FastQCFastQC Report
Sat 31 Dec 2022
SRR5844036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187313
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC4360.2327654781034952No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT4350.2322316123280285RNA PCR Primer, Index 17 (95% over 22bp)
TGACTTGGGTATCTGCAGGAATTTATTTTTTTACGTATTTTTACTAGTTGAT3260.1740402428021547No Hit
CAACTAGGGAATAGAAAATGTTTAGAGTCAGGAGCACATTTCATAGTCCCCA3020.1612274641909531No Hit
GATGACGGGGGAGGAACATTGAGAAATTCACAAGTACATGGAAGTTAAATAA2890.15428720910988558No Hit
TTTCAGGGGCTAGAAAGCTATTAGGAAATCAAGGCATCTGGAGAAACTATGT2730.14574535670241787No Hit
GTATGGAGGGGCTGAATTCTATCAGGCATTCAAAGAAGAATTGGTACTAATT2720.14521149092695115No Hit
TGCTTGGGGATCTCTACTAAAAATACAAAAATTAGCTAGGCATGGTGGCACA2680.14307602782508422No Hit
GCGTAAGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2660.14200829627415076No Hit
AGCTGAGGGGCTGCTGGTTAACTTGAGTTTTAACACCTTTTTTTAAGTTCTC2650.14147443049868402No Hit
TGCAGAGGGATATAAAAACAAAACAACTGGTTCTGTGTGTATCAACTTAATT2610.1393389673968171No Hit
CAGTCTGGGGAAATGCCAAAACTTTCCAGGAAAAATAGTTTTATTATCAATC2530.13506804119308322No Hit
CATTCTGGGTGCTTTGTTTATGAAGCTGTGGGATACAAGGACTCTCATACGT2530.13506804119308322No Hit
GCACAAGGGGGCTTTGAACATGGGGTTGGCTGTTTCCCAGTAAAACTGGAAT2530.13506804119308322No Hit
GGCACAGGGTGCAAAATACAACTTCACTAGCAGTTTTGAGTTTAATCCTGAA2520.1345341754176165No Hit
CGCTCGGGGGTTGATGGAAGAGATGACCCATAGATGTCCCTACATCTCATTT2500.13346644386668305No Hit
GAAAGTGGGCGCCTAATATATAAAGTCAGAGAAATAAATGATAAAACAGATT2480.13239871231574957No Hit
TCAGCAGGGATGTCCGAAATTGCCTGACTTCATGTTTTGGAAGATTCCTAAG2470.13186484654028285No Hit
AAATGTGGGGGTAGAGGCTATGGCAAACTGGAGACTGAGTGCCTGTTCCTCA2450.13079711498934937No Hit
AAGTCAGGGGGAGCTGCCAGTTGTGGGAGGCAGCAGCAAACAAGTAGATAAA2440.13026324921388263No Hit
AGTTTAGGGCTGTATCAGGGATAATGGAAATGGAAGGATATAATTATAAATG2420.12919551766294918No Hit
ACATCTGGGAACTATCAGCTGACTTATTAGAATAGAGACCATTAGATAGTGA2420.12919551766294918No Hit
GACACTGGGCGATCTATGGTTGGGGTTCCTGTATATCAGTAGTACCAAAATT2410.12866165188748246No Hit
GCATGTGGGTCCATGTACACAAAAAGTTATTTAACTCATCATGAATATTTAA2410.12866165188748246No Hit
TACCTCGGGTGTTGATGCAGATGATGTGCGATTGGCAATCCAGTGCCGCGCT2410.12866165188748246No Hit
GACGCGGGCCTCATGAAAGGGACGACGGGAGCTGCATGAAAGCCGAAGTTAT2410.12866165188748246No Hit
AAGCATGGGTCGAACGGGGGGAGTGGCGTGGCGTGGCGGGGTCTCGCGGCGC2390.12759392033654898No Hit
TTATGCGGGAGGTGTTTAAGTTCCGCGCGCAGGCCAGGCTGCAACCTGACGG2390.12759392033654898No Hit
CCTTGCGGGGGCCGGCGCCAGGGCAGGCGGGCGGCTGGCAGCTGTGGCGCCG2390.12759392033654898No Hit
