FastQCFastQC Report
Sat 31 Dec 2022
SRR5843976.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843976.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17224
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC2451.42243381328379No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT2121.2308406874129123RNA PCR Primer, Index 17 (95% over 22bp)
CAACCTGGGAGGTACATGTGAGAAACTGCCGAGTAGGTTCCTTCCTCAAAAC1731.0044124477473293No Hit
TTATGACGGGTCCACTTAAGAGAACAGACTGGAGACTGCTGGAGGCCCTGAA1480.8592661402693916No Hit
TGCTTGGGGTAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAAGCCAT1450.8418485833720389No Hit
TGGAGGGAACCCTGGGCAAGTTACTTCACCTCTGAGCCTCCGATGCTCTGTG1420.8244310264746865No Hit
CTAACGCGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGA1420.8244310264746865No Hit
TCAAACGGGGCAGCTATTTCACAAGTGGATCGATTGCAGTCAGAGCCTGAAA1410.818625174175569No Hit
ATTAATGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1410.818625174175569No Hit
GAACTTGGGGAGGGGCCAGGGCGCGTCGGAGCCGCTGAGAAAGCGCAGAGAA1370.7954017649790989No Hit
ACAATAGGGCAATTGCTGCATGAAATAAGCTTCATTTGACTAAACATCCATA1360.7895959126799814No Hit
TGATTTGGGGACAATTAATTGTGAAAGCATTAATTAGAAGTCAAGACAAAAG1270.7373432419879238No Hit
CTGACTGGGATTTCTCAGGTACCTGCATTTGATGTTTCAAGTTTCAGCTTCA1270.7373432419879238No Hit
ATATAAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1210.7025081281932187No Hit
CGCACTGGGAGCCGGAGGGGGCCCGGCGTACCCAGCCCCCAGCCCGACGTGA1190.6908964235949837No Hit
CACTGCGGGATCTGTTGAGATGGCTGTGGTGAGCAACTGAACGAGCCTACGT1180.6850905712958663No Hit
GAATTAGGGAGCTTCATTTGGGAAAATTTCATCTTCACTGTACTTTCCTTCC1120.6502554575011612No Hit
TTAATAGGGTGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTT1110.6444496052020436No Hit
GCCGCCGGCAAGCAGTGGGACTATTATGCCAGAAGAGAGAAAGACCGAGATA1070.6212261960055736No Hit
CGGCGTGGGAGCCCTACCAAGTAGAAAAAAAGCAATCAATTTAATTAAAAAG1020.5921969345099861No Hit
TAAATAGGGAAGGCCCCTATCAAATATGCATTTTACCATAATTTTATCCCAG1010.5863910822108686No Hit
CGTGACGGGTGCATGCTCTATTCTAAGTAGAATGTTCAATGTAACTGACTAA1010.5863910822108686No Hit
GCCACAGGGACTGGATCACCTGACTCCAGCTAGATTGCCTCTCCTGGACATG1010.5863910822108686No Hit
AATGGAAGGGAGGCGAGGGCGCCGCACCCACACCGCGCTGCGCAGTTTTGTT1000.5805852299117511No Hit
TGATATGGGGTGCAGGGTCAGGCTTTGTGAGTCCTCACAGGGAGGGTTAACT1000.5805852299117511No Hit
TAGACGGGAAATTGGTCTAATTCTGTCATTCAGCTAATTGGCCCATTAGCTG1000.5805852299117511No Hit
GTCCAGGGGATGCAGTCAATTATTTCTTTTTAAGAGGAGGAGGATTCACTTG990.5747793776126335No Hit
GTGTTAGGGAGTGTGGAAGGTCTTCTAAATGCTCTCAGGTACACAACAAAAC960.5573618207152811No Hit
AAATATGGGCGATCTATCGTGGGGAAGTTCATCGAAGAAGTTCTGAAGTTTC940.5457501161170459No Hit
CGACTAGGGATCTGGAGGGACAGTGGCTGGGGTGCTGCAGCCTAGGTTAGAC940.5457501161170459No Hit
TTATGTGGGACTGGGTAAAGTCCTAAGTGCTTTGCCTGCATTAACTCAATAT910.5283325592196935No Hit
TTTAATGGGCTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGG900.522526706920576No Hit
GAAACTGGGAGTTTTTTCATGGATTTGTGTTCTTACAGTACTTGAAAATATT880.5109150023223409No Hit
AATGTTGGGGAGCATCAAGAACAAAGTATCAAACACAATACTCGAGAAAGGC790.45866233163028336No Hit
