FastQCFastQC Report
Sat 31 Dec 2022
SRR5843965.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843965.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33051
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTGGGAAGCAGTGTGTAGTGGGAAATTTATAGCACTAAATGCCCACAA3190.9651750325254909No Hit
AAGCATGGGTGTTTGCAATAATGGCCTCCAATACTGAAACAACCAAAAGCAG2650.8017911712202354No Hit
TGCGCGGGGGCCAAAAGGTAGAAACAATTCAAATGTCCATCAACTGATAAAG2620.7927142900366101No Hit
AGCAATGGGCGGTCAGTCCCCACATCAACTGTCTTTTACCCAAGTGATGGCG2590.7836374088529848No Hit
CAAGTGTGGGAAGGATGCAAAGGTCGCTCAAGAGAAAAAGTTGAAAAATACA2470.7473298841184836No Hit
ATTGCTGGGTACACTCCAGGCCTCCCATCCTCTGCATCTATACCCCACAGGA2410.7291761217512329No Hit
GTTAGCGGGAAGATTCAGGAGTATTGCTATGACAACATTCATTTCATGAAAG2380.7200992405676077No Hit
CTTAGTGGGTACAATCAATTAACATTTTATACAAAAGATTGAATTTAAGAGA2370.7170736135063992No Hit
GCTTGGAGGGACATATATCAGTGAGAAGTCCGTAAAAACTTACTCTGTTTAT2350.7110223593839824No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGC2310.6989198511391486No Hit
AAAGCTGGGGGAGTGGGGACTTGATCCTGGGGGCAGGGCCTTGGACTTAGGG2290.6928685970167318No Hit
TCAACCGGGCGATTTCCTAAAGTACCTGGCAAAGAATTCTGCAACTTTGTTC2290.6928685970167318No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGCCGTCT2290.6928685970167318No Hit
AATATGGGATGACAGTTTGACTTGAATGCAACAGCAGGAAAATTTTGCAAGT2250.680766088771898No Hit
TACCTGGGTGAGGTTTTGGGTATGAATGTTCCCATCACCCAAGAACTAAGCA2240.6777404617106895No Hit
TGCATATGGGACAGTTCTTGGGAAAGAAATGAATGAGAAGTTTGGAATGTCT2130.6444585640373968No Hit
AATCAAGGGCACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC2120.6414329369761883No Hit
CAATGTGGGCCTGTTTGCAGCATGCTGTGTGCAAACACTCAGTAATTTGGAG2120.6414329369761883No Hit
TGCCAGGTTTACAAACTCAGACCCTTTTAAAAGTTGTTTAAATTGTTTTGTT2110.63840730991498No Hit
TCTGCTGGGGTGCAAAAACACCTTTTTGAACAGCAAAGATTTCACCCACCAA2030.6142022934253124No Hit
CTAGCCTGGGCAACAGAGTAAGACAAAAAAATATATATATATTGAAAATCAA2030.6142022934253124No Hit
AGAATTGGGCTTCCCAGACTTTCTCAAGCTCCTCCTCACTGCCCTTCCTCCC1990.6020997851804787No Hit
GCTGCGGGGTCTCCTATCACATGCACTCTGAATCAGGGTTTTTCTATAAGAA1920.5809203957520196No Hit
GTAATTGGGGTGGCACTGGGCGCGGGCGTGACGGCGCTCAGCCATCCCCTGC1900.5748691416296028No Hit
GTACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1850.5597410063235606No Hit
AGTCAAGGGTCCTATCAATGCCATATGTGTTAATTCCACCCACATTTTTACT1770.5355359898338931No Hit
CTAACTGGGGGTGGGATGCACACTTGGCCTGGCCTTTCCCAGAGGCAGGAGC1730.5234334815890593No Hit
TCTTAGGGCAGCTCAACCTCTAAAGCTCATGTTTTTTCTTTCTCTCTGTAAG1730.5234334815890593No Hit
