FastQCFastQC Report
Sat 31 Dec 2022
SRR5843977.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843977.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17211
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC2431.41188774620882No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT2131.2375806170472372RNA PCR Primer, Index 17 (95% over 22bp)
TGCTTGGGGTAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAAGCCAT1751.0167915867758992No Hit
CAACCTGGGAGGTACATGTGAGAAACTGCCGAGTAGGTTCCTTCCTCAAAAC1660.9644994480274243No Hit
GAACTTGGGGAGGGGCCAGGGCGCGTCGGAGCCGCTGAGAAAGCGCAGAGAA1620.9412584974725466No Hit
TGGAGGGAACCCTGGGCAAGTTACTTCACCTCTGAGCCTCCGATGCTCTGTG1500.8715356458079135No Hit
CTAACGCGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGA1390.807623031782No Hit
ATTAATGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1390.807623031782No Hit
TGATTTGGGGACAATTAATTGTGAAAGCATTAATTAGAAGTCAAGACAAAAG1380.8018127941432804No Hit
ACAATAGGGCAATTGCTGCATGAAATAAGCTTCATTTGACTAAACATCCATA1380.8018127941432804No Hit
CACTGCGGGATCTGTTGAGATGGCTGTGGTGAGCAACTGAACGAGCCTACGT1370.796002556504561No Hit
CTGACTGGGATTTCTCAGGTACCTGCATTTGATGTTTCAAGTTTCAGCTTCA1340.7785718435884027No Hit
TTATGACGGGTCCACTTAAGAGAACAGACTGGAGACTGCTGGAGGCCCTGAA1270.7379001801173668No Hit
CGCACTGGGAGCCGGAGGGGGCCCGGCGTACCCAGCCCCCAGCCCGACGTGA1230.7146592295624891No Hit
GAATTAGGGAGCTTCATTTGGGAAAATTTCATCTTCACTGTACTTTCCTTCC1190.6914182790076114No Hit
AATGGAAGGGAGGCGAGGGCGCCGCACCCACACCGCGCTGCGCAGTTTTGTT1170.6797978037301725No Hit
TCAAACGGGGCAGCTATTTCACAAGTGGATCGATTGCAGTCAGAGCCTGAAA1150.6681773284527337No Hit
GCCGCCGGCAAGCAGTGGGACTATTATGCCAGAAGAGAGAAAGACCGAGATA1110.644936377897856No Hit
TTTAATGGGCTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGG1070.6216954273429783No Hit
CGTGACGGGTGCATGCTCTATTCTAAGTAGAATGTTCAATGTAACTGACTAA1070.6216954273429783No Hit
TGATATGGGGTGCAGGGTCAGGCTTTGTGAGTCCTCACAGGGAGGGTTAACT1040.6042647144268201No Hit
CGACTAGGGATCTGGAGGGACAGTGGCTGGGGTGCTGCAGCCTAGGTTAGAC1030.5984544767881007No Hit
TAGACGGGAAATTGGTCTAATTCTGTCATTCAGCTAATTGGCCCATTAGCTG1030.5984544767881007No Hit
GTGTTAGGGAGTGTGGAAGGTCTTCTAAATGCTCTCAGGTACACAACAAAAC1000.5810237638719424No Hit
CGGCGTGGGAGCCCTACCAAGTAGAAAAAAAGCAATCAATTTAATTAAAAAG980.5694032885945035No Hit
ATATAAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC950.5519725756783452No Hit
GTCCAGGGGATGCAGTCAATTATTTCTTTTTAAGAGGAGGAGGATTCACTTG930.5403521004009063No Hit
AAATATGGGCGATCTATCGTGGGGAAGTTCATCGAAGAAGTTCTGAAGTTTC910.5287316251234676No Hit
GCCACAGGGACTGGATCACCTGACTCCAGCTAGATTGCCTCTCCTGGACATG900.522921387484748No Hit
TGCCTAGGGGCTTTTTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGA870.5054906745685899No Hit
TAAATAGGGAAGGCCCCTATCAAATATGCATTTTACCATAATTTTATCCCAG840.4880599616524316No Hit
TCTACTGGGATATAACAGGAGCCCTGGCAGCTGTCTCCAGAGGATCAAAGCC800.46481901109755386No Hit
