FastQCFastQC Report
Sat 31 Dec 2022
SRR5844022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29205
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAATGGGAGGCCGTCCGGGCGGCGCAGTCCCTCGGATCGCTCTCTCCTCA2100.7190549563430919No Hit
GTATCGGGGGATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGG1930.6608457455915083No Hit
GTGTTAGGGCCGGCCTCTCCCTGTACACGTGCGGGAGGAAGGTGTGGTGCTG1880.6437253894881013No Hit
CTGTTGGGGGCTCTATCTCCATTTATGTTACATCTTCTGCCAAATAGCTTTG1860.6368772470467385No Hit
CGCATAGGGCTGTGTGATTTATTTCACTTGGTTATATTTATTTCTGATGGTT1820.6231809621640131No Hit
ATATATGGACAGGTGTGAGCCACCATGCCCAGCCAGATCTTTCTTGTTTTTG1800.6163328197226503No Hit
TAGCGGGAGGCAGGAACACTGGAGCCTGCAATAAGGGAGGAGAGGGGACTGG1790.6129087485019689No Hit
TTGGCTGGCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCA1750.5992124636192433No Hit
AGATATGGAGGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAAGGGACGAG1730.5923643211778805No Hit
GCGGGTGGGGGAAAAAAGGTCCCGGAGAGCTGAGCAGTCAAGATGTGTGACT1730.5923643211778805No Hit
GACAAGGGGGCAGGGTCTCCTTGGTATGCCTACACACACTTTACAGGGAACT1720.5889402499571991No Hit
TAAAATGGGGAGTTATTATTTTCCAAGTTTGAACATGTTAAATATCAGATGC1720.5889402499571991No Hit
ACATTAGGGCATTTTTCCCCGCTTAGTTTGTTGACTCCGTGTTCATCAGCAT1630.5581236089710666No Hit
GGGCTGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1590.544427324088341No Hit
TTCTGGGGGGGGAGCGGCCAGTGGACTCTCGCCGCCACCCGGTCCAGGTGCC1540.527306967984934No Hit
ATCATGGGCCAATGTGTTTGCCATGTTTGACCAGTCACAGATTCAGGAGTTC1540.527306967984934No Hit
TGCTGCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1540.527306967984934No Hit
TTCCCGAGGGGGTCTCTTCTATTATCGCCTCCTCTTAGTTGGCATTGATGAA1530.5238828967642527No Hit
TGATATGGGATTGCACTGTTTCTCCACTTTTTATTTTCTCTTAGGCTTTTGT1520.5204588255435713No Hit
TCTTATGGGCAGGTGTTCATACAGTGGCATTTGGGACACAATCGTTGGAACC1520.5204588255435713No Hit
TAAAATGGGCTTTGGTATCGTGGAAGGACTCATGACCACAGTCCATGCCATC1520.5204588255435713No Hit
TAAGTGGGGTCCGATCTTGCTGTGGGAACCTGGCGCCGTTGGCATCGTAGAG1490.5101866118815271No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC1480.5067625406608457No Hit
TAGACAGGGCTCTTCGTTAAGTCGGCCTTCCCAACATGGCGCAGTCTATTAA1480.5067625406608457No Hit
ACCACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1460.499914398219483No Hit
GAACTAGGGATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACTTAT1460.499914398219483No Hit
GGATACGGGGAGTCTCGCTCTGTCACCCGGGCTGGAGTGTAACGTGGCATGA1440.4930662557781202No Hit
CTTAATGGGCGCGGCGGCGATGTCCGCGAGCCAGGCGAGTGCCGCGGCGGCA1420.4862181133367574No Hit
AGCTTTGGGAGTGTCTCCGCGGCGCACCTCGCGGCGAGAATCCGGAGGAGAA1420.4862181133367574No Hit
TATCACGGATAGTCACAATGGCAAAGGGAGAACTGGGGGGCTGGCAGGTAAT1410.482794042116076No Hit
AAGCACGGGGTATGGTACCACTGTGTTGGTTATATGGTGAATCTGAACGTAC1410.482794042116076No Hit
TTCCAAGGGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATG1410.482794042116076No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAG1410.482794042116076No Hit
GAGTGCGGTCGCGCTGGGGATCCACTGTTAAAGAACTGCATAGGTGATTTCC1400.4793699708953946No Hit
TCAGTGGGGAATGACTGACAGAATATTTTGTTGGGCAGTCCTGATTTAAAAC1400.4793699708953946No Hit
AGCCTAGGGTGCCACCACTGCCCTGCTCCACTATCAGCTGCCCCAGATGCCG1390.47594589967471324No Hit
