FastQCFastQC Report
Sat 31 Dec 2022
SRR5844027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58731
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC2150.3660758372920604No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT1710.291157991520662RNA PCR Primer, Index 17 (95% over 22bp)
TAAAAGCGGGAGCACGGTTCCTGAGACATAGTAGCCACTCAATAAATGTTAG970.16515979635967376No Hit
TAGGCAGGGGGAATACAACGAAAAAGACAATTTTTAAATAGTTAAAAGTGTT930.15834908310772847No Hit
CTATATGGGTGAGGGGTCTTTAGTAATGCATCTGGGTAGCATCTCAACTGCT910.1549437264817558No Hit
CCGAACGGGAGTCTGGGACGCGCCGCCGCCATGATCATCCCTGTACGCTGCT860.14643033491682417No Hit
CAGTTCGGGTCTTGCCTTTGGGGAGCTCCCAAGGGAAAAAATAATATAATGA820.13961962166487885No Hit
GCAACTGGGCTTCAAAGGATACCATCACATTTTATAATTCATAGATCTGATA820.13961962166487885No Hit
TGCGCAGGGGGACAAGTTCACTGAGAGCGTCTACGTCCTGGCCAACGAGCCA810.13791694335189253No Hit
GTACGTGGGGTTTATTGTTTAATTTATGCCCAAGTGAAGTTGTAAACTTATG800.1362142650389062No Hit
CCGCGTGGGATGATTATTTTTATAATCAGAAAAGTTATAAACAGTCCCACTG780.13280890841293355No Hit
TTTTGAGGGCATTTCCAAGGTGCAAGTGTTTATTTTTCAAATCTATAAGTAC780.13280890841293355No Hit
TCCTTAGGGGTACCTAGATGACAATGTTTCCATTCTGAAAAATAGAAACATA780.13280890841293355No Hit
ATCCAGGGGATCCATTCTCTGGTACAGTCAGACCAAGAAGCTCAGGCCGTGG750.12770087347397457No Hit
TCAGCCAGCAGCCCCAAGCCCGACACCAAGGTGCCCCAGGTCACCACCGAGG740.12599819516098823No Hit
ATCAGAGGGAACTTTGGGAATGAATAAAGTGGAATGGTAACTTTCCAGTGGT740.12599819516098823No Hit
TGACAAGGGAAGCGGAAAATAATGATCAAGCGGCACGAGGTGGAGCAGCAGA740.12599819516098823No Hit
GTTCTCGGGACCACGCCGAGCTCACCGTGCCCGTCCTGCTTGTCCACGGCAT720.12259283853501557No Hit
CTTGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA710.12089016022202925No Hit
ACAATAGGGAAGTTCAATGTATGGGACACAGCCGGCCAGGAGAAATTCGGTG710.12089016022202925No Hit
GTTTAAGGGATACTTCGGCAAACTTATTGGGTCTTCATTTTCTGACAGACAG700.11918748190904292No Hit
TCTACTGGGCAAACAGAGATTAAATTTGTGTATGCACAGACCTCTTTTGTTT700.11918748190904292No Hit
CCGACTGGGTATCTGAACTCATCACAATTTCACCCTGAAATAATGTGAGAAC700.11918748190904292No Hit
AACCACGGGGCTGTTTTTGATGGACATGGAGGAATTCGAGCCTCAAAATTTG700.11918748190904292No Hit
AATCCTGGGGTTGTCAGGAATAGTCCGGGCTATTGGTCTGATTCAGATGGGC700.11918748190904292No Hit
GAATTGGGGATGATTGAATATTCTTCTGCAAATTTCTTCTGTTAAAATTTTG700.11918748190904292No Hit
GCCAAGGGGCCTGTGTGCAGACTCCACAGAGGGGGAAGAGCCAAGCTCTTTT700.11918748190904292No Hit
ACAATAGGGGTCAGAAGAGTGCTCGGTTAGCCCACGGTGCCAAAACTCCAAA700.11918748190904292No Hit