TATGGAGGGATTATTTTATTGCAGAGTTGTTTGTAATAGTAAAAACAAAAAT800.46481901109755386No Hit
ATTTCTGGGGTCGGAAGGTGGCGAGCCTGAGGCGAACAATGGCGGAGCTGGG790.45900877345883445No Hit
AATGTTGGGGAGCATCAAGAACAAAGTATCAAACACAATACTCGAGAAAGGC750.43576782290395677No Hit
GTTTAAGGGGGGCTTTGTGGTTTGCGTCCCTAAAGTGAGCCCACTACTGGTT740.4299575852652373No Hit
GAAACTGGGAGTTTTTTCATGGATTTGTGTTCTTACAGTACTTGAAAATATT730.4241473476265179No Hit
TTATGTGGGACTGGGTAAAGTCCTAAGTGCTTTGCCTGCATTAACTCAATAT730.4241473476265179No Hit
TTAATAGGGTGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTT710.41252687234907903No Hit
TTGTCAGGGGACGCATGCGCATAGCTAACCGCACCCGGTTCAGCTCGCCTTT700.4067166347103596No Hit
TTATCTGGGATTGTAAAAAGAGAACATGCATTAACATCAGGCACGATAAAAG700.4067166347103596No Hit
CCAACAGGGGCCTAAAAGTATGCTTTCACTGTCAGCAGTTTTAAAGGTGAAA680.39509615943292087No Hit
TATGCTGGGCACCTGCAATAAAACAGTCTTGTCGGCCAGCTGCCCAGGGGAC680.39509615943292087No Hit
GGCACTGGGGTGTGTATGTGTGTGTATATATAGATGTATATATATACACACA680.39509615943292087No Hit
ATGAGCGGGGAAGAAAATGGTTTTTTGATCAGACTGGGACAGAATTTAAGAC670.38928592179420135No Hit
CTTGTTACGGGCAGCAGAAAAAGTGACATTTAATGGAAAGCAAAACAAATCA610.35442449596188486No Hit
GGGAACCGGGGAATCCAATGATACAGATTTGATTACTAAGTTTGAACTGTTA600.3486142583231654No Hit
GGCCATGGGGTCTGCACAACTGCATGCACTTCTCTCCCCATCGCTCCACAAC600.3486142583231654No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAG550.3195630701295683No Hit
GAATAGGGGGGCCCCTGGGACCCTCCGGGCCGGGCGGTTTGGCCCCTTAGCG530.3079425948521295No Hit
GCAAGTGGGAAGATGAGATCAGGAGGGAAATTGGGTGCTCTGCCTCAGGCTG460.2672709313810935No Hit
CTTAGTGGGGACGCTCTTCCGTTCTGGTAAAGGGTTGGCAGCTCACAGAGAG420.2440299808262158No Hit
ATCACTGGGGTAAGAGCATAGAGAATATTAATAATGGCTTATAGAGAGAGAC410.23821974318749636No Hit
ACTATAAGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTC410.23821974318749636No Hit
GTAGTAGGGGGGCCGAGAGGTGGTTACATTCGTTGAAGGACACCAGCTGCGG410.23821974318749636No Hit
CCTTCTGGGATTACTTTTGTAAAAACACTGGTTAATTTGTATAAGATATTAT400.23240950554877693No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGA400.23240950554877693No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCTTATAC340.19754807971646043No Hit
CACTAGGGGATTCATACATCAAGCAGCATTTTTTTCACTCTCCTTAGAATTG310.18011736680030213No Hit
ATCATTGGGATGATCTTTGTAACGCCTGAAGTTAGAGCAATTTATTAACTTT280.16268665388414386No Hit
AGCTTTGGGACCACTTTTATTCAATGCAGTACTGAAAGTTCTAGCCCAAGCA270.15687641624542442No Hit
CTGCCCGGGAATGACCCCCATTTGTGTGACTTCATTGAGACACATTACCTGA250.1452559409679856No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCT220.12782522805182733No Hit
TTGACTGGGCTAACGCGGGGGGGTGATTATGAATAGAACTACTATAAATATT210.1220149904131079No Hit
CGTAACGGAGATATAGGAATGAAAAGTACCAGCTGAACTAGGAAAGGGGAAG210.1220149904131079No Hit
TCTCAAGGGAGACAAACAGTAGACAAATGCAGCAGCAAGTCAATTGTATTAG200.11620475277438846No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGAACTCGTATGC180.10458427749694962No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)