FastQCFastQC Report
Sat 31 Dec 2022
SRR5844021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28917
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTGGGGGCTCTATCTCCATTTATGTTACATCTTCTGCCAAATAGCTTTG2190.7573399730262476No Hit
AGATATGGAGGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAAGGGACGAG2170.7504236262406196No Hit
GGCAATGGGAGGCCGTCCGGGCGGCGCAGTCCCTCGGATCGCTCTCTCCTCA2000.6916346785627832No Hit
GTATCGGGGGATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGG1930.6674274648130857No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC1870.6466784244562023No Hit
TTGGCTGGCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCA1860.6432202510633883No Hit
GTGTTAGGGCCGGCCTCTCCCTGTACACGTGCGGGAGGAAGGTGTGGTGCTG1860.6432202510633883No Hit
GACAAGGGGGCAGGGTCTCCTTGGTATGCCTACACACACTTTACAGGGAACT1750.6051803437424352No Hit
TCAGTGGGGAATGACTGACAGAATATTTTGTTGGGCAGTCCTGATTTAAAAC1670.5775149565999239No Hit
TTCTGGGGGGGGAGCGGCCAGTGGACTCTCGCCGCCACCCGGTCCAGGTGCC1660.57405678320711No Hit
TGCGCAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1650.570598609814296No Hit
TAGCGGGAGGCAGGAACACTGGAGCCTGCAATAAGGGAGGAGAGGGGACTGG1650.570598609814296No Hit
TAAAATGGGCTTTGGTATCGTGGAAGGACTCATGACCACAGTCCATGCCATC1650.570598609814296No Hit
GGGCTGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1590.5498495694574126No Hit
TGCTTAGGGAGACTCTCAAAATCGGAAGAAGCTTTCAGAGCTGTTAAGGTAC1570.5429332226717848No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAG1550.536016875886157No Hit
ACATTAGGGCATTTTTCCCCGCTTAGTTTGTTGACTCCGTGTTCATCAGCAT1530.5291005291005291No Hit
TGCTGCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1520.5256423557077151No Hit
CGCATAGGGCTGTGTGATTTATTTCACTTGGTTATATTTATTTCTGATGGTT1520.5256423557077151No Hit
ATATATGGACAGGTGTGAGCCACCATGCCCAGCCAGATCTTTCTTGTTTTTG1510.5221841823149013No Hit
ATCATGGGCCAATGTGTTTGCCATGTTTGACCAGTCACAGATTCAGGAGTTC1500.5187260089220874No Hit
GCATACGGGAGAGACAATTATGACAACTGAAATGAGACATGCACATAATATA1450.5014351419580179No Hit
GGATACGGGGAGTCTCGCTCTGTCACCCGGGCTGGAGTGTAACGTGGCATGA1410.4876024483867621No Hit
TAAAATGGGGAGTTATTATTTTCCAAGTTTGAACATGTTAAATATCAGATGC1400.4841442749939482No Hit
TTCCAAGGGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATG1400.4841442749939482No Hit
CATTAGGGGAGGAGGAGGAGGGACTCGGCGGGAGGATGGGCTTGCTCTCAAT1390.48068610160113434No Hit
CCAACAGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA1380.4772279282083204No Hit
TACTAAGGGACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAA1370.4737697548155065No Hit
TGATATGGGATTGCACTGTTTCTCCACTTTTTATTTTCTCTTAGGCTTTTGT1370.4737697548155065No Hit
CTAGAGGGGCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTT1370.4737697548155065No Hit
CATTGAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1350.4668534080298786No Hit
TCTTATGGGCAGGTGTTCATACAGTGGCATTTGGGACACAATCGTTGGAACC1350.4668534080298786No Hit
TAAGTGGGGTCCGATCTTGCTGTGGGAACCTGGCGCCGTTGGCATCGTAGAG1350.4668534080298786No Hit
TTAAGTGGGAAGAAGGCACTTGTTTGTAAGCCAGAGAAGAAACTTTAATTGC1340.46339523463706467No Hit
TATCACGGATAGTCACAATGGCAAAGGGAGAACTGGGGGGCTGGCAGGTAAT1330.45993706124425077No Hit
GAAAGTGGGAAGCTGAATGGTATCTATCACTTATTTATCCTTGACAGGTTAT1330.45993706124425077No Hit
