FastQCFastQC Report
Sat 31 Dec 2022
SRR5844024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128443
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC4300.33477885131926227No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT3400.26470885918267245RNA PCR Primer, Index 17 (95% over 22bp)
CGTGGCGGGCGATCTGTCGGTGGGGATGCAGAGCGGAGGCTTCGCGCAGCAG1860.14481131708228553No Hit
TGCATCGGGGAGTCTCTCCGCAGCCGCGCGGCCACTGCGAGCCCAGCCGCAG1860.14481131708228553No Hit
GCATTTGGGGGGGGTTTCAGTGGCTTCTGGTGCTCTAGGGTGAGCTCTGCCC1830.14247565067773252No Hit
CGACAGGGGAGCCAAAGAGGTCGAAGGGGTTCTGGAAACTTTGGTGGTGGTC1800.14013998427317953No Hit
AAACATGGGACCTTTCTTTCTTCTTGTGAGGACATCTTGAAAAGACTATCAT1800.14013998427317953No Hit
ATGAACGGGCTTGGTCCTTGGAAGACCTATATTCTCTGTAGTTACTGAAAAC1770.13780431786862654No Hit
AGATGGGGGGCCGCGCCGGCTCTGGGCACTCAGCATCGTTTCCTTTTCCTCC1740.13546865146407355No Hit
TTAGCTGGGGTAACTGCTAATCTACATACTCTCAAGTCACTAACCTTCCTCT1700.13235442959133623No Hit
TATTACGGGTTCAGTGACACTGACAACCATTCAAGGTAGTAAACATCTAGAA1690.13157587412315191No Hit
AATTTGGGGAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCT1670.13001876318678324No Hit
AGCGCGGGGCCGATCTATAGCAGGGGAACTTGGCTCAGCTGCCGGGCTGCTC1610.12534743037767726No Hit
ACAACCGGGGATACCAGTGCTGCTGTAACTGAAGAAATGCCACCCCTTGAAG1610.12534743037767726No Hit
GCTACTGGGAGTCCTCAAAATATTTTGCACGTCAGTGTCATAAATTCCTATA1590.1237903194413086No Hit
CCAGATGGAGCACCTCTGCCCGGCCCATCATCTGGGAGGTGGGGAGCACCTC1590.1237903194413086No Hit
AGCATGGGATACTGTCGATATCATCCTTCCAAAGGGTATTGGTGGCACTTCA1580.12301176397312426No Hit
ATAGCTGGGGCCTGCAGGCACTATAATCCACCTAGACAATCAAGTCTTCATA1580.12301176397312426No Hit
AAGTACGGGATTGAACACTTACTGGAGCAAATACTTAAACTACTAGCTTGCT1580.12301176397312426No Hit
TACTTTGGGGAGCACTCCCTTGGCCTGGTTGCGCTGAGGAAGGAGTTGTGGT1570.12223320850493993No Hit
TTCACTGTGGGAACAGTCAGAGGTCGCGCAGGCGCTGGTACCCCGTTGGTCC1550.12067609756857127No Hit
CGGTCTCCTAAAAGAAAATTGTCCCGCTCACCATCCCCTAGGAGACATAAAA1550.12067609756857127No Hit
CGACTAGGGGTACAATAAGCATATGCTTTGACTTTCCTTTGCTGTGGAGGTT1540.11989754210038694No Hit
TAAGCTGGGCAGAGGACTCATGGTGATACAGCTGATGTAGCAAATGGATTTG1540.11989754210038694No Hit
ACGATAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1530.1191189866322026No Hit
ACAAATGGGATTCCACTTACAGTGCTGTCGGCGAGTATTAGAAAATCGATGG1530.1191189866322026No Hit
TAGTTCGGGTGGCAATTCTTTAAAAAGTTTAAAATTTTGGGGCTTCGAAGGC1530.1191189866322026No Hit
ACAACTGGGATTTCCTGTGCTCACCAGAGGCAAAATGTACCAATATCCTGAC1520.11834043116401827No Hit
ATTGCTGGGGATCACCACAAATCCTAAATATATTAAAAGCATAGTAAGAGAA1520.11834043116401827No Hit
TTCACTGGGAGTGGCATTACACCAAAATTTCTTAGTAGGACTCCTGCATAGA1510.11756187569583396No Hit
GTGGCTGGGCAAGCTGGTAAGGTGCTGTTCACTTGAACAGGTGCTGTTGCGC1510.11756187569583396No Hit
ATACATGGGTGGCAGCCTGAATGTTTAAATGTGCTATAATAAAAGATAAATT1510.11756187569583396No Hit
