FastQCFastQC Report
Sat 31 Dec 2022
SRR5843964.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843964.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32791
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTGGGAAGCAGTGTGTAGTGGGAAATTTATAGCACTAAATGCCCACAA3060.9331828855478638No Hit
GTTAGCGGGAAGATTCAGGAGTATTGCTATGACAACATTCATTTCATGAAAG3010.9179347991827026No Hit
AAGCATGGGTGTTTGCAATAATGGCCTCCAATACTGAAACAACCAAAAGCAG2720.8294958982647679No Hit
CAAGTGTGGGAAGGATGCAAAGGTCGCTCAAGAGAAAAAGTTGAAAAATACA2670.8142478118996066No Hit
AGCAATGGGCGGTCAGTCCCCACATCAACTGTCTTTTACCCAAGTGATGGCG2440.7441066146198652No Hit
CTTAGTGGGTACAATCAATTAACATTTTATACAAAAGATTGAATTTAAGAGA2440.7441066146198652No Hit
AAAGCTGGGGGAGTGGGGACTTGATCCTGGGGGCAGGGCCTTGGACTTAGGG2430.741056997346833No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGCCGTCT2360.7197096764356073No Hit
CTAGCCTGGGCAACAGAGTAAGACAAAAAAATATATATATATTGAAAATCAA2240.6831142691592206No Hit
GTAATTGGGGTGGCACTGGGCGCGGGCGTGACGGCGCTCAGCCATCCCCTGC2210.6739654173401238No Hit
TGCGCGGGGGCCAAAAGGTAGAAACAATTCAAATGTCCATCAACTGATAAAG2210.6739654173401238No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGC2200.6709158000670915No Hit
TACCTGGGTGAGGTTTTGGGTATGAATGTTCCCATCACCCAAGAACTAAGCA2180.6648165655210271No Hit
AATCAAGGGCACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC2130.6495684791558659No Hit
TCAACCGGGCGATTTCCTAAAGTACCTGGCAAAGAATTCTGCAACTTTGTTC2110.6434692446098015No Hit
ATTGCTGGGTACACTCCAGGCCTCCCATCCTCTGCATCTATACCCCACAGGA2060.6282211582446403No Hit
CAATGTGGGCCTGTTTGCAGCATGCTGTGTGCAAACACTCAGTAATTTGGAG2000.6099234546064469No Hit
GCTTGGAGGGACATATATCAGTGAGAAGTCCGTAAAAACTTACTCTGTTTAT1940.5916257509682535No Hit
AATATGGGATGACAGTTTGACTTGAATGCAACAGCAGGAAAATTTTGCAAGT1900.5794272818761245No Hit
TGCATATGGGACAGTTCTTGGGAAAGAAATGAATGAGAAGTTTGGAATGTCT1880.57332804733006No Hit
TGCCAGGTTTACAAACTCAGACCCTTTTAAAAGTTGTTTAAATTGTTTTGTT1860.5672288127839956No Hit
CGCTAAGGGGAGCGCTGCGGGGCGTCTCCCTCGCAGTCCCGCCCGCCCCTCC1820.5550303436918667No Hit
GTACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1820.5550303436918667No Hit
ACTGTGGGGTATGTTGGAGGAGGGCTTACTGATGCGTGCTAAGACCGATTTC1780.5428318745997377No Hit
AAAAGTGGGAAGGCAGAAATAAAGATGTTCTTTGAAACCACTGAGAACAAAG1740.5306334055076088No Hit
TGTAAAGGGTGTTCCCATGAAGGGAACACAATTACGTTATTATTTTTACAGT1730.5275837882345765No Hit
TCTGCTGGGGTGCAAAAACACCTTTTTGAACAGCAAAGATTTCACCCACCAA1710.5214845536885121No Hit
AGGTGCGGGAACAAAATGTCTTCATACTTTAGGGAAACGAATACACTGTATA1700.5184349364154799No Hit