TTAAGTGGGAAGAAGGCACTTGTTTGTAAGCCAGAGAAGAAACTTTAATTGC1390.47594589967471324No Hit
GAAGTGGGGACTGTCGGACAGAAGCCGGTTTCTCTGGGATCCAAGACGTGTA1380.4725218284540318No Hit
AGTAATGGGTCCTTCAGGATATCAAGAAACCAGACTGTGATGACTGGGAGAG1380.4725218284540318No Hit
GCATACGGGAGAGACAATTATGACAACTGAAATGAGACATGCACATAATATA1370.4690977572333504No Hit
TACTAAGGGACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAA1360.465673686012669No Hit
TGCGCAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1330.4554014723506249No Hit
GAAAGTGGGAAGCTGAATGGTATCTATCACTTATTTATCCTTGACAGGTTAT1330.4554014723506249No Hit
TGCTTAGGGAGACTCTCAAAATCGGAAGAAGCTTTCAGAGCTGTTAAGGTAC1320.4519774011299435No Hit
CTATCTGGGTGGTCTGGCAACTACTTCACGGAGACGGCAGGCACCTCGCTGC1320.4519774011299435No Hit
CAGAAAGGGTGTCACTGGGATCACCTTCCTGTCTGGAGGCCAGAGTGAGGAG1320.4519774011299435No Hit
CGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTC1300.4451292586885807No Hit
TAAGTAGGGGTTATTAGTTAGCAAAATGAACCTGGGGAAGTGGGAAGAGCTA1300.4451292586885807No Hit
CCAACAGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA1300.4451292586885807No Hit
TTTACAGGGATTCTGGAAGAGGAGGAAGAAATTGATAATACAGTTCCTCCTG1260.43143297380585516No Hit
CTGCGCCGGGTCTGGAAGCTTTCTAGCTTTACCGCTTCTATTGATCCACACT1250.4280089025851737No Hit
CTTTTGGGTCCAATATCCACCTTCCCAAACCTCATGGAAGCACAAGAATCCT1240.4245848313644923No Hit
GATGATGGGCTGTCCCACAAATAGTTTTTTGTTTACGATTTATGACAGGTTT1240.4245848313644923No Hit
TGAGGTGGGCAATTGAAAAAATTAACAATGTTTTATTTTTGTGAAATTTTTG1240.4245848313644923No Hit
CATTGAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1220.41773668892312965No Hit
CTAGGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1200.4108885464817668No Hit
GCCAAGGAGTCTTTTTAAAAATAATTTCCTTTTACAATTCCCTTTGTCTTTA1200.4108885464817668No Hit
GCGCGAGGGGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGT1190.4074644752610854No Hit
TGAACAGGGCTTTTCAGTCCTTGCGCACCGGGGAACAAGGTCGTGAAAAAAA1160.39719226159904125No Hit
CCAATAGGGAAGTGCTGGAATTTTCCAAAACAGCCTGTTCTCTCCTCTCTCC1150.39376819037835986No Hit
TAAAGCGGGGGTTAGGAAGGAAGACGTGAGTTTAGGAGACACATGTAGAGCC1150.39376819037835986No Hit
AACTTTGGGGCCCTGTGACCGGCTTGGCGGTTGGAGCGGCTGTCGCCATGTT1150.39376819037835986No Hit
TCACTAGGGGACGGCGCAGGGGCTGCGGGGAGCGCCGCGCAGGCCGTGCAGT1150.39376819037835986No Hit
ATGTCGGGGGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT1140.39034411915767847No Hit
GTGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1130.3869200479369971No Hit
CGAACTGGGGACGCTCTTCCGATCTTTTGCCGGGAGATGCGCCACGGTTTCG1100.3766478342749529No Hit
TAATATGGGAGAGCAGTACCTTTCCAGATTCGAAGAAGCAATGAAGCTAAGG1090.3732237630542716No Hit
ATCGCAGGGAGTCCTTGTGACCTAATCATCTCCCAGAAGGCTCCGCCTCCCA1090.3732237630542716No Hit
TCTAGAGGGATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAA1090.3732237630542716No Hit
CTAGAGGGGCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTT1080.3697996918335901No Hit
TTTCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1070.36637562061290874No Hit
AGCATAGGGTATACAGCGGAGTCTTCAGATACACTGTGTCCTCGATGTGCAG1050.35952747817154596No Hit
TACCCAGGGGTACTCGTCATCGGCCATTGCACTGGGGAACTCCCTCACCCCA1050.35952747817154596No Hit
TGGAACGGGAGGTAGGAGGTCCTGGGTGACTTTGGAAGTCCGTAGTGTCTCA1050.35952747817154596No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT1020.3492552645095018RNA PCR Primer, Index 17 (95% over 22bp)