AAAAGTGGGAAGGCAGAAATAAAGATGTTCTTTGAAACCACTGAGAACAAAG1720.5204078545278509No Hit
CGCTAAGGGGAGCGCTGCGGGGCGTCTCCCTCGCAGTCCCGCCCGCCCCTCC1660.5022540921606004No Hit
TAAGCAGGGCTGACACCCAGTGGATCACCAAACCAGTCCACAAGCACAGGGA1650.4992284650993918No Hit
TGTAAAGGGTGTTCCCATGAAGGGAACACAATTACGTTATTATTTTTACAGT1630.493177210976975No Hit
ACTGTGGGGTATGTTGGAGGAGGGCTTACTGATGCGTGCTAAGACCGATTTC1590.4810747027321412No Hit
CTAAAGGGGATCCCCATTCCCCCTCTTGGGCCTCAGTTTCCCGACTTGCAAA1570.4750234486097244No Hit
TATGCCGGGTGCTACGTCCAGGGTGAGAATAAAGACTTCTCCTCAGGACTCT1530.4629209403648906No Hit
CTTTATCGGGGTGCTTGGAGTTTAGGAACCCCCAGGATGGCTCTTAGCTGCA1530.4629209403648906No Hit
ATATTAGGGACAGTTTCTTTGGAAATACGTTAAAGATAGTGGCAATTTCATA1520.4598953133036822No Hit
TACATGGGGCACCGTTCTGAACTCCCAGATCTGAGAAGGAACTAGTGTTGGT1470.44476717799764No Hit
CCAACAGGGTAAGCCAGGCACCGAATGACAAACTTTGTTTGTTCTCACTTAT1470.44476717799764No Hit
GTGCCGGGGCCTAATGGGAGTGCGAGAGCTCTGCACCAGGCACCAGGTTCTC1440.4356902968140147No Hit
GTGTTTGGGAGGGAATGGGTTCCCTTATTAAGATGTTCTTGCATCCATGTAA1420.4296390426915978No Hit
ACTATTGGGTCGGCCGGGGTATGTGGTGATGCCGCCTCTAATTGACATGGGG1390.42056216150797254No Hit
TAAGTTGGGAGGTGGGAATTGAACAATGAGATCACATGGACACAGGAAGGGG1380.41753653444676403No Hit
AGCTCCGGGGCTACACTGAGCACCAGGTTGTCGCCTCTGACTTTAACAGCAT1370.4145109073855556No Hit
AGGTGCGGGAACAAAATGTCTTCATACTTTAGGGAAACGAATACACTGTATA1360.4114852803243472No Hit
TTGCAGGGATTCACCTGAGATTGCATACATGGCTTTGGAAGCAGGCACTGGA1360.4114852803243472No Hit
TTGACGGGGGAGCTCAACTGCTGTGTTTAAAATACACATTTTAAATCCCTCT1350.4084596532631388No Hit
GTTGCAGATCCCTCATGGCTTGGCAGTCCTTGCAATAGTAGGTGAGTTCTCA1340.40543402620193036No Hit
CATCTGGGGCTCAGGGAGCTGAATATTATGGCAGCCAAGGAAATTGAGGTTG1330.4024083991407219No Hit
CACCGGATTTGTGATATCTAAAAATCAAATCAATTGAACTGATTGAACATAG1320.39938277207951345No Hit
CAGGCTGGGGTGCAGTGGCGTGATCTTGGCTCACTGCGGCCTCCGCCTCCCA1310.39635714501830505No Hit
CTCGGTGGGTATTATTCATGAGTTCTCTATGTAGAATTAGGATTCTGACTAA1270.38425463677347127No Hit
GAACATGGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT1260.38122900971226287No Hit
GCGCGGGGTGGGCTTCTTGGCACTATAGTCATGGGTATGGACTACGTTAGAA1260.38122900971226287No Hit
ATGCGAGGGCGATCTGTAGGAGGGTGAAGTGAAGATGTTGGAAAGACAGAGG1250.3782033826510544No Hit
GCATAAGGGACAACCAAGTGTTGTGTTTTATGTTTTAGGTTTTTATGAAACT1240.375177755589846No Hit
ACCCTTGGGTGAATTCTTTGACTTGTGCTCATTCTTTCTGAACTTATTGAGG1210.3661008744062207No Hit
CTGTTAGGGTCTATCTGCAGAGGTATTCAGTTCCTGGCACAGGGGACTAGGG1190.36004962028380383No Hit
ATTCGAGGGAAGGTTTAGACGAGGCACTTCATGGGTTCAAGGAAAGTACATT1160.3509727391001785No Hit
