FastQCFastQC Report
Sat 31 Dec 2022
SRR5844006.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844006.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17165
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC1410.8214389746577337No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT1280.7457034663559569RNA PCR Primer, Index 17 (95% over 22bp)
TAAACCGGGCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGC1280.7457034663559569No Hit
TAAAAGGGGGACGGCCTCGCCTCGGTATCGCAGCGGGTCCTCTCTATCTAGC1240.7224002330323332No Hit
AATTCGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1210.7049228080396155No Hit
GGTAATGGGGGGCCCGGCGGCGATGGCGCGGAGCGTGCGCGTGCTGGTGGAC1210.7049228080396155No Hit
CCTTTGGGGGCATGCGCGGGGGCCATATTATCAGCGGTTATTCGGTGAGCGG1080.6291872997378386No Hit
TATTTCGGGGGATGAGTGAGTTATATAAACTTACTTCCATTCTGTTTCGGAT1080.6291872997378386No Hit
TTGCATGGGAGATATATGTGTTCATGTATTTTCTGAAATTGCTTTCATAGAA1040.605884066414215No Hit
CTAGTAGGGACATACACAGTCTCAGCAAGGTTATAAAGAACCCTGTCAGGTC1040.605884066414215No Hit
CGCTATGGGGCGTACCTAGGATGCCGCGTGGAAGCCGAAGCCGCACCTCCCG1030.600058258083309No Hit
CTTATTGGGGGAGTTTGACGCCGAGTTCCAAAGGCACTACCTGCAGAAGATG1020.5942324497524031No Hit
CACCATGGGGGATTAGAGGCAATAGATCTGACTTGTGGCAAACTTAGTGTTT970.5651034080978736No Hit
TCACGAGGGCTGCAGGGTGACCACTGTGACAGGCTGGGAAGCATAGCCTTTT950.5534517914360617No Hit
TCATTCGGGGCTGAAGGATTGGTTTGGAGCTCTCCACGAGGATGCGAACAGA950.5534517914360617No Hit
AGTAACGGGATCTGTGACAGTCTCTATTCTGTCGTTTTGCTCCTTGGTATTT930.5418001747742499No Hit
TTGACAGGGACCTGTCCCCCTGCCTGTCTCCAGTGGGAAGGTAGCCTGGCCA930.5418001747742499No Hit
TACTTAGGGAGACTCCTTCTGTCCAGGAGCGCAGCAGTAGCTGCAGCAGTCA920.535974366443344No Hit
AAACATGGGGAAATTTGACAGATCACTCAAAATTTGCATGTGAAGAATATAA920.535974366443344No Hit
AGCAAAGGGGCCGCCTCGCGGACCCCGGAAGCGCGCGTGGCCGCCATGGCGC910.5301485581124381No Hit
TGCTGAGGGACCTCTTGCTACACCCATTCTTTTCATGTGCAGCCGACTCAAA880.5126711331197203No Hit
AAACTAGGGAATCTTTAATGTGTATGCACCATTACAGCATTAAAACACATGA880.5126711331197203No Hit
GTTTATGGGAGCAGTTAAACCACTAATAAGGTTTATTGTAAAATGCATGGCA880.5126711331197203No Hit
ATTATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA860.5010195164579085No Hit
TGTGCTGGGCTCTGCAGAGTGCCCCATGCAGCTTGAGGACTTCCCGATGGAT840.4893678997960967No Hit
TGGTACGGGGAAGATAGAAGAAAAAAGAATGTCAGAAAAACAATAAGAGCGT840.4893678997960967No Hit
AAGTTAGGGTCTTCACACACACAGACACACACTCACACATACACACCCAAAG840.4893678997960967No Hit
