FastQCFastQC Report
Sat 31 Dec 2022
SRR5843980.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843980.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73878
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC3780.511654349061967No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT3700.5008256855897562RNA PCR Primer, Index 17 (95% over 22bp)
TAACAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1180.1597227862151114No Hit
GGCCCTGCGTCAGAGGCTGAAGTGTCGCAGCTTCAAGTGGTACCTGGAGAAC1140.15430845447900593No Hit
AACAAGGGGATAGAAAGCAGAAGTAGGATGATTAGAATAGGTATCTAGCAAT1110.15024770567692683No Hit
CTTCATGGGCAGCTTTAACCATATTGGCTTTAAGATGAGCGAAAACAGGGAA1080.14618695687484773No Hit
GCTTGGGGGTGTGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTG1010.13671187633666315No Hit
CGCTGCGGGAATGAAAAACCAGGCATCAGAACAAAAAGAAAAAAGAAAAAAA990.13400471046861043No Hit
AATGCAGGGAATTTTGATTAATATCTTTTTTCAGACTTAGTCAACATGTGAT990.13400471046861043No Hit
TGTACCGGGATCTCTGGTTGAGTGTTTTTGTAATCTTGCTTTCTTTTCACTG990.13400471046861043No Hit
CTTTTTGGGTCCCATCCCCATGCCCCGTCCGCGGGGCTGTGGCTGCTGCTGT970.13129754460055768No Hit
GAGTCTGGGCTTCCTGTTCTGGTTTTGTTTTGTTTTTTGTTTCCTTTGGCTG960.1299439616665313No Hit
GCTATTAGGGCTGAACGAATGAGCACACTTTAACCAGTTATTTACAGTTACC950.12859037873250495No Hit
GTAAAAGGGGCAAGCAGTAATTCATAGGTTTCACCTGAGCAGTAGGAGGGAT930.1258832128644522No Hit
ATAGTGCGGGGTACCTGGTTCTTAAATACTCAGTCACTGAGATTTACTTAGG930.1258832128644522No Hit
TTGCTAGGGGGTCTCGGAAACCGGTAGCGCTTGCAGCATGGCTGACCAACTG920.12452962993042584No Hit
AGGACTGGAGCACAGGAATGTTTCTGATTACACACCTCTAAGTCTGGCTGCT910.12317604699639947No Hit
GAATTGGGGTCAGTCGTTTCTGCATGAAGATAGGAATTACTGATGAGAACAT900.12182246406237311No Hit
CATATAGGGCAAAGCCAGTTTTTGTTGCTTTTCTAAAGCAACAAATAATTCT900.12182246406237311No Hit
TTAGCCGGGGAAGATGGAAGGAAAAGGTAGCAAAGGAAAGACAGCAGAAATA880.11911529819432036No Hit
GATCCCGGGATTCAAGACAGAAAGAACAGCTAGAGCCAAAATACAATTCAAA880.11911529819432036No Hit
ATAACAGGGATGGCCCCATTTCTCCATTTTGTGGACCAAGCCATCCTGAGGG880.11911529819432036No Hit
AGGCCTGGGAATTGGTTTACCATGTAATGACACTTCCATTTTAAAGATTTTA860.11640813232626764No Hit
TAGAACGGGCTACTGGGTACATAACGAAATGAAGGCAGAAATAAAGATGTTC830.11234738352418852No Hit
TTAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC830.11234738352418852No Hit
GTGGCAGGGATTGAAACAGCAACTGGTTAAGGTTTATGAAAAATATGGTTAT830.11234738352418852No Hit
TAATTAGGGAAGTAAACAAATCTAGGTTTGCCAGTGTATTATTTCAACTAGA830.11234738352418852No Hit
TTATAAGGGGGTTTGAGGGTAATTTTTCTTGATAGGTTTAAAAATAAACATG830.11234738352418852No Hit
GCTGCTCGGGAGGCTGAGGCAGGAGGATTGTTTGAGCACAGGAGATTGAACT820.11099380059016216No Hit
AACCATGGGAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGTTGG820.11099380059016216No Hit
AATCAAGGGTGGTGCAGGTCACTACCCACAGAAGAACTGACCAAGAAGAGAG810.1096402176561358No Hit
TATGAGTGGGGCATGATGTGTTTGTGTGTCACAAATCCAGAAAGTGAACTGC810.1096402176561358No Hit
ACACACGGGTAGCACTCCATTTTCAATAATTGATAGAAAAACAACCAGATAG800.10828663472210942No Hit
TTGCAAGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT800.10828663472210942No Hit
