FastQCFastQC Report
Sat 31 Dec 2022
SRR5844007.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844007.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17462
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT1550.8876417363417707RNA PCR Primer, Index 17 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC1410.8074676440270301No Hit
TAAAAGGGGGACGGCCTCGCCTCGGTATCGCAGCGGGTCCTCTCTATCTAGC1250.7158401099530409No Hit
AAACATGGGGAAATTTGACAGATCACTCAAAATTTGCATGTGAAGAATATAA1160.6642996220364219No Hit
GGTAATGGGGGGCCCGGCGGCGATGGCGCGGAGCGTGCGCGTGCTGGTGGAC1130.647119459397549No Hit
ATTGATGGGGAATTGTTCCAGACATACCAACTGAACTTTCATTCATTATCAA1090.6242125758790517No Hit
CGCTATGGGGCGTACCTAGGATGCCGCGTGGAAGCCGAAGCCGCACCTCCCG1080.6184858549994273No Hit
AATTCGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1070.612759134119803No Hit
ATTATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1050.6013056923605544No Hit
TAAACCGGGCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGC1040.59557897148093No Hit
AAGTTAGGGTCTTCACACACACAGACACACACTCACACATACACACCCAAAG1030.5898522506013056No Hit
CTTATTGGGGGAGTTTGACGCCGAGTTCCAAAGGCACTACCTGCAGAAGATG1000.5726720879624326No Hit
GATTAAGGGGCTTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGGCG980.5612186462031841No Hit
TACTTAGGGAGACTCCTTCTGTCCAGGAGCGCAGCAGTAGCTGCAGCAGTCA980.5612186462031841No Hit
AAACTAGGGAATCTTTAATGTGTATGCACCATTACAGCATTAAAACACATGA960.5497652044439354No Hit
CACCATGGGGGATTAGAGGCAATAGATCTGACTTGTGGCAAACTTAGTGTTT950.5440384835643111No Hit
TCATTCGGGGCTGAAGGATTGGTTTGGAGCTCTCCACGAGGATGCGAACAGA950.5440384835643111No Hit
TTTTACGGGCAGTACTAAAACCATACGAATTATTGGTTTATTCCAGAAAATA950.5440384835643111No Hit
TGCCCCCAATCCTGCTCCTCCTCTCATGTTTCCTCTTTGACTAAATGGCATT910.5211316000458137No Hit
CCTTTGGGGGCATGCGCGGGGGCCATATTATCAGCGGTTATTCGGTGAGCGG910.5211316000458137No Hit
AGCAAAGGGGCCGCCTCGCGGACCCCGGAAGCGCGCGTGGCCGCCATGGCGC910.5211316000458137No Hit
TATTTCGGGGGATGAGTGAGTTATATAAACTTACTTCCATTCTGTTTCGGAT870.4982247165273164No Hit
TCACGAGGGCTGCAGGGTGACCACTGTGACAGGCTGGGAAGCATAGCCTTTT850.48677127476806786No Hit
GCCTCAGGGAGGGAGATGCGTGGGCTGACATCTGCAGGCCGAAAGAGCCGTG850.48677127476806786No Hit
ATGCTAGGGGAGACTTACAATAACAAGTTGAGTGGCAAGCCTGGTATGCTGT840.48104455388844347No Hit
ACATGTGGGACTGCGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTA840.48104455388844347No Hit
CTACGTGGGCGATCTGGCGTAGGGAATGAAGGCTAGCTAGAGCATTAGCTGG830.47531783300881914No Hit
AAGCCGGGGGCAGGTGTGCTGCTGAGGCGTGAGAATGGCGTCCCGCGGCCGG830.47531783300881914No Hit