ATGCTAGGGGAGACTTACAATAACAAGTTGAGTGGCAAGCCTGGTATGCTGT840.4893678997960967No Hit
GGTGCAGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG840.4893678997960967No Hit
AAGCCGGGGGCAGGTGTGCTGCTGAGGCGTGAGAATGGCGTCCCGCGGCCGG830.4835420914651908No Hit
CAGACTGGGACCCTGCTGAAGCTCTTTGTCAAATAGGTAGTAGTTAGAACTG820.4777162831342848No Hit
ACATGTGGGACTGCGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTA820.4777162831342848No Hit
GCTCCCGGGTCAGGAAGGTATCACACACTTTCTGACTGCAACTTCTGTCCCT810.471890474803379No Hit
AGCTTCGGGCAGCCGCAGCTACCCAGAAATCCTGACACTCAAGACATACCTG790.4602388581415672No Hit
TAACGAGGGGATGTGGTTATAGCTGAAAAGCTGGCCTTGGAACAGCAAGTAG770.4485872414797553No Hit
GCCTCAGGGAGGGAGATGCGTGGGCTGACATCTGCAGGCCGAAAGAGCCGTG760.44276143314884936No Hit
TGCCCCCAATCCTGCTCCTCCTCTCATGTTTCCTCTTTGACTAAATGGCATT760.44276143314884936No Hit
CTACAAGGGGCTTGAGTTACACCTTGTGACCCTTCTCCCATAACATGGTGTG740.43110981648703756No Hit
ATTGATGGGGAATTGTTCCAGACATACCAACTGAACTTTCATTCATTATCAA740.43110981648703756No Hit
GATACGGGGCAGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCGC730.42528400815613165No Hit
GTGCCGGGGCGATCTTATATTGGGGGACTTACGTTTATTGCAAAATGAGTTT730.42528400815613165No Hit
AGAATGGGGGACGCTCTTCCGATCTGGCGTGGGGGTATATTTTAGCAAAAGG720.4194581998252257No Hit
TGAAACGGGTAATTTAGGAACAAAGCTTTGTCCTACTGACTAGGAAGTAAAC710.41363239149431985No Hit
GATTAAGGGGCTTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGGCG710.41363239149431985No Hit
AGGCGTGGGCACCGCGGTGGTGCTTGTCTCTGCAGACCGCTCGGGCATGGAG700.40780658316341395No Hit
TACGGTGGGATGGCAGTAAGCCTCATGTATACTAAGGAGAGTCTTCCAGGTG700.40780658316341395No Hit
TAATGCGGGGGAAATGGCCAGGGCCGAGCAGTTAGCACAGCAAGCTCACAGC700.40780658316341395No Hit
TATCCAAGGGATGACCTTTAAAATATGTTGCTTGATTTTCTTTCCCCTGAAA690.401980774832508No Hit
CAGACTTGGGACAGCCGCGCGCTGCAGGTGGTGCGCGCCCGCAAGCAGATCG690.401980774832508No Hit
TTCACCGGGACTGTAACCTCCACCTCCTGGGTTCACGCAATTCTCCTGCCTC690.401980774832508No Hit
AGTTACGGAAGTTCATAAGATGAGAAAAATCTACGAGCTTCTTATTTACAAC690.401980774832508No Hit
ATTCATGGGAGTTTGTACAGGCAAGTCCTGGGCTGGGTAAAAAGTTATATTA680.39615496650160215No Hit
TATTAAGGGATTTTAAACTGTTTTTTTCCTATTCTGCTTTTTGCTGCTCTCA670.3903291581706962No Hit
TGCCAAGGGATATGTATTAACATTTCTCTGATCAAAATTGTGGCTGTTTTCC670.3903291581706962No Hit
CGCAGCGGGAGAGTCTCAATCAAACCATAGGTGGGACAGATACCATTATCTC660.3845033498397903No Hit
ATCATAGGGATTCCGTTATGGGGAAAAATTTCATTAATTTTCTCAAATTTAC660.3845033498397903No Hit
TTTTACGGGCAGTACTAAAACCATACGAATTATTGGTTTATTCCAGAAAATA660.3845033498397903No Hit
