FastQCFastQC Report
Sat 31 Dec 2022
SRR5843946.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843946.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132331
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGC4860.3672608836931634No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGCCGTCT4060.30680641724161384No Hit
GGCCGCGGGGAGTACTTTTTACTGTTGCAGAGAATTAAGTCACTAAACGTTA2240.16927250606433866No Hit
TTACAAGGGGTGCAGGATATGCAGGTTTGTTACCTAGGTAAATGTGTGCCAT2080.15718161277402878No Hit
AAGCTGGGGAGTGAGAACATGTGATATCTGTCTTTCCGTGTCTGGCTTAGTT2060.15567025111274002No Hit
TGCAAAGGGGATGAACTTCTTTTGAAAATATGTACATTATTTTTATTCACTT2050.15491457028209565No Hit
CTTGTGGGATTGGAAACAGGACAAAGAATACTATTCAGAATTTTTCCTTGAA2040.1541588894514513No Hit
CTAATAGGGGGAAAAGAGTCAGTCTGCAAAAACCGGAAAAGGAGGTTGTTTT1970.14886912363694071No Hit
TGCGCAGGGATTTAAGCCCAGTTTTCAGGTACTACATCTGTAACTAGTGAAT1960.14811344280629635No Hit
TAAACAGGGTACCTCCAGGAACTCCACCAGGTTCCCACAGTAAATATGAGAG1950.14735776197565195No Hit
GCATGGGGAGACAAGAAAGAAAGACATGGTTTGGGCAATGTATAAATGTCAT1920.14509071948371885No Hit
GCTGCCGGGTGGTTCCTTCTCTTCCTGCGGATGTAGAGGTGGGGCCCCTTCC1850.13980095366920828No Hit
ATCCATGGGTTCTAAAAGCATCACGTCAAGGTTTAAATGAAATGAGATGAAT1830.13828959200791954No Hit
AGCACAGGGACCTTTTCTCTTTTCTTTCTGTCTTGTTAATGCTTTTAAAAAC1820.13753391117727515No Hit
CTGAGCGGGAGGCTGGTCTGTCTGTGGTAATTCTCAAAACAAAATTTCACTT1750.13224414536276458No Hit
AGAGAGGGGAAATGGGAGTGGATGGATGGATGGCAGCCATAAGATCCCAGAT1720.12997710287083147No Hit
GACTGAGGGGGGATAATGGGTCGCAGTGCACTCCCAAGACCTTCGTTGGCAA1710.1292214220401871No Hit
ACCAATGGGGGGACACATTAAAAGTTGAATGGTTGGATGAATGGATGGATGG1710.1292214220401871No Hit
AAAACAGGGGTTGGAGATTCCTTTCCCAGTTTTATAGCACAGTGCCCAGAAT1710.1292214220401871No Hit
ACAAATGGGGATTTATGACGAGCTGCATAACTAAAAAGAAGAAATCCACACC1700.12846574120954274No Hit
GGCAGTGGGAGCGGCCAGCTGCGGGGGCACCTCCAGAACTCCATTCCCTGAG1680.126954379548254No Hit
TTTACTGGGGGCACTAATGAACATTTTGAAAAAAGATGGGAACGATAAGCTT1680.126954379548254No Hit
TACCACGGGGACCTTTGCCTGGGCCATTTTCATGGGATTTAGTATTTGCGGA1680.126954379548254No Hit
ATAAATGGGGGGAGATTGGTGTGATGTTTCTTTGTAAGTGAGGAAGGATTTT1670.12619869871760964No Hit
GTATATGGGATGCAGACAATTGAAATGCAGAAAGTTGAAAAATTATTCAAAT1660.12544301788696527No Hit
TAAACGGGGGGATGGCCGGGTCAAATGGTATTTCTAGTTCTAGATCCCTGAG1640.12393165622567653No Hit
AGTTCTGGGGATTTTTGCAAATACAAAGTATTGTAAATGAATATATGTAATT1640.12393165622567653No Hit
GCCCTCGGGACACTACGTTTTGCTCCAAATCACCAAGATACTGTTTTGGAGC1640.12393165622567653No Hit
ATAGGCCTGGAACATGGTGTATTTCGTTGATTTTCATGTTAAACCACCCTTG1630.12317597539503215No Hit
GCACAAGGGTAAGGCGGTGGGTTGACCACCTTTGTTCTCCACAAGTCAATCA1620.12242029456438779No Hit
TCGCATGGGGTTTATTCACCTTCAGCATCTTTTCATTGAAAATGCGGTAGAT1620.12242029456438779No Hit
AATGGCAGGGCCAGACTCTCAGCTGAAGACAGGGAGCCAATTTCCCCCAGGT1610.12166461373374342No Hit
TTGCACGGGAGATGGTCATGATGTGGCACAACAATGTAAATGTATTTCACAC1610.12166461373374342No Hit
TGAAAAGGGTTGCCAGAATTGTGTTCTGTGTGATTCTGATTACACACCCTAG1610.12166461373374342No Hit
GTTGCAGGGGGCTGTCTGCAATACGAAAAATTCCTGCTTGCCTGCCTGCCTG1610.12166461373374342No Hit
GAAAAAGGGCACTATGAAGTCTAAATCTAAGAGAAGGCTCAGTAATATCGTA1610.12166461373374342No Hit
TGGCGCGGGATTTCCAGGCAGGCTCTCTTACTTGAACACAAATCCAAAAACT1610.12166461373374342No Hit
