FastQCFastQC Report
Sat 31 Dec 2022
SRR5843975.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843975.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17602
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGC2381.3521190773775706No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCTCGTATGCCGTCT2321.3180320418134301RNA PCR Primer, Index 17 (95% over 22bp)
CAACCTGGGAGGTACATGTGAGAAACTGCCGAGTAGGTTCCTTCCTCAAAAC1791.0169298943301897No Hit
TTATGACGGGTCCACTTAAGAGAACAGACTGGAGACTGCTGGAGGCCCTGAA1720.9771616861720259No Hit
TGCTTGGGGTAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAAGCCAT1500.8521758891035109No Hit
GAACTTGGGGAGGGGCCAGGGCGCGTCGGAGCCGCTGAGAAAGCGCAGAGAA1330.755595955005113No Hit
TGGAGGGAACCCTGGGCAAGTTACTTCACCTCTGAGCCTCCGATGCTCTGTG1320.7499147824110897No Hit
TCAAACGGGGCAGCTATTTCACAAGTGGATCGATTGCAGTCAGAGCCTGAAA1300.7385524372230428No Hit
GCCGCCGGCAAGCAGTGGGACTATTATGCCAGAAGAGAGAAAGACCGAGATA1290.7328712646290194No Hit
CTAACGCGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGA1290.7328712646290194No Hit
TGATTTGGGGACAATTAATTGTGAAAGCATTAATTAGAAGTCAAGACAAAAG1270.7215089194409726No Hit
CTGACTGGGATTTCTCAGGTACCTGCATTTGATGTTTCAAGTTTCAGCTTCA1250.7101465742529258No Hit
CACTGCGGGATCTGTTGAGATGGCTGTGGTGAGCAACTGAACGAGCCTACGT1250.7101465742529258No Hit
ATATAAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1240.7044654016589024No Hit
AAATATGGGCGATCTATCGTGGGGAAGTTCATCGAAGAAGTTCTGAAGTTTC1230.698784229064879No Hit
TAGACGGGAAATTGGTCTAATTCTGTCATTCAGCTAATTGGCCCATTAGCTG1200.6817407112828088No Hit
GAATTAGGGAGCTTCATTTGGGAAAATTTCATCTTCACTGTACTTTCCTTCC1180.6703783660947619No Hit
ACAATAGGGCAATTGCTGCATGAAATAAGCTTCATTTGACTAAACATCCATA1160.6590160209067151No Hit
CGACTAGGGATCTGGAGGGACAGTGGCTGGGGTGCTGCAGCCTAGGTTAGAC1150.6533348483126917No Hit
CGCACTGGGAGCCGGAGGGGGCCCGGCGTACCCAGCCCCCAGCCCGACGTGA1120.6362913305306216No Hit
ATTAATGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAAC1110.6306101579365981No Hit
CGTGACGGGTGCATGCTCTATTCTAAGTAGAATGTTCAATGTAACTGACTAA1090.6192478127485513No Hit
TAAATAGGGAAGGCCCCTATCAAATATGCATTTTACCATAATTTTATCCCAG1080.6135666401545279No Hit
GCCACAGGGACTGGATCACCTGACTCCAGCTAGATTGCCTCTCCTGGACATG1070.6078854675605044No Hit
CGGCGTGGGAGCCCTACCAAGTAGAAAAAAAGCAATCAATTTAATTAAAAAG1030.5851607771844108No Hit
TTAATAGGGTGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATTCATCTT1010.573798431996364No Hit
TTATCTGGGATTGTAAAAAGAGAACATGCATTAACATCAGGCACGATAAAAG980.5567549142142939No Hit
TATGGAGGGATTATTTTATTGCAGAGTTGTTTGTAATAGTAAAAACAAAAAT970.5510737416202705No Hit
AATGGAAGGGAGGCGAGGGCGCCGCACCCACACCGCGCTGCGCAGTTTTGTT950.5397113964322237No Hit
TTTAATGGGCTCTTCCTGCTCTCCATCATGGCGCAGGATCAAGGTGAAAAGG920.5226678786501534No Hit
GAAACTGGGAGTTTTTTCATGGATTTGTGTTCTTACAGTACTTGAAAATATT920.5226678786501534No Hit
TGATATGGGGTGCAGGGTCAGGCTTTGTGAGTCCTCACAGGGAGGGTTAACT870.49426201568003636No Hit
TCTACTGGGATATAACAGGAGCCCTGGCAGCTGTCTCCAGAGGATCAAAGCC850.48289967049198956No Hit
