FastQCFastQC Report
Sat 31 Dec 2022
SRR5844020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28797
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGCTGGCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCA2120.7361877973400006No Hit
GGCAATGGGAGGCCGTCCGGGCGGCGCAGTCCCTCGGATCGCTCTCTCCTCA2020.7014619578428308No Hit
GACAAGGGGGCAGGGTCTCCTTGGTATGCCTACACACACTTTACAGGGAACT1960.6806264541445289No Hit
TAAAATGGGGAGTTATTATTTTCCAAGTTTGAACATGTTAAATATCAGATGC1950.677153870194812No Hit
CGCATAGGGCTGTGTGATTTATTTCACTTGGTTATATTTATTTCTGATGGTT1930.670208702295378No Hit
TCTTATGGGCAGGTGTTCATACAGTGGCATTTGGGACACAATCGTTGGAACC1800.6250651109490573No Hit
TAGCGGGAGGCAGGAACACTGGAGCCTGCAATAAGGGAGGAGAGGGGACTGG1750.6077021912004723No Hit
CTGTTGGGGGCTCTATCTCCATTTATGTTACATCTTCTGCCAAATAGCTTTG1740.6042296072507553No Hit
AGATATGGAGGAGAAGGAAAGGAGAGAAGGCAGGAAGAAGGCAAGGGACGAG1730.6007570233010383No Hit
ACATTAGGGCATTTTTCCCCGCTTAGTTTGTTGACTCCGTGTTCATCAGCAT1690.5868666875021703No Hit
TGCTGCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1640.5695037677535855No Hit
AGCCTAGGGTGCCACCACTGCCCTGCTCCACTATCAGCTGCCCCAGATGCCG1580.5486682640552836No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC1540.5347779282564156No Hit
TCAGTGGGGAATGACTGACAGAATATTTTGTTGGGCAGTCCTGATTTAAAAC1520.5278327603569817No Hit
GGGCTGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1510.5243601764072647No Hit
AAGCACGGGGTATGGTACCACTGTGTTGGTTATATGGTGAATCTGAACGTAC1480.5139424245581137No Hit
TAGACAGGGCTCTTCGTTAAGTCGGCCTTCCCAACATGGCGCAGTCTATTAA1470.5104698406083967No Hit
GCGGGTGGGGGAAAAAAGGTCCCGGAGAGCTGAGCAGTCAAGATGTGTGACT1470.5104698406083967No Hit
TATCACGGATAGTCACAATGGCAAAGGGAGAACTGGGGGGCTGGCAGGTAAT1450.5035246727089627No Hit
TGATATGGGATTGCACTGTTTCTCCACTTTTTATTTTCTCTTAGGCTTTTGT1450.5035246727089627No Hit
TAAAATGGGCTTTGGTATCGTGGAAGGACTCATGACCACAGTCCATGCCATC1430.4965795048095288No Hit
TTAAGTGGGAAGAAGGCACTTGTTTGTAAGCCAGAGAAGAAACTTTAATTGC1430.4965795048095288No Hit
TTCTGGGGGGGGAGCGGCCAGTGGACTCTCGCCGCCACCCGGTCCAGGTGCC1400.4861617529603778No Hit
GTGTTAGGGCCGGCCTCTCCCTGTACACGTGCGGGAGGAAGGTGTGGTGCTG1390.4826891690106608No Hit
TGCGCAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1380.4792165850609438No Hit
CTAGGTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1380.4792165850609438No Hit
GTATCGGGGGATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGG1380.4792165850609438No Hit
TTCCAAGGGGCTCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATG1380.4792165850609438No Hit
CTTTTGGGTCCAATATCCACCTTCCCAAACCTCATGGAAGCACAAGAATCCT1360.4722714171615099No Hit
