FastQCFastQC Report
Sat 31 Dec 2022
SRR5843978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73670
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT3720.5049545269444822RNA PCR Primer, Index 17 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC3200.4343694855436405No Hit
TAACAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1250.1696755802904846No Hit
GAGTCTGGGCTTCCTGTTCTGGTTTTGTTTTGTTTTTTGTTTCCTTTGGCTG1100.14931451065562645No Hit
CTTCATGGGCAGCTTTAACCATATTGGCTTTAAGATGAGCGAAAACAGGGAA1060.14388489208633093No Hit
GTAAAAGGGGCAAGCAGTAATTCATAGGTTTCACCTGAGCAGTAGGAGGGAT1010.13709786887471154No Hit
AACAAGGGGATAGAAAGCAGAAGTAGGATGATTAGAATAGGTATCTAGCAAT990.1343830595900638No Hit
TTGCTAGGGGGTCTCGGAAACCGGTAGCGCTTGCAGCATGGCTGACCAACTG980.13302565494773994No Hit
AATGCAGGGAATTTTGATTAATATCTTTTTTCAGACTTAGTCAACATGTGAT970.13166825030541604No Hit
CAACAGGGGATTCATTGTTCAATGAATATTTTTGTTCCTATAGTGGGTTAAT940.1275960363784444No Hit
CTTTTTGGGTCCCATCCCCATGCCCCGTCCGCGGGGCTGTGGCTGCTGCTGT940.1275960363784444No Hit
TGTACCGGGATCTCTGGTTGAGTGTTTTTGTAATCTTGCTTTCTTTTCACTG940.1275960363784444No Hit
CGTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.1275960363784444No Hit
ACTGCTGGATTTGCTTTGCCAGTATTTTACTGAGGATTTTTTCATCAGTGTT910.12352382245147278No Hit
AATGCTGGGAGAGATATGTTTTCTGGAGTCATAAAGGAATTCAATTCCTAGG910.12352382245147278No Hit
CGAAAGGGATGAGTGGATATGACCAAGTTCTGCAGGAAAACTCCAGTGACTA910.12352382245147278No Hit
ATAACAGGGATGGCCCCATTTCTCCATTTTGTGGACCAAGCCATCCTGAGGG900.12216641780914891No Hit
GTCACCGGGATTTTAAGACTTTTAAAAAACAGAATGAAGATTATTATTAAAT890.12080901316682503No Hit
CGTTTTGGGCTATGGAGGGGCAGGGAAAAGGTGGCACTTGTGAGTGTGTGTG880.11945160852450117No Hit
GAACAAGGGCTAGAGACGCTGAGCATGATTACATTAGCAACTTGACTGTTTA880.11945160852450117No Hit
GCTAAAGGGCTGGTGGCAATGTACCTTGCTAAACATTATAGTACTATTTGTA870.11809420388217727No Hit
TACAACGGGATTGAGAAACATGTATGCCAGGTGAGGCTCTGTACATATTTAA870.11809420388217727No Hit
GGCACCGGAATCTCTCCAGGAGAAGCTCGGCGTTACCCCCGGCAGCTGGTGG870.11809420388217727No Hit
CAATTAGGGGTCCAAAGAAATTTTTGCCAATCACAACTTGAACAGATTTTCT870.11809420388217727No Hit
TAGAACGGGCTACTGGGTACATAACGAAATGAAGGCAGAAATAAAGATGTTC860.11673679923985342No Hit
CCTATTGGGTAGCCAAGCAACCATAATAATTGGCGGGAAAGAGCAGGGCCAG860.11673679923985342No Hit
GCTATTAGGGCTGAACGAATGAGCACACTTTAACCAGTTATTTACAGTTACC860.11673679923985342No Hit
GGCCCTGCGTCAGAGGCTGAAGTGTCGCAGCTTCAAGTGGTACCTGGAGAAC860.11673679923985342No Hit
AGGCCTGGGAATTGGTTTACCATGTAATGACACTTCCATTTTAAAGATTTTA830.11266458531288177No Hit
ACACTTGGGAAGTAGGCATGGTGGGAATATTTACACTAGGGAAATCAGCAAA830.11266458531288177No Hit
GCTTGGGGGTGTGAGATGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTG830.11266458531288177No Hit
AGCTTTGGGGTCGCCCATAGTTGTAGTTTTGGGTTCTGGCAGGTGGAACACC830.11266458531288177No Hit
ACTAGTGGGAAGCCACAGACCGGAAGTATTATTTACAATAAGTATATCTAAC820.11130718067055788No Hit
TAAACCCCATGTTAAGAAAAGCCTGTAAAGCTGACATTCCTAAATTCTGTCA820.11130718067055788No Hit
TAACATGGGAGTGACTAATTTTCCCTTACAGTTCCTGCTTGGTCCCACCCAC820.11130718067055788No Hit
TGTACGCGGGTACGCCCTTGAAAGCACTGGGATTACAGGTGTGAGCCACCAT820.11130718067055788No Hit
TTCCAGGGGAGTTTTTCTTAATTATAACTTCTTAGGGTTAGAAAACAATAAT810.10994977602823403No Hit
CCTAAAGGGGTGCCCCTAGCTTTATATATGGACATGGCAGGCCGATTTGGGA810.10994977602823403No Hit
