FastQCFastQC Report
Sat 31 Dec 2022
SRR5844025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128172
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC4350.33938769778110667No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT3640.2839933838903973RNA PCR Primer, Index 17 (95% over 22bp)
CCAGATGGAGCACCTCTGCCCGGCCCATCATCTGGGAGGTGGGGAGCACCTC1940.15135911119433262No Hit
CGTGGCGGGCGATCTGTCGGTGGGGATGCAGAGCGGAGGCTTCGCGCAGCAG1930.15057890959023812No Hit
AAACATGGGACCTTTCTTTCTTCTTGTGAGGACATCTTGAAAAGACTATCAT1870.14589769996567112No Hit
GTTTTGGGGATGTATGACCTGGATGGTGATGGCAAGATCACCCGAGTGGAGA1850.14433729675748214No Hit
TCCTCAGGGGATCTGTAAGCGGGACCCCAAAACTTCAATCTGAAATGTCTTA1840.14355709515338763No Hit
CGGCGCGGGCGATCTAGGATGGGGAACGTCACTACATGTGCTTATGCAAAAA1770.13809568392472615No Hit
AAACTAGGGGGTTCCTGGCTAGGGAGGCGGCCTTGGCTGGCTTGACCGTCAT1760.13731548232063165No Hit
TGAGATGGGAGCTGTTTTTGGTACCATTTTAATTTTTGCAACTTACTTGTAA1750.13653528071653714No Hit
TTCACTGGGAGTGGCATTACACCAAAATTTCTTAGTAGGACTCCTGCATAGA1710.13341447430015918No Hit
GCTACTGGGAGTCCTCAAAATATTTTGCACGTCAGTGTCATAAATTCCTATA1680.13107386948787567No Hit
TTAGCTGGGGTAACTGCTAATCTACATACTCTCAAGTCACTAACCTTCCTCT1670.13029366788378116No Hit
AGATGGGGGGCCGCGCCGGCTCTGGGCACTCAGCATCGTTTCCTTTTCCTCC1640.12795306307149767No Hit
ACTCGCGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1620.12639265986330867No Hit
TACGTTGGGGAGAGGGTTGCGGGCTAATCCAAAAGAATTGGTTTATAATTAT1600.12483225665511968No Hit
AGTAGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1580.1232718534469307No Hit
CGGTCTCCTAAAAGAAAATTGTCCCGCTCACCATCCCCTAGGAGACATAAAA1570.12249165184283618No Hit
TAAGCTGGGCAGAGGACTCATGGTGATACAGCTGATGTAGCAAATGGATTTG1560.12171145023874169No Hit
AGCATAGGGAACTCCAGCACCATCTGCTCCGTTTCTTGAAGTTTGCTGAACG1550.12093124863464719No Hit
ACAACCGGGGATACCAGTGCTGCTGTAACTGAAGAAATGCCACCCCTTGAAG1520.11859064382236371No Hit
AAGTACGGGATTGAACACTTACTGGAGCAAATACTTAAACTACTAGCTTGCT1520.11859064382236371No Hit
GTGGCTGGGCAAGCTGGTAAGGTGCTGTTCACTTGAACAGGTGCTGTTGCGC1510.1178104422182692No Hit
CGACAGGGGAGCCAAAGAGGTCGAAGGGGTTCTGGAAACTTTGGTGGTGGTC1500.1170302406141747No Hit
TTTACAGGGAGACTTATGAGAAATCCAAGATTTCCAATGCAAATTTAATTCA1490.1162500390100802No Hit
ACCAAGGGGAACAAGCACCTGGAAGTGCGGCACCAGCGGAGCGGGCGTGGGG1490.1162500390100802No Hit