GGATACGGGGAGTCTCGCTCTGTCACCCGGGCTGGAGTGTAACGTGGCATGA1350.46879883321179294No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAG1350.46879883321179294No Hit
TTTACAGGGATTCTGGAAGAGGAGGAAGAAATTGATAATACAGTTCCTCCTG1340.46532624926207594No Hit
AGCATAGGGTATACAGCGGAGTCTTCAGATACACTGTGTCCTCGATGTGCAG1340.46532624926207594No Hit
GCGCGAGGGGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGT1340.46532624926207594No Hit
TGCTTAGGGAGACTCTCAAAATCGGAAGAAGCTTTCAGAGCTGTTAAGGTAC1320.45838108136264194No Hit
TAATATGGGAGAGCAGTACCTTTCCAGATTCGAAGAAGCAATGAAGCTAAGG1310.45490849741292494No Hit
CTGCGCCGGGTCTGGAAGCTTTCTAGCTTTACCGCTTCTATTGATCCACACT1310.45490849741292494No Hit
TTCCCGAGGGGGTCTCTTCTATTATCGCCTCCTCTTAGTTGGCATTGATGAA1310.45490849741292494No Hit
GCATACGGGAGAGACAATTATGACAACTGAAATGAGACATGCACATAATATA1300.451435913463208No Hit
AGCTTTGGGAGTGTCTCCGCGGCGCACCTCGCGGCGAGAATCCGGAGGAGAA1300.451435913463208No Hit
CATTGAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1280.444490745563774No Hit
ATATATGGACAGGTGTGAGCCACCATGCCCAGCCAGATCTTTCTTGTTTTTG1280.444490745563774No Hit
ATCATGGGCCAATGTGTTTGCCATGTTTGACCAGTCACAGATTCAGGAGTTC1270.441018161614057No Hit
TGAGGTGGGCAATTGAAAAAATTAACAATGTTTTATTTTTGTGAAATTTTTG1270.441018161614057No Hit
GAGTGCGGTCGCGCTGGGGATCCACTGTTAAAGAACTGCATAGGTGATTTCC1270.441018161614057No Hit
CTATCTGGGTGGTCTGGCAACTACTTCACGGAGACGGCAGGCACCTCGCTGC1260.43754557766434No Hit
GAAAGTGGGAAGCTGAATGGTATCTATCACTTATTTATCCTTGACAGGTTAT1260.43754557766434No Hit
TGAACAGGGCTTTTCAGTCCTTGCGCACCGGGGAACAAGGTCGTGAAAAAAA1260.43754557766434No Hit
CCAACAGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA1250.43407299371462305No Hit
TAAAGCGGGGGTTAGGAAGGAAGACGTGAGTTTAGGAGACACATGTAGAGCC1250.43407299371462305No Hit
TACTAAGGGACTTCCACTCCATAACGCTCCTCATACTAGGCCTACTAACCAA1240.43060040976490604No Hit
TAAGTAGGGGTTATTAGTTAGCAAAATGAACCTGGGGAAGTGGGAAGAGCTA1240.43060040976490604No Hit
AGCAACGGGGCTGACTTACAGACACAGGCTGTTTACAAAATGCTAGCTGGAA1240.43060040976490604No Hit
TCTAGAGGGATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAA1240.43060040976490604No Hit
CAGAAAGGGTGTCACTGGGATCACCTTCCTGTCTGGAGGCCAGAGTGAGGAG1230.4271278258151891No Hit
TAAGTGGGGTCCGATCTTGCTGTGGGAACCTGGCGCCGTTGGCATCGTAGAG1230.4271278258151891No Hit
GAACTAGGGATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACTTAT1190.41323749001632115No Hit
CATTAGGGGAGGAGGAGGAGGGACTCGGCGGGAGGATGGGCTTGCTCTCAAT1180.40976490606660415No Hit
CGCCCTCATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTC1170.4062923221168872No Hit
CCAATAGGGAAGTGCTGGAATTTTCCAAAACAGCCTGTTCTCTCCTCTCTCC1150.3993471542174532No Hit