GTCACCGGGATTTTAAGACTTTTAAAAAACAGAATGAAGATTATTATTAAAT800.10828663472210942No Hit
GTTCATGGGAAGGGAGGAAAAAAAACTGAGAAGCACTGGTGAAGGTTGCAGT790.10693305178808304No Hit
GTCAACGGGAGGCATTGAGGCAGCCAGCTCAGGGGCTTCTGCTGAGGGGGCA790.10693305178808304No Hit
ATTGAAGGGATGTTTACCATAAGCAGGTACAAGCTTCATGAACCGTTCTTAA790.10693305178808304No Hit
AGTTGAGGGTGCCTGCTTAAACAAAAATGTCAACATTTTCTGTAATATTTAA790.10693305178808304No Hit
AGCATGGGGAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATA790.10693305178808304No Hit
TAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC790.10693305178808304No Hit
AGCTCCGGGATCCCGAGGGAAGTCCTCCAGTCTAAGTCACAGTGCCCTGACA780.10557946885405668No Hit
TCACAAGGGGAAATAAGATCGTTTATAGTTGGATGACAGAGCCAACTAGACG780.10557946885405668No Hit
GAAGATGGGATAGCATCAGGAAACAACAGATGTTGGCAAGGATGTGGAGAAA780.10557946885405668No Hit
AATGCTGGGAGAGATATGTTTTCTGGAGTCATAAAGGAATTCAATTCCTAGG780.10557946885405668No Hit
AAGCAAGGGATCTGAGAAGACATCGGACTGGCAAACGGTAAGTCGTGTTACC780.10557946885405668No Hit
CAATTAGGGGTCCAAAGAAATTTTTGCCAATCACAACTTGAACAGATTTTCT780.10557946885405668No Hit
ATACTAGGGGGTACTCGTGCGAGGAGGAGCTCAGCGGGCTTGGTGAAAAATG780.10557946885405668No Hit
CTAACCGGGATCGAGGATACTGGCATGGCTGCAATCCACTGACCTTATTTGA780.10557946885405668No Hit
TGGCATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA780.10557946885405668No Hit
GGATGCGGGGCGCGTGGATCCGAGCGACCATGGTGGCCCGGGTGTGGTCGCT770.10422588592003032No Hit
AAATGTGGGAGTATGACTGCCAATATATAGTTCTAGTGGGATACCCACAATG770.10422588592003032No Hit
ACTAGTGGGAAGCCACAGACCGGAAGTATTATTTACAATAAGTATATCTAAC760.10287230298600396No Hit
GATTGAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG760.10287230298600396No Hit
ACAGCTGGCTATGCAATGAATAGATATGCATATTATGTGTGCTACAAATGCA760.10287230298600396No Hit
GAACAAGGGCTAGAGACGCTGAGCATGATTACATTAGCAACTTGACTGTTTA760.10287230298600396No Hit
TACAACGGGATTGAGAAACATGTATGCCAGGTGAGGCTCTGTACATATTTAA760.10287230298600396No Hit
GGCACCGGAATCTCTCCAGGAGAAGCTCGGCGTTACCCCCGGCAGCTGGTGG760.10287230298600396No Hit
TATGCCGGGTCAAGGAACCATGGGCTTTGTTGGGAGGTGGCTGTACTGAAAC750.10151872005197758No Hit
GCGTTTGGGCTGGCCCAGTATCTTCCTGACAGCCCCTTAGCACAGGTTACTC750.10151872005197758No Hit
TCAACAGGGGGGCTGTAAGCTGCTGCTTATGTTGAAAAGTGGTTCTTCAGAT750.10151872005197758No Hit
GCCGAGGGTGTGCTTGCTGCTGCTGTGAGTGAACCATTGCTTAAGATAAAAA750.10151872005197758No Hit
GGTAGCGGGATGACCCCAATACTCAAAACTAACCCCCTAATAAAATTAATTA750.10151872005197758No Hit
CTAACTGGGTGCTTACCAATTGATGGAAAATGGCAGCTTAGAGAATCTGTGG750.10151872005197758No Hit
GCTTTTGGGGGAGTGGACAGCCAGCGCGGGTCCCGGCCCCGGTGTGGAGCCC750.10151872005197758No Hit
GCTTCTGGGGGGATGGCGAATTCTGTAGATGTCTATTAGGTCTGCTTGGTCC740.10016513711795122No Hit
TGATGCGGGGATGCTGCAGCTTAAATGATATTATACTTTGAAAACCTCTGCT740.10016513711795122No Hit
TTCCAGGGGAGTTTTTCTTAATTATAACTTCTTAGGGTTAGAAAACAATAAT740.10016513711795122No Hit
GCGCGAGGGGCTCAATAAGAGACATCAGGAGGTTTGGCTTCTTTTCAGAGGA740.10016513711795122No Hit
CGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG740.10016513711795122No Hit
GCAGCGGGCTTACCATACAGTACAGTGGTTTCAAAATTTTTATCTGTATTCT740.10016513711795122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)