AGCAACGGGGCTGACTTACAGACACAGGCTGTTTACAAAATGCTAGCTGGAA1330.45993706124425077No Hit
GCGGGTGGGGGAAAAAAGGTCCCGGAGAGCTGAGCAGTCAAGATGTGTGACT1330.45993706124425077No Hit
AACTTTGGGGCCCTGTGACCGGCTTGGCGGTTGGAGCGGCTGTCGCCATGTT1320.4564788878514369No Hit
AAGCACGGGGTATGGTACCACTGTGTTGGTTATATGGTGAATCTGAACGTAC1310.45302071445862296No Hit
GAAGTGGGGACTGTCGGACAGAAGCCGGTTTCTCTGGGATCCAAGACGTGTA1310.45302071445862296No Hit
AGCCTAGGGTGCCACCACTGCCCTGCTCCACTATCAGCTGCCCCAGATGCCG1310.45302071445862296No Hit
TAGACAGGGCTCTTCGTTAAGTCGGCCTTCCCAACATGGCGCAGTCTATTAA1300.44956254106580906No Hit
TCTAGAGGGATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAA1300.44956254106580906No Hit
TAAGTAGGGGTTATTAGTTAGCAAAATGAACCTGGGGAAGTGGGAAGAGCTA1290.4461043676729951No Hit
AGTAATGGGTCCTTCAGGATATCAAGAAACCAGACTGTGATGACTGGGAGAG1290.4461043676729951No Hit
GAGTGCGGTCGCGCTGGGGATCCACTGTTAAAGAACTGCATAGGTGATTTCC1290.4461043676729951No Hit
TATCAAAGGGTGCTTCTGTTTTTTCACCATCATTTGGTCTGAAACAGACAGA1280.4426461942801812No Hit
AGCTTTGGGAGTGTCTCCGCGGCGCACCTCGCGGCGAGAATCCGGAGGAGAA1270.43918802088736725No Hit
CTATCTGGGTGGTCTGGCAACTACTTCACGGAGACGGCAGGCACCTCGCTGC1260.4357298474945534No Hit
TGAGGTGGGCAATTGAAAAAATTAACAATGTTTTATTTTTGTGAAATTTTTG1250.4322716741017395No Hit
TGTGTGGGGGGAGCGGCGGAGGCGCCCAGCGGAGGTGAAAGTATTGGCGGAA1240.42881350070892554No Hit
CTTTTGGGTCCAATATCCACCTTCCCAAACCTCATGGAAGCACAAGAATCCT1230.42535532731611164No Hit
GAACTAGGGATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACTTAT1200.4149808071376698No Hit
CAGAAAGGGTGTCACTGGGATCACCTTCCTGTCTGGAGGCCAGAGTGAGGAG1180.4080644603520421No Hit
GCCAAGGAGTCTTTTTAAAAATAATTTCCTTTTACAATTCCCTTTGTCTTTA1170.4046062869592281No Hit
TAATATGGGAGAGCAGTACCTTTCCAGATTCGAAGAAGCAATGAAGCTAAGG1170.4046062869592281No Hit
CGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTC1160.4011481135664142No Hit
ATGTCGGGGGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT1160.4011481135664142No Hit
TTTACAGGGATTCTGGAAGAGGAGGAAGAAATTGATAATACAGTTCCTCCTG1140.39423176678078636No Hit
TAAAGCGGGGGTTAGGAAGGAAGACGTGAGTTTAGGAGACACATGTAGAGCC1140.39423176678078636No Hit
CTAGGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1130.3907735933879725No Hit
GCGCGAGGGGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGT1120.38731541999515856No Hit
TCACTAGGGGACGGCGCAGGGGCTGCGGGGAGCGCCGCGCAGGCCGTGCAGT1120.38731541999515856No Hit
GTAAGATGGGGAGGCTACAGTTGATATTTAAAAGATCCATTTAAAACAAGCT1110.38385724660234466No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT1100.3803990732095307RNA PCR Primer, Index 17 (95% over 22bp)
ACCACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1100.3803990732095307No Hit
TTCCCGAGGGGGTCTCTTCTATTATCGCCTCCTCTTAGTTGGCATTGATGAA1100.3803990732095307No Hit
CTGCGCCGGGTCTGGAAGCTTTCTAGCTTTACCGCTTCTATTGATCCACACT1090.3769408998167168No Hit
CTTAATGGGCGCGGCGGCGATGTCCGCGAGCCAGGCGAGTGCCGCGGCGGCA1070.370024553031089No Hit
TTTCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1060.36656637963827504No Hit
GTGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1050.36310820624546114No Hit
GATGATGGGCTGTCCCACAAATAGTTTTTTGTTTACGATTTATGACAGGTTT1050.36310820624546114No Hit
TACCCAGGGGTACTCGTCATCGGCCATTGCACTGGGGAACTCCCTCACCCCA1050.36310820624546114No Hit