GTAATGGGGTCAGCTTGGGAGGAGGAGCTGATGCTGACGATCTCCCCAACAT1480.11546983740598571No Hit
GCATTTGGGGGGGGTTTCAGTGGCTTCTGGTGCTCTAGGGTGAGCTCTGCCC1480.11546983740598571No Hit
TGAAATGGGAGAGGTTATGTCATTTTATAAAGACTTCATTGATAAGATGGCT1470.11468963580189122No Hit
CTCGCTGGGGTGCCACCGCAGGCAGCGCTCCAGTCGGGAATGGCCAGGATGG1450.11312923259370221No Hit
AGCGCGGGGCCGATCTATAGCAGGGGAACTTGGCTCAGCTGCCGGGCTGCTC1440.11234903098960772No Hit
GGATAGGGGCCATTTTAATTAAACAATGCTTCAAGGCTGCATATTAAATAGA1440.11234903098960772No Hit
AATTTGGGGAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCT1440.11234903098960772No Hit
CTATTTGTGGGATTTATTTTTGGAATTCAAGGATGATTCAACATATGAAAAT1430.11156882938551321No Hit
ATAGCTGGGGCCTGCAGGCACTATAATCCACCTAGACAATCAAGTCTTCATA1430.11156882938551321No Hit
GTTCAAGAGGGTAATGAAGAATCTTAGGCGGGTGCACCCAGTTTCCACCATG1430.11156882938551321No Hit
AAGGTCGGGCAGCCCCACCCAGGGTCTCCTGGGAAGACCAGCCCTTCCAACT1420.11078862778141872No Hit
GCGGCCGGGGGGAACCAGAACATATAAAAAAATGTAAATTCCAGAGTTGAAA1420.11078862778141872No Hit
TTATGCGGGGGGGGACCAAGGAGAAACTTTTACCCAAAGCCCTATTACCTCC1410.11000842617732423No Hit
GAGTGCGGGGTATTTAAAGGCAAGATTTTGATCTTCAAAAATTTTTTTCTCC1410.11000842617732423No Hit
ACATCAGGGAGTCATCCCTTGACTTTTCAAATGACAGAATTTTTTCCAATTG1410.11000842617732423No Hit
CCTAGGGGGAGAGCTCTGATCCTAACCACCACCACCTCTCCCTTCAGCAAAA1400.10922822457322973No Hit
TGCATCGGGGAGTCTCTCCGCAGCCGCGCGGCCACTGCGAGCCCAGCCGCAG1390.10844802296913522No Hit
ATGAACGGGCTTGGTCCTTGGAAGACCTATATTCTCTGTAGTTACTGAAAAC1390.10844802296913522No Hit
TCATCTGGTCTATGCAGGGGCTTCCGATCTTGGTGGGGAGTGGAAATGTTTG1390.10844802296913522No Hit
TGCATGGGGAGGTGTTGGCATTCTTCGCTGATTTGGCTGTTCCCAATGTTTA1380.10766782136504072No Hit
AGCATGGGATACTGTCGATATCATCCTTCCAAAGGGTATTGGTGGCACTTCA1380.10766782136504072No Hit
CGCTTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1380.10766782136504072No Hit
GTACAGGGGGCTGATCTTCAGTGAGGTCTGCATACCTGCCATCCTTACTGAT1370.10688761976094623No Hit
CTGTAGGGGGTGCCCACCCCTCAAGTGTAAGGAACTGCGTTCCGCCCCTCAG1360.10610741815685173No Hit
CGGTTTGGGAAACATGGTGAAGGTGATAGACCATGGATGCTTAGTCCCTTTT1360.10610741815685173No Hit
TTAACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1360.10610741815685173No Hit
TTCGCGGGGCGCTATCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTC1360.10610741815685173No Hit
TGATATGGGGACCCAGGGAGATTTAATCTACGTGTCTTGTAGCCTGTAATTT1350.10532721655275724No Hit