TATGCTGGGCACCTGCAATAAAACAGTCTTGTCGGCCAGCTGCCCAGGGGAC770.4470506270320483No Hit
GTTTAAGGGGGGCTTTGTGGTTTGCGTCCCTAAAGTGAGCCCACTACTGGTT740.4296330701346958No Hit
TATGGAGGGATTATTTTATTGCAGAGTTGTTTGTAATAGTAAAAACAAAAAT730.4238272178355783No Hit
TCTACTGGGATATAACAGGAGCCCTGGCAGCTGTCTCCAGAGGATCAAAGCC710.41221551323734323No Hit
ATGAGCGGGGAAGAAAATGGTTTTTTGATCAGACTGGGACAGAATTTAAGAC660.3831862517417557No Hit
TTGTCAGGGGACGCATGCGCATAGCTAACCGCACCCGGTTCAGCTCGCCTTT640.3715745471435207No Hit
ATTTCTGGGGTCGGAAGGTGGCGAGCCTGAGGCGAACAATGGCGGAGCTGGG630.36576869484440316No Hit
TTATCTGGGATTGTAAAAAGAGAACATGCATTAACATCAGGCACGATAAAAG620.35996284254528566No Hit
CTTAGTGGGGACGCTCTTCCGTTCTGGTAAAGGGTTGGCAGCTCACAGAGAG610.35415699024616815No Hit
GAATAGGGGGGCCCCTGGGACCCTCCGGGCCGGGCGGTTTGGCCCCTTAGCG570.33093358104969806No Hit
TGCCTAGGGGCTTTTTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGA570.33093358104969806No Hit
CCAACAGGGGCCTAAAAGTATGCTTTCACTGTCAGCAGTTTTAAAGGTGAAA560.3251277287505806No Hit
GCAAGTGGGAAGATGAGATCAGGAGGGAAATTGGGTGCTCTGCCTCAGGCTG560.3251277287505806No Hit
GGGAACCGGGGAATCCAATGATACAGATTTGATTACTAAGTTTGAACTGTTA550.3193218764514631No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAG550.3193218764514631No Hit
GGCCATGGGGTCTGCACAACTGCATGCACTTCTCTCCCCATCGCTCCACAAC520.3019043195541105No Hit
GGCACTGGGGTGTGTATGTGTGTGTATATATAGATGTATATATATACACACA510.29609846725499306No Hit
CCTTCTGGGATTACTTTTGTAAAAACACTGGTTAATTTGTATAAGATATTAT510.29609846725499306No Hit
CTTGTTACGGGCAGCAGAAAAAGTGACATTTAATGGAAAGCAAAACAAATCA500.29029261495587555No Hit
GTAGTAGGGGGGCCGAGAGGTGGTTACATTCGTTGAAGGACACCAGCTGCGG410.23803994426381792No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGA410.23803994426381792No Hit
ATCATTGGGATGATCTTTGTAACGCCTGAAGTTAGAGCAATTTATTAACTTT400.23223409196470043No Hit
ATCACTGGGGTAAGAGCATAGAGAATATTAATAATGGCTTATAGAGAGAGAC350.20320483046911286No Hit
AGCTTTGGGACCACTTTTATTCAATGCAGTACTGAAAGTTCTAGCCCAAGCA310.17998142127264283No Hit
CTGCCCGGGAATGACCCCCATTTGTGTGACTTCATTGAGACACATTACCTGA300.17417556897352532No Hit
CACTAGGGGATTCATACATCAAGCAGCATTTTTTTCACTCTCCTTAGAATTG280.1625638643752903No Hit
TTGACTGGGCTAACGCGGGGGGGTGATTATGAATAGAACTACTATAAATATT260.15095215977705526No Hit
GAGTATGGGGACTCTAGGCCGAGAAGAAAAAGTGGGCTCACCACAAGGAGCA250.14514630747793777No Hit
ACTTCGGGGGACCTGGTGCGGTGGCTCACGCCTATAATCCCAGCACTTTGGG230.13353460287970273No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCTTATAC230.13353460287970273No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGAT210.12192289828146773No Hit
ACTATAAGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTC210.12192289828146773No Hit
CAATTAGGGCGATCTTCAATGGGGGAAGAAAGAATACATAAAGACTTTCAAT190.1103111936832327No Hit
AGCAAGGGGACGCAGATATCTACATAGATATGCTTTGCTGAGAAGTTAGGTC190.1103111936832327No Hit
CCCACTGCCCACTGGCCGGCCTCTTCCAGCCGCCCCCACCCCCCCGGACCCC190.1103111936832327No Hit
GATGCGGGAGTTCTTAGAAGCTCACGCCTTAGTTTGAAACAGATTCTCCACG180.10450534138411519No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)