FastQCFastQC Report
Sat 31 Dec 2022
SRR5844005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17340
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC1340.7727797001153403No Hit
TAAACCGGGCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGC1300.7497116493656286No Hit
TAAAAGGGGGACGGCCTCGCCTCGGTATCGCAGCGGGTCCTCTCTATCTAGC1230.7093425605536332No Hit
CGCTATGGGGCGTACCTAGGATGCCGCGTGGAAGCCGAAGCCGCACCTCCCG1210.6978085351787774No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT1170.6747404844290658RNA PCR Primer, Index 17 (95% over 22bp)
AATTCGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1170.6747404844290658No Hit
ATTGATGGGGAATTGTTCCAGACATACCAACTGAACTTTCATTCATTATCAA1080.6228373702422145No Hit
TTGCATGGGAGATATATGTGTTCATGTATTTTCTGAAATTGCTTTCATAGAA1030.594002306805075No Hit
TCATTCGGGGCTGAAGGATTGGTTTGGAGCTCTCCACGAGGATGCGAACAGA1030.594002306805075No Hit
ACATGTGGGACTGCGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTA1030.594002306805075No Hit
CACCATGGGGGATTAGAGGCAATAGATCTGACTTGTGGCAAACTTAGTGTTT1000.5767012687427913No Hit
TCACGAGGGCTGCAGGGTGACCACTGTGACAGGCTGGGAAGCATAGCCTTTT1000.5767012687427913No Hit
CTAGTAGGGACATACACAGTCTCAGCAAGGTTATAAAGAACCCTGTCAGGTC980.5651672433679354No Hit
CCTTTGGGGGCATGCGCGGGGGCCATATTATCAGCGGTTATTCGGTGAGCGG970.5594002306805075No Hit
CTACGTGGGCGATCTGGCGTAGGGAATGAAGGCTAGCTAGAGCATTAGCTGG950.5478662053056517No Hit
AAACTAGGGAATCTTTAATGTGTATGCACCATTACAGCATTAAAACACATGA900.5190311418685121No Hit
AAGTTAGGGTCTTCACACACACAGACACACACTCACACATACACACCCAAAG890.5132641291810842No Hit
CTTATTGGGGGAGTTTGACGCCGAGTTCCAAAGGCACTACCTGCAGAAGATG880.5074971164936563No Hit
TTTTACGGGCAGTACTAAAACCATACGAATTATTGGTTTATTCCAGAAAATA880.5074971164936563No Hit
AAGCCGGGGGCAGGTGTGCTGCTGAGGCGTGAGAATGGCGTCCCGCGGCCGG870.5017301038062283No Hit
TAATTAGGGAGGGTCCTGAGATTCATCGCCCAGAATCAGAACCGAGACCCCC860.4959630911188005No Hit
GCCTCAGGGAGGGAGATGCGTGGGCTGACATCTGCAGGCCGAAAGAGCCGTG860.4959630911188005No Hit
TGCCCCCAATCCTGCTCCTCCTCTCATGTTTCCTCTTTGACTAAATGGCATT860.4959630911188005No Hit
AAACATGGGGAAATTTGACAGATCACTCAAAATTTGCATGTGAAGAATATAA860.4959630911188005No Hit
GAAAGGGCATGGCACAGAGAAATTATATTCCTTACTTCATGTCAGTTTATGT850.49019607843137253No Hit
TATTAAGGGATTTTAAACTGTTTTTTTCCTATTCTGCTTTTTGCTGCTCTCA840.4844290657439446No Hit
CTACAAGGGGCTTGAGTTACACCTTGTGACCCTTCTCCCATAACATGGTGTG830.47866205305651677No Hit
ATGCTAGGGGAGACTTACAATAACAAGTTGAGTGGCAAGCCTGGTATGCTGT830.47866205305651677No Hit
GGTGCAGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG830.47866205305651677No Hit
ATTATGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA820.4728950403690888No Hit
CAGACTTGGGACAGCCGCGCGCTGCAGGTGGTGCGCGCCCGCAAGCAGATCG800.461361014994233No Hit
ATGCTAGGGTGGTGATACAGTCAGTATATAGTAACCTCTCAGGTTTTTGAGA790.4555940023068051No Hit
AGCAAAGGGGCCGCCTCGCGGACCCCGGAAGCGCGCGTGGCCGCCATGGCGC790.4555940023068051No Hit
GATTAAGGGGCTTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGGCG780.4498269896193772No Hit
GGTAATGGGGGGCCCGGCGGCGATGGCGCGGAGCGTGCGCGTGCTGGTGGAC780.4498269896193772No Hit
GTTTATGGGAGCAGTTAAACCACTAATAAGGTTTATTGTAAAATGCATGGCA770.44405997693194926No Hit
TATCCAAGGGATGACCTTTAAAATATGTTGCTTGATTTTCTTTCCCCTGAAA760.4382929642445213No Hit
TAACGAGGGGATGTGGTTATAGCTGAAAAGCTGGCCTTGGAACAGCAAGTAG750.43252595155709345No Hit
TTCACCGGGACTGTAACCTCCACCTCCTGGGTTCACGCAATTCTCCTGCCTC750.43252595155709345No Hit
TAATGCGGGGGAAATGGCCAGGGCCGAGCAGTTAGCACAGCAAGCTCACAGC750.43252595155709345No Hit
TTGACAGGGACCTGTCCCCCTGCCTGTCTCCAGTGGGAAGGTAGCCTGGCCA750.43252595155709345No Hit
AGGCGTGGGCACCGCGGTGGTGCTTGTCTCTGCAGACCGCTCGGGCATGGAG730.4209919261822376No Hit
TATTTCGGGGGATGAGTGAGTTATATAAACTTACTTCCATTCTGTTTCGGAT720.4152249134948097No Hit
ATCATAGGGATTCCGTTATGGGGAAAAATTTCATTAATTTTCTCAAATTTAC720.4152249134948097No Hit
CTAGTAAGGGAAGCCATTTTGAAAGAACTATGCGTCAACCAATCGTACGCCG710.40945790080738176No Hit
TACTTAGGGAGACTCCTTCTGTCCAGGAGCGCAGCAGTAGCTGCAGCAGTCA710.40945790080738176No Hit
TGAAACGGGTAATTTAGGAACAAAGCTTTGTCCTACTGACTAGGAAGTAAAC700.40369088811995385No Hit
GATACGGGGCAGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCGC700.40369088811995385No Hit
CTATAAGGGAAGGGTGACGACTTACTCCCGGCAGGGACTGAGGATTACATTC700.40369088811995385No Hit
CAGCATGGGTGGACAGAGGCATTTGACTAAGCCAACCTCCTCTCACAGCCTC690.397923875432526No Hit
AGTAACGGGATCTGTGACAGTCTCTATTCTGTCGTTTTGCTCCTTGGTATTT690.397923875432526No Hit
ATTCATGGGAGTTTGTACAGGCAAGTCCTGGGCTGGGTAAAAAGTTATATTA670.3863898500576701No Hit
GCTCCCGGGTCAGGAAGGTATCACACACTTTCTGACTGCAACTTCTGTCCCT670.3863898500576701No Hit
CAGACTGGGACCCTGCTGAAGCTCTTTGTCAAATAGGTAGTAGTTAGAACTG670.3863898500576701No Hit
TACCTAGGGTGGAAGGGTTGAGGATGGACCGGAGAATTGGAACTTCAGAATA670.3863898500576701No Hit
AGCTTCGGGCAGCCGCAGCTACCCAGAAATCCTGACACTCAAGACATACCTG660.3806228373702422No Hit
CACGAGGGTCCGATCTTAAGCAGGGTTATTTAAAAATCAGTTTTAAAAAATG660.3806228373702422No Hit
GATATGGGGAAAATGAAACCAAAAGCCCAGTCCCATTTACAGTAGGAAATAA650.3748558246828143No Hit
TAGCTAGGGGCCTCACATAGTTAAGCAATCAATAAACAATAATAAAAATAAT650.3748558246828143No Hit
TGCCAAGGGATATGTATTAACATTTCTCTGATCAAAATTGTGGCTGTTTTCC650.3748558246828143No Hit