TATAGAGGGGCCTTTTACATGTTGTCTAATTATCATTTTTCCCCAAATTTTG1150.3479471120389701No Hit
AAGTGGGTGATGCCCATCTCTTATGAAAGTTATAGAACATAGATGATCAATA1140.34492148497776165No Hit
GTTGCCGGCACAGAGAATCCCATCTACAATAGCTACAAAAATTAAAATACTT1110.33584460379413633No Hit
CTCACAAGGGGGATCTGACTCAGGGTGATTGGAGAGATTGAAGAGTGAAGTC1090.32979334967171947No Hit
TAAAAGGGGGGGATTATAGGTATGAGCCACCGTGCCCAGCCAGTAGTTATAA1090.32979334967171947No Hit
ATGTAAGGGTGGTAGAACTGAATGATCATTAAATTAGAACAGAAACAGGGGT1080.326767722610511No Hit
CGACTCGGGGGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGTGAAG1070.32374209554930256No Hit
ATGAACGGGGGGACAAAGGAGTGGCCGTGAGGTCATCCTCAAGGTCCTGGGG1060.32071646848809415No Hit
AAACCAGGGCGGCTCACTGCAACCTCCGCCTCCTGGGTTCAGGTGATTCTCC1050.3176908414268857No Hit
TAACCTGGGTCAGGACTGAAATTTCTGGGCCCCAGGAGATCAAATGCAAAAC1040.3146652143656773No Hit
GGATCCGGGCGGTTTAATTGGAGTCTTAGAACAGTTGTCTTTGAGAAATTCT1030.31163958730446883No Hit
GACCGAGGGCTTCCGATCTTTGAGTTGGGGGGATTTTGAGTTTACTTGTCCC1030.31163958730446883No Hit
GCATATAGGGCAGACGAAAGAAGGTAAGGGCCGCTTGGTCTTAAGATATTTG1020.30861396024326043No Hit
ATGTTAGGGTTATCGTAGCAAGGTGCTCTTCTGGGACAACGCGTACGAGTTC1020.30861396024326043No Hit
ACAGTAGGGGGCATGATGACAGGCACCTGTAATCCCAGCTACTCAGGAGGCT1020.30861396024326043No Hit
GGTCTAGGGAGGGTTGGGGGTGGGTGGGAAAGTGAGTACTGTGTCATAGTGC980.29651145199842666No Hit
TTTAAGGGGGGTGGCGATAAAAATGTACCCATAAATTTAAAGTCCATTCTGT980.29651145199842666No Hit
TTTCAAGGGGAATATGGGAAGCAAGTCTGCCTAACTTCAAGTGGCTTGCACT970.29348582493721825No Hit
AATTACGGGGACCCTGTATTAAAGCTTTTGGACGACAATGGGAACATTGCTG960.2904601978760098No Hit
TTGCCTGGGTGCGTATTTCCTCTACACACATCACCCCCCTTCTATAATCTTA960.2904601978760098No Hit
CTACGTGGGTGAATACAATACTCATCTAGGATGTAACAGTGAAGCTGAGTAA950.2874345708148014No Hit
CAACATGGGAGGGCTTATCAATCTAAGCTCTATTGACATTTTGGGCCTGGTA950.2874345708148014No Hit
TGTAACGGGGCATTAAAAAATGGCTTTTCTTTAGGAAAGTATAATCAATTAT920.2783576896311761No Hit
CGAATAGGGAGGGATTCTGTGTCTTTGTGTCATAACTTTGATGGGGGCAGTG910.2753320625699676No Hit
GGGTCAGGGACGGCCGAGCCCTGCAGGCCTCTGCCCTGAAGGCCTGGGGCGG900.2723064355087592No Hit
AATCCAGGGTGGGAAAGATGCTCAGGGTTCACCATTCTGTTCAGTGGACAAT890.26928080844755076No Hit
AGTATTGGGGCATAAAATTAACTGTAGTATGTACTGCACTACTGTAATAATT880.2662551813863423No Hit
GAATCCGGGGGGGAGTAGAGAGAAAACAGGCGAGAACAAGCACTCTAATTCC880.2662551813863423No Hit
TCTCTTGGGCCCTGGAGGCAGGCTGTACAGAGACATGAGTCAGCTCTCCATC860.26020392726392544No Hit
GTTTTTGGGATCCATGTAACGGTGCATCAATATATTGCTATATAAATATGTC850.25717830020271704No Hit
TTGTAGGGGTACATGATCAGACACCAGAGGGTTGGGCATTCTTGCAATACCT830.2511270460803002No Hit
