FastQCFastQC Report
Sat 31 Dec 2022
SRR5844026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58252
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT1910.3278857378287441RNA PCR Primer, Index 17 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC1860.31930234155050474No Hit
AGTGCGGGATTCTCTAGGAACTCACTTTTGAGAGTATTTTTCTCTAACCCAT850.14591773673006936No Hit
AGAGTGGGGCAATGAGACAGCTAAAAGTTAGTATGTGGGGGGATGGAGTTAA830.1424843782187736No Hit
ACAGCTGGGCCCCCAAAAAAGGCTTGATGTTGTCACAGAAGATTTTATAAAT820.14076769896312571No Hit
CCGCGTGGGATGATTATTTTTATAATCAGAAAAGTTATAAACAGTCCCACTG810.13905101970747785No Hit
AGCAAGGGATTCAGAACCTGTTAGATTAAAGCTGCACCCTGTGATTATTTGA800.13733434045183No Hit
GTACGTGGGGTTTATTGTTTAATTTATGCCCAAGTGAAGTTGTAAACTTATG790.1356176611961821No Hit
TGACAAGGGAAGCGGAAAATAATGATCAAGCGGCACGAGGTGGAGCAGCAGA790.1356176611961821No Hit
GCACTGGGGTTGAATATGATTTGGTCAGTTGCTCGTTGTAACTTGGAGAAAT780.13390098194053424No Hit
TCTACAGGGACCACCGTATGGACAGCCACAGCCTGGCTTTGGGTACAGCATG770.13218430268488635No Hit
ACTACTGGGTGTGCAGTATTTGTTTTTGTTGTCTTGTTGAGAAATATCCCCT760.13046762342923848No Hit
GAAGCTGGGGTTAAGATAATTATATTGCCACTAATAATCAAGATGCTAAATG760.13046762342923848No Hit
CCGTTTGGGGAATAATTAAAATAAATTTAAATTCAAATATTAACATTTCAAT740.12703426491794273No Hit
CTATATGGGTGAGGGGTCTTTAGTAATGCATCTGGGTAGCATCTCAACTGCT740.12703426491794273No Hit
TTTTGAGGGCATTTCCAAGGTGCAAGTGTTTATTTTTCAAATCTATAAGTAC740.12703426491794273No Hit
GATGAGGGGTGTTTTAGCATTACTGTGTAAAGACTTGAAGGGACAGAAGTGA730.12531758566229487No Hit
TCAGCGGGGAGACATCTCACTACTACTACTGACTATCACACACTGGTTGCCA720.12360090640664698No Hit
TAAGACGGAGAAAGTGAACTTGGCATTTTAGATCTAATTCAAGTATTTGTGG720.12360090640664698No Hit
ATTCTAGGGCAAGTAATCCTAGACCCCAATATATAAAAGAAACCAACTCATC720.12360090640664698No Hit
TAGTATGGGGGTGGGGGAGGAGGGACCGGCGGCGCCCACGTGGCCTCCGCGG710.12188422715099911No Hit
CCGAACGGGAGTCTGGGACGCGCCGCCGCCATGATCATCCCTGTACGCTGCT710.12188422715099911No Hit
ATCACTGGGCTTGTGGGTTCTTTAGGGTTTGAGTTTCTTCTTCCCCTTGAGC710.12188422715099911No Hit
ATGCCTGGGATAACCATTGAGGAGAGTCTTAGAAGTTGTATTTTAAGGAATG710.12188422715099911No Hit
AGTTTGGGGGGAAAATATCACAGTCCTAAATATATATGCACCTAATACTGGA700.12016754789535124No Hit
TCCTTAGGGGTACCTAGATGACAATGTTTCCATTCTGAAAAATAGAAACATA700.12016754789535124No Hit
CTTGCGGGGAAGCAGCTTTAAAAAACTGCTGGCAGAACTAAGAAGTCAGACT690.11845086863970335No Hit
CAAACTGGGAGGGTTAAAAATGCTGAGTGAATACTGCAAATATACCTTAGCA690.11845086863970335No Hit
