FastQCFastQC Report
Sat 31 Dec 2022
SRR5844035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186414
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC4250.2279871683457251No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT4060.21779480081968094RNA PCR Primer, Index 17 (95% over 22bp)
TGACTTGGGTATCTGCAGGAATTTATTTTTTTACGTATTTTTACTAGTTGAT2860.15342195328677033No Hit
CAACTAGGGAATAGAAAATGTTTAGAGTCAGGAGCACATTTCATAGTCCCCA2830.15181263209844753No Hit
CGGCTTGGGTACAGAAAATGCACGTTTCCTGAAAACGTGGTGGGAACTCTTG2780.14913043011790958No Hit
GATGACGGGGGAGGAACATTGAGAAATTCACAAGTACATGGAAGTTAAATAA2740.14698466853347925No Hit
AAGTCAGGGGGAGCTGCCAGTTGTGGGAGGCAGCAGCAAACAAGTAGATAAA2730.14644822813737166No Hit
TCAGCAGGGATGTCCGAAATTGCCTGACTTCATGTTTTGGAAGATTCCTAAG2680.14376602615683373No Hit
GTATGGAGGGGCTGAATTCTATCAGGCATTCAAAGAAGAATTGGTACTAATT2660.14269314536461852No Hit
ACATTAGGGATTTACAACTCAGTCATGGTGCTGCTGTCCTCCAGGCTTACTG2630.14108382417629578No Hit
AGTTTAGGGCTGTATCAGGGATAATGGAAATGGAAGGATATAATTATAAATG2570.13786518179965024No Hit
TGCAGAGGGATATAAAAACAAAACAACTGGTTCTGTGTGTATCAACTTAATT2560.13732874140354265No Hit
TCATTCGGGGTTTTCCTTCTTCCCGATTTCATGAATAGAAGACTCATTCTTA2560.13732874140354265No Hit
GCGTAAGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.13679230100743506No Hit
TTTCAGGGGCTAGAAAGCTATTAGGAAATCAAGGCATCTGGAGAAACTATGT2540.1362558606113275No Hit
TGAAGCGGGAAGAGACACAGAGGAAATGATTTTGCCTTTAAGACACTCATGA2520.1351829798191123No Hit
CGCTCGGGGGTTGATGGAAGAGATGACCCATAGATGTCCCTACATCTCATTT2510.1346465394230047No Hit
AACCTTGGGGGGATTATTATTTTTCAAAGTTGTAGAATCCAATAATACAGTC2460.13196433744246677No Hit
AGCTGAGGGGCTGCTGGTTAACTTGAGTTTTAACACCTTTTTTTAAGTTCTC2450.13142789704635918No Hit
GAAAGTGGGCGCCTAATATATAAAGTCAGAGAAATAAATGATAAAACAGATT2430.130355016254144No Hit
TGAACAGGGCATACAGTTTTTCTAGAAAGTTCATTGGTACTAGTAACGTATT2430.130355016254144No Hit
AGTAATGGGCAGAAAAAAAGACGAGGATGCCTACTGTTGACACTTCTATTCA2420.1298185758580364No Hit
GACGCGGGCCTCATGAAAGGGACGACGGGAGCTGCATGAAAGCCGAAGTTAT2420.1298185758580364No Hit
CATTCTGGGTGCTTTGTTTATGAAGCTGTGGGATACAAGGACTCTCATACGT2410.12928213546192885No Hit
TGCTTGGGGATCTCTACTAAAAATACAAAAATTAGCTAGGCATGGTGGCACA2400.12874569506582123No Hit
CTAAGCGGGAAACAAAAACTTTCCATGACAGTAGCATACTGATGAGACAACA2380.12767281427360605No Hit
GCACAAGGGGGCTTTGAACATGGGGTTGGCTGTTTCCCAGTAAAACTGGAAT2360.1265999334813909No Hit
AAATGTGGGGGTAGAGGCTATGGCAAACTGGAGACTGAGTGCCTGTTCCTCA2350.1260634930852833No Hit
TCCGCTGGGATAGTAGGAAGAAAGGCTCATTTTTTTTTATGATTTAGAAAGG2350.1260634930852833No Hit
GACACTGGGCGATCTATGGTTGGGGTTCCTGTATATCAGTAGTACCAAAATT2330.12499061229306811No Hit
AAAAGAGGGGAAGTGGAAGAAACGCGGAACCATGGCCGCTGTGGTTGCTGTT2330.12499061229306811No Hit
