FastQCFastQC Report
Sat 31 Dec 2022
SRR5844028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58764
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC2000.34034442856170444No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT1840.3131168742767681RNA PCR Primer, Index 17 (95% over 22bp)
CTTGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA920.15655843713838405No Hit
GCTTGGGTATACTTGGACCCCATGGCTGTGGTGGCAGAAGGAGTGCCCTGGT850.14464638213872438No Hit
TTTTGAGGGCATTTCCAAGGTGCAAGTGTTTATTTTTCAAATCTATAAGTAC850.14464638213872438No Hit
CATGGTGGGGCTACTCTTTTGGCTAAGACATCTTTGGTTTCTGCCATTTGCC840.14294465999591588No Hit
TGAATAGGGGTTAGCATCAGTGACCTCCTGGTACCAAAAGACTTGGGAACAG830.14124293785310735No Hit
CCGAACGGGAGTCTGGGACGCGCCGCCGCCATGATCATCCCTGTACGCTGCT830.14124293785310735No Hit
CTATATGGGTGAGGGGTCTTTAGTAATGCATCTGGGTAGCATCTCAACTGCT830.14124293785310735No Hit
TGACAAGGGAAGCGGAAAATAATGATCAAGCGGCACGAGGTGGAGCAGCAGA830.14124293785310735No Hit
AGCAAGGGATTCAGAACCTGTTAGATTAAAGCTGCACCCTGTGATTATTTGA800.13613777142468178No Hit
TCAGCCAGCAGCCCCAAGCCCGACACCAAGGTGCCCCAGGTCACCACCGAGG790.13443604928187325No Hit
CAAATGGGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGT790.13443604928187325No Hit
TAAAAGCGGGAGCACGGTTCCTGAGACATAGTAGCCACTCAATAAATGTTAG790.13443604928187325No Hit
ATAGCTGGGGGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTG780.13273432713906472No Hit
ATCAGAGGGAACTTTGGGAATGAATAAAGTGGAATGGTAACTTTCCAGTGGT760.12933088285344768No Hit
AACGGGGCCGTGAAAGTTGGAAGTGTTGATTGCCTTCAGGAAGAGATTGATG720.12252399428221361No Hit
GTCATGGGGAATCAAGAGTTAACAAACCTAACGTAACCTGAGGTTTAATATT720.12252399428221361No Hit
ACAGTAGGGATGAAATTATGAATTTGAAGCCTCTGAAATGGTGATCAGTTTT720.12252399428221361No Hit
ACCTAAGGGTCTGTTTGTTAGGATTTGAAATTCTGGAAAATATTTATCTACA720.12252399428221361No Hit
GATGCTGGGCTTTGAAAGCAATTAGGTTATTCACCTGGTATTAAAACTATTT720.12252399428221361No Hit
CAGTATGGGAGTGAGACTGACTGCGTCGGGGTTGAGACTGGGTGGATGAGGC720.12252399428221361No Hit
GCACTGGGGTCTCAAGAAGGGGGAGGCTAAAAACCTGAAGAAGATGGACGTA720.12252399428221361No Hit
AGTGCGGGATTCTCTAGGAACTCACTTTTGAGAGTATTTTTCTCTAACCCAT710.12082227213940508No Hit
GAACCGGGACATGGCACTGAGTGTACTCTCTGCCAGTGCAAGAATGGCCACA710.12082227213940508No Hit
GCAACTGGGCTTCAAAGGATACCATCACATTTTATAATTCATAGATCTGATA710.12082227213940508No Hit
AAGTTAGGGAGAATGAGGAATGAGGGAAGAAGAGAAATTAAGGGTGTCTCCT700.11912054999659656No Hit
ATAATAGGGCATCACTGGTCATTAGAGAAATGCAAATCAAAACCACAATGAG700.11912054999659656No Hit
GGCATAGGGATAATGAATAAATGTTCTTGCTTTGTATGGAAATACAATTCTT680.11571710571097953No Hit
GTTCTCGGGACCACGCCGAGCTCACCGTGCCCGTCCTGCTTGTCCACGGCAT680.11571710571097953No Hit
TAAGTTGGGGGAGGAAGAGGGCGGCGACGGTGGTGGTGACTGAGCGGAGCCC680.11571710571097953No Hit
GGCTAGGGGGTTTTGCAGACGCCACCGCCGAGGAAAACCGTGTACTATTAGC680.11571710571097953No Hit
TGTATTGGGATAGGGATGTTGGTATCTACTGTGGGTTGGGTAGGGCCGATGT680.11571710571097953No Hit
