FastQCFastQC Report
Sat 31 Dec 2022
SRR5843979.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843979.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73072
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT4030.5515108386249179RNA PCR Primer, Index 17 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC3450.4721370702868404No Hit
TAACAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1190.1628530764177797No Hit
AATGCAGGGAATTTTGATTAATATCTTTTTTCAGACTTAGTCAACATGTGAT1090.1491679439456974No Hit
ATAACAGGGATGGCCCCATTTCTCCATTTTGTGGACCAAGCCATCCTGAGGG1070.14643091745128095No Hit
TAGAACGGGCTACTGGGTACATAACGAAATGAAGGCAGAAATAAAGATGTTC1060.1450624042040727No Hit
GGCCCTGCGTCAGAGGCTGAAGTGTCGCAGCTTCAAGTGGTACCTGGAGAAC1030.140956864462448No Hit
GAGTCTGGGCTTCCTGTTCTGGTTTTGTTTTGTTTTTTGTTTCCTTTGGCTG980.13411429822640683No Hit
GCGTTTGGGCTGGCCCAGTATCTTCCTGACAGCCCCTTAGCACAGGTTACTC940.1286402452375739No Hit
TTAGCCGGGGAAGATGGAAGGAAAAGGTAGCAAAGGAAAGACAGCAGAAATA920.12590321874315744No Hit
TAATGAGGGATGTGAATAAAATACGTATGTTAAATCAAAGATTTAGTAACTC910.1245347054959492No Hit
CTTCATGGGCAGCTTTAACCATATTGGCTTTAAGATGAGCGAAAACAGGGAA900.12316619224874095No Hit
TAACATGGGAGTGACTAATTTTCCCTTACAGTTCCTGCTTGGTCCCACCCAC900.12316619224874095No Hit
AACAAGGGGATAGAAAGCAGAAGTAGGATGATTAGAATAGGTATCTAGCAAT890.12179767900153274No Hit
TCATCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.1204291657543245No Hit
CTAACTGGGTGCTTACCAATTGATGGAAAATGGCAGCTTAGAGAATCTGTGG880.1204291657543245No Hit
AGCATGGGGAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATA880.1204291657543245No Hit
GTTTCAGGGGAATGTGAAGAGCTGGGATCAGATGAAGGAGAAGTCCATACTC870.11906065250711627No Hit
GATTGAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG860.11769213925990804No Hit
AGGACTGGAGCACAGGAATGTTTCTGATTACACACCTCTAAGTCTGGCTGCT860.11769213925990804No Hit
TTGCTAGGGGGTCTCGGAAACCGGTAGCGCTTGCAGCATGGCTGACCAACTG860.11769213925990804No Hit
TAGCGTGGGGATAGTACTCTATTCGTATTAGGAAAGAGAGACTAGATACCAA860.11769213925990804No Hit
TTTCGCGGGGGAGACCCAGACGCAGAGGCGGGGCTGGCCCCTGGGGAGCTGC860.11769213925990804No Hit
TGATGCGGGGATGCTGCAGCTTAAATGATATTATACTTTGAAAACCTCTGCT850.11632362601269981No Hit
ATGTAAGGGGGTCCAAGGAAAAACAAAAGGTAAAGGAAATAAAGGGAATATA850.11632362601269981No Hit
GACAATGGGATGACACTGCCTGGGATGAGCTGAAGCTCACGTGTCAGACGGC840.11495511276549157No Hit
GAAGATGGGATAGCATCAGGAAACAACAGATGTTGGCAAGGATGTGGAGAAA840.11495511276549157No Hit
GTATGTGGGCTTTGCAAACACATTATGCTTTTGCACACACACACCTGTACAC840.11495511276549157No Hit
CCGTTTGGGATCAAAAGGATTGGATAATAAAAATAAGTTTCTACTGGGTATA830.11358659951828333No Hit
ATTAGAGGGAACCAGTCCAAATAGACATCAACCAATTGTGAGTTCTGTCCTA830.11358659951828333No Hit
AGTCTCGGGTGCTCGCGAGGGTGACCTCCTGACCAAGGAGCGGCTGTGCTGT830.11358659951828333No Hit
TATGAGTGGGGCATGATGTGTTTGTGTGTCACAAATCCAGAAAGTGAACTGC830.11358659951828333No Hit
GCTTGGGGGTGTGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTG830.11358659951828333No Hit
AGCTTTGGGGTCGCCCATAGTTGTAGTTTTGGGTTCTGGCAGGTGGAACACC830.11358659951828333No Hit
TAATTAGGGATTCTCCATAAAGATGGCATCATCAACAGGAAGGTCCTAGGCA820.11221808627107512No Hit
AGTATGGGCATTGGTGCTTTTTGCTTTGTCCTGTGGTAATAAGAAGGATCAT820.11221808627107512No Hit
AGACCTGGGATTGCCTTTTTATTGTAAGATATAGAAAGGTATTTATTTGTTC820.11221808627107512No Hit
GCTCGCGGGAGGCTGCAGTGAGCTATGATCATGCCACTGCACTCCAGCCTGT820.11221808627107512No Hit
TTAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC820.11221808627107512No Hit
TGTACCGGGATCTCTGGTTGAGTGTTTTTGTAATCTTGCTTTCTTTTCACTG820.11221808627107512No Hit
TTGTGGGGGTAGCAGGACTGATCGTTTTTCTAAGTAGACCTGAGCTTTGTTT820.11221808627107512No Hit
CTAACCGGGATCGAGGATACTGGCATGGCTGCAATCCACTGACCTTATTTGA820.11221808627107512No Hit