ACTAGGGGGTGGACAACGGTGCTTCCCCAACAGCTGATACCGCCGTCCTGGG1350.10532721655275724No Hit
ACAAATGGGATTCCACTTACAGTGCTGTCGGCGAGTATTAGAAAATCGATGG1350.10532721655275724No Hit
CGAGAGGGGGAAAAAAGTGGAGAAGTCTGTTAATGAAGTGATGGAATGGATG1350.10532721655275724No Hit
CGGACTGGGACTTTTTAACCTGAGAAAAAAACTTGGTTCTGCTTTATATAAA1340.10454701494866273No Hit
GATACGGGTGTCTGAAGTTACTTGAAGGCATCACTTTTAAGAAAGCTTACAG1340.10454701494866273No Hit
ATCCATGGGCACTATGTTGCACAGTAGAATAGAAATATGAATTCAGGTACTT1340.10454701494866273No Hit
ACAACTGGGATTTCCTGTGCTCACCAGAGGCAAAATGTACCAATATCCTGAC1330.10376681334456823No Hit
TACGCCGGGAGACGACATACATAAACTAAGAAACAAAAATATTGCTAGAGAT1330.10376681334456823No Hit
AACCTGGGGATGAAATGGAAGAAAAGGATATACTAGCTGTAGAAGATATGAG1320.10298661174047373No Hit
GGCAAGGGAGGTGAAAGAATAGAGTTTTATATCCAATTGAAGTTAAAGTTGT1310.10220641013637924No Hit
ATACATGGGTGGCAGCCTGAATGTTTAAATGTGCTATAATAAAAGATAAATT1310.10220641013637924No Hit
CTTCATGGGTACTGCGAAATGAGACTGGCCAGGGCCCCGTGGACCTGTCGGA1300.10142620853228475No Hit
GCCTACGGGAAGAGTACAAGGCCGATGCCAAATTGGTATTGCAGTTTAAAGA1300.10142620853228475No Hit
TCCTTAGGGTTATTGAGGAAAGAAAACAAGGGCTGGAGCAGTTTATAAACAA1300.10142620853228475No Hit
CAACAAGGGACAGGCCCAAGGGTCGAGAGGAGGAGAGTGATCAAATCCGGGA1300.10142620853228475No Hit
TATTACGGGTTCAGTGACACTGACAACCATTCAAGGTAGTAAACATCTAGAA1300.10142620853228475No Hit
GTAGATGGGATACCACTGAAACTTTCTCCATATGAACAATAAGGTAGTTTTG1300.10142620853228475No Hit
CACTCTGGGACCCATTCAGGGCATATCTGGAATCTGAAGTTGCTATATCTGA1300.10142620853228475No Hit
ACGACAGGGATTGCCAGAAGAAGAAACAGAGCTAGGAAATTCTAACCCAGTA1290.10064600692819026No Hit
TAAACCGGGATATCTCATTGTGGTTTTGATTTGCATTTCCCTGATGATTAGT1290.10064600692819026No Hit
AGCTTTGGGAGCCGTTTAAAATTCCTCAAAGAAGCTGGTCATGGAACACAAA1290.10064600692819026No Hit
TTGTCTGGGATCCATCAGTCAATACATGTGTGCATTACCTTAAGTTGCCTGA1290.10064600692819026No Hit
CAATCAGGGACACACACATACACAAATATTTGTAACATATTGTGACCAAATG1290.10064600692819026No Hit
TAGTTCGGGTGGCAATTCTTTAAAAAGTTTAAAATTTTGGGGCTTCGAAGGC1290.10064600692819026No Hit
TACTTTGGGGAGCACTCCCTTGGCCTGGTTGCGCTGAGGAAGGAGTTGTGGT1290.10064600692819026No Hit
GCAACTGGGATCGTCGGGGAGATGCAAATCGGAACCACAGTGAGGTATTGAC1290.10064600692819026No Hit
GTACCAGGGCCACCTCTATTTATTAATTGATTGAATTGGGTTCGCCAGGTTT1290.10064600692819026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)