AGACCTGGGCACTCTGAAAATTAATCATATTTTGGTAACACCAAAATTTACT2380.12706005456108227No Hit
TGCACTGGGAGAACATAGGAAGGGTCATGTGAAAAGTCAGCATGTCTGGAAT2380.12706005456108227No Hit
AAGCCTGGGTGACAGAGCGAGACTGTGTCTTAAAGAAAAAGAAAAAAAGTCA2370.12652618878561553No Hit
TCCAATGGGGTTCTTTAATCATCATGATTCTGGTTTGTTGGATATCGGCAAA2350.12545845723468205No Hit
TCATTCGGGGTTTTCCTTCTTCCCGATTTCATGAATAGAAGACTCATTCTTA2350.12545845723468205No Hit
TGCCGTGGGGGATCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACA2350.12545845723468205No Hit
GCAACTGGGATATGGAGCCCAGATAAATGAACTTCATTTGGCATACTGCCTG2340.12492459145921533No Hit
GCTATGGGGAAGCTAGCGCCGTCGTCGTCTCCCTTCAGTCGCCATCATGATT2340.12492459145921533No Hit
CGTACCGGGATACTTAATGAGCAAATTGAAGTAAGCTTTTAAAGTATATTTC2330.1243907256837486No Hit
CTTAGTCAGGGATGAACAGACAATTCCCTAAGTTGTTTTGGTGCTTAGAATG2330.1243907256837486No Hit
AGTCATGGGTTTCTTTATTTTAATCTTCAACTTTTGGTTTGATCAGAACCCA2320.12385685990828185No Hit
GTTAATGGGGTATCCAGTGAAACTGAGCTTCATAAATGAAGGAAAAATACAG2310.12332299413281513No Hit
CTCAGAAGGGGCCGCTGTAAAATTTACCACAAACTTAGTGACTTAACACAAA2300.1227891283573484No Hit
TCCGCTGGGATAGTAGGAAGAAAGGCTCATTTTTTTTTATGATTTAGAAAGG2300.1227891283573484No Hit
AGTAATGGGCAGAAAAAAAGACGAGGATGCCTACTGTTGACACTTCTATTCA2290.12225526258188167No Hit
TATGCCGGGATTTCAAATTATTATTATGCAACCACAAGGATCTCTTCTTTCT2290.12225526258188167No Hit
TACGATGGGTACAGGAAATGGAAAAGCTTCCATGTGTTACCCTGTTTATGAA2290.12225526258188167No Hit
CTTGTTCGGGGTAGGCTCTCTGAGTTTTCATTTCTCATTGGGAATTGTAGAC2280.12172139680641492No Hit
ACATTAGGGATTTACAACTCAGTCATGGTGCTGCTGTCCTCCAGGCTTACTG2280.12172139680641492No Hit
GATCGTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2280.12172139680641492No Hit
GCAGTAGGGGAATAGGGCTTACTCACCCCTTCATGGAGGCTCGGGCAGCTAT2270.12118753103094819No Hit
TCTGGGGTACATGCTATCAGCTTGATGGTGTATTAACTTTGGACACTTGGTT2270.12118753103094819No Hit
TAAGCAGGGCGGGTTCCTCCAGAGAAGGTGGACCTTGGGGAGACAATCCATG2260.12065366525548146No Hit
GCCACAGGTGCTGAGCCGTCCCCGGCCCTTCTGGTGCCAAGTGCCAAGCGCC2260.12065366525548146No Hit
CGGCTTGGGTACAGAAAATGCACGTTTCCTGAAAACGTGGTGGGAACTCTTG2260.12065366525548146No Hit
AAAAGAGGGGAAGTGGAAGAAACGCGGAACCATGGCCGCTGTGGTTGCTGTT2240.119585933704548No Hit
AGTCCGGGGGGCATCCTGTTCCTGATTGCTGGGAAGATTCTGGAGATGGAAG2240.119585933704548No Hit
TGAAGCGGGAAGAGACACAGAGGAAATGATTTTGCCTTTAAGACACTCATGA2230.11905206792908127No Hit
TAGGCGGGGGCTGTAGTTCGACCAATTTATGGATCGTGTCATGCCATGTAGA2230.11905206792908127No Hit
TCCGATGGGATTCATCATATGGCTTTTTAGTTCTCATTAGATCTTTCTTCTG2220.11851820215361454No Hit
ATCCGAGGGGAAAGAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACC2210.11798433637814781No Hit
CACACGGGACATAAATTTAAAGAAAATTTAATGCAAAGATAAGTTTTATGAA2200.11745047060268109No Hit
TTTGCCGGGATGGGTGGGAAGCTGTTGGATTAAATGTCACTATAATTACTGT2200.11745047060268109No Hit
ACTGCAGGGGACACGTCCCCTGGGAATAATCTCCACTCTAGTTTGAGGATGA2200.11745047060268109No Hit
TGCTAGGGGCGATCTTTTCCCGGGACCTTTCAGAAATTTGTGTTCGAGTAAG2200.11745047060268109No Hit
AAGCCGGGGATTGGTGTTAAGGTGGTAATTTCCCACATATAAGATTTAGAAT2190.11691660482721433No Hit
CTAAGCGGGAAACAAAAACTTTCCATGACAGTAGCATACTGATGAGACAACA2190.11691660482721433No Hit
AACCTTGGGGGGATTATTATTTTTCAAAGTTGTAGAATCCAATAATACAGTC2190.11691660482721433No Hit
GTGTTGGGGACTATGGAAAATATATTGCGTACGGTCTTCTGATTTCTAGTTT2180.1163827390517476No Hit
GCTAATGGGATATCCTACATGGTTAAAATGTAAGTTCTGCAGTTGAATGGAC2150.11478114172534741No Hit
GGTAGAGGGAGCTACAAAGATTAAGGCACCCTGGAAGCACAGAAGAGAAGTT2150.11478114172534741No Hit
TATATGGGTCTTCTAGTGGGGGCTGAGCTCTACGAGGCGGAGCGGCGGCGGT2150.11478114172534741No Hit
ATGTTGGGGCAAGACCATGGGAACCCACCTCTTGCATCAGCATGACCTGGAT2140.11424727594988068No Hit
CCTGGCCTTGTGATCCGCCAGCCTCGGCCTCCCAAGGTGCTGGGATTGCAGG2130.11371341017441396No Hit
GCTCGGGGTAATGGAGTTTACTCCAAGAAACTCAAAGCATTTCATACATTAT2120.11317954439894723No Hit
ATTACTGGGGGAACCAAAAAAGAGCCTGCATTGCGAAGTCAATCCTAAGCCA2110.11264567862348047No Hit
ATAGCTGGTGAGGGCAGATTCCTATATAAGTGACTTTGTAACTTTAGAGACA2110.11264567862348047No Hit
TTTGCAGGGGGGTGTTAGTTCCCGGTCACCTGAGCTCCGGGTGACGCGGCTG2110.11264567862348047No Hit
TAACCAGGTAAATGTATGTCTTATTTTTTACAAAAACAGTTAAAAGTTAAAA2100.11211181284801375No Hit
AAAAGCAGGGGGTGCTCAGACAGCCTTAAACAAAGGTGGCTGAGAGTGAGGA2080.1110440812970803No Hit
GGCTACGGGAATGAGCCTATTGGGTGGATTCAAAACTGATAAAATATTTGGA2070.11051021552161355No Hit
TACGCTGGGATCTCTATACAGGGACTAGGTAATAAAACCTTCAAAGGTTGTC2070.11051021552161355No Hit
CAGCGGGATAGGAGAGTATACATTCTTCTCAAGCACACAAATAATTTACCAA2060.10997634974614683No Hit
ATTTCAGGGCAAATGACTAAGAAAGTTATAAAAGCCTCTCTACTCATCAAAG2060.10997634974614683No Hit
TCTACAGGGTTGATTTTCCACAAATATTAGTAAACAGTTTTAGCCATCAGGA2050.1094424839706801No Hit
TCAGCAAGGGCAATCCTATGGACAAGTTTACTCTTTCTAATCTAATTCTTGG2040.10890861819521337No Hit
GTCAATGGGATCCTAAGGAGAAAGAATAAACAATGAACCAATCAAAAATAAT2040.10890861819521337No Hit
ATAAAGGGGGCCACTGGGCCATGGAGACTGTGGCACAGTAGACTGTAGTGTG2030.10837475241974662No Hit
GCCACGGGGGGAATTATTTAATTATTTAAATACAAAAACAACAATGTACTAT2030.10837475241974662No Hit
TGAACAGGGCATACAGTTTTTCTAGAAAGTTCATTGGTACTAGTAACGTATT2020.10784088664427989No Hit
AATACCGGGATTGGGCGGTGTCTTGGTGGCATGCTTCAGGGCTCCCCTGTGT2020.10784088664427989No Hit
