FastQCFastQC Report
Sat 31 Dec 2022
SRR5844023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128765
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC4130.32073933134003807No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT3420.26560012425736806RNA PCR Primer, Index 17 (95% over 22bp)
CGTGGCGGGCGATCTGTCGGTGGGGATGCAGAGCGGAGGCTTCGCGCAGCAG1750.1359064963305246No Hit
GCATTTGGGGGGGGTTTCAGTGGCTTCTGGTGCTCTAGGGTGAGCTCTGCCC1730.13435327922960433No Hit
TGAGATGGGAGCTGTTTTTGGTACCATTTTAATTTTTGCAACTTACTTGTAA1670.12969362792684347No Hit
CCAGATGGAGCACCTCTGCCCGGCCCATCATCTGGGAGGTGGGGAGCACCTC1670.12969362792684347No Hit
TAACTGGGGGGTCTGGAGTGGACCTCCAGCAAACTCCAGCAGACCTGCAGCA1610.12503397662408264No Hit
GTTTTGGGGATGTATGACCTGGATGGTGATGGCAAGATCACCCGAGTGGAGA1610.12503397662408264No Hit
TGTACGGGATTAAACAGATCGCATATTATGATCTTGCTGCAGCCACAGTGCA1610.12503397662408264No Hit
TATTACGGGTTCAGTGACACTGACAACCATTCAAGGTAGTAAACATCTAGAA1600.12425736807362249No Hit
ACTCGCGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCG1590.12348075952316234No Hit
TTATGCGGGGGGGGACCAAGGAGAAACTTTTACCCAAAGCCCTATTACCTCC1580.1227041509727022No Hit
CGGTCTCCTAAAAGAAAATTGTCCCGCTCACCATCCCCTAGGAGACATAAAA1570.12192754242224207No Hit
CCTTCCGGGATATACACATGATTTCATATGAAAGTAATATTTGCTGTAGAAA1560.12115093387178194No Hit
TTCGCGGGGCGCTATCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTC1560.12115093387178194No Hit
CGCTTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1560.12115093387178194No Hit
ATGAACGGGCTTGGTCCTTGGAAGACCTATATTCTCTGTAGTTACTGAAAAC1560.12115093387178194No Hit
AAGTACGGGATTGAACACTTACTGGAGCAAATACTTAAACTACTAGCTTGCT1560.12115093387178194No Hit
GCCTACGGGAAGAGTACAAGGCCGATGCCAAATTGGTATTGCAGTTTAAAGA1540.11959771677086165No Hit
TCCTCAGGGGATCTGTAAGCGGGACCCCAAAACTTCAATCTGAAATGTCTTA1540.11959771677086165No Hit
AGATGGGGGGCCGCGCCGGCTCTGGGCACTCAGCATCGTTTCCTTTTCCTCC1540.11959771677086165No Hit
TACGTTGGGGAGAGGGTTGCGGGCTAATCCAAAAGAATTGGTTTATAATTAT1540.11959771677086165No Hit
TTAACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1530.11882110822040151No Hit
TGCATGGGGAGGTGTTGGCATTCTTCGCTGATTTGGCTGTTCCCAATGTTTA1520.11804449966994136No Hit
AAACATGGGACCTTTCTTTCTTCTTGTGAGGACATCTTGAAAAGACTATCAT1520.11804449966994136No Hit
AATTTGGGGAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCT1470.11416145691764065No Hit
CTCGCTGGGGTGCCACCGCAGGCAGCGCTCCAGTCGGGAATGGCCAGGATGG1450.11260823981672038No Hit
GCTACTGGGAGTCCTCAAAATATTTTGCACGTCAGTGTCATAAATTCCTATA1440.11183163126626025No Hit
TACTTTGGGGAGCACTCCCTTGGCCTGGTTGCGCTGAGGAAGGAGTTGTGGT1440.11183163126626025No Hit
ACAACTGGGATTTCCTGTGCTCACCAGAGGCAAAATGTACCAATATCCTGAC1430.1110550227158001No Hit
GTCATTGGGCGGTAATAAAAATGAGCATTTCAAATGTCCTGTAGACCTAAAG1430.1110550227158001No Hit
GATACGGGTGTCTGAAGTTACTTGAAGGCATCACTTTTAAGAAAGCTTACAG1430.1110550227158001No Hit
ATCCATGGGCACTATGTTGCACAGTAGAATAGAAATATGAATTCAGGTACTT1430.1110550227158001No Hit
TTAGCTGGGGTAACTGCTAATCTACATACTCTCAAGTCACTAACCTTCCTCT1430.1110550227158001No Hit
CGAGAGGGGGAAAAAAGTGGAGAAGTCTGTTAATGAAGTGATGGAATGGATG1430.1110550227158001No Hit
TAAATAGGGGAAAAGGAGAATCTTATGAATGCAGAGAACCTTGGAATCGTCT1420.11027841416533996No Hit
ATGATTGGGAGGGTTGCAAAAGATGGGCTTTGTAGGTCATTTGCATCTTGGT1420.11027841416533996No Hit
AATTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1410.10950180561487982No Hit
ACATCAGGGAGTCATCCCTTGACTTTTCAAATGACAGAATTTTTTCCAATTG1410.10950180561487982No Hit
AGTAGAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1400.10872519706441967No Hit
TGCTCTGGGTACGTTATCTTCAGTAGTGTGTTTTTGGTTGGTTAAATTTGAC1400.10872519706441967No Hit
TTGTCTGGGATCCATCAGTCAATACATGTGTGCATTACCTTAAGTTGCCTGA1400.10872519706441967No Hit
TGCACAGGGAGATTTAAGAAGAAAACCTCTAAATTTCAAAATTATGAAGAAT1400.10872519706441967No Hit
TTAAAAGGGATGAATAGGTGGAAATCAATAAAAATTTGCCATACCTATAGAA1390.10794858851395954No Hit
CGGCGCGGGCGATCTAGGATGGGGAACGTCACTACATGTGCTTATGCAAAAA1390.10794858851395954No Hit
ATACATGGGTGGCAGCCTGAATGTTTAAATGTGCTATAATAAAAGATAAATT1390.10794858851395954No Hit
TAAAGCTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG1380.10717197996349939No Hit
TTTACAGGGAGACTTATGAGAAATCCAAGATTTCCAATGCAAATTTAATTCA1370.10639537141303924No Hit
TGCATCGGGGAGTCTCTCCGCAGCCGCGCGGCCACTGCGAGCCCAGCCGCAG1370.10639537141303924No Hit
ATAGTTGGGTAATTACAAAAATCTAGGTATGTCTTTACTGAAGAACTATCAT1370.10639537141303924No Hit
ACTACTAGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCC1360.10561876286257912No Hit
TGGCCAGGGATTCTATCTTAAAGTTTTTATATTCTCCCCACCCATCTCTTGC1360.10561876286257912No Hit
TGTCAGGGGTGAGTTTACACTAAATCTCAGACTATGGATCTTCAGGAAATGA1360.10561876286257912No Hit
GGGAGAGTGGGTCTGGAACAAATACTACTTGGTCATTGTGAAATAATGATTT1350.10484215431211899No Hit
GAGTGCGGGGTATTTAAAGGCAAGATTTTGATCTTCAAAAATTTTTTTCTCC1340.10406554576165884No Hit
TCATCTGGTCTATGCAGGGGCTTCCGATCTTGGTGGGGAGTGGAAATGTTTG1340.10406554576165884No Hit
AAGCCCGGGGGGCACTCTATCAGTCTATAACTATCAACGTAGTGCAACAGGG1340.10406554576165884No Hit
AGCGCGGGGCCGATCTATAGCAGGGGAACTTGGCTCAGCTGCCGGGCTGCTC1330.1032889372111987No Hit
ACGCTTGGGAGTGCAGGCTGAGGATGGCCTTGGGACTGGAAAAGCCACTCTT1330.1032889372111987No Hit
TCAGTAGGGCAATTACTCTTACTAGAAAGTTTGATTTTAAATCCAACCTTGA1330.1032889372111987No Hit
TTAGAGTGGGGTGTTCTTTCCCTTTATCTATGTGTTGTCTCTGAGGAAATGT1330.1032889372111987No Hit
CGACAGGGGAGCCAAAGAGGTCGAAGGGGTTCTGGAAACTTTGGTGGTGGTC1330.1032889372111987No Hit
GGCAAGGGAGGTGAAAGAATAGAGTTTTATATCCAATTGAAGTTAAAGTTGT1330.1032889372111987No Hit
ATAGCTGGGGCCTGCAGGCACTATAATCCACCTAGACAATCAAGTCTTCATA1330.1032889372111987No Hit
AACCTGGGGATGAAATGGAAGAAAAGGATATACTAGCTGTAGAAGATATGAG1330.1032889372111987No Hit
CACAATGGGGCTCTCTTTGCATCAGTCACGCTGTGTCCTCCTCACACCAGAG1320.10251232866073856No Hit
TAAGCTGGGCAGAGGACTCATGGTGATACAGCTGATGTAGCAAATGGATTTG1320.10251232866073856No Hit
CGCTAACGGGAGTGCGTTTTAGAAATTTCTTGTGACCATGTGTCACTTTGCA1310.10173572011027841No Hit
GGATAGGGGCCATTTTAATTAAACAATGCTTCAAGGCTGCATATTAAATAGA1310.10173572011027841No Hit
TTTATGGGGCTTCGTACTTTCTATGAGAAGCGTATGGCCACAGAAGTTGCTG1310.10173572011027841No Hit
GTTCAAGAGGGTAATGAAGAATCTTAGGCGGGTGCACCCAGTTTCCACCATG1300.10095911155981827No Hit
TCCTTAGGGTTATTGAGGAAAGAAAACAAGGGCTGGAGCAGTTTATAAACAA1290.10018250300935813No Hit
CTGTAGGGGGTGCCCACCCCTCAAGTGTAAGGAACTGCGTTCCGCCCCTCAG1290.10018250300935813No Hit
GTTGACGGGTGAAAGGAAATTAAGAAAGCAATCCCATTTACAATACTATCAA1290.10018250300935813No Hit
ACTAGGGGGTGGACAACGGTGCTTCCCCAACAGCTGATACCGCCGTCCTGGG1290.10018250300935813No Hit
GTACCAGGGCCACCTCTATTTATTAATTGATTGAATTGGGTTCGCCAGGTTT1290.10018250300935813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)