GCTTTAGGGGTGGTATGGCAGAATTGAAGATGATGCCTAGATTTGAAGGGGA640.3690888119953864No Hit
TGGTACGGGGAAGATAGAAGAAAAAAGAATGTCAGAAAAACAATAAGAGCGT630.3633217993079585No Hit
CGCAGCGGGAGAGTCTCAATCAAACCATAGGTGGGACAGATACCATTATCTC620.35755478662053053No Hit
TTAATGGGGACTCATTTTATTCATTAGTCTAAGATAAGAATTGTAACACTTC620.35755478662053053No Hit
TATGCTGGGTGAACGTAGGAAACCTGTCGATGCTTACATCTTACTGAGTCTG620.35755478662053053No Hit
TACGGTGGGATGGCAGTAAGCCTCATGTATACTAAGGAGAGTCTTCCAGGTG610.3517877739331027No Hit
ATTGGCGGGTAGCCCTCTCCTTTTAACAAACTCCTTTCTCCATCTGTTCAAA610.3517877739331027No Hit
GCGGTTGGGAGGAGCAAAAAAGCATTTGACAAAATCCAACACCCTTTCATGG600.34602076124567477No Hit
TGTGCTGGGCTCTGCAGAGTGCCCCATGCAGCTTGAGGACTTCCCGATGGAT600.34602076124567477No Hit
TTAGTAGGGATCGACTAAAACTGGCAGAAATTGTGAAGCAGGTGATAGAAGA580.3344867358708189No Hit
ATAGAAGGGCGATCTGAAACGGGCCACTGTTCCCCGTGCAGACAAAATACAT580.3344867358708189No Hit
GTGCCGGGGCGATCTTATATTGGGGGACTTACGTTTATTGCAAAATGAGTTT580.3344867358708189No Hit
GCGAAACGGGATTTTAGGATAATTGCCTACAGAGGGATTTATTTTTATGATG580.3344867358708189No Hit
AGTTACGGAAGTTCATAAGATGAGAAAAATCTACGAGCTTCTTATTTACAAC580.3344867358708189No Hit
AAGGCAGGGCTAGCCACCAGCCCTGTCCTGCCTTGAGCACCTCCACCTGGGA570.328719723183391No Hit
GTTAGAGGGCTGGGCAAGTCCCTCTGCTCAAGATCACTTTATGGGGCAGCTG560.32295271049596314No Hit
TGCTGAGGGACCTCTTGCTACACCCATTCTTTTCATGTGCAGCCGACTCAAA550.31718569780853517No Hit
ACGTTTGGGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCAT540.31141868512110726No Hit
AGAATGGGGGACGCTCTTCCGATCTGGCGTGGGGGTATATTTTAGCAAAAGG540.31141868512110726No Hit
CCAGTGGATAATAGATACAACGATAGCTTATCCCAAAGCTGAACCTATAGAT540.31141868512110726No Hit
ACTTATGGGATAACAATGGGTTCTAAAATCATCAGCATTTTTATGTATTGAG530.30565167243367936No Hit
TACTGGGCTGCGGCAACCATGAAACCGCATCCAACAGTCCAAAAGCAGTCTT520.29988465974625145No Hit
AAATCTGGGGGAAAAACGAATGCTCTTACACTGAATGTACAGGCAGTGGGCT520.29988465974625145No Hit
GCCTGGGGGATGCCACTAATGGCTATATTCTACATCTTGATGGCTTTTAGTA520.29988465974625145No Hit
TAAAATGGGCATGTTTCTGAGAGCTTTAACTACTTCCTTATTTTTATGCAAT510.29411764705882354No Hit
TACTCAGGGGATATTAACAGTTGTGCTTATGTAACATCTGTGTTGTGTGTGT500.28835063437139563No Hit
CCATAAGGGGGCTCAGGCTAGTTCCAAATTCCAAAAGATTGGCTTGTAAACC500.28835063437139563No Hit
CGGAATGGGATAGGTTAAAATCTGGGAGTGAGGGGACATGGACCAAAGTAAT500.28835063437139563No Hit
CACTATGGGACCCAAACTGTATTTTACCAATAAATATGCATATGATCTTTAA490.2825836216839677No Hit
GATACTGGGTTAGCACAAATTCCAAAATGTGAAGCATAGCTATACTTCATAT490.2825836216839677No Hit
CGCGGTGGGCATATCTTTTACTGTACTCTGTATTTTTATTATGCAAGGAAGA490.2825836216839677No Hit