TAAGACGGAGAAAGTGAACTTGGCATTTTAGATCTAATTCAAGTATTTGTGG700.11918748190904292No Hit
AGCCACCAGAGCCCATGTGCGCACTAGCTTTTATATTTCAAGCTGTGTTTTA690.11748480359605659No Hit
TTAAATGGGATTCTTCCCGGGGAGACCGCAGCCCATCGGCATGGCTCAAGAG690.11748480359605659No Hit
TCTACAGGGACCACCGTATGGACAGCCACAGCCTGGCTTTGGGTACAGCATG680.11578212528307026No Hit
TAAATGGGTTTGGGATTACATAAATGACAATTACAGAAAGATTTTAACCATT680.11578212528307026No Hit
AGTGCGGGATTCTCTAGGAACTCACTTTTGAGAGTATTTTTCTCTAACCCAT680.11578212528307026No Hit
AAAATAGGGGTTTAATTTGGGTTGTGGTTATATTGTTTTTTCACATCACGTG680.11578212528307026No Hit
GGCAATGGGAGCTGGTGCAGTGGTTCTGGCACACGCTGGAAGAGTTCTCCAA680.11578212528307026No Hit
CATGGTGGGGCTACTCTTTTGGCTAAGACATCTTTGGTTTCTGCCATTTGCC680.11578212528307026No Hit
TTGTTCAGGGGCCTTTTCAAAGTCAAAAGAAAGATTTTGGAAAGCATTGCAT680.11578212528307026No Hit
TTCCTAGGGGGCTTCCTTCTGCAACAGTCGTGGGTCACGCTCTCGCTCGGTC670.11407944697008396No Hit
CCGTTTGGGGAATAATTAAAATAAATTTAAATTCAAATATTAACATTTCAAT670.11407944697008396No Hit
ATAATAGGGCATCACTGGTCATTAGAGAAATGCAAATCAAAACCACAATGAG670.11407944697008396No Hit
TACCACAGGGTAACTGTCATCTTGTGAGAGGCAACCTCAAACTCTAAGCAGA670.11407944697008396No Hit
GGCTCCGGGCAGCTTATTATTCTCAATTGCTAAATACGATTACTCTGCTTTT670.11407944697008396No Hit
GCAATTGGGCGATCTCTGAACGGGAGCCTGGCTTTGGGTACAGCATGTGAGA670.11407944697008396No Hit
TGAATAGGGGTTAGCATCAGTGACCTCCTGGTACCAAAAGACTTGGGAACAG660.11237676865709761No Hit
GGGAGGAAGTTCAAGGAAGGCACCACACCAGAAATGGGACCTAAATTAAGGC660.11237676865709761No Hit
CTACCCGGGTAAAATACAGCCCGAACTGCAAGTTGCTTATTGTTTCAAATCC660.11237676865709761No Hit
GTCATGGGGAATCAAGAGTTAACAAACCTAACGTAACCTGAGGTTTAATATT660.11237676865709761No Hit
ATATTTGGGGACCTTGAGGCTGATGAGGGGACCCCTGCCTGTTTCCCCCATA660.11237676865709761No Hit
GCTTGGGTATACTTGGACCCCATGGCTGTGGTGGCAGAAGGAGTGCCCTGGT660.11237676865709761No Hit
GATGAGGGGTGTTTTAGCATTACTGTGTAAAGACTTGAAGGGACAGAAGTGA660.11237676865709761No Hit
GACCTGGGCATCCTCTGTTTATTATAATTTTAGCACCAACTTCACACCTAGC660.11237676865709761No Hit
GATGCTGGGCTTTGAAAGCAATTAGGTTATTCACCTGGTATTAAAACTATTT660.11237676865709761No Hit
ACACATGGGCAGCCTGGGCATACTTTCCCATCAGAGTCAGCCTCTATCACAA660.11237676865709761No Hit
ATTATGGGCGTAGCGGGGTTGCAGGAAGAAAATCGACTGTTAAAACAGAAGG650.11067409034411128No Hit
GTCGCAGGGACTTTGGGAAGTTATTTCTTTATTTTAATGATGTATCATTGTT650.11067409034411128No Hit
ACAGCTGGGCCCCCAAAAAAGGCTTGATGTTGTCACAGAAGATTTTATAAAT650.11067409034411128No Hit
GCTGTTGGGTGTGCTGAATACATCAATGAGGCGGCTGCGATGAACTGGAGAT650.11067409034411128No Hit
