FastQCFastQC Report
Sat 31 Dec 2022
SRR5843963.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843963.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32694
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGCGGGAAGATTCAGGAGTATTGCTATGACAACATTCATTTCATGAAAG2830.865602251177586No Hit
TGCCCTGGGAAGCAGTGTGTAGTGGGAAATTTATAGCACTAAATGCCCACAA2800.8564262555820639No Hit
CAAGTGTGGGAAGGATGCAAAGGTCGCTCAAGAGAAAAAGTTGAAAAATACA2690.8227809383984829No Hit
AGCAATGGGCGGTCAGTCCCCACATCAACTGTCTTTTACCCAAGTGATGGCG2650.8105462776044535No Hit
TGCGCGGGGGCCAAAAGGTAGAAACAATTCAAATGTCCATCAACTGATAAAG2580.7891356212149018No Hit
CTTAGTGGGTACAATCAATTAACATTTTATACAAAAGATTGAATTTAAGAGA2460.7524316388328133No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGC2430.7432556432372912No Hit
TACCTGGGTGAGGTTTTGGGTATGAATGTTCCCATCACCCAAGAACTAAGCA2430.7432556432372912No Hit
TCAACCGGGCGATTTCCTAAAGTACCTGGCAAAGAATTCTGCAACTTTGTTC2410.7371383128402765No Hit
AAGCATGGGTGTTTGCAATAATGGCCTCCAATACTGAAACAACCAAAAGCAG2300.7034929956566954No Hit
TGCCAGGTTTACAAACTCAGACCCTTTTAAAAGTTGTTTAAATTGTTTTGTT2210.6759650088701291No Hit
AATATGGGATGACAGTTTGACTTGAATGCAACAGCAGGAAAATTTTGCAAGT2160.6606716828775923No Hit
CAATGTGGGCCTGTTTGCAGCATGCTGTGTGCAAACACTCAGTAATTTGGAG2160.6606716828775923No Hit
AATCAAGGGCACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC2140.6545543524805775No Hit
GCTGCGGGGTCTCCTATCACATGCACTCTGAATCAGGGTTTTTCTATAAGAA2030.6209090352969964No Hit
GCTTGGAGGGACATATATCAGTGAGAAGTCCGTAAAAACTTACTCTGTTTAT2010.6147917048999817No Hit
ATTGCTGGGTACACTCCAGGCCTCCCATCCTCTGCATCTATACCCCACAGGA1990.6086743745029669No Hit
TGCATATGGGACAGTTCTTGGGAAAGAAATGAATGAGAAGTTTGGAATGTCT1950.5964397137089374No Hit
AAAGCTGGGGGAGTGGGGACTTGATCCTGGGGGCAGGGCCTTGGACTTAGGG1940.59338104851043No Hit
CAGGCTGGGGTGCAGTGGCGTGATCTTGGCTCACTGCGGCCTCCGCCTCCCA1850.5658530617238637No Hit
CTAGCCTGGGCAACAGAGTAAGACAAAAAAATATATATATATTGAAAATCAA1820.5566770661283416No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGCCGTCT1810.5536184009298342No Hit
CTAACTGGGGGTGGGATGCACACTTGGCCTGGCCTTTCCCAGAGGCAGGAGC1780.5444424053343121No Hit
TAAGCAGGGCTGACACCCAGTGGATCACCAAACCAGTCCACAAGCACAGGGA1730.5291490793417752No Hit
TGTAAAGGGTGTTCCCATGAAGGGAACACAATTACGTTATTATTTTTACAGT1730.5291490793417752No Hit
CACCGGATTTGTGATATCTAAAAATCAAATCAATTGAACTGATTGAACATAG1720.526090414143268No Hit
GTGTTTGGGAGGGAATGGGTTCCCTTATTAAGATGTTCTTGCATCCATGTAA1710.5230317489447605No Hit
AGAATTGGGCTTCCCAGACTTTCTCAAGCTCCTCCTCACTGCCCTTCCTCCC1670.5107970881507311No Hit
TCTGCTGGGGTGCAAAAACACCTTTTTGAACAGCAAAGATTTCACCCACCAA1670.5107970881507311No Hit