TGATGAGGGTGCTTCTAGTAGACCTTAATCTAGCATAGTTGAACCTCTGAAT830.2511270460803002No Hit
CAGTTGGGGTCACTTGTACAGTCCACCTTTTGATACATGCCATGCCTTTGAT830.2511270460803002No Hit
TTTGCCGGGTACAAACTGACAGTAGTTACATTTCAAACCTATCTCCTGCTCT820.2481014190190917No Hit
TGAGAAGGGTGTGCTGGGCAAGTGCTGTGCATTCATAAACCTTCTGTCAATC820.2481014190190917No Hit
CTTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCCCCTCAGGAACTCTT820.2481014190190917No Hit
ATTAGAGGGAGCAGAATGAAAAAATATGTAAACAAATTATCCAATACTTACA800.24205016489667483No Hit
TAGTAAGGGGCTTCCACCTCCTTGTAATGAGGTGGGGTAGGAAAGCCTTCAG780.23599891077425794No Hit
AGCATCGGGGCACCACGCCCAGCTAATTTTTGTGGTTTTAGTAGAGAAAGGG760.2299476566518411No Hit
TGGTTGGGATATAACCTCAGTCCAAAGTGGTGATTGATTTGAGTATTTGAAA740.22389640252942425No Hit
CATCTAGGGCAGTACGGGGATTGGCAAGAACGATGCCATCGGCAAAGTCTTT740.22389640252942425No Hit
TAATAAGGGCCACCTTAACAATCCTTCCAGCTACGTATCTTAAAGGATGTGA730.22087077546821576No Hit
AAATCAGGGTATGAAAGATGCTATATTCTATTTCAGCAGGGCCAATATGTGA720.21784514840700736No Hit
GGTTATGGGGTGACTGACCATGTCTGACTGCCACTCCTTTTTGCAGGAGCAT690.20876826722338201No Hit
AGGTAGGGGTCTACGGGAGGGATTCATATCTGTTTCATTTGGAGGATTTTCT670.20271701310096518No Hit
GGTCATGGGCCTGGCTAATTTCTGTATTTTTAGTAGAGACAGGGTTTCACCA660.19969138603975672No Hit
TGAACTGGGAGGGTCTTCAAGACCCTTCAAGACCCATGGCTAATAATTAAGG660.19969138603975672No Hit
ACGGTTGGGATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGT650.19666575897854832No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGG640.1936401319173399No Hit
ATCTTAGGGTGGGTCAGAGAAAACTTCTTGACTAAAACTGGTCAGAACTCAT630.19061450485613143No Hit
ATTCCTGGGATATGTCTGTGTGCATATAAGTGAAAAGTGGTCAAACAAGAGT630.19061450485613143No Hit
CATCAAGGGGCCCTCGGCTTACTTCTCTTCCTTCTCTCCTTAATGACATTAA620.187588877794923No Hit
TTGTTTGGGCAGCAGCAGCCTGATGGAAGTGATGGGGAGATACAATGTGTTG620.187588877794923No Hit
AGAAACGGATTGGAATAAAGAAAAATATATAGGACTCATAAAGAGCTGAAAT610.18456325073371457No Hit
TACATTGGGCATATGTGCAGATATTACACTGAAAAAATTGTATCTGTTCCTA610.18456325073371457No Hit
TTAACTGGGTCTGTATGTGTTCTGTGTCCCTTGCATGTGTGCGTGTTAGAGT600.18153762367250612No Hit
CTAGCAGGGGAACGCCCCGATGGTGTGTTACTGACTTTTGGGGGCCAGACTG600.18153762367250612No Hit
AGTCGAGGGACCCTGTCTTAATAAGGACTTCTGGGATCACGCTGAGCCGCAG570.17246074248888082No Hit
CTCAGTGGGTCCTCTCTTCTCCCTGCTACATTCACTACCAGATTTTTATGCT570.17246074248888082No Hit
GACACTGGGTGCGCCCTGAAGGCAGAGTCCAGGGACCTTGACCCAGCCCCTC560.16943511542767237No Hit
TCACTCGGGCTAATAGTATCACTTTGATTTTTCTTTTTCCTCCCTTAGAAAG560.16943511542767237No Hit
CTCTTCGCGGGGGGGGGGAGCCTCTGTCCTTGTAAGTCAGTTGACTTGCCGC560.16943511542767237No Hit