ATAAGCGGGATTAAAAAAACAGGATGTAATGTCCTTCTCATACAGAAATCTA650.11067409034411128No Hit
GATTCGGGGGCAGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGCTGGCCGT640.10897141203112495No Hit
GGTGATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG640.10897141203112495No Hit
CGTTCAGGGATACTTGAAGGCCCAGAACACTCATGGCACATTTGTTTAATAT640.10897141203112495No Hit
CTACCGGGGAATCGCCGAGGGCACGTGCATGCCCCCTGGTTAAGAGTTGCAG640.10897141203112495No Hit
GCTGCTGGGGGGCTGCGTCGGGCTGCAGGAGAAGATGGCGGTCTCCACAGGA640.10897141203112495No Hit
TGCAGCGGGGTGCTGTAAGTGCCATTTCTCAGTATTTTCAAGGCTCTAACCC640.10897141203112495No Hit
TAATCTGGGAGATCGAGGTGTTGGTGGACGCCGACCCTGTTGTGGACAGCTC630.10726873371813862No Hit
TTCCTTGGGGAAAAAACAGCAGATGGCTCGAGAATACAGAGAGAAAATTGAG630.10726873371813862No Hit
TAATTTGGGGTGAGGGAGGAGACAGGCCCAAGGGGGAGGCCCTCCGAGGGAG630.10726873371813862No Hit
GCGTACGGGATGCCCAGCCAGCCACCCCTGCCACTCACCCCTCCTGGCCCAG630.10726873371813862No Hit
ACTGGTGGGATTTCAGTTAAAAGGCAGAGATTATCAGATTGGCTAGAAAAGA630.10726873371813862No Hit
TCGGATGGGGATAGCTGTGCAGCAGGCCTCGAAAGCAATCGGTCCAAATTAG630.10726873371813862No Hit
TGCGGCGGGAGCATTTTTAATTTTCACAAAATGTCTCAGTTTTCAGCTATAC630.10726873371813862No Hit
CAGTATGGGAGTGAGACTGACTGCGTCGGGGTTGAGACTGGGTGGATGAGGC630.10726873371813862No Hit
GCACATGGGAAATATGAATGTAGGGAAGCTGTAAGCCAGCCCATATACAGAA620.1055660554051523No Hit
GTTCCTGGGGCGCAGGGATGGAGCCGGAAGAGGGGACGCCCTTGTGGCGGCT620.1055660554051523No Hit
TGACCAGGGAGGAAGCTGGAGAAACAGGAGAAGAAACGCTTAAAGAAGGAAA620.1055660554051523No Hit
TTAATAGGGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATG620.1055660554051523No Hit
AGCTTTGGGCCAGTCCAAGAACCTGCCTAAAGCCGACAGACAGGCCCTGAAT620.1055660554051523No Hit
AAACCAGGATTTCGGGTCTGCTAGGAGTCCCAGAGGGTTTTCTCTTTTTGAT620.1055660554051523No Hit
TCAGCGGGGAGACATCTCACTACTACTACTGACTATCACACACTGGTTGCCA620.1055660554051523No Hit
CAAACTGGGAGGGTTAAAAATGCTGAGTGAATACTGCAAATATACCTTAGCA620.1055660554051523No Hit
GAAGCTGGGGTTAAGATAATTATATTGCCACTAATAATCAAGATGCTAAATG620.1055660554051523No Hit
ATCGCTGGGGTTGCAAATTTGTTAAAATGCCAAAAAGGACAGTTTAATTTTG620.1055660554051523No Hit
ACTGCAGGGCAGTGGAATGTGTAAGCTAGCTAATTCTTGTTTTCTGATTTAA620.1055660554051523No Hit
CAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG610.10386337709216598No Hit
GGGCGGGGGAGGTAATATTTTTGTATCACAATGCATTATTTTTTTCCTCCTT610.10386337709216598No Hit
CAAAACGGGTACCAGTCTTATTCAGAAAGTGGATCCCTGGCATTTCTCTTTC610.10386337709216598No Hit