ACACATGGGCAGCCTGGGCATACTTTCCCATCAGAGTCAGCCTCTATCACAA680.11571710571097953No Hit
CGTGACGGGCGTTGAGAGAGAGCCTTGGGACGGCAGGGATGGCGAGCTTCCC670.114015383568171No Hit
AACCACGGGGCTGTTTTTGATGGACATGGAGGAATTCGAGCCTCAAAATTTG670.114015383568171No Hit
TTCCTAGGGGGCTTCCTTCTGCAACAGTCGTGGGTCACGCTCTCGCTCGGTC670.114015383568171No Hit
GATGCAGGGAGCGCGTTTACCACAAAGCTGTGCATATAGGTGGCACCATATA670.114015383568171No Hit
TAGCTAGGGAATGCTCAGGGAGAGTGATTTGAGTAATAATGAAACTGTTGTC670.114015383568171No Hit
TAGAAAGGGTATATCATTTATATGTTTTAATAGATTTCAACGAAAAGCTCCA670.114015383568171No Hit
CAAACTGGGAGGGTTAAAAATGCTGAGTGAATACTGCAAATATACCTTAGCA670.114015383568171No Hit
GAAGCTGGGGTTAAGATAATTATATTGCCACTAATAATCAAGATGCTAAATG670.114015383568171No Hit
ATCGCTGGGGTTGCAAATTTGTTAAAATGCCAAAAAGGACAGTTTAATTTTG670.114015383568171No Hit
ACTGGTGGGATTTCAGTTAAAAGGCAGAGATTATCAGATTGGCTAGAAAAGA670.114015383568171No Hit
CCGCGTGGGATGATTATTTTTATAATCAGAAAAGTTATAAACAGTCCCACTG660.11231366142536246No Hit
TGACGGGGGGCTACAACCCTGGGCAAGTTACTTCACCTCTGAGCCTCCGATG660.11231366142536246No Hit
ACCGTGGGCCACGGAGTCCCTCCCAGGACACATGGGGATTACGGGAACTACA660.11231366142536246No Hit
TTCGCGGGGGGGCCCTTATTTGCTGTTCTAAGAGTTGTCTGTAGGGGTTTTT660.11231366142536246No Hit
TGTGTAGGGGGAAATTTTCCCAGCTTAGCATGAACATGGATCATACAGTGTT660.11231366142536246No Hit
CGCTATGGGTAAATAAATAAACTAAATCAATCAATCCATCAATCAGTCGACC650.11061193928255394No Hit
GTTTAAGGGATACTTCGGCAAACTTATTGGGTCTTCATTTTCTGACAGACAG650.11061193928255394No Hit
AGCTTTGGGCCAGTCCAAGAACCTGCCTAAAGCCGACAGACAGGCCCTGAAT650.11061193928255394No Hit
GCGTACGGGATGCCCAGCCAGCCACCCCTGCCACTCACCCCTCCTGGCCCAG650.11061193928255394No Hit
CTACGAGGGATTTGTTCAGACACTGAATCTTAATTTTACCATTTCCCTTTTT650.11061193928255394No Hit
AGTTTGGGGGGAAAATATCACAGTCCTAAATATATATGCACCTAATACTGGA650.11061193928255394No Hit
GCACTGGGGTTGAATATGATTTGGTCAGTTGCTCGTTGTAACTTGGAGAAAT650.11061193928255394No Hit
CTACCGGGGAATCGCCGAGGGCACGTGCATGCCCCCTGGTTAAGAGTTGCAG650.11061193928255394No Hit
AGAGTGGGGCAATGAGACAGCTAAAAGTTAGTATGTGGGGGGATGGAGTTAA650.11061193928255394No Hit
CTACCCGGGTAAAATACAGCCCGAACTGCAAGTTGCTTATTGTTTCAAATCC640.10891021713974541No Hit
TTACAAGGGATATGAGAAAGTAGAGAGGGAAATGGGGAATGATTGGTACAAA640.10891021713974541No Hit
GTTGACGGGGAAATGACTAACACTAGCTAATTAACATATGCATTACCTCAAA640.10891021713974541No Hit
GTCGCAGGGACTTTGGGAAGTTATTTCTTTATTTTAATGATGTATCATTGTT640.10891021713974541No Hit
GTACGTGGGGTTTATTGTTTAATTTATGCCCAAGTGAAGTTGTAAACTTATG640.10891021713974541No Hit
TCAGCGGGGAGACATCTCACTACTACTACTGACTATCACACACTGGTTGCCA640.10891021713974541No Hit
GCATATGGGCCAAGCCGCGCTACATGCAGTGGCGGGAAGGCATCAGCTCCAG640.10891021713974541No Hit
CATCAGGGGCCGTTGAAAGGCGCTTCACAGTGCTTCAACCTGTAGCTTCAGC640.10891021713974541No Hit
TGCAGCGGGGTGCTGTAAGTGCCATTTCTCAGTATTTTCAAGGCTCTAACCC640.10891021713974541No Hit
TATCTTGGGATCTCTCAACTCTCAGCACAGTGCTACTGCGAAACTGATGGGA640.10891021713974541No Hit
