FastQCFastQC Report
Sat 31 Dec 2022
SRR5844017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256562
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC4370.1703291991799253No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT3180.12394664837349256RNA PCR Primer, Index 17 (95% over 22bp)
AAAAATGGGGCAGAAAATGAGATGCTAAACTGCTGCCCTAGGGGATTGGTTA3140.1223875710354612No Hit
TTAGCAGGGGAATTCAAAGGATAAAAAAATTGATAATGAGATTTTCAAGGTT3060.11926941635939851No Hit
TAACGCGGGCTCCCAGCGCAGCGCCGCCGCCCGGCCCCTCCAGCTTCCCGGA2950.11498195367981229No Hit
TAGCCAGGGAAGTTAAATTTAAAAGGATAATGGCAGAATTCTTTCTTGGGTT2910.11342287634178094No Hit
AGCAATAGGGAACTAAGAAATTAATTTGAGTGAAATTAATGTTAGAATAAAA2810.10952518299670254No Hit
TTGCAAGGGGAGAGTGAAACCTGTATTTGTCAAATGAATGAGTGAAGTAATA2800.10913541366219472No Hit
CGTATAGGGGCCCCTAATCCAGTATGAGTGGTGTCCTTATAAAAAGGGGAAA2770.10796610565867121No Hit
GTGCCTGGGAGTGAACGTACTCTTTTATGGTAGTGTTAAAGTCATATAATAA2750.10718656698965554No Hit
ACAGCGGGGTTGAAAACTGGGCATTTGAAATAACATACTATGGCAGCTCTGA2740.10679679765514768No Hit
ACGCGGGGGGGCGGCGGCCGTTGCGATTGATTGCGCTGGTTGCCTGCGGCGT2720.106017258986132No Hit
AACTTGGGGGATAGGCCGAGTTCCGGGCGCGAGGCGGCCACCGTGGAGAGCA2710.10562748965162418No Hit
TTTAGTGGGGGTGAAAATTTTTATGCAAAAAATAAGAGATTGTATAGTAAAA2690.10484795098260849No Hit
TCTGCAGGGGACGCTCAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCA2680.10445818164810064No Hit
TAGGCTGGTCCTGTTGCTGTCGCCGATTAAGTTTTAAACTTTTATTTATTAT2650.10328887364457713No Hit
GTGTCTGGGGAGTCACCTGGATCTATTGTGCATTTTGCATGAATGCAATATA2630.10250933497556146No Hit
ACAATAGGGAATTATGTGGATTCTGTATAATCTCCTCACAAGGATGCTTTTG2620.10211956564105364No Hit
TCGTATGGGTAAGCACCTAGTAAATGTTAGCTGTTTCAATTTTAACCTATCT2600.10134002697203795No Hit
TTTGCAGGGTGGCCAGACCGACTAAGGAAAAATGTAAGTGAAAACACAAATT2600.10134002697203795No Hit
GAACTTGGGACCTAGATTTAATTCTGACTGTCTTACAAAAGGTAAAACATAT2600.10134002697203795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)