GGATCGGGGAATGCAGATATTGGAACTATTCGGAGGCGAAGGGTTACAGAAA1500.11678332022764962No Hit
CGCTAACGGGAGTGCGTTTTAGAAATTTCTTGTGACCATGTGTCACTTTGCA1490.11600476475946528No Hit
TTCGCGGGGCGCTATCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTC1490.11600476475946528No Hit
GTAGATGGGATACCACTGAAACTTTCTCCATATGAACAATAAGGTAGTTTTG1490.11600476475946528No Hit
GTAACTGGGCAAAGCCCTGTGGGACCGTGGCTTGCTCAATGGGGCCTCCCAG1480.11522620929128097No Hit
CGCTTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1480.11522620929128097No Hit
TGGCCAGGGATTCTATCTTAAAGTTTTTATATTCTCCCCACCCATCTCTTGC1480.11522620929128097No Hit
TGATATGGGGACCCAGGGAGATTTAATCTACGTGTCTTGTAGCCTGTAATTT1460.11366909835491229No Hit
CAACAAGGGACAGGCCCAAGGGTCGAGAGGAGGAGAGTGATCAAATCCGGGA1460.11366909835491229No Hit
TGCACAGGGAGATTTAAGAAGAAAACCTCTAAATTTCAAAATTATGAAGAAT1460.11366909835491229No Hit
AAACCCGGGATCCTCTTAAGCTAACTTATCTATCTAGATTAACTTGAAACTG1450.11289054288672798No Hit
TTAACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1450.11289054288672798No Hit
TTAAAAGGGATGAATAGGTGGAAATCAATAAAAATTTGCCATACCTATAGAA1450.11289054288672798No Hit
CGGCGCGGGCGATCTAGGATGGGGAACGTCACTACATGTGCTTATGCAAAAA1450.11289054288672798No Hit
ACTCGCGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1440.11211198741854364No Hit
GGATAGGGGCCATTTTAATTAAACAATGCTTCAAGGCTGCATATTAAATAGA1440.11211198741854364No Hit
GATACGGGTGTCTGAAGTTACTTGAAGGCATCACTTTTAAGAAAGCTTACAG1440.11211198741854364No Hit
AGCATAGGGAACTCCAGCACCATCTGCTCCGTTTCTTGAAGTTTGCTGAACG1420.11055487648217498No Hit
GTTTTGGGGATGTATGACCTGGATGGTGATGGCAAGATCACCCGAGTGGAGA1410.10977632101399064No Hit
ATGCCAGGGATCTCCGTGAGGGAGAGTTGAGAGAGTTAGGGCCTTGCCCTGG1410.10977632101399064No Hit
TACGCCGGGTCGCTCCGGGAGGATCATCTGTGCTTATATAACTGGGTCCCTT1400.1089977655458063No Hit
GAGTGCGGGGTATTTAAAGGCAAGATTTTGATCTTCAAAAATTTTTTTCTCC1390.10821921007762199No Hit
ACTTTAGGGATGGTTTGATTACATTTAACACTATGTAGTTGGAATTTTAAAA1390.10821921007762199No Hit
CTTCGCGGGGGAAGGGCGGCGCGCTTAGGCAGGCGGTGGCGCGGCTGGAGTG1380.10744065460943765No Hit
TCAGTAGGGCAATTACTCTTACTAGAAAGTTTGATTTTAAATCCAACCTTGA1380.10744065460943765No Hit
ACTACTAGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCC1380.10744065460943765No Hit
CTATTTGTGGGATTTATTTTTGGAATTCAAGGATGATTCAACATATGAAAAT1370.10666209914125331No Hit
GTACAGGGGGCTGATCTTCAGTGAGGTCTGCATACCTGCCATCCTTACTGAT1370.10666209914125331No Hit
AGTAGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1370.10666209914125331No Hit
ATCCATGGGCACTATGTTGCACAGTAGAATAGAAATATGAATTCAGGTACTT1370.10666209914125331No Hit
GTACCCGGGAAGATGTCTCAGGAGCCATATCACATCTGTCCCTTGGGGAGAT1370.10666209914125331No Hit
CGAGAGGGGGAAAAAAGTGGAGAAGTCTGTTAATGAAGTGATGGAATGGATG1370.10666209914125331No Hit
TTATCGCGGGAACATTGGTTTGGCTATCTTCTAGTTTGGTGGCATTAACCAA1370.10666209914125331No Hit