AGAATTGGGCTTCCCAGACTTTCTCAAGCTCCTCCTCACTGCCCTTCCTCCC1670.5092860845963831No Hit
TAAGTTGGGAGGTGGGAATTGAACAATGAGATCACATGGACACAGGAAGGGG1650.5031868500503187No Hit
TACATGGGGCACCGTTCTGAACTCCCAGATCTGAGAAGGAACTAGTGTTGGT1640.5001372327772865No Hit
CTAACTGGGGGTGGGATGCACACTTGGCCTGGCCTTTCCCAGAGGCAGGAGC1630.49708761550425423No Hit
GCATAAGGGACAACCAAGTGTTGTGTTTTATGTTTTAGGTTTTTATGAAACT1610.49098838095818975No Hit
CACCGGATTTGTGATATCTAAAAATCAAATCAATTGAACTGATTGAACATAG1600.4879387636851575No Hit
GTGCCGGGGCCTAATGGGAGTGCGAGAGCTCTGCACCAGGCACCAGGTTCTC1570.47878991186606085No Hit
TAAGCAGGGCTGACACCCAGTGGATCACCAAACCAGTCCACAAGCACAGGGA1550.47269067731999637No Hit
CAGGCTGGGGTGCAGTGGCGTGATCTTGGCTCACTGCGGCCTCCGCCTCCCA1550.47269067731999637No Hit
AGTCAAGGGTCCTATCAATGCCATATGTGTTAATTCCACCCACATTTTTACT1530.4665914427739319No Hit
TCTTAGGGCAGCTCAACCTCTAAAGCTCATGTTTTTTCTTTCTCTCTGTAAG1530.4665914427739319No Hit
ACTATTGGGTCGGCCGGGGTATGTGGTGATGCCGCCTCTAATTGACATGGGG1510.4604922082278674No Hit
GCTGCGGGGTCTCCTATCACATGCACTCTGAATCAGGGTTTTTCTATAAGAA1490.454392973681803No Hit
ATGTTAGGGTTATCGTAGCAAGGTGCTCTTCTGGGACAACGCGTACGAGTTC1450.44219450458967396No Hit
CTTTATCGGGGTGCTTGGAGTTTAGGAACCCCCAGGATGGCTCTTAGCTGCA1450.44219450458967396No Hit
CATCTGGGGCTCAGGGAGCTGAATATTATGGCAGCCAAGGAAATTGAGGTTG1440.43914488731664175No Hit
GCGCGGGGTGGGCTTCTTGGCACTATAGTCATGGGTATGGACTACGTTAGAA1410.42999603549754506No Hit
TATGCCGGGTGCTACGTCCAGGGTGAGAATAAAGACTTCTCCTCAGGACTCT1400.42694641822451285No Hit
CTAAAGGGGATCCCCATTCCCCCTCTTGGGCCTCAGTTTCCCGACTTGCAAA1390.4238968009514806No Hit
GTGTTTGGGAGGGAATGGGTTCCCTTATTAAGATGTTCTTGCATCCATGTAA1380.42084718367844837No Hit
CCAACAGGGTAAGCCAGGCACCGAATGACAAACTTTGTTTGTTCTCACTTAT1360.41474794913238394No Hit
ATATTAGGGACAGTTTCTTTGGAAATACGTTAAAGATAGTGGCAATTTCATA1350.4116983318593516No Hit
AGCTCCGGGGCTACACTGAGCACCAGGTTGTCGCCTCTGACTTTAACAGCAT1340.40864871458631946No Hit
TTGACGGGGGAGCTCAACTGCTGTGTTTAAAATACACATTTTAAATCCCTCT1340.40864871458631946No Hit
TTGCCTGGGTGCGTATTTCCTCTACACACATCACCCCCCTTCTATAATCTTA1330.4055990973132872No Hit
ATGTAAGGGTGGTAGAACTGAATGATCATTAAATTAGAACAGAAACAGGGGT1320.4025494800402549No Hit
TTGCAGGGATTCACCTGAGATTGCATACATGGCTTTGGAAGCAGGCACTGGA1320.4025494800402549No Hit
ATTCGAGGGAAGGTTTAGACGAGGCACTTCATGGGTTCAAGGAAAGTACATT1270.3873013936750938No Hit
GTTGCAGATCCCTCATGGCTTGGCAGTCCTTGCAATAGTAGGTGAGTTCTCA1260.38425177640206154No Hit
GTTGCCGGCACAGAGAATCCCATCTACAATAGCTACAAAAATTAAAATACTT1250.3812021591290293No Hit