GCTGCTGGGGGGCTGCGTCGGGCTGCAGGAGAAGATGGCGGTCTCCACAGGA690.11845086863970335No Hit
TCGCTGGGGAGTTGCTGGTGGGCAGCCCTGCTTCCTGCATGGAACTGGAGCT680.11673418938405548No Hit
AAACATGGGATGAGAGGAAGTATTTGAAAGGTATATATCTGATAGGGGATTA680.11673418938405548No Hit
GGCAGTGGGAGGAGTTCATAAGTGTATTTAATGAAATTGGTGGTTTTAGGAA670.1150175101284076No Hit
TGACCAGGGAGGAAGCTGGAGAAACAGGAGAAGAAACGCTTAAAGAAGGAAA670.1150175101284076No Hit
GTCATGGGGAATCAAGAGTTAACAAACCTAACGTAACCTGAGGTTTAATATT670.1150175101284076No Hit
CCGACTGGGTATCTGAACTCATCACAATTTCACCCTGAAATAATGTGAGAAC670.1150175101284076No Hit
GCCAGTGGGAACATATTTGTCTTTATGGTTGTGTTTATTACATGTGCAGTGA670.1150175101284076No Hit
CAAAACGGGTACCAGTCTTATTCAGAAAGTGGATCCCTGGCATTTCTCTTTC660.11330083087275973No Hit
CTACGAGGGATTTGTTCAGACACTGAATCTTAATTTTACCATTTCCCTTTTT660.11330083087275973No Hit
GTTTGCGGGCTCTGCGTCAGGGAGCACTTTTTTGTTTGCCTCGAGACTACTC660.11330083087275973No Hit
GACCTGGGCATCCTCTGTTTATTATAATTTTAGCACCAACTTCACACCTAGC660.11330083087275973No Hit
GTTTAAGGGATACTTCGGCAAACTTATTGGGTCTTCATTTTCTGACAGACAG650.11158415161711187No Hit
TAATTTGGGGTGAGGGAGGAGACAGGCCCAAGGGGGAGGCCCTCCGAGGGAG650.11158415161711187No Hit
CTTGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA650.11158415161711187No Hit
TGACGGGGGGCTACAACCCTGGGCAAGTTACTTCACCTCTGAGCCTCCGATG650.11158415161711187No Hit
TTCGCGGGGGGGCCCTTATTTGCTGTTCTAAGAGTTGTCTGTAGGGGTTTTT650.11158415161711187No Hit
ATGCCAGGGGGAGCGCCGGGGAAGCGAAGTAGGCAGGGGCGAGGCGGCTGGG650.11158415161711187No Hit
TAAAAGCGGGAGCACGGTTCCTGAGACATAGTAGCCACTCAATAAATGTTAG650.11158415161711187No Hit
TGCCATTGGGTGCATTTTCTTTAAATGTTTTGTTCACAACCTTGTCATTATT650.11158415161711187No Hit
GCAATTGGGCGATCTCTGAACGGGAGCCTGGCTTTGGGTACAGCATGTGAGA650.11158415161711187No Hit
TGAATAGGGGTTAGCATCAGTGACCTCCTGGTACCAAAAGACTTGGGAACAG640.10986747236146398No Hit
GAATTGGGGATGATTGAATATTCTTCTGCAAATTTCTTCTGTTAAAATTTTG640.10986747236146398No Hit
TTTGCAGGGGAATTGTAGCTCCTATAGTCCCCACGGGTCATGGGAGGGACCT640.10986747236146398No Hit
AGGACTGGGCTCTCTAAATCAGGTAGCACGAGTCTTCCAACTTTGCTTTTTC640.10986747236146398No Hit
GGCAATGGGAGCTGGTGCAGTGGTTCTGGCACACGCTGGAAGAGTTCTCCAA640.10986747236146398No Hit
TAATCTGGGAGATCGAGGTGTTGGTGGACGCCGACCCTGTTGTGGACAGCTC630.10815079310581611No Hit
TGGCCGGGGACTTGGTCAAAATTAAGCTATATATTGATGATATGTGTACTTT630.10815079310581611No Hit
CTTTTGAGGGAAGGAGGGCTACTATGAGTTTGAGTCTCCATTCTGGGATGAC630.10815079310581611No Hit