TTGTGTAGGGTTAATGAAAATTGGACAGTATGTATAATCCAGGGCTAAATAG2330.12499061229306811No Hit
TCCAATGGGGTTCTTTAATCATCATGATTCTGGTTTGTTGGATATCGGCAAA2320.12445417189696052No Hit
GGCACAGGGTGCAAAATACAACTTCACTAGCAGTTTTGAGTTTAATCCTGAA2320.12445417189696052No Hit
AAGCCGGGGATTGGTGTTAAGGTGGTAATTTCCCACATATAAGATTTAGAAT2310.12391773150085295No Hit
GCAGTAGGGGAATAGGGCTTACTCACCCCTTCATGGAGGCTCGGGCAGCTAT2310.12391773150085295No Hit
TGCTAGGGGCGATCTTTTCCCGGGACCTTTCAGAAATTTGTGTTCGAGTAAG2310.12391773150085295No Hit
ATTCATGGGATCGATGATGAGAATAATTGTCTGAGGATGCTGAGGGACTCAT2280.12230841031253019No Hit
CAGCAAGGGGGGGCGGGGGGCGCGCGCCGCTGCGGCACAGCCGGTCCCGGCT2260.121235529520315No Hit
TAGGCGGGGGCTGTAGTTCGACCAATTTATGGATCGTGTCATGCCATGTAGA2260.121235529520315No Hit
TACGATGGGTACAGGAAATGGAAAAGCTTCCATGTGTTACCCTGTTTATGAA2260.121235529520315No Hit
GCCACAGGTGCTGAGCCGTCCCCGGCCCTTCTGGTGCCAAGTGCCAAGCGCC2250.1206990891242074No Hit
TGCCGTGGGGGATCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACA2250.1206990891242074No Hit
TGCACTGGGAGAACATAGGAAGGGTCATGTGAAAAGTCAGCATGTCTGGAAT2250.1206990891242074No Hit
GCTATGGGGAAGCTAGCGCCGTCGTCGTCTCCCTTCAGTCGCCATCATGATT2240.12016264872809983No Hit
AGACCTGGGCACTCTGAAAATTAATCATATTTTGGTAACACCAAAATTTACT2230.11962620833199224No Hit
GCATGTGGGTCCATGTACACAAAAAGTTATTTAACTCATCATGAATATTTAA2210.11855332753977704No Hit
AGCCGTGGGTCCCGTGGGCACTGGCCAAGCTTTAGGGAGGCTCCTGGTCTGG2200.11801688714366947No Hit
GTTAATGGGGTATCCAGTGAAACTGAGCTTCATAAATGAAGGAAAAATACAG2190.11748044674756188No Hit
CTCAGAAGGGGCCGCTGTAAAATTTACCACAAACTTAGTGACTTAACACAAA2190.11748044674756188No Hit
TCCGATGGGATTCATCATATGGCTTTTTAGTTCTCATTAGATCTTTCTTCTG2190.11748044674756188No Hit
CACACGGGACATAAATTTAAAGAAAATTTAATGCAAAGATAAGTTTTATGAA2180.11694400635145429No Hit
CCTTGCGGGGGCCGGCGCCAGGGCAGGCGGGCGGCTGGCAGCTGTGGCGCCG2160.11587112555923912No Hit
ATCCGAGGGGAAAGAGGGAATGCCCCAACTAAATACTACCGTATGGCCCACC2150.11533468516313151No Hit
GCCAATGGGATCTTATTTTCCCATCAGATTATAGAATGCCTAAGGTCTAGTG2150.11533468516313151No Hit
GCTGTGTAGGGCTCTTTCTAACTTTTAGTAATTGTCAATAACACTTATATTA2140.11479824476702392No Hit
TCAGATGGGACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCT2130.11426180437091635No Hit
CAGTCTGGGGAAATGCCAAAACTTTCCAGGAAAAATAGTTTTATTATCAATC2130.11426180437091635No Hit
TTCTTGGGGGGGGTTAGGGTAATCGATGTTATTGTTTAAAAGGTACAGAGTT2130.11426180437091635No Hit
CCAAGAGGGGAGGAATTAAGGAATAAGAACAGAGAGTCATATGAAGTATCTT2130.11426180437091635No Hit
GTGTTGGGGACTATGGAAAATATATTGCGTACGGTCTTCTGATTTCTAGTTT2120.11372536397480876No Hit
AAGCATGGGTCGAACGGGGGGAGTGGCGTGGCGTGGCGGGGTCTCGCGGCGC2110.11318892357870117No Hit
TACCTCGGGTGTTGATGCAGATGATGTGCGATTGGCAATCCAGTGCCGCGCT2110.11318892357870117No Hit
CTTAGTCAGGGATGAACAGACAATTCCCTAAGTTGTTTTGGTGCTTAGAATG2110.11318892357870117No Hit