CTCACAAGGGGGATCTGACTCAGGGTGATTGGAGAGATTGAAGAGTGAAGTC1250.3812021591290293No Hit
CTCGGTGGGTATTATTCATGAGTTCTCTATGTAGAATTAGGATTCTGACTAA1250.3812021591290293No Hit
ATGCGAGGGCGATCTGTAGGAGGGTGAAGTGAAGATGTTGGAAAGACAGAGG1240.37815254185599706No Hit
CTTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCCCCTCAGGAACTCTT1220.3720533073099326No Hit
CGACTCGGGGGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGTGAAG1210.36900369003690037No Hit
CAACATGGGAGGGCTTATCAATCTAAGCTCTATTGACATTTTGGGCCTGGTA1130.3446067518526425No Hit
ACCCTTGGGTGAATTCTTTGACTTGTGCTCATTCTTTCTGAACTTATTGAGG1130.3446067518526425No Hit
TTTCAAGGGGAATATGGGAAGCAAGTCTGCCTAACTTCAAGTGGCTTGCACT1120.3415571345796103No Hit
GAACATGGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT1120.3415571345796103No Hit
AAGTGGGTGATGCCCATCTCTTATGAAAGTTATAGAACATAGATGATCAATA1110.338507517306578No Hit
AAACCAGGGCGGCTCACTGCAACCTCCGCCTCCTGGGTTCAGGTGATTCTCC1110.338507517306578No Hit
TAAAAGGGGGGGATTATAGGTATGAGCCACCGTGCCCAGCCAGTAGTTATAA1110.338507517306578No Hit
TATAGAGGGGCCTTTTACATGTTGTCTAATTATCATTTTTCCCCAAATTTTG1090.33240828276051354No Hit
TAACCTGGGTCAGGACTGAAATTTCTGGGCCCCAGGAGATCAAATGCAAAAC1080.32935866548748133No Hit
GACCGAGGGCTTCCGATCTTTGAGTTGGGGGGATTTTGAGTTTACTTGTCCC1080.32935866548748133No Hit
GGTCTAGGGAGGGTTGGGGGTGGGTGGGAAAGTGAGTACTGTGTCATAGTGC1070.32630904821444906No Hit
AATTACGGGGACCCTGTATTAAAGCTTTTGGACGACAATGGGAACATTGCTG1060.32325943094141685No Hit
ACAGTAGGGGGCATGATGACAGGCACCTGTAATCCCAGCTACTCAGGAGGCT1020.31106096184928794No Hit
CTGTTAGGGTCTATCTGCAGAGGTATTCAGTTCCTGGCACAGGGGACTAGGG1000.30496172730322346No Hit
TCTCTTGGGCCCTGGAGGCAGGCTGTACAGAGACATGAGTCAGCTCTCCATC990.30191211003019125No Hit
ATTAGAGGGAGCAGAATGAAAAAATATGTAAACAAATTATCCAATACTTACA980.298862492757159No Hit
AATCCAGGGTGGGAAAGATGCTCAGGGTTCACCATTCTGTTCAGTGGACAAT950.28971364093806223No Hit
CAGTTGGGGTCACTTGTACAGTCCACCTTTTGATACATGCCATGCCTTTGAT940.28666402366503No Hit
CGAATAGGGAGGGATTCTGTGTCTTTGTGTCATAACTTTGATGGGGGCAGTG930.2836144063919978No Hit
TGATGAGGGTGCTTCTAGTAGACCTTAATCTAGCATAGTTGAACCTCTGAAT920.28056478911896554No Hit
ATGAACGGGGGGACAAAGGAGTGGCCGTGAGGTCATCCTCAAGGTCCTGGGG920.28056478911896554No Hit
CTACGTGGGTGAATACAATACTCATCTAGGATGTAACAGTGAAGCTGAGTAA910.27751517184593333No Hit
TGTAACGGGGCATTAAAAAATGGCTTTTCTTTAGGAAAGTATAATCAATTAT910.27751517184593333No Hit
TTTGCCGGGTACAAACTGACAGTAGTTACATTTCAAACCTATCTCCTGCTCT870.2653167027538044No Hit
GCATATAGGGCAGACGAAAGAAGGTAAGGGCCGCTTGGTCTTAAGATATTTG840.25616785093470773No Hit
