FastQCFastQC Report
Sat 31 Dec 2022
SRR5844018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255928
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC4490.1754399674908568No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT3190.12464443124628802RNA PCR Primer, Index 17 (95% over 22bp)
ACAATAGGGAATTATGTGGATTCTGTATAATCTCCTCACAAGGATGCTTTTG3100.12112781719858709No Hit
AACTTGGGGGATAGGCCGAGTTCCGGGCGCGAGGCGGCCACCGTGGAGAGCA3060.11956487762183113No Hit
TAGCCAGGGAAGTTAAATTTAAAAGGATAATGGCAGAATTCTTTCTTGGGTT3050.11917414272764215No Hit
AAAAATGGGGCAGAAAATGAGATGCTAAACTGCTGCCCTAGGGGATTGGTTA3040.11878340783345316No Hit
TAACGCGGGCTCCCAGCGCAGCGCCGCCGCCCGGCCCCTCCAGCTTCCCGGA2980.11643899846831922No Hit
TAAGGTGGGGATTTAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTC2930.11448532399737427No Hit
TTGCAAGGGGAGAGTGAAACCTGTATTTGTCAAATGAATGAGTGAAGTAATA2870.11214091463224032No Hit
TTAGCAGGGGAATTCAAAGGATAAAAAAATTGATAATGAGATTTTCAAGGTT2830.11057797505548436No Hit
AACCTAGGGATCTGGGAGGACTTATTTGAGAAAGTGACTTCTGGTCTTAGAT2820.11018724016129537No Hit
AGACTTGGGACTCCATATCTACTGAATCAGAATCTGCATTTTAACAAGAAAT2750.10745209590197244No Hit
CAAGTAGGGATGAGTTTTCTGATTAGAAATGTAAAGCCATAAGATTTTAAAT2730.10667062611359444No Hit
TTTGCAGGGTGGCCAGACCGACTAAGGAAAAATGTAAGTGAAAACACAAATT2710.10588915632521648No Hit
GAACTTGGGACCTAGATTTAATTCTGACTGTCTTACAAAAGGTAAAACATAT2670.1043262167484605No Hit
GATACTGGGAGATGGAAGCTATTTAGTTCCTTGTTTTTTCTAATTACACACT2610.10198180738332657No Hit
AGTTTCGGGACAGTAGGTCCATGTTACCTTAAAGCAATCATTGAAGTGAAAA2590.10120033759494858No Hit
CGTATAGGGGCCCCTAATCCAGTATGAGTGGTGTCCTTATAAAAAGGGGAAA2580.10080960270075959No Hit
TGCAATGGGGGAGGGATGGCTACTGGAAAAGAACTGATGAAGTGCAGAGTCT2580.10080960270075959No Hit
ATGCCTGGGCTCGGCTATTTCACTGACTGCGGCATGTCCTCGTGTCTTCTCT2570.10041886780657061No Hit
TAAGTGTGGGGCAGAGAACCAATATAGCAGTGCCCAGGGGTAGAGACCATGT2570.10041886780657061No Hit
GACTGGGTGGTTATGGATCAGGAGACAGTGAAGATGAGAGGAGTGACAGAGG2560.1000281329123816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)