TGCGCAGGGGGACAAGTTCACTGAGAGCGTCTACGTCCTGGCCAACGAGCCA630.10815079310581611No Hit
TAATACGGGACATTTCAATCATGAAGGAAAAATAGAGACCTTCCCAGAAAAA630.10815079310581611No Hit
TGCAGCGGGGTGCTGTAAGTGCCATTTCTCAGTATTTTCAAGGCTCTAACCC630.10815079310581611No Hit
ACACATGGGCAGCCTGGGCATACTTTCCCATCAGAGTCAGCCTCTATCACAA630.10815079310581611No Hit
CTAATCGTGGGATCCATATCCTTAGAAGACAATAAAAAAGAGGATTCTAAAT620.10643411385016822No Hit
ACCATTGGGAGGAGGCGTAGGAGCGGCCCTGCGGGCCCTTTCACGGCAGCTG620.10643411385016822No Hit
GGCATAGGGATAATGAATAAATGTTCTTGCTTTGTATGGAAATACAATTCTT610.10471743459452036No Hit
TGTCCGGGGTATTCATTCAGAATGCTTTAAGCTTAGTCATAAACTACATTTG610.10471743459452036No Hit
GCGTACGGGATGCCCAGCCAGCCACCCCTGCCACTCACCCCTCCTGGCCCAG610.10471743459452036No Hit
TAAGTTGGGGGAGGAAGAGGGCGGCGACGGTGGTGGTGACTGAGCGGAGCCC610.10471743459452036No Hit
TAGCTAGGGAATGCTCAGGGAGAGTGATTTGAGTAATAATGAAACTGTTGTC610.10471743459452036No Hit
CTAGCTGGGCATATATATATATTCCTAGACATCTAGTGTTTGCTGTCATTAG610.10471743459452036No Hit
GCTTTAGGGGACTAGCCTTTTAAAAAAAAGAAAGAAAATAGAGAAAATCTTA610.10471743459452036No Hit
CAAGCCGGGGAGCCTTGATTACCATAGGACAGGCAGAAAGGCGATAATTTGT610.10471743459452036No Hit
GATGCTGGGCTTTGAAAGCAATTAGGTTATTCACCTGGTATTAAAACTATTT610.10471743459452036No Hit
TTCCTTGGGGAAAAAACAGCAGATGGCTCGAGAATACAGAGAGAAAATTGAG600.10300075533887249No Hit
AACGGGGCCGTGAAAGTTGGAAGTGTTGATTGCCTTCAGGAAGAGATTGATG600.10300075533887249No Hit
CTACCCGGGTAAAATACAGCCCGAACTGCAAGTTGCTTATTGTTTCAAATCC600.10300075533887249No Hit
GTCGCAGGGACTTTGGGAAGTTATTTCTTTATTTTAATGATGTATCATTGTT600.10300075533887249No Hit
GTCACAGGGATCCCCATGAGCCCCTCCTCCATCCAGCGACAAGCCATGGGCA600.10300075533887249No Hit
TACCACAGGGTAACTGTCATCTTGTGAGAGGCAACCTCAAACTCTAAGCAGA600.10300075533887249No Hit
GGCTCCGGGCAGCTTATTATTCTCAATTGCTAAATACGATTACTCTGCTTTT600.10300075533887249No Hit
TTACAAGGGATATGAGAAAGTAGAGAGGGAAATGGGGAATGATTGGTACAAA590.10128407608322461No Hit
CAAATGGGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGT590.10128407608322461No Hit
TCAAAGGGGATATAAAATAGTATCTTTATACCACAAACATCCTCTCCCTTTT590.10128407608322461No Hit
CACTAAGGGAGTCAAGGAATTTGCCAAGTATGATGTGGATCCCCAGAAGTGG590.10128407608322461No Hit
ATAACGGGTGGCAGGATACTGCAAAGAACATGGTTTATTTTAAAATTTATAA590.10128407608322461No Hit
TATCTTGGGATCTCTCAACTCTCAGCACAGTGCTACTGCGAAACTGATGGGA590.10128407608322461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)