GAAGTGGGGACTGTCGGACAGAAGCCGGTTTCTCTGGGATCCAAGACGTGTA1150.3993471542174532No Hit
TCGTCAGGGGTTGTCTCAGGATTGGAGGGTGAAAGGGGCATTGTTATAGGGT1150.3993471542174532No Hit
TGGAACGGGAGGTAGGAGGTCCTGGGTGACTTTGGAAGTCCGTAGTGTCTCA1140.3958745702677362No Hit
AGTAATGGGTCCTTCAGGATATCAAGAAACCAGACTGTGATGACTGGGAGAG1110.38545681841858526No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT1100.3819842344688683RNA PCR Primer, Index 17 (95% over 22bp)
ATGTCGGGGGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT1090.3785116505191513No Hit
TATCAAAGGGTGCTTCTGTTTTTTCACCATCATTTGGTCTGAAACAGACAGA1070.3715664826197173No Hit
GCCAAGGAGTCTTTTTAAAAATAATTTCCTTTTACAATTCCCTTTGTCTTTA1060.3680938986700003No Hit
ACCACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1060.3680938986700003No Hit
GATGATGGGCTGTCCCACAAATAGTTTTTTGTTTACGATTTATGACAGGTTT1030.3576761468208494No Hit
AACTTTGGGGCCCTGTGACCGGCTTGGCGGTTGGAGCGGCTGTCGCCATGTT1020.3542035628711324No Hit
CTATGCGGGTAGTGTGGCATTGCAGTGATGGCTGTTTAATAAAGGTCCATCG1020.3542035628711324No Hit
CTTAATGGGCGCGGCGGCGATGTCCGCGAGCCAGGCGAGTGCCGCGGCGGCA1010.3507309789214154No Hit
CTTTGGGGGATCCATTTTGAAGAGACGGGGGAGCGGGGGGCTCGTCTGTTCC1010.3507309789214154No Hit
GCTACAGGGATACCAACTGGGCTATTGGATTAAGTGTGGCTGATCTTATTGA1010.3507309789214154No Hit
TCACTAGGGGACGGCGCAGGGGCTGCGGGGAGCGCCGCGCAGGCCGTGCAGT990.34378581102198147No Hit
ATCGCAGGGAGTCCTTGTGACCTAATCATCTCCCAGAAGGCTCCGCCTCCCA980.34031322707226447No Hit
TACCCAGGGGTACTCGTCATCGGCCATTGCACTGGGGAACTCCCTCACCCCA980.34031322707226447No Hit
GTAATGGGGATTCTCATAGGAAATAAAGCAGATTTGGAGGCACAGAGAGATG970.3368406431225475No Hit
TTGTAAGGAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGG970.3368406431225475No Hit
CATCGCGGGAAGGGCTTCCCAGGTGGTGCTGGACCCTGACGACAGCCATTTG970.3368406431225475No Hit
TAACGCGGGGGAGCCGCCGCCATGTCTGCGCATCTGCAATGGATGGTCGTGC960.3333680591728305No Hit
CGATGGGGATTCGCTTGACTCACGCCTTCGCCGTAGCATCTTTCGCAGCGGA950.3298954752231135No Hit
TGTGTGGGGGGAGCGGCGGAGGCGCCCAGCGGAGGTGAAAGTATTGGCGGAA940.3264228912733965No Hit
CTAGAGGGGCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTT940.3264228912733965No Hit
AAGTAAGGGGCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGT940.3264228912733965No Hit
TTGAATGGGTATCTGAATGATAAAGGCCTAAGTGATAACTCTGGACAAGGGA930.3229503073236795No Hit
CGGTGAGGGACGGTGACCATTTTTTTGTGTGTACAGCGTCATATAGGCTTTG920.3194777233739626No Hit
GTAAGATGGGGAGGCTACAGTTGATATTTAAAAGATCCATTTAAAACAAGCT910.3160051394242456No Hit
TTTTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.3160051394242456No Hit
GTAGGCGGGACCTTTGTGCACAAATAACATTATATATATTATATATCTATTC910.3160051394242456No Hit