TTAGATGGGCATTGCGGGTCCTGTGGTATAACCTTCTTGCCTTGACTAGAAG2100.11265248318259359No Hit
ACATCTGGGAACTATCAGCTGACTTATTAGAATAGAGACCATTAGATAGTGA2090.112116042786486No Hit
ACACCTGGGAGAAGAATGGATAAAGGATGTGATGAGGAGATGGTTTACATAA2090.112116042786486No Hit
GGCTACGGGAATGAGCCTATTGGGTGGATTCAAAACTGATAAAATATTTGGA2070.1110431619942708No Hit
TACGCTGGGATCTCTATACAGGGACTAGGTAATAAAACCTTCAAAGGTTGTC2070.1110431619942708No Hit
ATGGCGGGGGAATCAACTCCTGTGTCACCTACTTTGCCAGTACGATCTGTTC2070.1110431619942708No Hit
AGTTATGGGGACTACACCTAGTTCATTAACTGTTTTTAGAAAGCACTTAATT2070.1110431619942708No Hit
TATGCCGGGATTTCAAATTATTATTATGCAACCACAAGGATCTCTTCTTTCT2060.11050672159816323No Hit
ATAAAGGGGGCCACTGGGCCATGGAGACTGTGGCACAGTAGACTGTAGTGTG2050.10997028120205564No Hit
GCAACTGGGATATGGAGCCCAGATAAATGAACTTCATTTGGCATACTGCCTG2050.10997028120205564No Hit
TCAGCTGGGAATGAACCCCCGAGGCCCAAATGAAATAAGGCAGGATTTGATG2050.10997028120205564No Hit
CGTACTGGGATTGCAGCCATCTGTGGGTTCACCTTCCAGACCCAGCAGCTCC2050.10997028120205564No Hit
CGTACCGGGATACTTAATGAGCAAATTGAAGTAAGCTTTTAAAGTATATTTC2040.10943384080594806No Hit
TAGCCTGGGAGAATTTGAGTGATCTATGCTGAATAAACAGTGGAATGTGACC2040.10943384080594806No Hit
TTTGCAGGGGAATTGGAAAAAACTACTTTAAAGTTCATATGGAACCAAAAAA2030.10889740040984047No Hit
AGGCATGGGATGGGGGTTGGGGGGTTATTTATTTTGCCTGTCCTTATCCCTG2030.10889740040984047No Hit
TAATTGGGAGAGGATGTGGAGCAATAGGAACACTTTTACACTGTTGGTGGGA2030.10889740040984047No Hit
CAATATGGGGGTTGGGCTGGACTGCAGGTGCAGTTGGCAAAACTGTCCTGTC2030.10889740040984047No Hit
AGTCCGGGGGGCATCCTGTTCCTGATTGCTGGGAAGATTCTGGAGATGGAAG2030.10889740040984047No Hit
ACTTTGGGGAAATCCTGCACTGAAACAACAGGAAAATGGTCAAGAGACAGGA2020.10836096001373287No Hit
TCTACAGGGTTGATTTTCCACAAATATTAGTAAACAGTTTTAGCCATCAGGA2010.10782451961762528No Hit
CCTGGCCTTGTGATCCGCCAGCCTCGGCCTCCCAAGGTGCTGGGATTGCAGG2000.10728807922151769No Hit
ATTTATGGGAGATGCGCCACGGTTTCGGTAGCGACGGTATCTCTAGCCGGGC2000.10728807922151769No Hit
GTTACTGGGGAATTCAGTCTTGCATGTGTTACGTTGGGGGACATTCGATGGA2000.10728807922151769No Hit
TTTGCCGGGATGGGTGGGAAGCTGTTGGATTAAATGTCACTATAATTACTGT1980.10621519842930252No Hit
CCACTAGGGATAAGAGTTTTGTTAAGATTTCTTAGTACTTTAGACCTTAAAT1980.10621519842930252No Hit
GCATAAGGGGGTTCGGACTAACATTTCACATGCACATTTCAAAACAAGATGT1980.10621519842930252No Hit
CAACAGGGGATTTCAACGTGAGTTTTGATGAGGACAAACCACATCCAAATCA1980.10621519842930252No Hit
TTCTATGGGTAATGAAAATTGCTTGTACTTGAAAAGTCGAGTCAGGAAGAAA1980.10621519842930252No Hit
TTTGCAGGGGGGTGTTAGTTCCCGGTCACCTGAGCTCCGGGTGACGCGGCTG1980.10621519842930252No Hit
TAAGCAGGGCGGGTTCCTCCAGAGAAGGTGGACCTTGGGGAGACAATCCATG1970.10567875803319494No Hit
GCTCCATTGTGCAAATTTTTTTTATTATTGCATTTTATTTTGACCCTGGACT1970.10567875803319494No Hit
TCGAGAGGGAGGCATGGTGGTGAGGACTACGTCTTCTCCCTGCTCACGGGCT1970.10567875803319494No Hit