ATCTAGGGGGATCTATGATGGGTTTATGAAATATGATCTCAGTTCTCTTATG550.16640948836646396No Hit
ACGGGGTAGAACTGTCCGCAAATTCGTCCCGCCTCTGGAGTCTTCAGACCAG540.1633838613052555No Hit
TTTCAAGGGCACGCCATTCTTCTGCCTCAGCCTCCCGAGTAACTGGGACTAC530.16035823424404708No Hit
TAATCCGGGTGTCGGTTCAAGAAGTCATACCCCAGCCCAAGAGAAGCCCTGG530.16035823424404708No Hit
ATTCTCGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA500.15128135306042176No Hit
TCAAATGGGGTACTTAACTTGGTGTTTTGCCTAAAATACAACTAACTCATAA500.15128135306042176No Hit
GGTTAGGGGCTGTAGCATAAGCACCTTCTTAATTAAAGAATGACGCAGTCAT480.1452300989380049No Hit
GTTGCAGGGTTGCGGTGGGTGTCATCAAAGCAGTGGACAAGAAGGCTGCTGG470.14220447187679647No Hit
AATTGTGGGGCTGATCTTCCTGCGGCTGAACCGCCCGGCTGAGCCGACATTG470.14220447187679647No Hit
CTGGCAGGGGTTGCACAAATCTAACTGGTATTTTGTTTTTGTTTTTAGGAGG470.14220447187679647No Hit
GTAATCGGGTACAAAACAGCAGTTATTCTCATAGGACATTCTCTAGGATGGA470.14220447187679647No Hit
AATGCAGGGACCATGCTCCATGTTCATTCGGAGCCAGGAGGGGTTCTCAGTG470.14220447187679647No Hit
TGAAAGGGGAGGTCAGAAGAATGATGGAATGTTTTAGAATAAACTCCTGCTT460.13917884481558804No Hit
GTAATAGGGGACGCCGTACGTGCCAGATGGTGTTACCTGGAGCTTAAAAAGC460.13917884481558804No Hit
CGAATTGGGTCAACCAAGGGGACGCCACAAGCACCCCGTTCATACCTGCGTG450.1361532177543796No Hit
CTGAAAGGGACTCCCTAGTCTTAATGTTTAACCTTGAATGCTATTAACTTAA440.13312759069317115No Hit
AACGTAGGGGCCCACATGGCCTCCAAGGAGTAAGACCCCTGGACCACCAGCC420.1270763365707543No Hit
TATCTGGGGCTGATGTGACCTACCAGATTTCAGAACATGTGTTAATAGTATA410.12405070950954585No Hit
CGCTTAGGGATTGAGAGGAAAGATTATATTGTAGAAAATGAGAAGGAAAAGC410.12405070950954585No Hit
GATTCTGGGAAATCTTTGCTTGATAAGTATTAAGAAATATTGGACTTGCTGC410.12405070950954585No Hit
AGAATGGGGCATTGTTTTCAGAATAAAGAATATTTTTCATAATATTTTAAGA410.12405070950954585No Hit
CATCAGGGGTGACCAGATTGGCTGCTGAAATAGCCCCTAACTTTCTGAAGGC400.12102508244833741No Hit
AGCCAGGGAAGGCTCCATCTCTGCAAGAAAAAAAGAAAAGAAAAGAAAAAGA400.12102508244833741No Hit
GGCACTGGGTAGGGACATGGATGCAATTGGAAATCATCATTCTCAGTAAACT400.12102508244833741No Hit
TTACTGGGGAGTGTATGACAACATCGGGATCCTGGGAAACTTTGAAAAGCAC390.11799945538712897No Hit
TTCGGCGGGCAGTACAGTAACTGAATATGCTAATATGATTTATGTATTTCCT380.11497382832592055No Hit
CTTTCAGGGTCCAGGTGGTAACCACAGAAGGAAGGACGGAACTCACCCCAGC380.11497382832592055No Hit
GCAGCAGGGTAACTTAGTCTTGTTTGACAAAGCTACCTATGATAAACTCTGT350.10589694714229524No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCCTGTCTCTTATAC340.1028713200810868No Hit
AACGCGCGGGTGAGAGACGAAAGAAAAAGAGAAACAATGTGATTGATTTCTC340.1028713200810868No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)