TTCACCGGGACTGTAACCTCCACCTCCTGGGTTCACGCAATTCTCCTGCCTC820.46959111212919485No Hit
GGTGCAGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG820.46959111212919485No Hit
AGTAACGGGATCTGTGACAGTCTCTATTCTGTCGTTTTGCTCCTTGGTATTT820.46959111212919485No Hit
TTGCATGGGAGATATATGTGTTCATGTATTTTCTGAAATTGCTTTCATAGAA800.4581376703699461No Hit
GATACGGGGCAGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCGC790.45241094949032185No Hit
GCTCCCGGGTCAGGAAGGTATCACACACTTTCTGACTGCAACTTCTGTCCCT790.45241094949032185No Hit
TTGACAGGGACCTGTCCCCCTGCCTGTCTCCAGTGGGAAGGTAGCCTGGCCA790.45241094949032185No Hit
CTAGTAGGGACATACACAGTCTCAGCAAGGTTATAAAGAACCCTGTCAGGTC770.44095750773107323No Hit
AGCTTCGGGCAGCCGCAGCTACCCAGAAATCCTGACACTCAAGACATACCTG760.43523078685144884No Hit
TGAAACGGGTAATTTAGGAACAAAGCTTTGTCCTACTGACTAGGAAGTAAAC750.42950406597182456No Hit
CAGACTTGGGACAGCCGCGCGCTGCAGGTGGTGCGCGCCCGCAAGCAGATCG750.42950406597182456No Hit
GTTTATGGGAGCAGTTAAACCACTAATAAGGTTTATTGTAAAATGCATGGCA750.42950406597182456No Hit
TGTGCTGGGCTCTGCAGAGTGCCCCATGCAGCTTGAGGACTTCCCGATGGAT740.4237773450922002No Hit
ATAGAAGGGCGATCTGAAACGGGCCACTGTTCCCCGTGCAGACAAAATACAT740.4237773450922002No Hit
TGCTGAGGGACCTCTTGCTACACCCATTCTTTTCATGTGCAGCCGACTCAAA730.41805062421257594No Hit
CTATAAGGGAAGGGTGACGACTTACTCCCGGCAGGGACTGAGGATTACATTC720.41232390333295155No Hit
GTTAGAGGGCTGGGCAAGTCCCTCTGCTCAAGATCACTTTATGGGGCAGCTG710.4065971824533272No Hit
CTACAAGGGGCTTGAGTTACACCTTGTGACCCTTCTCCCATAACATGGTGTG700.40087046157370293No Hit
GTGCCGGGGCGATCTTATATTGGGGGACTTACGTTTATTGCAAAATGAGTTT700.40087046157370293No Hit
TATCCAAGGGATGACCTTTAAAATATGTTGCTTGATTTTCTTTCCCCTGAAA690.3951437406940786No Hit
GATATGGGGAAAATGAAACCAAAAGCCCAGTCCCATTTACAGTAGGAAATAA690.3951437406940786No Hit
TATTAAGGGATTTTAAACTGTTTTTTTCCTATTCTGCTTTTTGCTGCTCTCA680.38941701981445426No Hit
TGGTACGGGGAAGATAGAAGAAAAAAGAATGTCAGAAAAACAATAAGAGCGT680.38941701981445426No Hit
AAGGCAGGGCTAGCCACCAGCCCTGTCCTGCCTTGAGCACCTCCACCTGGGA680.38941701981445426No Hit
ACTTATGGGATAACAATGGGTTCTAAAATCATCAGCATTTTTATGTATTGAG660.3779635780552056No Hit
AGAATGGGGGACGCTCTTCCGATCTGGCGTGGGGGTATATTTTAGCAAAAGG650.37223685717558125No Hit
GCGGTTGGGAGGAGCAAAAAAGCATTTGACAAAATCCAACACCCTTTCATGG630.36078341541633263No Hit
GCTGTGGGTTACAGGCATGAGCCACCACAGCTGGCCCCCTTCTGTTTTATGT630.36078341541633263No Hit
TAATTAGGGAGGGTCCTGAGATTCATCGCCCAGAATCAGAACCGAGACCCCC620.3550566945367083No Hit
TAACGAGGGGATGTGGTTATAGCTGAAAAGCTGGCCTTGGAACAGCAAGTAG620.3550566945367083No Hit
