FastQCFastQC Report
Sat 31 Dec 2022
SRR5843992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165734
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC5970.36021576743456385No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT5930.35780226145510274RNA PCR Primer, Index 17 (95% over 22bp)
TGAAAAGGGAACACTCCAAGATGGGTTCATCTTTTCTTCCTTTCCCATTCAC2350.14179347629333752No Hit
GCTTTTGGGATCGATTGGAACCCTGATTGGCGGCGTCTACCCCGGGAGCTAA2170.1309326993857627No Hit
ACATCAGGGATCTGGATGGCGGTGGAAAAACTTGAGGGTTTGGGGTTCAAAG2090.12610568742684058No Hit
AGCTAGGGGTGATACAGCTGAATGAAGAAAGAATGCTGGAGAAAATGTAAGG2070.12489893443711007No Hit
AAGCACGGGCGATCTGTCAGTGGGCCCATTTGGTCCCGACAAGATGACATAG2060.1242955579422448No Hit
GTAAATGGGGGGAGAAAATATTTGCTAACTACTTATTTGATAGGGAATTAAA2010.12127867546791848No Hit
ACATCGGGGGTTAATAAAGATTTGGGAGTATTAGTGGTCTTAAAATGGACCA1980.11946854598332267No Hit
TACATGGGGACTAAGATCATCCTTGGCCCCCACCTTTATCCTTGTTAATGCT1980.11946854598332267No Hit
CTCTTAGGGCTTGGTTTTTAAATTACTTTACCTAATAAGGAACAATATTCCA1960.11826179299359214No Hit
AATACTGGGACAGAGAGGACATTGTGGGCTAATCATATGCTTATTTACACCT1960.11826179299359214No Hit
GCATCTTGGGATTCCATTCTCAAAATGACTTTGGAGTGAAAGTAATTATTTT1960.11826179299359214No Hit
AAATTAGGGATAGCCTTGAAAGTTCTTGGTTTGCCTTTACATTTTTATTTCT1940.11705504000386162No Hit
AAGCAGGGGGATAGTTTTATTTAGCACATCAGTGAATATATTCTCTATAGCA1940.11705504000386162No Hit
TTCCGAGGGGAGTCTTTGGAGGCACTCAAGCAGCAGCAAATGAAGCTCGTGA1940.11705504000386162No Hit
CTTACGGCGGGAGCGCACCGGGCCTGCCGAGGAGCGCGCGCCCGGCCGGGCT1930.11645166350899634No Hit
CAAATTGGGGGCGCCCGCCACTACGCCCGGCTAATTTTTTGTATTTTTAGTA1920.11584828701413108No Hit
CTAAAACGGGCACCTTATTTTTGGTCTTTGTTTATTCATTTAGACCCTGCAA1910.1152449105192658No Hit
AGCTCTGGGGGCGTGACGACGGCGCACTTACGGCAACCCACGCCTTCGACGT1910.1152449105192658No Hit
CTACTAAAAATACAGAAAATTGGCTGGGCGTGGTGGTACACGCCTGTAATCC1910.1152449105192658No Hit
TGCACAGGGAATGCCCTCCCCTGTTAAAGTAAATTCAAAAAATTGAAGGCAG1900.11464153402440055No Hit
AATTGCGGGATTTCCATTTTTAGGAGATCAAAGTTTGCAGGATTTGCAAAAG1890.11403815752953528No Hit
AATCGTGGGTCTTTTTGTATGTAGTTTTATGAAATTTCATACATGTATAGAT1850.11162465155007421No Hit
ACGCCTGGGGGTGAAGCGGGGGTGGGATCTGAACATGGCGGCGGTGGTAGCT1840.11102127505520894No Hit
TACACCGGGTCTTTTAGAGGGACCAAGATTTCTTAAGACAATCTACTTTACT1840.11102127505520894No Hit
CTTACTGGGGGACGATGTGCGGCGGATGAACAAGCGGCAGCTCTATTATCAA1820.10981452206547841No Hit
AGCCCAGGGGTTTGACCTGTGATCAAAATTGAACCATTGTACAGTTTGGCTT1820.10981452206547841No Hit
GATTATGGGTGTAGCTAAAAGTTCTCTTTTTCTTCTAGTTCTGTGTCCTACA1810.10921114557061315No Hit
GGGCAGGGGGCAAGCTTGCTTTTATTTTTGTCATATAAGTAATTTAAATCTT1800.1086077690757479No Hit
TCTACAGGGAGAGAAAAACAGAGCACTCATTTTGGGGAGTTAGCAAGAAATA1800.1086077690757479No Hit