GCTGTGGGTTACAGGCATGAGCCACCACAGCTGGCCCCCTTCTGTTTTATGT480.27681660899653976No Hit
TTTACAGGGGGGTCAAAATTTGCAAAATAAAGTACTGTTTTGGTGTGGGAGT480.27681660899653976No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAG480.27681660899653976No Hit
GCGTTGGGGCCAAAGCCAATCACTGGGGAGACCGAGCACAGGGAGGACCAAG470.2710495963091119No Hit
ATAATTGGGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGCCCT460.265282583621684No Hit
TATAGTGGCTCTCTGCATGGTTTAAAAAGCCATTGGTTTTGTGTGTGTTTAC460.265282583621684No Hit
CTATGTGGGAAAGCCTTTAGTAAATTTTTTAACCTTAGACAACATGAGAGAA450.25951557093425603No Hit
TTGACAAGGATGGCAATGGTTATATCAGTGCAGCAGAACTACGTCACGTCAT450.25951557093425603No Hit
GCCACAGGGATGATTCTTTTAAACATTAAGTTTGAAATAGGAATGGTTTTCT430.24798154555940025No Hit
GCTATTGGGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAATTACAAACT430.24798154555940025No Hit
ACAGCAGGGAAACAGTTTTACTTGTGTCATATTTTAAAATTACTAACTGCCA420.2422145328719723No Hit
CAACCCGGGAGTCCCTTCTGCAGTTTTCTCCAAGCTGTGTCCTGTGAATTTC420.2422145328719723No Hit
CAGAATGGGATAGGTTAAAATCTGGGAGTGAGGGGACATGGACCAAAGTAAT420.2422145328719723No Hit
ACTGATGGGGAAGTGGTGACATTTTGCATGATGAATGACCTGACTTTTAGCC410.2364475201845444No Hit
AACAGTGGGACCACTATGTATTTCTTCCATGAAGGCATTTGGATCATTCTAC400.2306805074971165No Hit
TCGGACGGGAAGGCAAAACACAAAGGAGTGTGGCAGGTACCACGTAGACCCA400.2306805074971165No Hit
GTACATGGGATCACTAATCATCTAAACAACTCACTGGAAATCCAATTAACAA390.2249134948096886No Hit
CCGATAGGGATTTCAGATTTTGATAACTGTTTATATGTGTTGAAAACCAAAA390.2249134948096886No Hit
AAGCAGGGGAAGGCGCACGTGTACCTGCTCTCAGCGGCAGTGCGGCCTCCCC380.21914648212226065No Hit
TTGTTGGGGTTGGGGTTGTGTTGTCACCCAAGAGATTGTTGTTTGCCATACT380.21914648212226065No Hit
GATTAAGGGCACCCCATACATAATATACACAGTTTGTATAAATGCATACATT370.21337946943483277No Hit
CTTTTGGAGGGCAGTAGTGCTAATGCATTTTGCACTAGAACGCTTCGGGAAA370.21337946943483277No Hit
TGCACAGGGGTCCATTTGGAGTTTCTTTACTTATTTGTGGTTGGAATGAGGG360.20761245674740486No Hit
GGCTCAGGTGAAAATGTTCATCAATTCCCATTGCATCACCTCTGTAATTTTT350.20184544405997693No Hit
TATGTTGGGATCAAGTGGAGATTGTTTTTACTTTTAAGGGTATTGCTTTTGT330.1903114186851211No Hit
CTTCTAGGGTGCCATTTTGAGAAACACAGTCCAAACATGAGCATAAACAGAA330.1903114186851211No Hit
TAGGCAGGGACCTGAAGAGATTTTTTGAAGTCCGGAGAGTTGTTTAAAAAGC330.1903114186851211No Hit
TGGCACGGGGGACTTCGAAATTGCTACAAAGGAGGATCCTCGCAGCTTCTAC320.1845444059976932No Hit
GCCAAGGGTGGGCCGACTTTACAGACGTCCAGAGCAAAGCCCCTTCTTTGTA310.17877739331026526No Hit
GTCACTGGGCAGATGGTGGCGTCTTTCAGGTAAGAAATCCTAGGAATCTTGT300.17301038062283738No Hit
ACTTTTGGGGTTTACCACATGTACAAAAACTCTTTGAATAATATGCATTCCT300.17301038062283738No Hit