AGTCAAGGGTCCTATCAATGCCATATGTGTTAATTCCACCCACATTTTTACT1630.49856242735670153No Hit
AGGTGCGGGAACAAAATGTCTTCATACTTTAGGGAAACGAATACACTGTATA1600.4893864317611794No Hit
GTAATTGGGGTGGCACTGGGCGCGGGCGTGACGGCGCTCAGCCATCCCCTGC1600.4893864317611794No Hit
ACTGTGGGGTATGTTGGAGGAGGGCTTACTGATGCGTGCTAAGACCGATTTC1570.48021043616565734No Hit
GTACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1550.47409310576864255No Hit
AGCTCCGGGGCTACACTGAGCACCAGGTTGTCGCCTCTGACTTTAACAGCAT1540.47103444057013516No Hit
CGCTAAGGGGAGCGCTGCGGGGCGTCTCCCTCGCAGTCCCGCCCGCCCCTCC1540.47103444057013516No Hit
ACTATTGGGTCGGCCGGGGTATGTGGTGATGCCGCCTCTAATTGACATGGGG1520.4649171101731205No Hit
AAAAGTGGGAAGGCAGAAATAAAGATGTTCTTTGAAACCACTGAGAACAAAG1490.4557411145775983No Hit
ATATTAGGGACAGTTTCTTTGGAAATACGTTAAAGATAGTGGCAATTTCATA1480.45268244937909097No Hit
ATGCGAGGGCGATCTGTAGGAGGGTGAAGTGAAGATGTTGGAAAGACAGAGG1470.44962378418058363No Hit
CTTTATCGGGGTGCTTGGAGTTTAGGAACCCCCAGGATGGCTCTTAGCTGCA1470.44962378418058363No Hit
TCTTAGGGCAGCTCAACCTCTAAAGCTCATGTTTTTTCTTTCTCTCTGTAAG1470.44962378418058363No Hit
TACATGGGGCACCGTTCTGAACTCCCAGATCTGAGAAGGAACTAGTGTTGGT1450.4435064537835688No Hit
TATGCCGGGTGCTACGTCCAGGGTGAGAATAAAGACTTCTCCTCAGGACTCT1430.4373891233865541No Hit
ATGTAAGGGTGGTAGAACTGAATGATCATTAAATTAGAACAGAAACAGGGGT1370.41903713219550986No Hit
GTTGCCGGCACAGAGAATCCCATCTACAATAGCTACAAAAATTAAAATACTT1330.4068024714014804No Hit
CTAAAGGGGATCCCCATTCCCCCTCTTGGGCCTCAGTTTCCCGACTTGCAAA1330.4068024714014804No Hit
GCGCGGGGTGGGCTTCTTGGCACTATAGTCATGGGTATGGACTACGTTAGAA1310.4006851410044657No Hit
ATGTTAGGGTTATCGTAGCAAGGTGCTCTTCTGGGACAACGCGTACGAGTTC1300.39762647580595833No Hit
GAACATGGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT1290.3945678106074509No Hit
CATCTGGGGCTCAGGGAGCTGAATATTATGGCAGCCAAGGAAATTGAGGTTG1280.39150914540894355No Hit
GTGCCGGGGCCTAATGGGAGTGCGAGAGCTCTGCACCAGGCACCAGGTTCTC1270.38845048021043616No Hit
GTTGCAGATCCCTCATGGCTTGGCAGTCCTTGCAATAGTAGGTGAGTTCTCA1270.38845048021043616No Hit
TAAGTTGGGAGGTGGGAATTGAACAATGAGATCACATGGACACAGGAAGGGG1250.3823331498134214No Hit
TTGCAGGGATTCACCTGAGATTGCATACATGGCTTTGGAAGCAGGCACTGGA1230.37621581941640664No Hit
TTGACGGGGGAGCTCAACTGCTGTGTTTAAAATACACATTTTAAATCCCTCT1230.37621581941640664No Hit
GCATAAGGGACAACCAAGTGTTGTGTTTTATGTTTTAGGTTTTTATGAAACT1210.37009848901939196No Hit
CTCGGTGGGTATTATTCATGAGTTCTCTATGTAGAATTAGGATTCTGACTAA1210.37009848901939196No Hit
TAAAAGGGGGGGATTATAGGTATGAGCCACCGTGCCCAGCCAGTAGTTATAA1200.36703982382088457No Hit
CTGTTAGGGTCTATCTGCAGAGGTATTCAGTTCCTGGCACAGGGGACTAGGG1180.36092249342386984No Hit