TCAGCAAGGGCTTCTGGCTTTTCCCTTGAGGATCCCCAGACCCACTCCAACC900.31253255547452863No Hit
TTGGTTGGGTTCAAAGTTCTGGGGCTTTGGGCGGAAAACAGCCAGCTTTGGC890.3090599715248116No Hit
TTTCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC890.3090599715248116No Hit
TTACATGGGTGTCATGAGGTCACTGGGTCAGAACCCAACAGAAGCTGAATTG870.3021148036253776No Hit
AGCAATGGGATGGACAAGAAGCCAACTTAGAAAGAAGGGTCTCACGTGGCTG870.3021148036253776No Hit
TGTTATGGGACGTCCCACAAATGCAGGTGTCCTGAGCACCACACCCAGTGGG870.3021148036253776No Hit
ATGCTTGGGTATATTTGCTTTTTTTCTCACCTGCACTTAGAGGAAATTTGAA860.2986422196756606No Hit
GTGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA850.2951696357259437No Hit
TTGTATGGGGGACCTTTCCGAGTTGGCTGCAGATTTGTGGTGCGTTCTGAGC850.2951696357259437No Hit
GCAATTGGGAATGGAGAAACCCTAGAAAAGATCACCAACAGCCGTCCTCCCT840.2916970517762267No Hit
CGAACTGGGGACGCTCTTCCGATCTTTTGCCGGGAGATGCGCCACGGTTTCG840.2916970517762267No Hit
GTTAACGGGCCGATCTGTTCAAGGGGGATCCGTGGTGTGCCGCTACTGCCGG830.28822446782650973No Hit
GCGCCAGGGATTCTTGTATGGCTTTGGTAGGCATACTTGGAGAACATATCCC830.28822446782650973No Hit
GCAAAAGGGAAGTTGATCCTTTTACTTACTTATGTAGAAGAGGCACATGGTA830.28822446782650973No Hit
TTTCGCGGGCTAACAATTATATGGTAAATGAATAAGCTCGTTATTTGAAAAT820.28475188387679273No Hit
CACATAGGGGGGCCGGGGGGTGCGGGCGCCCTCGCCGCCCGGCTACCTACCG790.2743341320276418No Hit
CACCGAGGGGCTTTTATGTACTGACTTCATAAAAGCCATCTCAGAGCCATTG780.2708615480779248No Hit
GTGGGTGGGAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACC770.2673889641282078No Hit
ATGTCAAGGACTGTGGCGAGTTTGATAATGCTGGTGGAATGATGAGTGTGGA760.26391638017849084No Hit
CTCCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCA750.26044379622877384No Hit
TCATGTGGGATAAATTCAATGGAATTTGAGAAGCAATGGCAGCGGGATAATT740.25697121227905684No Hit
CACCCAAGGGAGAGCCTGTTCTTCGAGACATCGTCCATTTCGTGCCCTTCAG730.2534986283293399No Hit
GCCGCCGGGACAGGCTCCTGGAGGGCTGCCTGGGGGAGGCAGACATGGGAGT730.2534986283293399No Hit
GGTTTGGGGGGGAAGGGATCTATGGGAAATCTAGGCGAAAGTAATGGGGAAG720.2500260443796229No Hit
CCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACATGACT720.2500260443796229No Hit
GGCTCAGGGATATACCTAATGCTAAATGACGAGTTGATGGGTGCAGCACACC680.23613570858075494No Hit
AAATATGGGGATCTGTTTTGGATGTTGCTATAGTGACATGCAGTTATATATT670.23266312463103797No Hit
GTATGTGGGCAATATCTGTCACCTAGTAAGCAACCAAGAGACCCTCCCTTCA640.222245372781887No Hit
CCTCAGGGGTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCC640.222245372781887No Hit
GTCGTTGGGATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCATTCTCATAA640.222245372781887No Hit
CTAGTGGGGAGAACCCTGGTGAGGAAGCTCCAGTCCTGCTCTCTACCCAGCC630.21877278883217No Hit
