FastQCFastQC Report
Sat 31 Dec 2022
SRR5843990.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843990.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164657
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT5600.34010093709954636RNA PCR Primer, Index 17 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC5440.33038376746813075No Hit
TTCCGAGGGGAGTCTTTGGAGGCACTCAAGCAGCAGCAAATGAAGCTCGTGA2140.12996714382018376No Hit
GCTTTTGGGATCGATTGGAACCCTGATTGGCGGCGTCTACCCCGGGAGCTAA2120.12875249761625682No Hit
CTTAGAGGGTACAACCAGAGATACAGGTTTTCCATCAAGAGGGGGCCTATAG2100.12753785141232987No Hit
TAAAATGGGGTGCTCAGGGCAGGCCTCGCCTAGAAGATGATGTTTGAACAAA2080.1263232052084029No Hit
TGAAAAGGGAACACTCCAAGATGGGTTCATCTTTTCTTCCTTTCCCATTCAC2060.12510855900447596No Hit
CCATCCGGGATGAGCCATGAACCAACCAATGATAAGTCTACTTTTCTTCCAC2040.12389391280054902No Hit
CAAGCTGGGGATCTTGCTAGGGGTACTGCAAAAACAAGACAAAACTAAAAAA2040.12389391280054902No Hit
TACATGGGGACTAAGATCATCCTTGGCCCCCACCTTTATCCTTGTTAATGCT2030.12328658969858555No Hit
AAGCACGGGCGATCTGTCAGTGGGCCCATTTGGTCCCGACAAGATGACATAG2020.12267926659662208No Hit
AGCTAGGGGTGATACAGCTGAATGAAGAAAGAATGCTGGAGAAAATGTAAGG2020.12267926659662208No Hit
TTAGCTAGGGGGGCGAGCGCCCGGGCGGCGGCGGGGGCGCCAGGAGAGTCCG1970.11964265108680469No Hit
TTCAGTGGGGACTGCTGGTTTACATTTCTGCTCTTTCTAATTCAGTTGTCTA1940.11782068178091426No Hit
TATACAGGGAAGTGAGTGAAGAATTTTTCATTACAGGAGGCTTTAATGCATA1940.11782068178091426No Hit
CTCCAGGGGATGGTCATGAACCTTTGCAGATAAGGAAACTGTGTTTCACAAG1940.11782068178091426No Hit
CTTACGGCGGGAGCGCACCGGGCCTGCCGAGGAGCGCGCGCCCGGCCGGGCT1930.11721335867895079No Hit
GTAAATGGGGGGAGAAAATATTTGCTAACTACTTATTTGATAGGGAATTAAA1920.11660603557698732No Hit
CTATATGGGAAATTTTAATTAAAAAATCAAGGAGTCTTCTAATGTAAATTTT1920.11660603557698732No Hit
ACAACCGGGAGAGTCTTCTCATTAACTTCCATGACAATTATTTCTCTGCATG1910.11599871247502384No Hit
ACATCAGGGATCTGGATGGCGGTGGAAAAACTTGAGGGTTTGGGGTTCAAAG1900.11539138937306036No Hit
AAATTAGGGATAGCCTTGAAAGTTCTTGGTTTGCCTTTACATTTTTATTTCT1890.11478406627109689No Hit
ATATTTGGGAGTGATTGCATGTTGAGAAATTCTCTGTCCCCTAACATCTTTA1890.11478406627109689No Hit
TGTAAAGGGATCACAGAAATAATGTTGCAGTTATGGTTATAGCACAAGGCAA1880.11417674316913341No Hit
GCAATGGGCTCTTTGTGGCTCAGAAAATGTACAGGTTTTCCAGGGCAGCCTT1880.11417674316913341No Hit
AGTCGCGGGTACTAATCGAATTATTTCTCATTTTAGTCAAGATGATTTTACT1860.11296209696520645No Hit
TTACTCGGCTCCTCAGCAGGTCCCGCTTCCACGTGGGAGATTGGATGGGCAC1850.11235477386324298No Hit
TACACCGGGTCTTTTAGAGGGACCAAGATTTCTTAAGACAATCTACTTTACT1830.11114012765931604No Hit
AATCGTGGGTCTTTTTGTATGTAGTTTTATGAAATTTCATACATGTATAGAT1820.11053280455735257No Hit
TACCGCGGGAGAATTGATGCTCTATTAATAAGAGTGGTGGTGGTGTTTTTTA1820.11053280455735257No Hit
AATACTGGGACAGAGAGGACATTGTGGGCTAATCATATGCTTATTTACACCT1800.10931815835342559No Hit
CTGTTCGGGAACCTGACATCATCTTTCCACCCGGCTTACCTCCACCAGATCT1790.10871083525146212No Hit
TACAGAGGGTGGGTTTCTTGGAGTACTGTTAAATCCTTTTTACAAATATATA1790.10871083525146212No Hit
TGAGTAGGGGACTTCCTGGTGGGATCCGAGTGAGGCGACGGGGTAGGGGTTG1780.10810351214949865No Hit