TGAGAAGGGTGTGCTGGGCAAGTGCTGTGCATTCATAAACCTTCTGTCAATC840.25616785093470773No Hit
TTGTAGGGGTACATGATCAGACACCAGAGGGTTGGGCATTCTTGCAATACCT810.247018999115611No Hit
GAATCCGGGGGGGAGTAGAGAGAAAACAGGCGAGAACAAGCACTCTAATTCC810.247018999115611No Hit
TGAACTGGGAGGGTCTTCAAGACCCTTCAAGACCCATGGCTAATAATTAAGG800.24396938184257874No Hit
GGATCCGGGCGGTTTAATTGGAGTCTTAGAACAGTTGTCTTTGAGAAATTCT790.2409197645695465No Hit
TAATAAGGGCCACCTTAACAATCCTTCCAGCTACGTATCTTAAAGGATGTGA790.2409197645695465No Hit
TTTAAGGGGGGTGGCGATAAAAATGTACCCATAAATTTAAAGTCCATTCTGT780.23787014729651426No Hit
GGGTCAGGGACGGCCGAGCCCTGCAGGCCTCTGCCCTGAAGGCCTGGGGCGG760.2317709127504498No Hit
AGAAACGGATTGGAATAAAGAAAAATATATAGGACTCATAAAGAGCTGAAAT740.22567167820438536No Hit
TGGTTGGGATATAACCTCAGTCCAAAGTGGTGATTGATTTGAGTATTTGAAA730.22262206093135312No Hit
GTTTTTGGGATCCATGTAACGGTGCATCAATATATTGCTATATAAATATGTC710.21652282638528866No Hit
ATTCCTGGGATATGTCTGTGTGCATATAAGTGAAAAGTGGTCAAACAAGAGT690.21042359183922418No Hit
TGAAAGGGGAGGTCAGAAGAATGATGGAATGTTTTAGAATAAACTCCTGCTT680.20737397456619197No Hit
GGTTATGGGGTGACTGACCATGTCTGACTGCCACTCCTTTTTGCAGGAGCAT670.20432435729315973No Hit
AAATCAGGGTATGAAAGATGCTATATTCTATTTCAGCAGGGCCAATATGTGA660.20127474002012746No Hit
TAGTAAGGGGCTTCCACCTCCTTGTAATGAGGTGGGGTAGGAAAGCCTTCAG660.20127474002012746No Hit
ACGGGGTAGAACTGTCCGCAAATTCGTCCCGCCTCTGGAGTCTTCAGACCAG660.20127474002012746No Hit
AGCATCGGGGCACCACGCCCAGCTAATTTTTGTGGTTTTAGTAGAGAAAGGG660.20127474002012746No Hit
CTAGCAGGGGAACGCCCCGATGGTGTGTTACTGACTTTTGGGGGCCAGACTG630.19212588820103077No Hit
CATCAAGGGGCCCTCGGCTTACTTCTCTTCCTTCTCTCCTTAATGACATTAA620.18907627092799853No Hit
GTTGCAGGGTTGCGGTGGGTGTCATCAAAGCAGTGGACAAGAAGGCTGCTGG600.18297703638193408No Hit
GACACTGGGTGCGCCCTGAAGGCAGAGTCCAGGGACCTTGACCCAGCCCCTC600.18297703638193408No Hit
GGTCATGGGCCTGGCTAATTTCTGTATTTTTAGTAGAGACAGGGTTTCACCA580.1768778018358696No Hit
CTCTTCGCGGGGGGGGGGAGCCTCTGTCCTTGTAAGTCAGTTGACTTGCCGC580.1768778018358696No Hit
AGTATTGGGGCATAAAATTAACTGTAGTATGTACTGCACTACTGTAATAATT570.17382818456283736No Hit
TTGTTTGGGCAGCAGCAGCCTGATGGAAGTGATGGGGAGATACAATGTGTTG570.17382818456283736No Hit
TTAACTGGGTCTGTATGTGTTCTGTGTCCCTTGCATGTGTGCGTGTTAGAGT570.17382818456283736No Hit
ACGGTTGGGATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGT550.16772895001677288No Hit
TAATCCGGGTGTCGGTTCAAGAAGTCATACCCCAGCCCAAGAGAAGCCCTGG550.16772895001677288No Hit
AGGTAGGGGTCTACGGGAGGGATTCATATCTGTTTCATTTGGAGGATTTTCT550.16772895001677288No Hit