CTAACCGGGATCGAGGATACTGGCATGGCTGCAATCCACTGACCTTATTTGA810.10994977602823403No Hit
AGTATGGGCATTGGTGCTTTTTGCTTTGTCCTGTGGTAATAAGAAGGATCAT800.10859237138591013No Hit
GATTGAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG800.10859237138591013No Hit
TAAGCAGGGTGCCACGTAATATTTCAGCCATTCAGAGGAAACTGTTTTCTCT800.10859237138591013No Hit
TCAACAGGGGGGCTGTAAGCTGCTGCTTATGTTGAAAAGTGGTTCTTCAGAT800.10859237138591013No Hit
ACAGCTGGCTATGCAATGAATAGATATGCATATTATGTGTGCTACAAATGCA800.10859237138591013No Hit
AGTCTCGGGTGCTCGCGAGGGTGACCTCCTGACCAAGGAGCGGCTGTGCTGT800.10859237138591013No Hit
AGCAAAGGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGG800.10859237138591013No Hit
GCAACAGGGTGGGATATGAAAAGGAAAAATGAGGGAGTTCTTTGGAGGTGAT790.10723496674358626No Hit
GCGTTTGGGCTGGCCCAGTATCTTCCTGACAGCCCCTTAGCACAGGTTACTC790.10723496674358626No Hit
TTATCAGGGGATAATAGGTATCCTAACAAGTTTGATATGACAGCTCATTGTA790.10723496674358626No Hit
TTCTCGGGGAGGAGCTGGCCAGCCAGCCTGATGTGGATGGCTTCCTTGTGGG790.10723496674358626No Hit
CCGTTTGGGATCAAAAGGATTGGATAATAAAAATAAGTTTCTACTGGGTATA780.10587756210126238No Hit
TTAGCCGGGGAAGATGGAAGGAAAAGGTAGCAAAGGAAAGACAGCAGAAATA780.10587756210126238No Hit
AGGACTGGAGCACAGGAATGTTTCTGATTACACACCTCTAAGTCTGGCTGCT780.10587756210126238No Hit
GCTGCTCGGGAGGCTGAGGCAGGAGGATTGTTTGAGCACAGGAGATTGAACT780.10587756210126238No Hit
CGCTGCGGGAATGAAAAACCAGGCATCAGAACAAAAAGAAAAAAGAAAAAAA770.10452015745893851No Hit
GTTCATGGGAAGAAAGCTCATGTGTTATTGTCTCTGGTGGGGGTAATGTGAT770.10452015745893851No Hit
CTGAAAAGGGAGTGATTGTCCAGGGTGTCCATGAGCTCTGTGATCTGGAGGA770.10452015745893851No Hit
TGGCTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA770.10452015745893851No Hit
TCAGATGGGGACTAGAAGGCGAGGCGCCGCGGGACCATGGCGGCGGCGGCGG770.10452015745893851No Hit
GCAGCGGGCTTACCATACAGTACAGTGGTTTCAAAATTTTTATCTGTATTCT770.10452015745893851No Hit
GGCGCTGGGGGTAGGCAATAGAATATATATGTCTGACACTCAAGTAGGGGTA760.10316275281661462No Hit
GATCCCGGGATTCAAGACAGAAAGAACAGCTAGAGCCAAAATACAATTCAAA760.10316275281661462No Hit
AATCAAGGGTGGTGCAGGTCACTACCCACAGAAGAACTGACCAAGAAGAGAG760.10316275281661462No Hit
AGTAGAGGGAAAGCACCTAAGCCTCAAAAACTAGAATTGTCTTAGGAGTTAA760.10316275281661462No Hit
GCTTCCGGGATATTGTTCTGTGTGTAACTGATTTCTATTTTAGGTCCTATTC760.10316275281661462No Hit
AGCTCCGGGATCCCGAGGGAAGTCCTCCAGTCTAAGTCACAGTGCCCTGACA750.10180534817429077No Hit
AAGCAAGGGATCTGAGAAGACATCGGACTGGCAAACGGTAAGTCGTGTTACC750.10180534817429077No Hit
CTAACTGGGTGCTTACCAATTGATGGAAAATGGCAGCTTAGAGAATCTGTGG750.10180534817429077No Hit
AACCATGGGAGGGCATATCTGGAATCTGAAGTTGCTATATCTGAGGAGTTGG750.10180534817429077No Hit
AATAATGGGGTGTGGGAAAGCCTTTAGTGTGCATTCGAACTTAACTACCCAT750.10180534817429077No Hit
TGATGCGGGGATGCTGCAGCTTAAATGATATTATACTTTGAAAACCTCTGCT740.10044794353196687No Hit
TAATGAGGGATGTGAATAAAATACGTATGTTAAATCAAAGATTTAGTAACTC740.10044794353196687No Hit
GTATGTGGGCTTTGCAAACACATTATGCTTTTGCACACACACACCTGTACAC740.10044794353196687No Hit
TAAGCGGGGGGTAACAAATTACCACAGGTTTACTGGTTTATAACAGCATAAT740.10044794353196687No Hit
TATGAGTGGGGCATGATGTGTTTGTGTGTCACAAATCCAGAAAGTGAACTGC740.10044794353196687No Hit
TAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC740.10044794353196687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)