGCC1870.10039082631850198No Hit
GCTTTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1870.10039082631850198No Hit
TAATACGGGTAGACTAAGGAAAAAACAGAGAAGACTCAAACTGCTAAAATCA1870.10039082631850198No Hit
GTGTTTGGGAACTGCTCCTGATACTTGTGTCTCCAGGGCACAGCCCACTTTC1870.10039082631850198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)