TACGGTGGGATGGCAGTAAGCCTCATGTATACTAAGGAGAGTCTTCCAGGTG620.3550566945367083No Hit
AGTTACGGAAGTTCATAAGATGAGAAAAATCTACGAGCTTCTTATTTACAAC620.3550566945367083No Hit
AGGCGTGGGCACCGCGGTGGTGCTTGTCTCTGCAGACCGCTCGGGCATGGAG610.34932997365708396No Hit
TACTCAGGGGATATTAACAGTTGTGCTTATGTAACATCTGTGTTGTGTGTGT600.3436032527774596No Hit
CAGCATGGGTGGACAGAGGCATTTGACTAAGCCAACCTCCTCTCACAGCCTC600.3436032527774596No Hit
CGCGGTGGGCATATCTTTTACTGTACTCTGTATTTTTATTATGCAAGGAAGA600.3436032527774596No Hit
CTTTTGGAGGGCAGTAGTGCTAATGCATTTTGCACTAGAACGCTTCGGGAAA590.3378765318978353No Hit
GCTTTAGGGGTGGTATGGCAGAATTGAAGATGATGCCTAGATTTGAAGGGGA590.3378765318978353No Hit
ATTGGCGGGTAGCCCTCTCCTTTTAACAAACTCCTTTCTCCATCTGTTCAAA590.3378765318978353No Hit
TGCCAAGGGATATGTATTAACATTTCTCTGATCAAAATTGTGGCTGTTTTCC590.3378765318978353No Hit
TACCTAGGGTGGAAGGGTTGAGGATGGACCGGAGAATTGGAACTTCAGAATA580.33214981101821095No Hit
TAATGCGGGGGAAATGGCCAGGGCCGAGCAGTTAGCACAGCAAGCTCACAGC570.32642309013858667No Hit
CAGACTGGGACCCTGCTGAAGCTCTTTGTCAAATAGGTAGTAGTTAGAACTG570.32642309013858667No Hit
ATTCATGGGAGTTTGTACAGGCAAGTCCTGGGCTGGGTAAAAAGTTATATTA560.3206963692589623No Hit
AAATCTGGGGGAAAAACGAATGCTCTTACACTGAATGTACAGGCAGTGGGCT560.3206963692589623No Hit
TTAATGGGGACTCATTTTATTCATTAGTCTAAGATAAGAATTGTAACACTTC560.3206963692589623No Hit
TAAAATGGGCATGTTTCTGAGAGCTTTAACTACTTCCTTATTTTTATGCAAT550.314969648379338No Hit
CACGAGGGTCCGATCTTAAGCAGGGTTATTTAAAAATCAGTTTTAAAAAATG550.314969648379338No Hit
CGCAGCGGGAGAGTCTCAATCAAACCATAGGTGGGACAGATACCATTATCTC540.30924292749971366No Hit
GAAAGGGCATGGCACAGAGAAATTATATTCCTTACTTCATGTCAGTTTATGT530.3035162066200893No Hit
ATGCTAGGGTGGTGATACAGTCAGTATATAGTAACCTCTCAGGTTTTTGAGA530.3035162066200893No Hit
ATCATAGGGATTCCGTTATGGGGAAAAATTTCATTAATTTTCTCAAATTTAC530.3035162066200893No Hit
TTAGTAGGGATCGACTAAAACTGGCAGAAATTGTGAAGCAGGTGATAGAAGA520.297789485740465No Hit
ACGTTTGGGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCAT520.297789485740465No Hit
TTGACAAGGATGGCAATGGTTATATCAGTGCAGCAGAACTACGTCACGTCAT520.297789485740465No Hit
TATGCTGGGTGAACGTAGGAAACCTGTCGATGCTTACATCTTACTGAGTCTG520.297789485740465No Hit
CACTATGGGACCCAAACTGTATTTTACCAATAAATATGCATATGATCTTTAA510.2920627648608407No Hit
CGGAATGGGATAGGTTAAAATCTGGGAGTGAGGGGACATGGACCAAAGTAAT500.2863360439812163No Hit
CTAGTAAGGGAAGCCATTTTGAAAGAACTATGCGTCAACCAATCGTACGCCG490.28060932310159203No Hit
TAGCTAGGGGCCTCACATAGTTAAGCAATCAATAAACAATAATAAAAATAAT480.2748826022219677No Hit
CTATGTGGGAAAGCCTTTAGTAAATTTTTTAACCTTAGACAACATGAGAGAA480.2748826022219677No Hit