GTCCAGGGGATGCAGTCAATTATTTCTTTTTAAGAGGAGGAGGATTCACTTG800.4544938075218725No Hit
GTGTTAGGGAGTGTGGAAGGTCTTCTAAATGCTCTCAGGTACACAACAAAAC800.4544938075218725No Hit
TTATGTGGGACTGGGTAAAGTCCTAAGTGCTTTGCCTGCATTAACTCAATAT740.42040677195773213No Hit
GTTTAAGGGGGGCTTTGTGGTTTGCGTCCCTAAAGTGAGCCCACTACTGGTT700.3976820815816385No Hit
ATGAGCGGGGAAGAAAATGGTTTTTTGATCAGACTGGGACAGAATTTAAGAC700.3976820815816385No Hit
ATTTCTGGGGTCGGAAGGTGGCGAGCCTGAGGCGAACAATGGCGGAGCTGGG690.392000908987615No Hit
GCAAGTGGGAAGATGAGATCAGGAGGGAAATTGGGTGCTCTGCCTCAGGCTG660.37495739120554483No Hit
TATGCTGGGCACCTGCAATAAAACAGTCTTGTCGGCCAGCTGCCCAGGGGAC620.3522327008294512No Hit
CTTGTTACGGGCAGCAGAAAAAGTGACATTTAATGGAAAGCAAAACAAATCA620.3522327008294512No Hit
CCAACAGGGGCCTAAAAGTATGCTTTCACTGTCAGCAGTTTTAAAGGTGAAA590.33518918304738093No Hit
GGGAACCGGGGAATCCAATGATACAGATTTGATTACTAAGTTTGAACTGTTA590.33518918304738093No Hit
TTGTCAGGGGACGCATGCGCATAGCTAACCGCACCCGGTTCAGCTCGCCTTT590.33518918304738093No Hit
GGCACTGGGGTGTGTATGTGTGTGTATATATAGATGTATATATATACACACA580.32950801045335754No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAG570.3238268378593342No Hit
AATGTTGGGGAGCATCAAGAACAAAGTATCAAACACAATACTCGAGAAAGGC560.3181456652653108No Hit
TGCCTAGGGGCTTTTTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGA550.3124644926712874No Hit
GTAGTAGGGGGGCCGAGAGGTGGTTACATTCGTTGAAGGACACCAGCTGCGG540.30678332007726394No Hit
CTTAGTGGGGACGCTCTTCCGTTCTGGTAAAGGGTTGGCAGCTCACAGAGAG520.29542097488921715No Hit
GAATAGGGGGGCCCCTGGGACCCTCCGGGCCGGGCGGTTTGGCCCCTTAGCG500.28405862970117035No Hit
GGCCATGGGGTCTGCACAACTGCATGCACTTCTCTCCCCATCGCTCCACAAC460.2613339393250767No Hit
CCTTCTGGGATTACTTTTGTAAAAACACTGGTTAATTTGTATAAGATATTAT450.2556527667310533No Hit
ATCACTGGGGTAAGAGCATAGAGAATATTAATAATGGCTTATAGAGAGAGAC450.2556527667310533No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGA420.23860924894898308No Hit
ACTATAAGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTC380.21588455857288946No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCTTATAC340.19315986819679581No Hit
ATCATTGGGATGATCTTTGTAACGCCTGAAGTTAGAGCAATTTATTAACTTT340.19315986819679581No Hit
AGCTTTGGGACCACTTTTATTCAATGCAGTACTGAAAGTTCTAGCCCAAGCA320.181797523008749No Hit
CTGCCCGGGAATGACCCCCATTTGTGTGACTTCATTGAGACACATTACCTGA310.1761163504147256No Hit
CACTAGGGGATTCATACATCAAGCAGCATTTTTTTCACTCTCCTTAGAATTG290.16475400522667877No Hit
CGTAACGGAGATATAGGAATGAAAAGTACCAGCTGAACTAGGAAAGGGGAAG290.16475400522667877No Hit
GAGTATGGGGACTCTAGGCCGAGAAGAAAAAGTGGGCTCACCACAAGGAGCA280.1590728326326554No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGAT270.15339166003863197No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTTGTACAGATCCTGTCTCT210.11930462447449154No Hit
GATGCGGGAGTTCTTAGAAGCTCACGCCTTAGTTTGAAACAGATTCTCCACG190.10794227928644473No Hit
AGCAAGGGGACGCAGATATCTACATAGATATGCTTTGCTGAGAAGTTAGGTC190.10794227928644473No Hit
CAATTAGGGCGATCTTCAATGGGGGAAGAAAGAATACATAAAGACTTTCAAT180.10226110669242132No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)