TGCACAGGGAATGCCCTCCCCTGTTAAAGTAAATTCAAAAAATTGAAGGCAG1760.1068888659455717No Hit
TGCTTAGGGACTCATTTAAATATTTTTAAGAAAATAAAAAGCTTAAGAAATT1760.1068888659455717No Hit
ACATCGGGGGTTAATAAAGATTTGGGAGTATTAGTGGTCTTAAAATGGACCA1760.1068888659455717No Hit
AGCCCAGGGGTTTGACCTGTGATCAAAATTGAACCATTGTACAGTTTGGCTT1760.1068888659455717No Hit
TTGTTAGGGAGTGACCATCTCTGTAGTTCAGTTAACACTTTACTCCCAACTT1750.10628154284360823No Hit
GTAATGGGATATTACCTTAATTTTTATTATCTTAGTTTCCACATAGTAATTT1750.10628154284360823No Hit
GCATCCGGGAGTGTCTGAGCAAGGAAGAGGCTAAGGCTTCCTTTCAACATTC1750.10628154284360823No Hit
GGGCAGGGGGCAAGCTTGCTTTTATTTTTGTCATATAAGTAATTTAAATCTT1740.10567421974164476No Hit
TACTGTGGGTTCAGTCAGTTGGGAGCTTAGAATTTAATTTTCATTTATCAAT1740.10567421974164476No Hit
AATCCGGGGATCTTTCGGGGGGAAGCCTTTGCAAGAAAATTGCATACAAGGC1740.10567421974164476No Hit
CTACTAAAAATACAGAAAATTGGCTGGGCGTGGTGGTACACGCCTGTAATCC1740.10567421974164476No Hit
CGACATGGGCTCACATTCTTTATTGATTGATTTCTTTTTATTTACGATCTTA1730.10506689663968127No Hit
TTTGCCGGGATCAAAGGAAGAATAAATACTCTGCCCAAATTTTCAGTGTTAT1730.10506689663968127No Hit
GCCAATGGGTAATAAAGAAACAAATCTTAATTAAAATAAAGATACCGAGCAC1730.10506689663968127No Hit
TGTAGCGGGGGCTGAGGTGGTAGGATTGCTTGAGCCCAGTAGTTAGAGATCA1730.10506689663968127No Hit
ACGCCTGGGGGTGAAGCGGGGGTGGGATCTGAACATGGCGGCGGTGGTAGCT1720.10445957353771779No Hit
AGTGCCGGGGATTCTCGAGTTAGAGAGGTGATCAATAGAAATCTGTTGGATC1710.10385225043575431No Hit
GGGACCGGGAGTGTTAGTTAGTGCCAAATGTGGACCACTGGCAGAAGTCTTC1710.10385225043575431No Hit
ATTGCAGGGCTTGTGGCTATTTTTAAGATTTTCTTTGTGTTTTGTTTTTCTC1710.10385225043575431No Hit
GATTATGGGTGTAGCTAAAAGTTCTCTTTTTCTTCTAGTTCTGTGTCCTACA1710.10385225043575431No Hit
TCATCCGGGAGAAGAGCATGGCTGTGTTCCAGTAAAACTTTATTTACAGGCC1710.10385225043575431No Hit
CTTTCAGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT1700.10324492733379084No Hit
GCTCCTGGGGGACACTGACAAATTCATGTTTACTATAAAATCTCCTTACATG1690.10263760423182737No Hit
AATACAGGGAATCTCAGAAGGTAGCATAAACAAATAAAAATTAACTGTATGG1690.10263760423182737No Hit
ATGCCAGGGATAAACATGTGAGTTGAATAACTTTTAAAAGCAGAGTCTGTCT1680.1020302811298639No Hit
GTATATGGGCATCAGAATGATGCCCCTGATAACCTTGCCCATAACCATGCAT1670.10142295802790043No Hit
TCCAAAGGGACATCCTACTGATCTTCAAAAAGTTGCAGAAATGATTCGAGAA1660.10081563492593695No Hit
GACGCTGGCCTGAATGGTATTTCCCAGGTTTTCTCCTAGGGTTTTTATGGTT1650.10020831182397348No Hit
GAAGGGGGAGTTAACATTTGTTTGGAAACAAAGCATCTGATGAATGTGTCTG1650.10020831182397348No Hit
TACGTGGGGGAGAAACGAGCTTGGAAGGAGAGCGAGGGTGGGGAGCAGAAGC1650.10020831182397348No Hit
AATTGCGGGATTTCCATTTTTAGGAGATCAAAGTTTGCAGGATTTGCAAAAG1650.10020831182397348No Hit
GGGGTGACAGTATGGCATGTACTTTTTTTAAAGAGAATAAAACTGAAAAAGA1650.10020831182397348No Hit
TAAATCGGGGGAGTGATGGCGCCGTCTATGTTCGACTAAACTAAAGTGTCAC1650.10020831182397348No Hit
GCTTCGGGGTCACTGACAGTACAGTGTAATCTGGACTAACATCATTTATGCA1650.10020831182397348No Hit
GTGACTGGGAACTGTTTTCTTGCAGCAGCTTTATGATGAGAATCACTGTTTC1650.10020831182397348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)