TTATGCGGGGGGGGACCAAGGAGAAACTTTTACCCAAAGCCCTATTACCTCC1360.105883543673069No Hit
TCCTTAGGGTTATTGAGGAAAGAAAACAAGGGCTGGAGCAGTTTATAAACAA1360.105883543673069No Hit
TACGTTGGGGAGAGGGTTGCGGGCTAATCCAAAAGAATTGGTTTATAATTAT1360.105883543673069No Hit
TCATCTGGTCTATGCAGGGGCTTCCGATCTTGGTGGGGAGTGGAAATGTTTG1360.105883543673069No Hit
TGAGATGGGAGCTGTTTTTGGTACCATTTTAATTTTTGCAACTTACTTGTAA1350.10510498820488466No Hit
TTACATGGGTGATGAAATGAAATAATGAAAATGAAATGGAAATGAGATGAGA1350.10510498820488466No Hit
TCCTCAGGGGATCTGTAAGCGGGACCCCAAAACTTCAATCTGAAATGTCTTA1350.10510498820488466No Hit
CTCGCTGGGGTGCCACCGCAGGCAGCGCTCCAGTCGGGAATGGCCAGGATGG1350.10510498820488466No Hit
ACAACTGGGAGTGGCGGCAGCGGCGGCGACGGCAGTAACAGCGGCAGCTACA1350.10510498820488466No Hit
AAGCCCGGGGGGCACTCTATCAGTCTATAACTATCAACGTAGTGCAACAGGG1350.10510498820488466No Hit
ACATCAGGGAGTCATCCCTTGACTTTTCAAATGACAGAATTTTTTCCAATTG1340.10432643273670032No Hit
TGAAATGGGAGAGGTTATGTCATTTTATAAAGACTTCATTGATAAGATGGCT1340.10432643273670032No Hit
CTAATAGGGTAAATGATTTCTTAAGTATTAAAAACCTTTTAAAGAACAGAAA1330.103547877268516No Hit
ACGGATGGGACAAATATCCATCTCAGAGTCAGCTTCCCAAGGGACCCACTGG1330.103547877268516No Hit
TAAAGCTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG1330.103547877268516No Hit
AACCTGGGGATGAAATGGAAGAAAAGGATATACTAGCTGTAGAAGATATGAG1330.103547877268516No Hit
TATTGTGGGAAGAATTCAGAGAGAAGCTGCTAGAATACTTTGTGCATTTCCT1320.10276932180033167No Hit
ACAAGCGGGTGAAGCAGAAATTTAAAAAATGATATTGGGACCTGGAAGTTTT1320.10276932180033167No Hit
TCTCAGGGGGCGCTTCCCAGAGTGAAATTGCTCGGGCTGGTGCGTCTGGGCG1320.10276932180033167No Hit
TGCATGGGGAGGTGTTGGCATTCTTCGCTGATTTGGCTGTTCCCAATGTTTA1320.10276932180033167No Hit
GGCAAGGGAGGTGAAAGAATAGAGTTTTATATCCAATTGAAGTTAAAGTTGT1320.10276932180033167No Hit
TGTACGGGATTAAACAGATCGCATATTATGATCTTGCTGCAGCCACAGTGCA1320.10276932180033167No Hit
TGTCAGGGGTGAGTTTACACTAAATCTCAGACTATGGATCTTCAGGAAATGA1320.10276932180033167No Hit
ATCCGCGGGTATTTTGAAAGTGTAATTCCCCTCTGACACAATATCCCTCACA1310.10199076633214733No Hit
GCTGCTGTATTTATACAAGTAAAGGGCCGGACCTCTCGCTTCTTAAGTTATT1310.10199076633214733No Hit
GTTCAAGAGGGTAATGAAGAATCTTAGGCGGGTGCACCCAGTTTCCACCATG1310.10199076633214733No Hit
GTCATTGGGCGGTAATAAAAATGAGCATTTCAAATGTCCTGTAGACCTAAAG1300.10121221086396301No Hit
CATAAAGGGGGACAGACTGCGGGACGGACGGTGGACGCTGGGACGCGTTTGT1300.10121221086396301No Hit
TTGTTGGGGGAAAAGGACCCCACGAAGTGTTGGATATAAGCCAGACTTTGTT1300.10121221086396301No Hit
CTCTCGGGGCAGCAATGGTTCTTAAACAGGTTGAAATGACAGAAATGGAATT1290.10043365539577867No Hit
ACCAAGGGGAACAAGCACCTGGAAGTGCGGCACCAGCGGAGCGGGCGTGGGG1290.10043365539577867No Hit
GACCGTGGGATATATTCTGGACATTAGTTCTTCATCAGATGTATGACTTAAT1290.10043365539577867No Hit
TAACGCGGGGTGTCACACAGGGACAGCCGCAATCCGGACCTGGCGGTGTATG1290.10043365539577867No Hit
TAGTTAGGGAGCCCAGCACACCAACCCATGCTGCTGGCGTCTAAGTGTTTGG1290.10043365539577867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)