FastQCFastQC Report
Sat 31 Dec 2022
SRR5843991.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843991.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165506
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT5810.3510446751175184RNA PCR Primer, Index 17 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC5650.3413773518784817No Hit
TGAAAAGGGAACACTCCAAGATGGGTTCATCTTTTCTTCCTTTCCCATTCAC2320.14017618696603146No Hit
TTCCGAGGGGAGTCTTTGGAGGCACTCAAGCAGCAGCAAATGAAGCTCGTGA2300.13896777156115186No Hit
AAGCACGGGCGATCTGTCAGTGGGCCCATTTGGTCCCGACAAGATGACATAG2160.1305088637269948No Hit
AGCTCTGGGGGCGTGACGACGGCGCACTTACGGCAACCCACGCCTTCGACGT2140.1293004483221152No Hit
CTTACGGCGGGAGCGCACCGGGCCTGCCGAGGAGCGCGCGCCCGGCCGGGCT2130.1286962406196754No Hit
ACATCAGGGATCTGGATGGCGGTGGAAAAACTTGAGGGTTTGGGGTTCAAAG2120.12809203291723564No Hit
TGCACAGGGAATGCCCTCCCCTGTTAAAGTAAATTCAAAAAATTGAAGGCAG2080.12567520210747646No Hit
CTACAGAGGGGCCCAAGAAAGAAATGAAGGCTAAATCACGTTCTAGGTCTGC2050.1238625790001571No Hit
CTCTTAGGGCTTGGTTTTTAAATTACTTTACCTAATAAGGAACAATATTCCA2040.1232583712977173No Hit
GCTTTTGGGATCGATTGGAACCCTGATTGGCGGCGTCTACCCCGGGAGCTAA2030.1226541635952775No Hit
GCAATGGGCTCTTTGTGGCTCAGAAAATGTACAGGTTTTCCAGGGCAGCCTT2020.12204995589283772No Hit
AGCCCAGGGGTTTGACCTGTGATCAAAATTGAACCATTGTACAGTTTGGCTT2020.12204995589283772No Hit
AATCGTGGGTCTTTTTGTATGTAGTTTTATGAAATTTCATACATGTATAGAT2010.12144574819039794No Hit
AGCTAGGGGTGATACAGCTGAATGAAGAAAGAATGCTGGAGAAAATGTAAGG2000.12084154048795814No Hit
AAATTAGGGATAGCCTTGAAAGTTCTTGGTTTGCCTTTACATTTTTATTTCT1990.12023733278551833No Hit
CTGTTCGGGAACCTGACATCATCTTTCCACCCGGCTTACCTCCACCAGATCT1980.11963312508307855No Hit
AAAGTGGGGGATCGGAATCGGACACTAACACAAAAGCTACGGATGGAGGAGC1980.11963312508307855No Hit
ATATTTGGGAGCATTTGGAAGACTCCCAGGTGTTCTCGTGAGAGAGGGAGAG1970.11902891738063877No Hit
GATTTTGGGAGCCGAGGGCAGCATATGGTTACAAATTATATTTCAGAATTTC1950.11782050197575919No Hit
TACATGGGGACTAAGATCATCCTTGGCCCCCACCTTTATCCTTGTTAATGCT1930.1166120865708796No Hit
TTAGCTAGGGGGGCGAGCGCCCGGGCGGCGGCGGGGGCGCCAGGAGAGTCCG1910.11540367116600002No Hit
CTTACTGGGGGACGATGTGCGGCGGATGAACAAGCGGCAGCTCTATTATCAA1910.11540367116600002No Hit
TAAAATGGGGTGCTCAGGGCAGGCCTCGCCTAGAAGATGATGTTTGAACAAA1910.11540367116600002No Hit
TATACAGGGAAGTGAGTGAAGAATTTTTCATTACAGGAGGCTTTAATGCATA1910.11540367116600002No Hit
GGGCTCACTGCAATCTCTGCCTCCCGGGTTCAAGTGGTTCTCCTGCCTCAGC1900.11479946346356024No Hit
ACATCGGGGGTTAATAAAGATTTGGGAGTATTAGTGGTCTTAAAATGGACCA1900.11479946346356024No Hit
CTTAGAGGGTACAACCAGAGATACAGGTTTTCCATCAAGAGGGGGCCTATAG1890.11419525576112045No Hit
GCATCTTGGGATTCCATTCTCAAAATGACTTTGGAGTGAAAGTAATTATTTT1880.11359104805868064No Hit
TGAGTAGGGGACTTCCTGGTGGGATCCGAGTGAGGCGACGGGGTAGGGGTTG1870.11298684035624086No Hit
ATTGCAGGGCTTGTGGCTATTTTTAAGATTTTCTTTGTGTTTTGTTTTTCTC1860.11238263265380107No Hit
CTAAAACGGGCACCTTATTTTTGGTCTTTGTTTATTCATTTAGACCCTGCAA1860.11238263265380107No Hit
CTCAGAACGGGAAGATGACATTGTTAGTGTTCACGTCCCATGTTCAGTGTCC1860.11238263265380107No Hit