ATCTTAGGGTGGGTCAGAGAAAACTTCTTGACTAAAACTGGTCAGAACTCAT530.16162971547070842No Hit
CATCTAGGGCAGTACGGGGATTGGCAAGAACGATGCCATCGGCAAAGTCTTT520.15858009819767618No Hit
AGTCGAGGGACCCTGTCTTAATAAGGACTTCTGGGATCACGCTGAGCCGCAG510.15553048092464397No Hit
TTTCAAGGGCACGCCATTCTTCTGCCTCAGCCTCCCGAGTAACTGGGACTAC490.1494312463785795No Hit
AATTGTGGGGCTGATCTTCCTGCGGCTGAACCGCCCGGCTGAGCCGACATTG480.14638162910554725No Hit
TCACTCGGGCTAATAGTATCACTTTGATTTTTCTTTTTCCTCCCTTAGAAAG480.14638162910554725No Hit
TACATTGGGCATATGTGCAGATATTACACTGAAAAAATTGTATCTGTTCCTA480.14638162910554725No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGG460.14028239455948277No Hit
ATCTAGGGGGATCTATGATGGGTTTATGAAATATGATCTCAGTTCTCTTATG460.14028239455948277No Hit
CTGAAAGGGACTCCCTAGTCTTAATGTTTAACCTTGAATGCTATTAACTTAA460.14028239455948277No Hit
ATTCTCGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA450.13723277728645056No Hit
CGCTTAGGGATTGAGAGGAAAGATTATATTGTAGAAAATGAGAAGGAAAAGC450.13723277728645056No Hit
GTAATCGGGTACAAAACAGCAGTTATTCTCATAGGACATTCTCTAGGATGGA450.13723277728645056No Hit
TTCGGCGGGCAGTACAGTAACTGAATATGCTAATATGATTTATGTATTTCCT430.13113354274038608No Hit
GATTCTGGGAAATCTTTGCTTGATAAGTATTAAGAAATATTGGACTTGCTGC390.11893507364825713No Hit
ATAGATGGGTCCAAGTATCTGTCTTTCTTTATGGACCACGGAGGGAAGCCCA390.11893507364825713No Hit
GTAATAGGGGACGCCGTACGTGCCAGATGGTGTTACCTGGAGCTTAAAAAGC370.11283583910219268No Hit
GCAATAGGGTCTTTCCTCCTTTCTCCACTCCAATGGTTTAATGGGATAGAGG370.11283583910219268No Hit
AGATTAGGGGGGACTAAAAGTATTTGACTGGGGCACATGTGGCTGTTGTCAT370.11283583910219268No Hit
CTTTCAGGGTCCAGGTGGTAACCACAGAAGGAAGGACGGAACTCACCCCAGC370.11283583910219268No Hit
GACTTCTTTGAAAGACTTGTCCTGGGCTGACATTGGAGTCTGGGGTGGGCCT350.10673660455612821No Hit
AACGTAGGGGCCCACATGGCCTCCAAGGAGTAAGACCCCTGGACCACCAGCC350.10673660455612821No Hit
CTGGCAGGGGTTGCACAAATCTAACTGGTATTTTGTTTTTGTTTTTAGGAGG350.10673660455612821No Hit
AATGCAGGGACCATGCTCCATGTTCATTCGGAGCCAGGAGGGGTTCTCAGTG350.10673660455612821No Hit
ATATTCGGGTAACTTTAAATGATTAAATGAAACACACAACAGGGAGATCTAT340.10368698728309599No Hit
TATTCGGGGCCTCCCCAGTCCAAAGCTCCTGCCTTTCGGCCACTTCGGCAGG340.10368698728309599No Hit
AACATAGGGTTCGGGAAACCAGAATATGTTTGGGGTGGTAGGAGCCACACAG340.10368698728309599No Hit
TTACTGGGGAGTGTATGACAACATCGGGATCCTGGGAAACTTTGAAAAGCAC330.10063737001006373No Hit
TCAAATGGGGTACTTAACTTGGTGTTTTGCCTAAAATACAACTAACTCATAA330.10063737001006373No Hit
CTCAGTGGGTCCTCTCTTCTCCCTGCTACATTCACTACCAGATTTTTATGCT330.10063737001006373No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)