AGCTAAGGAAAGGCAAAAATGAAACTAAATGAAAGCGAAAGGGAAAATAAAG1960.10514231763708735No Hit
AATTGTGGGCCGATCTTGAAGAGGGGATCACACTGATAGAGATGAGGAGCAG1960.10514231763708735No Hit
GTCAGGGGGGGGGCCAGCGAGGCAAGATGGAGTTAGTGCAGGTCCTGAAACG1960.10514231763708735No Hit
TTGTTGGGGAATGACTTCAAGGTTCATCCATGTTGTAAACATGTATCAGAAT1960.10514231763708735No Hit
TATCGCGGGTTATAGTTTAAATGTGTATGTGATTCTTTTTGATGTTTGTCGA1960.10514231763708735No Hit
ATTTCAGGGCAAATGACTAAGAAAGTTATAAAAGCCTCTCTACTCATCAAAG1950.10460587724097975No Hit
TCTCATGGGAACTTTCCTGGTGGGTAGGAGGCAAGCTGTGTTCTAAGATGAT1950.10460587724097975No Hit
TTTGCTGGGAACTATAATTTTATTAGAAGGCTTAATACGTGTTTCTAAACTT1940.10406943684487216No Hit
CGCCACTGCACTCCAACCTGGGCAACAGAGCGAGACTCCATCCCCCCCCAAA1930.10353299644876457No Hit
ATCTAAGGGATGAGGAACTGACCAATGATAGCCTGACCCTATCTCAAAGCAA1930.10353299644876457No Hit
TAACCAGGTAAATGTATGTCTTATTTTTTACAAAAACAGTTAAAAGTTAAAA1930.10353299644876457No Hit
AAGCCTGGGTGACAGAGCGAGACTGTGTCTTAAAGAAAAAGAAAAAAAGTCA1930.10353299644876457No Hit
TCTGGGGTACATGCTATCAGCTTGATGGTGTATTAACTTTGGACACTTGGTT1920.10299655605265699No Hit
TCATCAGGGAGAGCATCTTCATCACTCTTAGGAAACCAGGCATATCTCACCT1910.1024601156565494No Hit
GCCTAAGGGTGGATCTTCCTTGTATTCTTCATGTCTCTTAGCTTTCTGATCG1910.1024601156565494No Hit
GCACCGGGGTGACATTGGCTGTTTCTAGAGGGTGCTAAGACATGGGGCCAGG1910.1024601156565494No Hit
CATTTAGGGGACTACCAACAGAAGAGAAGAGAAGCCATGAATAAGCAACCAG1900.10192367526044183No Hit
TAACGCTGGGATTTGTAGGTGGGGTTCATTCTATATTTACTGTGTTGAATGT1900.10192367526044183No Hit
AGTCATGGGTTTCTTTATTTTAATCTTCAACTTTTGGTTTGATCAGAACCCA1890.10138723486433421No Hit
ACTGCAGGGGACACGTCCCCTGGGAATAATCTCCACTCTAGTTTGAGGATGA1890.10138723486433421No Hit
TACTACAGGGGATCATGAGGATTAAACAGAGTAAATATAAATTATCTAGTAT1890.10138723486433421No Hit
TTATGCGGGAGGTGTTTAAGTTCCGCGCGCAGGCCAGGCTGCAACCTGACGG1880.10085079446822663No Hit
TTTTAAGGGCTCAGTTGCAACAACCAAAACAACTATAGACATTGCTAAGTGT1880.10085079446822663No Hit
GCTCGGGGTAATGGAGTTTACTCCAAGAAACTCAAAGCATTTCATACATTAT1880.10085079446822663No Hit
CTCTCAGGGAGAATAGGCACTGAATATGTATTTCATCACAGACTCTCTTAGG1880.10085079446822663No Hit
GATTTGGGGGGAAATGTGTCCCACTTTCACAGACCAGCTCTAAAATCTCAAA1880.10085079446822663No Hit
TAAGTAGGGGCAGACTTGTTATTGTAGTTGCTTCATAGTGTCATTGCTTTGT1870.10031435407211904No Hit
TAGTAGGGGTATAGAGTCAATATTAATTTTTTTGACTTTGATAATGATATTG1870.10031435407211904No Hit
TTGTAAGGGAAGGAGAAAGATAAAAAAAGTCTCCAAAATTATACTCACCAGA1870.10031435407211904No Hit
GTGGCGGGGGTTTTTTTTAATGTGTGTTTATATCTACCAGAGTCTTCAGAGA1870.10031435407211904No Hit
TTGTGTGGGCTAGCAGGGAGCCAGCTAGAGAGTGATTATTGAAATGTGAAAA1870.10031435407211904No Hit
AGTCATGGGGTGCCTATTTCAAAAACTAGGCAATTTAAGATTATTCAGTCAG1870.10031435407211904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)