TGATGAGGGTGCTTCTAGTAGACCTTAATCTAGCATAGTTGAACCTCTGAAT1170.35786382822536245No Hit
CGACTCGGGGGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGTGAAG1140.3486878326298403No Hit
TATAGAGGGGCCTTTTACATGTTGTCTAATTATCATTTTTCCCCAAATTTTG1130.345629167431333No Hit
ATTCGAGGGAAGGTTTAGACGAGGCACTTCATGGGTTCAAGGAAAGTACATT1130.345629167431333No Hit
AAGTGGGTGATGCCCATCTCTTATGAAAGTTATAGAACATAGATGATCAATA1110.3395118370343182No Hit
TAACCTGGGTCAGGACTGAAATTTCTGGGCCCCAGGAGATCAAATGCAAAAC1090.33339450663730347No Hit
TTGTAGGGGTACATGATCAGACACCAGAGGGTTGGGCATTCTTGCAATACCT1090.33339450663730347No Hit
CCAACAGGGTAAGCCAGGCACCGAATGACAAACTTTGTTTGTTCTCACTTAT1080.33033584143879613No Hit
CTCACAAGGGGGATCTGACTCAGGGTGATTGGAGAGATTGAAGAGTGAAGTC1080.33033584143879613No Hit
ACAGTAGGGGGCATGATGACAGGCACCTGTAATCCCAGCTACTCAGGAGGCT1080.33033584143879613No Hit
ATGAACGGGGGGACAAAGGAGTGGCCGTGAGGTCATCCTCAAGGTCCTGGGG1080.33033584143879613No Hit
CTTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCCCCTCAGGAACTCTT1050.321159845843274No Hit
ACCCTTGGGTGAATTCTTTGACTTGTGCTCATTCTTTCTGAACTTATTGAGG1040.3181011806447666No Hit
GGGTCAGGGACGGCCGAGCCCTGCAGGCCTCTGCCCTGAAGGCCTGGGGCGG1040.3181011806447666No Hit
GACCGAGGGCTTCCGATCTTTGAGTTGGGGGGATTTTGAGTTTACTTGTCCC1030.3150425154462593No Hit
GGTCTAGGGAGGGTTGGGGGTGGGTGGGAAAGTGAGTACTGTGTCATAGTGC1020.3119838502477519No Hit
TTTCAAGGGGAATATGGGAAGCAAGTCTGCCTAACTTCAAGTGGCTTGCACT1000.30586651985073715No Hit
ATTAGAGGGAGCAGAATGAAAAAATATGTAAACAAATTATCCAATACTTACA1000.30586651985073715No Hit
GTTTTTGGGATCCATGTAACGGTGCATCAATATATTGCTATATAAATATGTC990.30280785465222976No Hit
CTACGTGGGTGAATACAATACTCATCTAGGATGTAACAGTGAAGCTGAGTAA980.2997491894537224No Hit
TTTAAGGGGGGTGGCGATAAAAATGTACCCATAAATTTAAAGTCCATTCTGT980.2997491894537224No Hit
TGTAACGGGGCATTAAAAAATGGCTTTTCTTTAGGAAAGTATAATCAATTAT970.296690524255215No Hit
GAATCCGGGGGGGAGTAGAGAGAAAACAGGCGAGAACAAGCACTCTAATTCC950.2905731938582003No Hit
AATCCAGGGTGGGAAAGATGCTCAGGGTTCACCATTCTGTTCAGTGGACAAT950.2905731938582003No Hit
TTTGCCGGGTACAAACTGACAGTAGTTACATTTCAAACCTATCTCCTGCTCT940.28751452865969296No Hit
AAATCAGGGTATGAAAGATGCTATATTCTATTTCAGCAGGGCCAATATGTGA920.28139719826267817No Hit
TCTCTTGGGCCCTGGAGGCAGGCTGTACAGAGACATGAGTCAGCTCTCCATC920.28139719826267817No Hit
CAACATGGGAGGGCTTATCAATCTAAGCTCTATTGACATTTTGGGCCTGGTA920.28139719826267817No Hit
AAACCAGGGCGGCTCACTGCAACCTCCGCCTCCTGGGTTCAGGTGATTCTCC910.2783385330641708No Hit
CGAATAGGGAGGGATTCTGTGTCTTTGTGTCATAACTTTGATGGGGGCAGTG890.27222120266715605No Hit
TTGCCTGGGTGCGTATTTCCTCTACACACATCACCCCCCTTCTATAATCTTA890.27222120266715605No Hit
