FastQCFastQC Report
Sat 31 Dec 2022
SRR5844019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255441
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC4630.1812551626402966No Hit
AAAAATGGGGCAGAAAATGAGATGCTAAACTGCTGCCCTAGGGGATTGGTTA3370.13192870369282927No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT3190.12488206670033394RNA PCR Primer, Index 17 (95% over 22bp)
AACTTGGGGGATAGGCCGAGTTCCGGGCGCGAGGCGGCCACCGTGGAGAGCA2970.11626951037617297No Hit
TAGCCAGGGAAGTTAAATTTAAAAGGATAATGGCAGAATTCTTTCTTGGGTT2920.11431211121159095No Hit
TTAGCAGGGGAATTCAAAGGATAAAAAAATTGATAATGAGATTTTCAAGGTT2880.1127461918799253No Hit
TAAGGTGGGGATTTAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTC2840.11118027254825968No Hit
GATACTGGGAGATGGAAGCTATTTAGTTCCTTGTTTTTTCTAATTACACACT2830.11078879271534327No Hit
GAGCATGGGAACCAGAAGGGTTGTTATGATCTGGTAACTAGTTTCATGGAGA2770.10843991371784482No Hit
ACAAATGGGCACATTTGGGCAGTGCCACCCACTCCTTGGGCCCCTAGGATGA2730.10687399438617919No Hit
CGTATAGGGGCCCCTAATCCAGTATGAGTGGTGTCCTTATAAAAAGGGGAAA2720.1064825145532628No Hit
GTCAACGGGATGTGTAAACATTATGATATTATTTTATAAACTGACTAAGCCA2710.1060910347203464No Hit
ACGCGGGGGGGCGGCGGCCGTTGCGATTGATTGCGCTGGTTGCCTGCGGCGT2690.10530807505451356No Hit
TAACGCGGGCTCCCAGCGCAGCGCCGCCGCCCGGCCCCTCCAGCTTCCCGGA2680.10491659522159716No Hit
TTGCAAGGGGAGAGTGAAACCTGTATTTGTCAAATGAATGAGTGAAGTAATA2680.10491659522159716No Hit
ACAGCGGGGTTGAAAACTGGGCATTTGAAATAACATACTATGGCAGCTCTGA2660.10413363555576434No Hit
AGACTTGGGACTCCATATCTACTGAATCAGAATCTGCATTTTAACAAGAAAT2630.10295919605701513No Hit
TCTTGGGAGCGCGGCCTGGTGGGCGAGATCATCAAGCGCTTCGAGCAGAAGG2600.1017847565582659No Hit
AGCAATAGGGAACTAAGAAATTAATTTGAGTGAAATTAATGTTAGAATAAAA2590.10139327672534948No Hit
GAACTTGGGACCTAGATTTAATTCTGACTGTCTTACAAAAGGTAAAACATAT2570.10061031705951669No Hit
AGAGTTGGGGTGAACGGGATCCAGGTGGGGAAAGAGGTCAAGACAGGGAGCG2560.10021883722660027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)