TGGAACGGGAGGTAGGAGGTCCTGGGTGACTTTGGAAGTCCGTAGTGTCTCA1030.35619185945983334No Hit
ATCGCAGGGAGTCCTTGTGACCTAATCATCTCCCAGAAGGCTCCGCCTCCCA1020.3527336860670194No Hit
TGAACAGGGCTTTTCAGTCCTTGCGCACCGGGGAACAAGGTCGTGAAAAAAA1020.3527336860670194No Hit
CTTTGGGGGATCCATTTTGAAGAGACGGGGGAGCGGGGGGCTCGTCTGTTCC1010.3492755126742055No Hit
TTTTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1010.3492755126742055No Hit
CCAATAGGGAAGTGCTGGAATTTTCCAAAACAGCCTGTTCTCTCCTCTCTCC980.3389009924957638No Hit
AGCAATGGGATGGACAAGAAGCCAACTTAGAAAGAAGGGTCTCACGTGGCTG980.3389009924957638No Hit
AGCATAGGGTATACAGCGGAGTCTTCAGATACACTGTGTCCTCGATGTGCAG970.3354428191029498No Hit
TGTTATGGGACGTCCCACAAATGCAGGTGTCCTGAGCACCACACCCAGTGGG970.3354428191029498No Hit
TAACGCGGGGGAGCCGCCGCCATGTCTGCGCATCTGCAATGGATGGTCGTGC970.3354428191029498No Hit
TCGTCAGGGGTTGTCTCAGGATTGGAGGGTGAAAGGGGCATTGTTATAGGGT950.328526472317322No Hit
CATCGCGGGAAGGGCTTCCCAGGTGGTGCTGGACCCTGACGACAGCCATTTG950.328526472317322No Hit
GCTACAGGGATACCAACTGGGCTATTGGATTAAGTGTGGCTGATCTTATTGA940.32506829892450806No Hit
CGAACTGGGGACGCTCTTCCGATCTTTTGCCGGGAGATGCGCCACGGTTTCG920.31815195213888026No Hit
GTAATGGGGATTCTCATAGGAAATAAAGCAGATTTGGAGGCACAGAGAGATG900.3112356053532524No Hit
CTATGCGGGTAGTGTGGCATTGCAGTGATGGCTGTTTAATAAAGGTCCATCG900.3112356053532524No Hit
GCAATTGGGAATGGAGAAACCCTAGAAAAGATCACCAACAGCCGTCCTCCCT880.3043192585676246No Hit
TTGGTTGGGTTCAAAGTTCTGGGGCTTTGGGCGGAAAACAGCCAGCTTTGGC870.30086108517481064No Hit
GTTAACGGGCCGATCTGTTCAAGGGGGATCCGTGGTGTGCCGCTACTGCCGG850.29394473838918284No Hit
CACCGAGGGGCTTTTATGTACTGACTTCATAAAAGCCATCTCAGAGCCATTG850.29394473838918284No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA840.29048656499636893No Hit
TTGTAAGGAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGG830.287028391603555No Hit
CGGTGAGGGACGGTGACCATTTTTTTGTGTGTACAGCGTCATATAGGCTTTG830.287028391603555No Hit
CACCCAAGGGAGAGCCTGTTCTTCGAGACATCGTCCATTTCGTGCCCTTCAG830.287028391603555No Hit
TCATGTGGGATAAATTCAATGGAATTTGAGAAGCAATGGCAGCGGGATAATT820.2835702182107411No Hit
GCGCCAGGGATTCTTGTATGGCTTTGGTAGGCATACTTGGAGAACATATCCC820.2835702182107411No Hit
CGATGGGGATTCGCTTGACTCACGCCTTCGCCGTAGCATCTTTCGCAGCGGA820.2835702182107411No Hit
TTGAATGGGTATCTGAATGATAAAGGCCTAAGTGATAACTCTGGACAAGGGA810.2801120448179272No Hit
GCAAAAGGGAAGTTGATCCTTTTACTTACTTATGTAGAAGAGGCACATGGTA800.2766538714251132No Hit
TTTCGCGGGCTAACAATTATATGGTAAATGAATAAGCTCGTTATTTGAAAAT760.26282117785385756No Hit
TTGTATGGGGGACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCGTTCTGAGC760.26282117785385756No Hit
GTAGGCGGGACCTTTGTGCACAAATAACATTATATATATTATATATCTATTC750.2593630044610437No Hit
TTACATGGGTGTCATGAGGTCACTGGGTCAGAACCCAACAGAAGCTGAATTG740.25590483106822975No Hit
ATGTCAAGGACTGTGGCGAGTTTGATAATGCTGGTGGAATGATGAGTGTGGA740.25590483106822975No Hit
AAATATGGGGATCTGTTTTGGATGTTGCTATAGTGACATGCAGTTATATATT740.25590483106822975No Hit
GGTTTGGGGGGGAAGGGATCTATGGGAAATCTAGGCGAAAGTAATGGGGAAG730.25244665767541585No Hit
TCAGCAAGGGCTTCTGGCTTTTCCCTTGAGGATCCCCAGACCCACTCCAACC720.24898848428260195No Hit
GTATGTGGGCAATATCTGTCACCTAGTAAGCAACCAAGAGACCCTCCCTTCA650.22478127053290453No Hit
ATGCTTGGGTATATTTGCTTTTTTTCTCACCTGCACTTAGAGGAAATTTGAA650.22478127053290453No Hit