TTTACAGGGGGGTCAAAATTTGCAAAATAAAGTACTGTTTTGGTGTGGGAGT470.26915588134234336No Hit
GCTATTGGGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAATTACAAACT470.26915588134234336No Hit
GCGAAACGGGATTTTAGGATAATTGCCTACAGAGGGATTTATTTTTATGATG470.26915588134234336No Hit
CCATAAGGGGGCTCAGGCTAGTTCCAAATTCCAAAAGATTGGCTTGTAAACC460.263429160462719No Hit
GCGTTGGGGCCAAAGCCAATCACTGGGGAGACCGAGCACAGGGAGGACCAAG460.263429160462719No Hit
GTACATGGGATCACTAATCATCTAAACAACTCACTGGAAATCCAATTAACAA450.25770243958309474No Hit
TACTGGGCTGCGGCAACCATGAAACCGCATCCAACAGTCCAAAAGCAGTCTT450.25770243958309474No Hit
ACTGATGGGGAAGTGGTGACATTTTGCATGATGAATGACCTGACTTTTAGCC430.24624899782384607No Hit
ATAATTGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCT430.24624899782384607No Hit
CAGAATGGGATAGGTTAAAATCTGGGAGTGAGGGGACATGGACCAAAGTAAT430.24624899782384607No Hit
TATAGTGGCTCTCTGCATGGTTTAAAAAGCCATTGGTTTTGTGTGTGTTTAC410.23479555606459743No Hit
CAACCCGGGAGTCCCTTCTGCAGTTTTCTCCAAGCTGTGTCCTGTGAATTTC400.22906883518497306No Hit
GCCTGGGGGATGCCACTAATGGCTATATTCTACATCTTGATGGCTTTTAGTA400.22906883518497306No Hit
TGCACAGGGGTCCATTTGGAGTTTCTTTACTTATTTGTGGTTGGAATGAGGG390.22334211430534875No Hit
GATACTGGGTTAGCACAAATTCCAAAATGTGAAGCATAGCTATACTTCATAT390.22334211430534875No Hit
ACAGCAGGGAAACAGTTTTACTTGTGTCATATTTTAAAATTACTAACTGCCA380.21761539342572442No Hit
GCCACAGGGATGATTCTTTTAAACATTAAGTTTGAAATAGGAATGGTTTTCT380.21761539342572442No Hit
CCAGTGGATAATAGATACAACGATAGCTTATCCCAAAGCTGAACCTATAGAT380.21761539342572442No Hit
GTCACTGGGCAGATGGTGGCGTCTTTCAGGTAAGAAATCCTAGGAATCTTGT370.2118886725461001No Hit
TAGGCAGGGACCTGAAGAGATTTTTTGAAGTCCGGAGAGTTGTTTAAAAAGC370.2118886725461001No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAG360.20616195166647577No Hit
TTGTTGGGGTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT360.20616195166647577No Hit
CCGATAGGGATTTCAGATTTTGATAACTGTTTATATGTGTTGAAAACCAAAA350.20043523078685146No Hit
AACAGTGGGACCACTATGTATTTCTTCCATGAAGGCATTTGGATCATTCTAC340.19470850990722713No Hit
TGCAGTGGGAATGTGGTTGCCCTGTCCACTACATGGTTCTATCAGTAGTGTA330.1889817890276028No Hit
GGCTCAGGTGAAAATGTTCATCAATTCCCATTGCATCACCTCTGTAATTTTT320.18325506814797848No Hit
GCCAAGGGTGGGCCGACTTTACAGACGTCCAGAGCAAAGCCCCTTCTTTGTA320.18325506814797848No Hit
TTTTGCGGGATGAGGATGCTGAGGTTGACGACAGGTTCCAGGTCCTTTCACT310.17752834726835415No Hit
GTAGCTGGGACTAACGGCTTCTCTGATATTTATTGTAAGTTTTAGTACTGAT310.17752834726835415No Hit
ATAAATGGGAGTCCACCCTAAGTACTGTGCACAGTATCTCCCTGTGTGTGTG300.1718016263887298No Hit