TGAGAAGGGTGTGCTGGGCAAGTGCTGTGCATTCATAAACCTTCTGTCAATC890.27222120266715605No Hit
GGATCCGGGCGGTTTAATTGGAGTCTTAGAACAGTTGTCTTTGAGAAATTCT860.263045207071634No Hit
AGAAACGGATTGGAATAAAGAAAAATATATAGGACTCATAAAGAGCTGAAAT850.2599865418731266No Hit
CAGTTGGGGTCACTTGTACAGTCCACCTTTTGATACATGCCATGCCTTTGAT830.2538692114761118No Hit
CTCTTCGCGGGGGGGGGGAGCCTCTGTCCTTGTAAGTCAGTTGACTTGCCGC830.2538692114761118No Hit
AATTACGGGGACCCTGTATTAAAGCTTTTGGACGACAATGGGAACATTGCTG800.2446932158805897No Hit
GCATATAGGGCAGACGAAAGAAGGTAAGGGCCGCTTGGTCTTAAGATATTTG790.24163455068208234No Hit
ATTCCTGGGATATGTCTGTGTGCATATAAGTGAAAAGTGGTCAAACAAGAGT780.23857588548357495No Hit
CTAGCAGGGGAACGCCCCGATGGTGTGTTACTGACTTTTGGGGGCCAGACTG770.23551722028506758No Hit
GGTTATGGGGTGACTGACCATGTCTGACTGCCACTCCTTTTTGCAGGAGCAT750.22939988988805285No Hit
TGAAAGGGGAGGTCAGAAGAATGATGGAATGTTTTAGAATAAACTCCTGCTT730.22328255949103812No Hit
AGGTAGGGGTCTACGGGAGGGATTCATATCTGTTTCATTTGGAGGATTTTCT730.22328255949103812No Hit
AGTATTGGGGCATAAAATTAACTGTAGTATGTACTGCACTACTGTAATAATT720.22022389429253073No Hit
TTGTTTGGGCAGCAGCAGCCTGATGGAAGTGATGGGGAGATACAATGTGTTG720.22022389429253073No Hit
TAATAAGGGCCACCTTAACAATCCTTCCAGCTACGTATCTTAAAGGATGTGA710.2171652290940234No Hit
TGGTTGGGATATAACCTCAGTCCAAAGTGGTGATTGATTTGAGTATTTGAAA680.20798923349850126No Hit
TCAAATGGGGTACTTAACTTGGTGTTTTGCCTAAAATACAACTAACTCATAA680.20798923349850126No Hit
TTAACTGGGTCTGTATGTGTTCTGTGTCCCTTGCATGTGTGCGTGTTAGAGT670.20493056829999387No Hit
TGAACTGGGAGGGTCTTCAAGACCCTTCAAGACCCATGGCTAATAATTAAGG650.19881323790297917No Hit
TCACTCGGGCTAATAGTATCACTTTGATTTTTCTTTTTCCTCCCTTAGAAAG640.19575457270447177No Hit
CATCAAGGGGCCCTCGGCTTACTTCTCTTCCTTCTCTCCTTAATGACATTAA620.18963724230745702No Hit
TAGTAAGGGGCTTCCACCTCCTTGTAATGAGGTGGGGTAGGAAAGCCTTCAG620.18963724230745702No Hit
AGCATCGGGGCACCACGCCCAGCTAATTTTTGTGGTTTTAGTAGAGAAAGGG620.18963724230745702No Hit
CATCTAGGGCAGTACGGGGATTGGCAAGAACGATGCCATCGGCAAAGTCTTT610.18657857710894965No Hit
ATCTTAGGGTGGGTCAGAGAAAACTTCTTGACTAAAACTGGTCAGAACTCAT590.18046124671193492No Hit
GGTCATGGGCCTGGCTAATTTCTGTATTTTTAGTAGAGACAGGGTTTCACCA580.17740258151342755No Hit
ACGGGGTAGAACTGTCCGCAAATTCGTCCCGCCTCTGGAGTCTTCAGACCAG580.17740258151342755No Hit
AGTCGAGGGACCCTGTCTTAATAAGGACTTCTGGGATCACGCTGAGCCGCAG570.17434391631492016No Hit
ACGGTTGGGATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGT570.17434391631492016No Hit
AATTGTGGGGCTGATCTTCCTGCGGCTGAACCGCCCGGCTGAGCCGACATTG560.1712852511164128No Hit
TTACTGGGGAGTGTATGACAACATCGGGATCCTGGGAAACTTTGAAAAGCAC540.16516792071939806No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGG540.16516792071939806No Hit