ACATCTGGGCTAGTATAACTAATTCTGAGTAAACCAAAATGATAATAATTAA2020.10784088664427989No Hit
ATTCATGGGATCGATGATGAGAATAATTGTCTGAGGATGCTGAGGGACTCAT2010.10730702086881316No Hit
TAGTACGGGCAGGAAGGAAGCCCTTCCTTATGTTACATGTGGAAGGCCTGCC2010.10730702086881316No Hit
TACCACGGGGGTGCTGATAGTTTGTTAATGATTGAAATTTTAAAAAAATTAG2000.10677315509334644No Hit
ACAGCTGGGGTTTTGGACATGTCTAAGAACCCAGGTATAGAATAATAAAGTT2000.10677315509334644No Hit
TCTCATGGGAACTTTCCTGGTGGGTAGGAGGCAAGCTGTGTTCTAAGATGAT2000.10677315509334644No Hit
TTGTGTAGGGTTAATGAAAATTGGACAGTATGTATAATCCAGGGCTAAATAG1990.10623928931787971No Hit
AGTTATGGGGACTACACCTAGTTCATTAACTGTTTTTAGAAAGCACTTAATT1990.10623928931787971No Hit
GAATCTGGGATAGTGAAAATTGAAACTAGACTCCTTCCTTTCACCATACACA1970.10517155776694623No Hit
GCTCCGGGGAAGATTATTGGATAGCCTTTAAAAAACCTGCACCCATTTCATG1960.1046376919914795No Hit
TTTGCAGGGGAATTGGAAAAAACTACTTTAAAGTTCATATGGAACCAAAAAA1950.10410382621601277No Hit
CTTCAGGGGGGGGTCAGGGCTTCAACATATGAATTTGGAGAGAAAACACAGT1950.10410382621601277No Hit
TTCTACGGGGCATAGAGGGTGTTTATAGGAAAGCGGTTGTATTACAAGCTCA1950.10410382621601277No Hit
TTTTGCGGGTGCTCCTCTGCGAGCATCTCTGGTGCCCATGGAACACTGCATA1940.10356996044054603No Hit
CAGCAAGGGGGGGCGGGGGGCGCGCGCCGCTGCGGCACAGCCGGTCCCGGCT1940.10356996044054603No Hit
TCGAGAGGGAGGCATGGTGGTGAGGACTACGTCTTCTCCCTGCTCACGGGCT1930.1030360946650793No Hit
TAGCCTGGGAGAATTTGAGTGATCTATGCTGAATAAACAGTGGAATGTGACC1930.1030360946650793No Hit
AGCTAAGGAAAGGCAAAAATGAAACTAAATGAAAGCGAAAGGGAAAATAAAG1920.10250222888961258No Hit
GTGGCGGGGGTTTTTTTTAATGTGTGTTTATATCTACCAGAGTCTTCAGAGA1920.10250222888961258No Hit
GCATTGGGGACTTTAGGGACTTCCACAATTATGTTCTGATCAATCCTAGGTT1920.10250222888961258No Hit
TAATTGGGAGAGGATGTGGAGCAATAGGAACACTTTTACACTGTTGGTGGGA1920.10250222888961258No Hit
GTCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1910.10196836311414585No Hit
TTCTTGGGGGGGGTTAGGGTAATCGATGTTATTGTTTAAAAGGTACAGAGTT1900.1014344973386791No Hit
TTCTATGGGTAATGAAAATTGCTTGTACTTGAAAAGTCGAGTCAGGAAGAAA1900.1014344973386791No Hit
TTCAGCGGGGATTGCAGAATCCCTTCCTGGAGCCCTGAAGTTCCTGGAAAAC1890.10090063156321237No Hit
TCTACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1890.10090063156321237No Hit
GACCATGGGATGTCAGAGAAGTGCTATTTTTGAGGACAGATTGACAGATGTT1890.10090063156321237No Hit
TCAGATGGGACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCT1880.10036676578774564No Hit
TCAGCTGGGAATGAACCCCCGAGGCCCAAATGAAATAAGGCAGGATTTGATG1880.10036676578774564No Hit
GCTGTGTAGGGCTCTTTCTAACTTTTAGTAATTGTCAATAACACTTATATTA1880.10036676578774564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)