GTAAGATGGGGAGGCTACAGTTGATATTTAAAAGATCCATTTAAAACAAGCT1020.3492552645095018No Hit
TGTTATGGGACGTCCCACAAATGCAGGTGTCCTGAGCACCACACCCAGTGGG1020.3492552645095018No Hit
CATTAGGGGAGGAGGAGGAGGGACTCGGCGGGAGGATGGGCTTGCTCTCAAT1010.3458311932888204No Hit
TCAGCAAGGGCTTCTGGCTTTTCCCTTGAGGATCCCCAGACCCACTCCAACC990.3389830508474576No Hit
AGCAATGGGATGGACAAGAAGCCAACTTAGAAAGAAGGGTCTCACGTGGCTG960.3287108371854135No Hit
TATCAAAGGGTGCTTCTGTTTTTTCACCATCATTTGGTCTGAAACAGACAGA960.3287108371854135No Hit
TTTTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC960.3287108371854135No Hit
TTGTAAGGAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGG950.32528676596473205No Hit
AGCAACGGGGCTGACTTACAGACACAGGCTGTTTACAAAATGCTAGCTGGAA950.32528676596473205No Hit
TCGTCAGGGGTTGTCTCAGGATTGGAGGGTGAAAGGGGCATTGTTATAGGGT950.32528676596473205No Hit
TAACGCGGGGGAGCCGCCGCCATGTCTGCGCATCTGCAATGGATGGTCGTGC950.32528676596473205No Hit
TGTGTGGGGGGAGCGGCGGAGGCGCCCAGCGGAGGTGAAAGTATTGGCGGAA920.3150145523026879No Hit
GTAGGCGGGACCTTTGTGCACAAATAACATTATATATATTATATATCTATTC920.3150145523026879No Hit
CGGTGAGGGACGGTGACCATTTTTTTGTGTGTACAGCGTCATATAGGCTTTG910.31159048108200654No Hit
TCATGTGGGATAAATTCAATGGAATTTGAGAAGCAATGGCAGCGGGATAATT890.3047423386406437No Hit
CTTTGGGGGATCCATTTTGAAGAGACGGGGGAGCGGGGGGCTCGTCTGTTCC890.3047423386406437No Hit
GTTAACGGGCCGATCTGTTCAAGGGGGATCCGTGGTGTGCCGCTACTGCCGG880.3013182674199623No Hit
CCTCAGGGGTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCC880.3013182674199623No Hit
TTTCGCGGGCTAACAATTATATGGTAAATGAATAAGCTCGTTATTTGAAAAT880.3013182674199623No Hit
CATCGCGGGAAGGGCTTCCCAGGTGGTGCTGGACCCTGACGACAGCCATTTG870.2978941961992809No Hit
GCTACAGGGATACCAACTGGGCTATTGGATTAAGTGTGGCTGATCTTATTGA860.29447012497859953No Hit
TTGTATGGGGGACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCGTTCTGAGC840.2876219825372368No Hit
CTATGCGGGTAGTGTGGCATTGCAGTGATGGCTGTTTAATAAAGGTCCATCG840.2876219825372368No Hit
TTGAATGGGTATCTGAATGATAAAGGCCTAAGTGATAACTCTGGACAAGGGA820.28077384009587397No Hit
CACCCAAGGGAGAGCCTGTTCTTCGAGACATCGTCCATTTCGTGCCCTTCAG800.2739256976545112No Hit
GGTTTGGGGGGGAAGGGATCTATGGGAAATCTAGGCGAAAGTAATGGGGAAG780.26707755521314847No Hit
TAATCCGGGGGGTTGATTACACAATGCCTTGTACATGATATAGAGGCATCAA780.26707755521314847No Hit
CGATGGGGATTCGCTTGACTCACGCCTTCGCCGTAGCATCTTTCGCAGCGGA780.26707755521314847No Hit
ATGTCAAGGACTGTGGCGAGTTTGATAATGCTGGTGGAATGATGAGTGTGGA740.25338127033042285No Hit
TTGGTTGGGTTCAAAGTTCTGGGGCTTTGGGCGGAAAACAGCCAGCTTTGGC740.25338127033042285No Hit
GCGCCAGGGATTCTTGTATGGCTTTGGTAGGCATACTTGGAGAACATATCCC740.25338127033042285No Hit
GTAATGGGGATTCTCATAGGAAATAAAGCAGATTTGGAGGCACAGAGAGATG730.2499571991097415No Hit
ATGCTTGGGTATATTTGCTTTTTTTCTCACCTGCACTTAGAGGAAATTTGAA720.2465331278890601No Hit
CACCGAGGGGCTTTTATGTACTGACTTCATAAAAGCCATCTCAGAGCCATTG710.2431090566683787No Hit
CACATAGGGGGGCCGGGGGGTGCGGGCGCCCTCGCCGCCCGGCTACCTACCG700.2396849854476973No Hit
GCAATTGGGAATGGAGAAACCCTAGAAAAGATCACCAACAGCCGTCCTCCCT680.2328368430063345No Hit
AAGTAAGGGGCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGT680.2328368430063345No Hit
TGCCATGGGGCTTCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAG680.2328368430063345No Hit
GACTCAGGGACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAG640.21914055812360897No Hit
AAATATGGGGATCTGTTTTGGATGTTGCTATAGTGACATGCAGTTATATATT640.21914055812360897No Hit
CTTCCTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA640.21914055812360897No Hit
TCTTGTGGGATAGAATGTGTGAAACACTGTATTAGTAAACTTCATCACCTTT640.21914055812360897No Hit
GTGGGTGGGAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACC630.21571648690292758No Hit
GCAAAAGGGAAGTTGATCCTTTTACTTACTTATGTAGAAGAGGCACATGGTA590.20202020202020202No Hit
TTACGCGGGGGAACGCACTCACAGTCGCATCATAATCCTCTCTCAAGGACTT580.19859613079952063No Hit
GCCGCCGGGACAGGCTCCTGGAGGGCTGCCTGGGGGAGGCAGACATGGGAGT580.19859613079952063No Hit
CTGTAAGGGATCTCGGCTCACTGCAAGCTCCGCCTCCCGGGTTCATGCCATT570.19517205957883924No Hit
CCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACT570.19517205957883924No Hit
GCACATGGGGGGGCTGGTCCCGCAGGCGCTCGGGGTTGGAGCCAGCGACCGT560.19174798835815784No Hit
GTCGTTGGGATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCATTCTCATAA550.18832391713747645No Hit
GGCTCAGGGATATACCTAATGCTAAATGACGAGTTGATGGGTGCAGCACACC540.18489984591679506No Hit
CTAGTGGGGAGAACCCTGGTGAGGAAGCTCCAGTCCTGCTCTCTACCCAGCC520.17805170347543228No Hit
TCAAGAGGGAAGAATGTGTGTCCAATACGTGATCAAGTAGTTTAATATGGTA520.17805170347543228No Hit
TTACATGGGTGTCATGAGGTCACTGGGTCAGAACCCAACAGAAGCTGAATTG500.1712035610340695No Hit
TGTACAGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCT500.1712035610340695No Hit
TTATTCAGGGGAAGTATATACTACCTATGAGTCCAATTAACATGAGTATTAT490.1677794898133881No Hit
ACAACAGGGCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTT480.16435541859270675No Hit
TTTGTCTGGGAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCG480.16435541859270675No Hit
CATAAGGGCTGGTAAGGTGCTGTTCACTTGAACAGGTGCTGTTGCGCAGAAA480.16435541859270675No Hit
AAGATAGGGAAGCGCAAAAGAAGAAAGATGAGGCAGAGGTCCAAGTAAACCG480.16435541859270675No Hit
GCAGAAGTATAAAAGAATAAAGAAACCTGAAGGAAAGCCAGATCAGAAGTTT460.15750727615134394No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA450.15408320493066258No Hit
TGATCAGGGATGGTCTTGTTTGTTGACATGACAAATTAACATTCTTATAGTT450.15408320493066258No Hit
AGCCAAGATACTGATATTTTAAAACAAAATGGAAAAATGTCCAATACAATAT450.15408320493066258No Hit
GTATGTGGGCAATATCTGTCACCTAGTAAGCAACCAAGAGACCCTCCCTTCA420.1438109912686184No Hit
GCGCGGGGGTCTTCCGATCTACACGTGGGCGAAGGAAGATTATGTCTTCCCC410.14038692004793699No Hit
CACAGCGGGTCGTATACCTGAGGTGCACCGGAGGTGAAGTCGGTGCCACTTC410.14038692004793699No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAG390.13353877760657423No Hit
TGTGCTGGGAGGCTGCTGGGGCCTCAGACGAGACCCAAGGGCAGAGCATTTA380.13011470638589281No Hit
AGTCTAGGGCCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG370.12669063516521142No Hit
ACTCTTGGGCATTTATTTGTGTCTTCTTCAATTTATTTCATCAATGTTTTAC350.11984249272384866No Hit
TTGATTGGGGGGCCCCCGCGGACCCAGGTGCTCTGGGTCACGGTCCCTGTCC320.10957027906180448No Hit
ACTTAAGGGGGACTGTCATTTTGGCATCTGCAAAGGAATCACTTTAGAAAGC320.10957027906180448No Hit
ATCGTTGGGGCTATGTTTTGGAGAGAGCAATCTTGCTGTGAAACAGTGTGGA310.10614620784112308No Hit
TAGTAGGGGGCTATATTGCAATTGGACTGGAGGCTATAAGTGGTCTACGGCT300.1027221366204417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)