GTTATAGGGAGATCCAACTTTCAGTGGGCTTGGTTGGGTTATTTTGTTGCCA610.10386337709216598No Hit
GCACTGGGGTTGAATATGATTTGGTCAGTTGCTCGTTGTAACTTGGAGAAAT610.10386337709216598No Hit
AGCAAGGGATTCAGAACCTGTTAGATTAAAGCTGCACCCTGTGATTATTTGA610.10386337709216598No Hit
GTTGGTGGGCTTTGAACTAGACAGTTAAACTTCTCATATTTTAAATTTTTTT610.10386337709216598No Hit
CGCAGTGGGGCAGAAGATTTGTTGCCGTCATGTCGGCTGCGATTGCAGCTCT600.10216069877917965No Hit
GACCGGGACATGTTTTTATAATAGGGATGCAGGCATTCCAAAATAAATGAAT600.10216069877917965No Hit
ATTATCGGGACATGACCAATCTAAGTTTATTTCTTACTCAATATAATAACCA600.10216069877917965No Hit
TGTACGAGGGAGGGAATTGGGCTGGAAGAATGGTGGAATGGAATAGCAAAGA600.10216069877917965No Hit
TTTTGCGGGAATGCATTTGTCTTGAATGCTTAATATATTTAATGGGAAATGT600.10216069877917965No Hit
AGTTTGGGGGGAAAATATCACAGTCCTAAATATATATGCACCTAATACTGGA600.10216069877917965No Hit
ATAGTTGGGAGTGACTCATTATGGAGGAATTAGAAGAAACATTTTGGTATAT600.10216069877917965No Hit
AAGTCAGGGTGCAAGGAAGAGGCTTAAGTATGTTCATTTTTATCAGAATGCA600.10216069877917965No Hit
GAACCGGGACATGGCACTGAGTGTACTCTCTGCCAGTGCAAGAATGGCCACA600.10216069877917965No Hit
CTCATCAGGGGAAACTGGAATTCCTGTCGTTACTGTTTCCTTATCAAAGAAG600.10216069877917965No Hit
CATCAGGGGCCGTTGAAAGGCGCTTCACAGTGCTTCAACCTGTAGCTTCAGC600.10216069877917965No Hit
TGCCATTGGGTGCATTTTCTTTAAATGTTTTGTTCACAACCTTGTCATTATT600.10216069877917965No Hit
TATCTTGGGATCTCTCAACTCTCAGCACAGTGCTACTGCGAAACTGATGGGA600.10216069877917965No Hit
CATGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA590.10045802046619332No Hit
ACCGTGGGCCACGGAGTCCCTCCCAGGACACATGGGGATTACGGGAACTACA590.10045802046619332No Hit
CAAATGGGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGT590.10045802046619332No Hit
ATCACTGGGCTTGTGGGTTCTTTAGGGTTTGAGTTTCTTCTTCCCCTTGAGC590.10045802046619332No Hit
TTCGCGGGGGGGCCCTTATTTGCTGTTCTAAGAGTTGTCTGTAGGGGTTTTT590.10045802046619332No Hit
GCATATGGGCCAAGCCGCGCTACATGCAGTGGCGGGAAGGCATCAGCTCCAG590.10045802046619332No Hit
AAGTTAGGGAGAATGAGGAATGAGGGAAGAAGAGAAATTAAGGGTGTCTCCT590.10045802046619332No Hit
ACCATTGGGAGGAGGCGTAGGAGCGGCCCTGCGGGCCCTTTCACGGCAGCTG590.10045802046619332No Hit
TTTAGCGGGCTGCTGGGTATGTCCTAGTTCCAGACTCACTGGTTATACGGTA590.10045802046619332No Hit
TACTTGGGGGGGCATTGTTTGGGGTGAGAAAAAGTTGATAAAACCTAAGGGA590.10045802046619332No Hit
ACCTAGGGAAAATGCTTTGCTGGAGCTAAGTAAGAAGCGAGAATCTGTTCCT590.10045802046619332No Hit
GCACTGGGGTCTCAAGAAGGGGGAGGCTAAAAACCTGAAGAAGATGGACGTA590.10045802046619332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)