GGCTTAGGGGCTACACTGGAAGGCCGCTAGGAAGCCCTTGCTTCTCTCAACA300.17301038062283738No Hit
GTAGCTGGGACTAACGGCTTCTCTGATATTTATTGTAAGTTTTAGTACTGAT300.17301038062283738No Hit
GCATCGGTGAATGGGAGAGAGTCTGTAGAGAATTGATTCAGAAAAGTGTCTG290.16724336793540945No Hit
TCATGGGGACATCTATTCCATGCTGTACTACTCAGCAGTAAAAAGGAGCAAA280.16147635524798157No Hit
AAGAACGGGATATCAGTCCTTTGTGGGTAGTAGGTAGCAAGTAAGAAACTTT280.16147635524798157No Hit
TGCAGTGGGAATGTGGTTGCCCTGTCCACTACATGGTTCTATCAGTAGTGTA270.15570934256055363No Hit
GAGGCTGGTATTTTTATGGGGGTTCACTATAAATAATTTAACCTATTATGAA270.15570934256055363No Hit
GACCTTGGGGAAGTGTGAACGTGATGTTTTCGGATGCAAACTCAGCTCAGGG260.14994232987312572No Hit
TGCTGTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG260.14994232987312572No Hit
TTTTGCGGGATGAGGATGCTGAGGTTGACGACAGGTTCCAGGTCCTTTCACT250.14417531718569782No Hit
GTATGCGGGACACGACGTCAAAAAGTGACGTTCATGCTAAGTGTTTTTCCAG240.13840830449826988No Hit
ATAAATGGGAGTCCACCCTAAGTACTGTGCACAGTATCTCCCTGTGTGTGTG230.132641291810842No Hit
TTGAGTGGGCTCAGTTGAGTGGTTCACACTCAGACTTTGGCTTTATGGTTTT230.132641291810842No Hit
GAACTAGGGACAAATTCATATTCTGTTACTACAGTAAACGTTAAGAGTTTTA230.132641291810842No Hit
TCCCATGGGACGGCTTCATCTTCTACCTGCTCGCCTCCGTCCTGCTCTCCCT230.132641291810842No Hit
TCCACTGGTCTTAAATGCAGGTTAAAATCTCTGTGAGGCAAAGGGATAATAT220.12687427912341406No Hit
TTGTATGGGTCAGTACCGACGTACACTTCTGACCATTTTGTATGTGTCCTTG220.12687427912341406No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCTTATAC220.12687427912341406No Hit
ATTGATGGGATATCATCTAGAGTCCTTACTGGTATTCAGGATGAAAATGTTC220.12687427912341406No Hit
AGTGCTGGGAGGAGAGTTTGAGTTTTTTCCTGCTTCTAAGATGGTACAGAGC210.12110726643598616No Hit
ACCACAGGGAGTAGAACAGATAATCAGTGTGTCTAAATTTGAATGTTAAGCA200.11534025374855825No Hit
ACGTATGGGATGCATTATAAATGTTTTTAATTGTGTTCTGTTTTTTGCAGTC200.11534025374855825No Hit
GCGAAACGGGATTTTAGGATAATTGCCTACAGAGGGATTTATTTTTACGATG200.11534025374855825No Hit
ATAGAGGGGAAGCTAAATTTTTGGTGATTGACATAAAGATTTACTTGCTCAT200.11534025374855825No Hit
TGTATTGGGGTGGCAGTAAATACAACAAGACACTCTATGTATTAATGTCTCC200.11534025374855825No Hit
CTAGCTGGGACTTACAGGCACGTACCACTATACCCTGCTAAAATTTGTATTT190.10957324106113032No Hit
GCTTCAGGGTTGCTACTTAAATTTCCAATAATTAAAAATTTAAAATTTTTAA190.10957324106113032No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCT190.10957324106113032No Hit
ACGAATGGGAGAAAGGAAAACGCCGATAGAACGAATACCCTATTTAGGTTTT190.10957324106113032No Hit
TGACCCGGGATCTGAAACGGGGTCACCTTTTCTAAAAAGTCAATTCATCTAG180.10380622837370243No Hit
TTGTTTGGGGGATCTGAGTATGGGATGCTATCATAGGCAGAAAGAAAATTGA180.10380622837370243No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)