ATCTAGGGGGATCTATGATGGGTTTATGAAATATGATCTCAGTTCTCTTATG520.1590505903223833No Hit
GACACTGGGTGCGCCCTGAAGGCAGAGTCCAGGGACCTTGACCCAGCCCCTC520.1590505903223833No Hit
TACATTGGGCATATGTGCAGATATTACACTGAAAAAATTGTATCTGTTCCTA520.1590505903223833No Hit
TAATCCGGGTGTCGGTTCAAGAAGTCATACCCCAGCCCAAGAGAAGCCCTGG500.15293325992536858No Hit
CTTTCAGGGTCCAGGTGGTAACCACAGAAGGAAGGACGGAACTCACCCCAGC500.15293325992536858No Hit
TTTCAAGGGCACGCCATTCTTCTGCCTCAGCCTCCCGAGTAACTGGGACTAC490.1498745947268612No Hit
CATCAGGGGTGACCAGATTGGCTGCTGAAATAGCCCCTAACTTTCTGAAGGC480.14681592952835382No Hit
CGCTTAGGGATTGAGAGGAAAGATTATATTGTAGAAAATGAGAAGGAAAAGC470.14375726432984648No Hit
GTTGCAGGGTTGCGGTGGGTGTCATCAAAGCAGTGGACAAGAAGGCTGCTGG450.13763993393283172No Hit
TATCTGGGGCTGATGTGACCTACCAGATTTCAGAACATGTGTTAATAGTATA450.13763993393283172No Hit
CTGGCAGGGGTTGCACAAATCTAACTGGTATTTTGTTTTTGTTTTTAGGAGG440.13458126873432433No Hit
CTCAGTGGGTCCTCTCTTCTCCCTGCTACATTCACTACCAGATTTTTATGCT440.13458126873432433No Hit
TTCGGCGGGCAGTACAGTAACTGAATATGCTAATATGATTTATGTATTTCCT430.131522603535817No Hit
CTGAAAGGGACTCCCTAGTCTTAATGTTTAACCTTGAATGCTATTAACTTAA430.131522603535817No Hit
GTAATCGGGTACAAAACAGCAGTTATTCTCATAGGACATTCTCTAGGATGGA430.131522603535817No Hit
GTAATAGGGGACGCCGTACGTGCCAGATGGTGTTACCTGGAGCTTAAAAAGC420.1284639383373096No Hit
GGTTAGGGGCTGTAGCATAAGCACCTTCTTAATTAAAGAATGACGCAGTCAT420.1284639383373096No Hit
CAATAGGGGTATGCTCTTTGCCTAACAATCTGACAATCCTTTATTCACCAAG410.12540527313880223No Hit
AATGCAGGGACCATGCTCCATGTTCATTCGGAGCCAGGAGGGGTTCTCAGTG390.11928794274178747No Hit
TCTAAGAGGGTGGTGTTATGACTATTTGATAAGTGTACAGTGATTTCTGTTG390.11928794274178747No Hit
TCTTGTGGGCTCGGCACGGGAAGGCTGGGGGTGGTGGCTCACGCCTGTAGTC380.11622927754328012No Hit
CGAATTGGGTCAACCAAGGGGACGCCACAAGCACCCCGTTCATACCTGCGTG360.11011194714626536No Hit
ATACAAGGGTGCAAAGAAAAATGAACTCAAACATAGACTTGCAAGACACAGC360.11011194714626536No Hit
GCAATAGGGTCTTTCCTCCTTTCTCCACTCCAATGGTTTAATGGGATAGAGG360.11011194714626536No Hit
ATTCTCGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA350.10705328194775798No Hit
AACGTAGGGGCCCACATGGCCTCCAAGGAGTAAGACCCCTGGACCACCAGCC350.10705328194775798No Hit
TAATCGGGGGCCCTCTCAGGGAGCAGTCTTCCATCATGCTGAATTTTGTCTT350.10705328194775798No Hit
GATTCTGGGAAATCTTTGCTTGATAAGTATTAAGAAATATTGGACTTGCTGC340.10399461674925063No Hit
CGGTGCGGGAACAAAATGTCTTCATACTTTAGGGAAACGAATACACTGTATA340.10399461674925063No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCCTGTCTCT340.10399461674925063No Hit
AGCCAGGGAAGGCTCCATCTCTGCAAGAAAAAAAGAAAAGAAAAGAAAAAGA330.10093595155074325No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)