TTAGCTAGGGGGGCGAGCGCCCGGGCGGCGGCGGGGGCGCCAGGAGAGTCCG1790.10800439258088262No Hit
CAAACTGGGACTAAAAGTGATGTGATTTATGCATTTATGCATGAGAACTAAA1790.10800439258088262No Hit
CATTCAGGGGAGGCTGCGGTGAGGCCTGGTCTCCGGCTGCCAGACCATGCTG1770.10679763959115209No Hit
TATACAGGGAAGTGAGTGAAGAATTTTTCATTACAGGAGGCTTTAATGCATA1770.10679763959115209No Hit
GCAATGGGCTCTTTGTGGCTCAGAAAATGTACAGGTTTTCCAGGGCAGCCTT1770.10679763959115209No Hit
GCTATTGGGATTATTAAATTTTTTATTGCTAAATATGTTTCTTTCATACGTT1770.10679763959115209No Hit
AAGGCAGGGTATGCTGCCCAGTTGGATGTGCATACAGCATCAAGTGCAAAGA1760.10619426309628682No Hit
TAACATGGGCGATCTGTACTGGGGTTGATAAGGAAGCAAGAACCAATTTTGT1760.10619426309628682No Hit
CTCCAGGGGATGGTCATGAACCTTTGCAGATAAGGAAACTGTGTTTCACAAG1760.10619426309628682No Hit
AGTCGCGGGTACTAATCGAATTATTTCTCATTTTAGTCAAGATGATTTTACT1740.10498751010655628No Hit
ATATTTGGGAGTGATTGCATGTTGAGAAATTCTCTGTCCCCTAACATCTTTA1740.10498751010655628No Hit
GATTTTGGGAGCCGAGGGCAGCATATGGTTACAAATTATATTTCAGAATTTC1720.10378075711682576No Hit
AGTGCCGGGGATTCTCGAGTTAGAGAGGTGATCAATAGAAATCTGTTGGATC1720.10378075711682576No Hit
TTCAGTGGGGACTGCTGGTTTACATTTCTGCTCTTTCTAATTCAGTTGTCTA1720.10378075711682576No Hit
TTGTTCGGGGTGTGTTAATATCGACGTACTTGGCATTCTAAGATGTCCTTTG1720.10378075711682576No Hit
AATCCGGGGATCTTTCGGGGGGAAGCCTTTGCAAGAAAATTGCATACAAGGC1720.10378075711682576No Hit
CTTTCAGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT1710.1031773806219605No Hit
GCCAATGGGTAATAAAGAAACAAATCTTAATTAAAATAAAGATACCGAGCAC1710.1031773806219605No Hit
TTAGACGGGATTAAGTAGCACTCTTGAGGAGTGTTCTCTTTGACTTCTAAGA1710.1031773806219605No Hit
AGCCTAGGGCTGGCACAAACCCGCATGTATGACCATCGGCCTTCAAAAACCC1700.10257400412709522No Hit
ATTGCAGGGCTTGTGGCTATTTTTAAGATTTTCTTTGTGTTTTGTTTTTCTC1700.10257400412709522No Hit
TCAACTGGGATTCCAGGAGGAAAGAGGGTTTTCATTCCCTTCTGAATGTTAG1700.10257400412709522No Hit
CCGCATGGGAGACTAAGAAAGGATGAAGAGGTAAGAGTAGAAAAATGACCAG1690.10197062763222997No Hit
GTGACTGGGAACTGTTTTCTTGCAGCAGCTTTATGATGAGAATCACTGTTTC1690.10197062763222997No Hit
GGTAACGGTGGAAAACTGATGTTATAAGAGGTGATCTAAATTGCCCAGTATT1680.1013672511373647No Hit
TGAGTAGGGGACTTCCTGGTGGGATCCGAGTGAGGCGACGGGGTAGGGGTTG1680.1013672511373647No Hit
CTTAGAGGGTACAACCAGAGATACAGGTTTTCCATCAAGAGGGGGCCTATAG1680.1013672511373647No Hit
ATTGCAGGGCACACTGACTGGGGGAGTTCAAAAGCAGAGGTGCATTCAGCAG1670.10076387464249942No Hit
ACAACCGGGAGAGTCTTCTCATTAACTTCCATGACAATTATTTCTCTGCATG1670.10076387464249942No Hit
TGGCTACGGGAAATCTTGATAGCAGGAGACAGCTTCCTGATCTAGATGTACA1660.10016049814763416No Hit
TGCCGCGGGAGTCAAGGTAAGGCTCTCTCCCCTAGAGCTAGCAAGCAGGTAA1660.10016049814763416No Hit
TAAATCGGGGGAGTGATGGCGCCGTCTATGTTCGACTAAACTAAAGTGTCAC1660.10016049814763416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)