CAGCATGGGTGGACAGAGGCATTTGACTAAGCCAACCTCCTCTCACAGCCTC650.37867754150888433No Hit
TAAAATGGGCATGTTTCTGAGAGCTTTAACTACTTCCTTATTTTTATGCAAT650.37867754150888433No Hit
ACTTATGGGATAACAATGGGTTCTAAAATCATCAGCATTTTTATGTATTGAG640.37285173317797843No Hit
CTACGTGGGCGATCTGGCGTAGGGAATGAAGGCTAGCTAGAGCATTAGCTGG630.3670259248470725No Hit
AAGGCAGGGCTAGCCACCAGCCCTGTCCTGCCTTGAGCACCTCCACCTGGGA630.3670259248470725No Hit
TTAGTAGGGATCGACTAAAACTGGCAGAAATTGTGAAGCAGGTGATAGAAGA620.3612001165161666No Hit
TATGCTGGGTGAACGTAGGAAACCTGTCGATGCTTACATCTTACTGAGTCTG620.3612001165161666No Hit
TAATTAGGGAGGGTCCTGAGATTCATCGCCCAGAATCAGAACCGAGACCCCC610.3553743081852607No Hit
TACTCAGGGGATATTAACAGTTGTGCTTATGTAACATCTGTGTTGTGTGTGT610.3553743081852607No Hit
GTTAGAGGGCTGGGCAAGTCCCTCTGCTCAAGATCACTTTATGGGGCAGCTG600.34954849985435477No Hit
ATGCTAGGGTGGTGATACAGTCAGTATATAGTAACCTCTCAGGTTTTTGAGA600.34954849985435477No Hit
CTAGTAAGGGAAGCCATTTTGAAAGAACTATGCGTCAACCAATCGTACGCCG600.34954849985435477No Hit
TACCTAGGGTGGAAGGGTTGAGGATGGACCGGAGAATTGGAACTTCAGAATA600.34954849985435477No Hit
TAGCTAGGGGCCTCACATAGTTAAGCAATCAATAAACAATAATAAAAATAAT590.34372269152344886No Hit
ATTGGCGGGTAGCCCTCTCCTTTTAACAAACTCCTTTCTCCATCTGTTCAAA590.34372269152344886No Hit
CTATAAGGGAAGGGTGACGACTTACTCCCGGCAGGGACTGAGGATTACATTC590.34372269152344886No Hit
ATAGAAGGGCGATCTGAAACGGGCCACTGTTCCCCGTGCAGACAAAATACAT580.33789688319254296No Hit
GCGGTTGGGAGGAGCAAAAAAGCATTTGACAAAATCCAACACCCTTTCATGG560.3262452665307311No Hit
GATATGGGGAAAATGAAACCAAAAGCCCAGTCCCATTTACAGTAGGAAATAA560.3262452665307311No Hit
GATACTGGGTTAGCACAAATTCCAAAATGTGAAGCATAGCTATACTTCATAT560.3262452665307311No Hit
GCTTTAGGGGTGGTATGGCAGAATTGAAGATGATGCCTAGATTTGAAGGGGA540.3145936498689193No Hit
TATAGTGGCTCTCTGCATGGTTTAAAAAGCCATTGGTTTTGTGTGTGTTTAC540.3145936498689193No Hit
CAACCCGGGAGTCCCTTCTGCAGTTTTCTCCAAGCTGTGTCCTGTGAATTTC530.3087678415380134No Hit
AAATCTGGGGGAAAAACGAATGCTCTTACACTGAATGTACAGGCAGTGGGCT530.3087678415380134No Hit
TTGACAAGGATGGCAATGGTTATATCAGTGCAGCAGAACTACGTCACGTCAT510.29711622487620154No Hit
GAAAGGGCATGGCACAGAGAAATTATATTCCTTACTTCATGTCAGTTTATGT500.29129041654529564No Hit
ACTGATGGGGAAGTGGTGACATTTTGCATGATGAATGACCTGACTTTTAGCC500.29129041654529564No Hit
CGCGGTGGGCATATCTTTTACTGTACTCTGTATTTTTATTATGCAAGGAAGA500.29129041654529564No Hit
CCAGTGGATAATAGATACAACGATAGCTTATCCCAAAGCTGAACCTATAGAT500.29129041654529564No Hit
TTTACAGGGGGGTCAAAATTTGCAAAATAAAGTACTGTTTTGGTGTGGGAGT490.28546460821438974No Hit