TACAGTGGGGTGCAAGGGGGACTGGGCCCAAACCACAAAACCTTTTTTCTTC1850.11177842495136128No Hit
CTATATGGGAAATTTTAATTAAAAAATCAAGGAGTCTTCTAATGTAAATTTT1850.11177842495136128No Hit
CCGCATGGGAGACTAAGAAAGGATGAAGAGGTAAGAGTAGAAAAATGACCAG1840.1111742172489215No Hit
GATTATGGGTGTAGCTAAAAGTTCTCTTTTTCTTCTAGTTCTGTGTCCTACA1840.1111742172489215No Hit
AATTGCGGGATTTCCATTTTTAGGAGATCAAAGTTTGCAGGATTTGCAAAAG1820.1099658018440419No Hit
TTCAGTGGGGACTGCTGGTTTACATTTCTGCTCTTTCTAATTCAGTTGTCTA1810.10936159414160211No Hit
TTAGACGGGATTAAGTAGCACTCTTGAGGAGTGTTCTCTTTGACTTCTAAGA1810.10936159414160211No Hit
CAAGCTGGGGATCTTGCTAGGGGTACTGCAAAAACAAGACAAAACTAAAAAA1810.10936159414160211No Hit
TTGTTAGGGAGTGACCATCTCTGTAGTTCAGTTAACACTTTACTCCCAACTT1800.10875738643916233No Hit
GATAATGGGAGCAAACAACAAGTTGAATGGCAACTTGACATTTTTGCATCAC1800.10875738643916233No Hit
GTAAATGGGGGGAGAAAATATTTGCTAACTACTTATTTGATAGGGAATTAAA1790.10815317873672253No Hit
AATCCGGGGATCTTTCGGGGGGAAGCCTTTGCAAGAAAATTGCATACAAGGC1790.10815317873672253No Hit
GTACAAGGGGTATCTTTTAATATAAAAGAAGCTTTTAACTTTTCCAGCCTAT1780.10754897103428275No Hit
TACCATGGGGATAATATTAAAGTTCCACAGAAAAAAATTAGGAAATGCTGGA1780.10754897103428275No Hit
AAATCAGGGGGTCCGCCGGATCTCCAGCGCTCAGTCCGCGCCGCAGGTGGTG1750.10573634792696338No Hit
GCTTCGGGGTCACTGACAGTACAGTGTAATCTGGACTAACATCATTTATGCA1740.10513214022452358No Hit
TGCTTAGGGACTCATTTAAATATTTTTAAGAAAATAAAAAGCTTAAGAAATT1730.1045279325220838No Hit
GTATATGGGCATCAGAATGATGCCCCTGATAACCTTGCCCATAACCATGCAT1730.1045279325220838No Hit
TCAACTGGGATTCCAGGAGGAAAGAGGGTTTTCATTCCCTTCTGAATGTTAG1730.1045279325220838No Hit
ATATTTGGGAGTGATTGCATGTTGAGAAATTCTCTGTCCCCTAACATCTTTA1730.1045279325220838No Hit
ACCTTAGGGGTTCTCTCCCCTTCTCCACAAAAAAGTATTCTCTGCACTTGAA1720.10392372481964401No Hit
CTCCAGGGGATGGTCATGAACCTTTGCAGATAAGGAAACTGTGTTTCACAAG1720.10392372481964401No Hit
TTCGTCGGGGTCTGCATTCACTGAGATGACTTTATGTATTTAAAAAAATCTG1710.1033195171172042No Hit
GAAGGGGGAGTTAACATTTGTTTGGAAACAAAGCATCTGATGAATGTGTCTG1700.10271530941476442No Hit
CAATATGGGGTATTAACTTGTAATAATTCCACAATTCCTCTAAAGGTTATAT1700.10271530941476442No Hit
CAAATCGGGGTGAAGTTATCTGTACTGAGGAATAGGTGATCAGTAATATCAG1700.10271530941476442No Hit
CAAATTGGGGGCGCCCGCCACTACGCCCGGCTAATTTTTTGTATTTTTAGTA1690.10211110171232463No Hit
TGGTCTGGGGGATTGACCAGAGGAATAACTTAGCTTGCACATGAATGCAGTC1680.10150689400988484No Hit
ACTGAAGGGCTGGTGGTGAACGGGAACCTGGTCCACACTGCAGTCGTGCCGC1680.10150689400988484No Hit
GCTCCTGGGGGACACTGACAAATTCATGTTTACTATAAAATCTCCTTACATG1680.10150689400988484No Hit
TACTGTGGGTTCAGTCAGTTGGGAGCTTAGAATTTAATTTTCATTTATCAAT1680.10150689400988484No Hit
AGTGCCGGGGATTCTCGAGTTAGAGAGGTGATCAATAGAAATCTGTTGGATC1670.10090268630744506No Hit
GTAATTGGGGATGCCTCACTTGCTTTTTGAATGGTAAAGCAGTAAGATTTAT1670.10090268630744506No Hit
ACTTAGGGGGGCGCTGGGGTAACTTTGATAAGAATAGAAACGTGACACGATC1660.10029847860500525No Hit
AATACTGGGACAGAGAGGACATTGTGGGCTAATCATATGCTTATTTACACCT1660.10029847860500525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)