TAGTATGGGGGTGGGGGAGGAGGGACCGGCGGCGCCCACGTGGCCTCCGCGG630.10720849499693691No Hit
TTCTTGGGGGAAAGTAGAAAACTCCTATGTGTTTATCACATTGCAGGGCTTT630.10720849499693691No Hit
TGCCATTGGGTGCATTTTCTTTAAATGTTTTGTTCACAACCTTGTCATTATT630.10720849499693691No Hit
CGCCGAGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA630.10720849499693691No Hit
CACTTTGGGGAAGTGAAAGCAAGTTTAAAAGCCCCTGAAGTTTGTTGTAGCT620.10550677285412838No Hit
AGTGAAGGGATCCTACAGGTGGCTGGAGCAGCTGCTTTGCAACCTGATCACC620.10550677285412838No Hit
GACCTGGGCATCCTCTGTTTATTATAATTTTAGCACCAACTTCACACCTAGC620.10550677285412838No Hit
GCTGCTGGGGGGCTGCGTCGGGCTGCAGGAGAAGATGGCGGTCTCCACAGGA620.10550677285412838No Hit
GTTCCTGGGGCGCAGGGATGGAGCCGGAAGAGGGGACGCCCTTGTGGCGGCT610.10380505071131986No Hit
TTCGAAGGGACGACATGTATAGGTTACCTAGATGTTATGAGAAATTTTAGTT610.10380505071131986No Hit
TAATTTGGGGTGAGGGAGGAGACAGGCCCAAGGGGGAGGCCCTCCGAGGGAG610.10380505071131986No Hit
CATTGTGGGAATGGCAAAGACCGCAATTATTTTTGTACCAACATAATACACC610.10380505071131986No Hit
AATCCTGGGGTTGTCAGGAATAGTCCGGGCTATTGGTCTGATTCAGATGGGC610.10380505071131986No Hit
ACATTAGGGAAGCACATATGGCTGGACAGCAAACATGGAGAGAATCATGAAA610.10380505071131986No Hit
TTTGCAGGGGAATTGTAGCTCCTATAGTCCCCACGGGTCATGGGAGGGACCT610.10380505071131986No Hit
GATGAGGGGTGTTTTAGCATTACTGTGTAAAGACTTGAAGGGACAGAAGTGA610.10380505071131986No Hit
AGCAGAGGGGCCAGAAGACAGTGGAACAATATCTAGAGTGTTAAGGGAAAAT610.10380505071131986No Hit
ACAGCTGGGCCCCCAAAAAAGGCTTGATGTTGTCACAGAAGATTTTATAAAT610.10380505071131986No Hit
TTTCGAGGGATGCGCGGCGCGGGGGCGATCCTGCGGCCGGCGGCGCGTGGTG610.10380505071131986No Hit
TAATCTGGGAGATCGAGGTGTTGGTGGACGCCGACCCTGTTGTGGACAGCTC600.10210332856851133No Hit
GATAATGGGTAAGCCCTTAAAATTAGAGTCATGTGCCAAGCTACATGACTCT600.10210332856851133No Hit
ATATTTGGGGACCTTGAGGCTGATGAGGGGACCCCTGCCTGTTTCCCCCATA600.10210332856851133No Hit
ATCGCGGGCATCCCTCTCAGGGAGGCCAGCCTGAACTGGGCCTGGTTTACAG600.10210332856851133No Hit
GCCAAGGGGCCTGTGTGCAGACTCCACAGAGGGGGAAGAGCCAAGCTCTTTT600.10210332856851133No Hit
ACCATTGGGAGGAGGCGTAGGAGCGGCCCTGCGGGCCCTTTCACGGCAGCTG600.10210332856851133No Hit
TAAGACGGAGAAAGTGAACTTGGCATTTTAGATCTAATTCAAGTATTTGTGG600.10210332856851133No Hit
TGCTTGGGGAGAAACAAAATTTTGGAAGCTGAAAAGCAGGTAGATGAGTGGT600.10210332856851133No Hit
GGCTCCGGGCAGCTTATTATTCTCAATTGCTAAATACGATTACTCTGCTTTT600.10210332856851133No Hit
GAGTTTGGGATGTACTAAGCAAAGAAGCCTTGAAAAGATTTGTTGATGCATT590.1004016064257028No Hit
AGCCACCAGAGCCCATGTGCGCACTAGCTTTTATATTTCAAGCTGTGTTTTA590.1004016064257028No Hit
CTCACCGGGGGATGGAGCCAACCTGGGTATTCACAACAGGCCTGACTTGATA590.1004016064257028No Hit
TTGAAGGGGCTAGACACTGTCCCAGGGAAAGTAATAGAAGGTGGAAGAAATC590.1004016064257028No Hit
TGCCGAGGGATAACAGCCACATGTGATGTAAGTCATATCACTCTGGCAAAAA590.1004016064257028No Hit
GTAGTTGGGCAAACACATTATTTTGAATACCTTTTTTTCAACTTCATACATT590.1004016064257028No Hit
ATCATAGGGAAGGACTCTCACCCGAGAGCTGGCTTCTGCCACTTGCCCTGCC590.1004016064257028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)