CAGAATGGGATAGGTTAAAATCTGGGAGTGAGGGGACATGGACCAAAGTAAT490.28546460821438974No Hit
GCGTTGGGGCCAAAGCCAATCACTGGGGAGACCGAGCACAGGGAGGACCAAG490.28546460821438974No Hit
TTAATGGGGACTCATTTTATTCATTAGTCTAAGATAAGAATTGTAACACTTC480.27963879988348384No Hit
GCCACAGGGATGATTCTTTTAAACATTAAGTTTGAAATAGGAATGGTTTTCT470.2738129915525779No Hit
CGGAATGGGATAGGTTAAAATCTGGGAGTGAGGGGACATGGACCAAAGTAAT470.2738129915525779No Hit
CACGAGGGTCCGATCTTAAGCAGGGTTATTTAAAAATCAGTTTTAAAAAATG460.267987183221672No Hit
GCGAAACGGGATTTTAGGATAATTGCCTACAGAGGGATTTATTTTTATGATG450.2621613748907661No Hit
CCATAAGGGGGCTCAGGCTAGTTCCAAATTCCAAAAGATTGGCTTGTAAACC440.2563355665598602No Hit
ACGTTTGGGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCAT430.25050975822895427No Hit
ACAGCAGGGAAACAGTTTTACTTGTGTCATATTTTAAAATTACTAACTGCCA420.24468394989804834No Hit
GTACATGGGATCACTAATCATCTAAACAACTCACTGGAAATCCAATTAACAA420.24468394989804834No Hit
CCGATAGGGATTTCAGATTTTGATAACTGTTTATATGTGTTGAAAACCAAAA420.24468394989804834No Hit
GCATCGGTGAATGGGAGAGAGTCTGTAGAGAATTGATTCAGAAAAGTGTCTG420.24468394989804834No Hit
AACAGTGGGACCACTATGTATTTCTTCCATGAAGGCATTTGGATCATTCTAC410.2388581415671424No Hit
GCTGTGGGTTACAGGCATGAGCCACCACAGCTGGCCCCCTTCTGTTTTATGT400.2330323332362365No Hit
CTATGTGGGAAAGCCTTTAGTAAATTTTTTAACCTTAGACAACATGAGAGAA400.2330323332362365No Hit
GTAGCTGGGACTAACGGCTTCTCTGATATTTATTGTAAGTTTTAGTACTGAT390.2272065249053306No Hit
TTTTGCGGGATGAGGATGCTGAGGTTGACGACAGGTTCCAGGTCCTTTCACT380.22138071657442468No Hit
CACTATGGGACCCAAACTGTATTTTACCAATAAATATGCATATGATCTTTAA380.22138071657442468No Hit
CTTTTGGAGGGCAGTAGTGCTAATGCATTTTGCACTAGAACGCTTCGGGAAA380.22138071657442468No Hit
ATAATTGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCT380.22138071657442468No Hit
TAGGCAGGGACCTGAAGAGATTTTTTGAAGTCCGGAGAGTTGTTTAAAAAGC380.22138071657442468No Hit
TACTGGGCTGCGGCAACCATGAAACCGCATCCAACAGTCCAAAAGCAGTCTT360.20972909991261285No Hit
TGCACAGGGGTCCATTTGGAGTTTCTTTACTTATTTGTGGTTGGAATGAGGG350.20390329158170697No Hit
TTGTTGGGGTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT350.20390329158170697No Hit
GCTATTGGGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAATTACAAACT340.19807748325080107No Hit
GCCTGGGGGATGCCACTAATGGCTATATTCTACATCTTGATGGCTTTTAGTA320.18642586658898921No Hit
GCCAAGGGTGGGCCGACTTTACAGACGTCCAGAGCAAAGCCCCTTCTTTGTA320.18642586658898921No Hit
AAGCAGGGGAAGGCGCACGTGTACCTGCTCTCAGCGGCAGTGCGGCCTCCCC310.1806000582580833No Hit
TTGTATGGGTCAGTACCGACGTACACTTCTGACCATTTTGTATGTGTCCTTG310.1806000582580833No Hit