GACCTTGGGGAAGTGTGAACGTGATGTTTTCGGATGCAAACTCAGCTCAGGG300.1718016263887298No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCTTATAC300.1718016263887298No Hit
TATGTTGGGATCAAGTGGAGATTGTTTTTACTTTTAAGGGTATTGCTTTTGT300.1718016263887298No Hit
TGCTGTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG300.1718016263887298No Hit
AAGCAGGGGAAGGCGCACGTGTACCTGCTCTCAGCGGCAGTGCGGCCTCCCC290.16607490550910547No Hit
AAGAACGGGATATCAGTCCTTTGTGGGTAGTAGGTAGCAAGTAAGAAACTTT290.16607490550910547No Hit
TTGAGTGGGCTCAGTTGAGTGGTTCACACTCAGACTTTGGCTTTATGGTTTT280.16034818462948114No Hit
ATTGATGGGATATCATCTAGAGTCCTTACTGGTATTCAGGATGAAAATGTTC280.16034818462948114No Hit
GCATCGGTGAATGGGAGAGAGTCTGTAGAGAATTGATTCAGAAAAGTGTCTG280.16034818462948114No Hit
GTATGCGGGACACGACGTCAAAAAGTGACGTTCATGCTAAGTGTTTTTCCAG270.15462146374985683No Hit
ACCACAGGGAGTAGAACAGATAATCAGTGTGTCTAAATTTGAATGTTAAGCA260.1488947428702325No Hit
GGCTTAGGGGCTACACTGGAAGGCCGCTAGGAAGCCCTTGCTTCTCTCAACA260.1488947428702325No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCT260.1488947428702325No Hit
GATTAAGGGCACCCCATACATAATATACACAGTTTGTATAAATGCATACATT250.14316802199060816No Hit
TTGTATGGGTCAGTACCGACGTACACTTCTGACCATTTTGTATGTGTCCTTG240.13744130111098385No Hit
TCGGACGGGAAGGCAAAACACAAAGGAGTGTGGCAGGTACCACGTAGACCCA240.13744130111098385No Hit
TGGCACGGGGGACTTCGAAATTGCTACAAAGGAGGATCCTCGCAGCTTCTAC240.13744130111098385No Hit
TAAGCAAGGGCAATTTACTAGGTTTTTCTGCTTTGGACATAATTGTATGTGT230.1317145802313595No Hit
ATAGAGGGGAAGCTAAATTTTTGGTGATTGACATAAAGATTTACTTGCTCAT230.1317145802313595No Hit
ACTTTTGGGGTTTACCACATGTACAAAAACTCTTTGAATAATATGCATTCCT220.12598785935173518No Hit
TGACCCGGGATCTGAAACGGGGTCACCTTTTCTAAAAAGTCAATTCATCTAG220.12598785935173518No Hit
GAACTAGGGACAAATTCATATTCTGTTACTACAGTAAACGTTAAGAGTTTTA220.12598785935173518No Hit
TCCCATGGGACGGCTTCATCTTCTACCTGCTCGCCTCCGTCCTGCTCTCCCT210.12026113847211087No Hit
AAGTGGGCTTACCATACACATTATCTCCAGAGGTTATTTCTACCCCACTCCC200.11453441759248653No Hit
TCATGGGGACATCTATTCCATGCTGTACTACTCAGCAGTAAAAAGGAGCAAA200.11453441759248653No Hit
GTATGCGGGTCTCCCCTCACAGTTTCTTGTTTCGAGTGCTGACTGACCCGTT200.11453441759248653No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGT200.11453441759248653No Hit
TGCTATGGGTCTGCATATGGGGATGGGGCATGGTGGCTCACGCCTGTAATCC190.10880769671286221No Hit
AGTGCTGGGAGGAGAGTTTGAGTTTTTTCCTGCTTCTAAGATGGTACAGAGC190.10880769671286221No Hit
CTACATGGGGCAGCGGCGCAGAGGAGGTCAGCTGCGGGAGCGTTTCCGGGGA180.10308097583323789No Hit
GAGGCTGGTATTTTTATGGGGGTTCACTATAAATAATTTAACCTATTATGAA180.10308097583323789No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)