AGCCAAGATACTGATATTTTAAAACAAAATGGAAAAATGTCCAATACAATAT630.21877278883217No Hit
TCAAGAGGGAAGAATGTGTGTCCAATACGTGATCAAGTAGTTTAATATGGTA610.21182762093273608No Hit
TTACGCGGGGGAACGCACTCACAGTCGCATCATAATCCTCTCTCAAGGACTT600.20835503698301908No Hit
GACTCAGGGACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAG590.20488245303330208No Hit
TCTTGTGGGATAGAATGTGTGAAACACTGTATTAGTAAACTTCATCACCTTT570.1979372851338681No Hit
TAATCCGGGGGGTTGATTACACAATGCCTTGTACATGATATAGAGGCATCAA560.19446470118415113No Hit
CTGTAAGGGATCTCGGCTCACTGCAAGCTCCGCCTCCCGGGTTCATGCCATT550.19099211723443416No Hit
TTTGTCTGGGAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCG510.1771017814355662No Hit
ACTCTTGGGCATTTATTTGTGTCTTCTTCAATTTATTTCATCAATGTTTTAC510.1771017814355662No Hit
AGTCTAGGGCCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG500.1736291974858492No Hit
TGTACAGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCT480.16668402958641526No Hit
GCACATGGGGGGGCTGGTCCCGCAGGCGCTCGGGGTTGGAGCCAGCGACCGT480.16668402958641526No Hit
ACAACAGGGCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTT460.1597388616869813No Hit
TGATCAGGGATGGTCTTGTTTGTTGACATGACAAATTAACATTCTTATAGTT460.1597388616869813No Hit
CACAGCGGGTCGTATACCTGAGGTGCACCGGAGGTGAAGTCGGTGCCACTTC460.1597388616869813No Hit
GCCTAAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA440.1527936937875473No Hit
AAGATAGGGAAGCGCAAAAGAAGAAAGATGAGGCAGAGGTCCAAGTAAACCG430.1493211098378303No Hit
CATAAGGGCTGGTAAGGTGCTGTTCACTTGAACAGGTGCTGTTGCGCAGAAA410.14237594193839637No Hit
GCGCGGGGGTCTTCCGATCTACACGTGGGCGAAGGAAGATTATGTCTTCCCC400.13890335798867937No Hit
CTTCCTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA400.13890335798867937No Hit
TTATTCAGGGGAAGTATATACTACCTATGAGTCCAATTAACATGAGTATTAT400.13890335798867937No Hit
TTGATTGGGGGGCCCCCGCGGACCCAGGTGCTCTGGGTCACGGTCCCTGTCC380.13195819008924542No Hit
TGTGCTGGGAGGCTGCTGGGGCCTCAGACGAGACCCAAGGGCAGAGCATTTA370.12848560613952842No Hit
GCAGAAGTATAAAAGAATAAAGAAACCTGAAGGAAAGCCAGATCAGAAGTTT370.12848560613952842No Hit
ATCGTTGGGGCTATGTTTTGGAGAGAGCAATCTTGCTGTGAAACAGTGTGGA330.11459527034066049No Hit
TGCCATGGGGCTTCCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAG310.10765010244122651No Hit
CCACACGGGCGATCTGAATAAGGGTCGGGGGTGGCGACCAAGTGACCCGCTC300.10417751849150954No Hit
TTAAGCGGGCCGGTCCCCTGCATGGGAATTGCCCTGAACCCTCAGTAGTGGT300.10417751849150954No Hit
GTCTATGGGCTAGCGCAGGGAGTCTTCCCAGCAATTATTACAATTCTTGAAT300.10417751849150954No Hit
TAGTAGGGGGCTATATTGCAATTGGACTGGAGGCTATAAGTGGTCTACGGCT290.10070493454179255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)