GTATGCGGGACACGACGTCAAAAAGTGACGTTCATGCTAAGTGTTTTTCCAG290.16894844159627148No Hit
GACCTTGGGGAAGTGTGAACGTGATGTTTTCGGATGCAAACTCAGCTCAGGG280.16312263326536555No Hit
TCGGACGGGAAGGCAAAACACAAAGGAGTGTGGCAGGTACCACGTAGACCCA280.16312263326536555No Hit
GAACTAGGGACAAATTCATATTCTGTTACTACAGTAAACGTTAAGAGTTTTA280.16312263326536555No Hit
GGCTCAGGTGAAAATGTTCATCAATTCCCATTGCATCACCTCTGTAATTTTT270.15729682493445965No Hit
TGGCACGGGGGACTTCGAAATTGCTACAAAGGAGGATCCTCGCAGCTTCTAC270.15729682493445965No Hit
ATAGAGGGGAAGCTAAATTTTTGGTGATTGACATAAAGATTTACTTGCTCAT260.15147101660355375No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAG260.15147101660355375No Hit
AAGTGGGCTTACCATACACATTATCTCCAGAGGTTATTTCTACCCCACTCCC250.14564520827264782No Hit
GAGGCTGGTATTTTTATGGGGGTTCACTATAAATAATTTAACCTATTATGAA250.14564520827264782No Hit
TGCAGTGGGAATGTGGTTGCCCTGTCCACTACATGGTTCTATCAGTAGTGTA240.13981939994174192No Hit
GGCTTAGGGGCTACACTGGAAGGCCGCTAGGAAGCCCTTGCTTCTCTCAACA240.13981939994174192No Hit
TATGTTGGGATCAAGTGGAGATTGTTTTTACTTTTAAGGGTATTGCTTTTGT240.13981939994174192No Hit
GCGAAACGGGATTTTAGGATAATTGCCTACAGAGGGATTTATTTTTACGATG240.13981939994174192No Hit
TCCACTGGTCTTAAATGCAGGTTAAAATCTCTGTGAGGCAAAGGGATAATAT230.133993591610836No Hit
TGCTGTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG230.133993591610836No Hit
CTACATGGGGCAGCGGCGCAGAGGAGGTCAGCTGCGGGAGCGTTTCCGGGGA220.1281677832799301No Hit
GTCACTGGGCAGATGGTGGCGTCTTTCAGGTAAGAAATCCTAGGAATCTTGT220.1281677832799301No Hit
ACTTTTGGGGTTTACCACATGTACAAAAACTCTTTGAATAATATGCATTCCT220.1281677832799301No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCTTATAC210.12234197494902417No Hit
CGGGACGGGCTTATTGGCCTGTAACTTATTTACTAAATGCTCAGTGCTATTT210.12234197494902417No Hit
AACAATGGGAGTAGAACTTATTGACCTTGGGGACCCGGATGCAGCAGATGTG200.11651616661811826No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCT200.11651616661811826No Hit
TTGAGTGGGCTCAGTTGAGTGGTTCACACTCAGACTTTGGCTTTATGGTTTT200.11651616661811826No Hit
TCCCATGGGACGGCTTCATCTTCTACCTGCTCGCCTCCGTCCTGCTCTCCCT200.11651616661811826No Hit
GATTAAGGGCACCCCATACATAATATACACAGTTTGTATAAATGCATACATT190.11069035828721234No Hit
TATCCAGGGATGCCTTAGTAAAATTCATATCAAGTTACACTTCCTTGATTTT180.10486454995630642No Hit
TTGTTTGGGGGATCTGAGTATGGGATGCTATCATAGGCAGAAAGAAAATTGA180.10486454995630642No Hit
AAATAGGGGATGATTTGCAAATGAGGAGAGAGAAGATGAGGTTGGACAAGAT180.10486454995630642No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)