TCTTGTGGGATAGAATGTGTGAAACACTGTATTAGTAAACTTCATCACCTTT640.2213230971400906No Hit
CCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACT630.2178649237472767No Hit
GCCGCCGGGACAGGCTCCTGGAGGGCTGCCTGGGGGAGGCAGACATGGGAGT630.2178649237472767No Hit
TCAAGAGGGAAGAATGTGTGTCCAATACGTGATCAAGTAGTTTAATATGGTA620.21440675035446277No Hit
GACTCAGGGACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAG620.21440675035446277No Hit
CCTCAGGGGTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCC610.21094857696164884No Hit
GTGGGTGGGAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACC610.21094857696164884No Hit
CACATAGGGGGGCCGGGGGGTGCGGGCGCCCTCGCCGCCCGGCTACCTACCG600.2074904035688349No Hit
CTGTAAGGGATCTCGGCTCACTGCAAGCTCCGCCTCCCGGGTTCATGCCATT600.2074904035688349No Hit
TTACGCGGGGGAACGCACTCACAGTCGCATCATAATCCTCTCTCAAGGACTT600.2074904035688349No Hit
TAATCCGGGGGGTTGATTACACAATGCCTTGTACATGATATAGAGGCATCAA580.2005740567832071No Hit
GCACATGGGGGGGCTGGTCCCGCAGGCGCTCGGGGTTGGAGCCAGCGACCGT580.2005740567832071No Hit
GGCTCAGGGATATACCTAATGCTAAATGACGAGTTGATGGGTGCAGCACACC560.19365770999757928No Hit
AAGTAAGGGGCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGT550.19019953660476535No Hit
ACAACAGGGCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTT540.18674136321195145No Hit
TGATCAGGGATGGTCTTGTTTGTTGACATGACAAATTAACATTCTTATAGTT540.18674136321195145No Hit
AGCCAAGATACTGATATTTTAAAACAAAATGGAAAAATGTCCAATACAATAT530.18328318981913752No Hit
CTTCCTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA520.17982501642632362No Hit
GTCGTTGGGATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCATTCTCATAA510.1763668430335097No Hit
CATAAGGGCTGGTAAGGTGCTGTTCACTTGAACAGGTGCTGTTGCGCAGAAA500.1729086696406958No Hit
TTATTCAGGGGAAGTATATACTACCTATGAGTCCAATTAACATGAGTATTAT490.1694504962478819No Hit
GCAGAAGTATAAAAGAATAAAGAAACCTGAAGGAAAGCCAGATCAGAAGTTT480.16599232285506796No Hit
CACAGCGGGTCGTATACCTGAGGTGCACCGGAGGTGAAGTCGGTGCCACTTC470.16253414946225403No Hit
TGTACAGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCT440.1521596292838123No Hit
TTTGTCTGGGAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCG400.1383269357125566No Hit
TGCCATGGGGCTTCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAG390.1348687623197427No Hit
AAGATAGGGAAGCGCAAAAGAAGAAAGATGAGGCAGAGGTCCAAGTAAACCG380.13141058892692878No Hit
CTAGTGGGGAGAACCCTGGTGAGGAAGCTCCAGTCCTGCTCTCTACCCAGCC370.12795241553411488No Hit
TGTGCTGGGAGGCTGCTGGGGCCTCAGACGAGACCCAAGGGCAGAGCATTTA370.12795241553411488No Hit
ACTCTTGGGCATTTATTTGTGTCTTCTTCAATTTATTTCATCAATGTTTTAC370.12795241553411488No Hit
TTGATTGGGGGGCCCCCGCGGACCCAGGTGCTCTGGGTCACGGTCCCTGTCC360.12449424214130098No Hit
GCGCGGGGGTCTTCCGATCTACACGTGGGCGAAGGAAGATTATGTCTTCCCC360.12449424214130098No Hit
ATCGTTGGGGCTATGTTTTGGAGAGAGCAATCTTGCTGTGAAACAGTGTGGA360.12449424214130098No Hit
GCCTAAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA350.12103606874848705No Hit
GTTGCCGGGCACCTAATATTGTGGAATCAGCAGCAGAAAACATGGTCAAGCT290.10028702839160356No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCTTATAC290.10028702839160356No Hit
TTAAGCGGGCCGGTCCCCTGCATGGGAATTGCCCTGAACCCTCAGTAGTGGT290.10028702839160356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)