TGGTATGGGTAATCAACAACTAAATCTTTTGCCAAATGCATGCTTGCCTTTT810.11084957302386687No Hit
CCTATTGGGTAGCCAAGCAACCATAATAATTGGCGGGAAAGAGCAGGGCCAG800.10948105977665863No Hit
GCCGTTGGGCATACTGAATAAAAAGTGGCTCATAATAAATGCCAAGGAATCA800.10948105977665863No Hit
AGTTTCGGGGAAACATTAAAAAATATTTTTTGAGTTTATGCTTTGAACGATA800.10948105977665863No Hit
GCTACCGGGACGCTCTTCCGATCTATAACTGGGAGGCATTGAGGCAGCCAGC790.10811254652945042No Hit
GCTATTAGGGCTGAACGAATGAGCACACTTTAACCAGTTATTTACAGTTACC790.10811254652945042No Hit
TTGCAAGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT790.10811254652945042No Hit
CAATTAGGGGTCCAAAGAAATTTTTGCCAATCACAACTTGAACAGATTTTCT790.10811254652945042No Hit
GCAATGGGGGGATTTTCCAACAGAAACCATTAAATTTGGTCCTGAACGCATG790.10811254652945042No Hit
GGCAATGGGATATTTCTGCATGCCCTACTCTATCATCAAAGTTTTGGCAACA790.10811254652945042No Hit
TTATAAGGGGGTTTGAGGGTAATTTTTCTTGATAGGTTTAAAAATAAACATG790.10811254652945042No Hit
TGTACGCGGGTACGCCCTTGAAAGCACTGGGATTACAGGTGTGAGCCACCAT790.10811254652945042No Hit
GTCACCGGGATTTTAAGACTTTTAAAAAACAGAATGAAGATTATTATTAAAT780.10674403328224218No Hit
GCGCGAGGGGCTCAATAAGAGACATCAGGAGGTTTGGCTTCTTTTCAGAGGA780.10674403328224218No Hit
AGCAAAGGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGG780.10674403328224218No Hit
AACCATGGGAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGTTGG780.10674403328224218No Hit
CGTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA780.10674403328224218No Hit
TAATTAGGGAAGTAAACAAATCTAGGTTTGCCAGTGTATTATTTCAACTAGA780.10674403328224218No Hit
AGTTCAGGGACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGCTC770.10537552003503393No Hit
ACGAGGGAAGACCTGCTGAGCCTCAGGGTCTGGGATAGAGCCTGGGAATCTG770.10537552003503393No Hit
CGCTCTGGGATAGAAACTAGGAAGATTGTCAGAGAGTTTTCTGGACACCAAG770.10537552003503393No Hit
AAACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA770.10537552003503393No Hit
GCCTCGGGCATATGAAGTCACCCTAGCCATCATTCTACTATCAACATTACTA770.10537552003503393No Hit
GCTAAAGGGCTGGTGGCAATGTACCTTGCTAAACATTATAGTACTATTTGTA760.10400700678782569No Hit
TCAGATGGGGACTAGAAGGCGAGGCGCCGCGGGACCATGGCGGCGGCGGCGG760.10400700678782569No Hit
ATGTGGGACCTCTCTATACTAATCTCCCTACAAATCTCCTTAATTATAACAT760.10400700678782569No Hit
ACTGCTGGATTTGCTTTGCCAGTATTTTACTGAGGATTTTTTCATCAGTGTT750.10263849354061748No Hit
AAGGCAGGGAGAAACTGCAGTAGGGGCTGCCATCAAAAGTTTTGAGAATTCT750.10263849354061748No Hit
TTATCAGGGGATAATAGGTATCCTAACAAGTTTGATATGACAGCTCATTGTA750.10263849354061748No Hit
GTAAAAGGGGCAAGCAGTAATTCATAGGTTTCACCTGAGCAGTAGGAGGGAT750.10263849354061748No Hit
ACACTTGGGAAGTAGGCATGGTGGGAATATTTACACTAGGGAAATCAGCAAA750.10263849354061748No Hit
ATTCGCGGGTCCCCTGCAATTAGAAAAAAATGTTACTATTCTAACCAATGCT750.10263849354061748No Hit
TCTGTGGGGGGCGCACGCGCAGTGACGCGCCGGCCATGCCGGCGGCTGTTGT750.10263849354061748No Hit
GTGGCAGGGATTGAAACAGCAACTGGTTAAGGTTTATGAAAAATATGGTTAT750.10263849354061748No Hit
AGATATGGGTGTTTATTCATTAATCATTAAGCCCGTGAGATAGATGAGTATT750.10263849354061748No Hit
GTGCTTGGGAGAATAAAACCTTATTATTTTTTACACTGCACATGCTGTTCTC750.10263849354061748No Hit
TTGAAAGGGGTAACATTGCTCTGACAGGTATCACTAATTAAATATTGTTCAA750.10263849354061748No Hit
AGCTCCGGGATCCCGAGGGAAGTCCTCCAGTCTAAGTCACAGTGCCCTGACA740.10126998029340924No Hit
GCGCACGGGATTTTTTGTAGAGTTCGTATAAGCTACCCTAAAATAAATATGG740.10126998029340924No Hit
GGCGCTGGGGGTAGGCAATAGAATATATATGTCTGACACTCAAGTAGGGGTA740.10126998029340924No Hit
ATTGAAGGGATGTTTACCATAAGCAGGTACAAGCTTCATGAACCGTTCTTAA740.10126998029340924No Hit
CGCTGCGGGAATGAAAAACCAGGCATCAGAACAAAAAGAAAAAAGAAAAAAA740.10126998029340924No Hit
AATTTCGGGGGCCTCCCAAAGTGCTGAGATTACAGGCGCGAGCCACCGTGCC740.10126998029340924No Hit
GTGCGAGGGATACATGGAATTGATATTGCTTATTCTGACTAAGTGAAAAGTG740.10126998029340924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)