ATCGGGTCAGATAGTGAGGTTAGCTGAGAAAAGGAAGCATGGTAGAAGAAAT1600.12090893290309904No Hit
ACTAAAGGGCAAGACTTTGATCCTTGGAAACCGTGTGTCCAGCATTGAAGAG1590.12015325207245467No Hit
GCATTAGGGGTCACTAGCTGGGTTGCCCTGCATTTCTGTTGAAAATCAGTGT1590.12015325207245467No Hit
GTAGGCGGGGGAGAAGATTATCGAAGACAAAACTTTCGGTTTGAAGAATAAG1590.12015325207245467No Hit
CAAAAAGGGTTTCACATTTAAGGTTTTCTACTTGGGTGTACTCTAGCCAATT1580.11939757124181032No Hit
GCGAGGGGGAACGATGGAAAACAAAGCGTGTCTTCGATACCAAGTGTCTTCC1580.11939757124181032No Hit
CATAAGGGGGGAGGACAGCAGCGCCGTGCCCCCCGTGCTCCTCTACTCGCTG1580.11939757124181032No Hit
TCTGTTGGGGGTGTATGAAAATATCAGATCTTGGGATCTGCAATAAAAGTGA1570.11864189041116593No Hit
CGCAACGGGTAGACAGGTAGTCATAGGAACAAAGGTAAATGGAGACTTTTTC1570.11864189041116593No Hit
TATCGGGGGAGTCACAGAGATAATACAAATGTGAGGAAATAATGCTGGTGCC1560.11788620958052157No Hit
TCACGGGCGGTATAAAGAATTAAAATTCCCAGGGACAAAAAGGAAGTACAGA1560.11788620958052157No Hit
GCAGTATGCTTGTGGCAATGCCCTTGTCTGTGACAACGTGGAAGATGCCCGC1560.11788620958052157No Hit
TTGTATTGGGGTGAAGTAATCATGATACATGCTGACAACAAAAGATGTAGAT1550.1171305287498772No Hit
AGCTATGGGATAATTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCT1550.1171305287498772No Hit
ACCACAGGGGGAAGCTGCTGCTTGGATTCGCTGGTGGAGGAAAGTGCGACCT1550.1171305287498772No Hit
TTGCGCCGGGCGACGCTCTTCCGATCTTAATAGGGGAAGAAACAAAATGTCT1550.1171305287498772No Hit
GGTAATGGGGAACAAGAAAGTGTGATCTATGCTCAAGAAGCAATTGACAGAA1540.11637484791923285No Hit
GCTTAGGGACCTGATTAAACCTGTCATGAATTATAAAAGGACTTTTTCTTAT1540.11637484791923285No Hit
TACTAGGGGTGAACTCACCAGTGAGTTAGCAGCACGTGTTCCCGACATAACA1540.11637484791923285No Hit
TATTCTGGGTAATATTTTCCCTGTAAAATCTCCTTGGAAAGGCTTTCAGATT1540.11637484791923285No Hit
GACAGAAGGAAGGTATTGAATAAGGTATTCTTGTCAGTTTAGAAATGGACTG1540.11637484791923285No Hit
GCTGATGGGGGATAACAGGCAGCAGAGGACTATAATGCATGAAAGGACAAAA1540.11637484791923285No Hit
CACCTAGGGCTCCTGCCTCTGGATTTTAGGTGTTGATTTCTGGATCACGGGA1540.11637484791923285No Hit
TGACTTGGGGGGAATGCTTTGTGCGGGAAATCCAGAAGTTGCAACAGGAAGA1540.11637484791923285No Hit
ATGTTAGGGGGACCCCAGCCTCTCACTCTCAGTTCCACTCATCCAACTGGGA1530.11561916708858846No Hit
GCCACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1520.1148634862579441No Hit
TGTTCCGGGATTGAAAGATACATGTTCTTAGTTTATGATCCCAAATAATAGT1520.1148634862579441No Hit
GTCCATGGGATTCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATACAA1520.1148634862579441No Hit
CTTCGAGGGGTGGCACCAAGAGTGACCTGCTGGAAGAAGACCGGAGGCGTCG1520.1148634862579441No Hit
TTTAAAGGGGGTGATGATGTTCAAAAAGGGAAGTTTTGAAATTGGAGCCACA1520.1148634862579441No Hit
CAGCACCATTTATTAAATAGGGAATCCTTTCCCCATTTCTTCTTTTTGTCAG1500.11335212459665535No Hit
TTTCTTGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGA1500.11335212459665535No Hit
GACCTTGGGCAACACCAGGTATATCTGATGTTCAGATGAGTTCCAATAAAAA1500.11335212459665535No Hit
ATTGTAGGGCAAACAGTAGATTTCTTCCGGTGTATGGCTTTGTCATTGTTAT1500.11335212459665535No Hit
ATTTGGGGGGACACACCCGGAAGCAGGGGCCCGAGCGGAGCCGGCCGCGATG1500.11335212459665535No Hit
CAAGTTGGGGTGAACACATTGGAACCATAAAATATCAATGCTGGGAGTGTCA1490.112596443766011No Hit
GACGTAGGGGGAATTATTTAGTGTATGAGTGAATGGCTAGTTGAACGCATGA1490.112596443766011No Hit
TGATGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1490.112596443766011No Hit
CGACAGGGGTCTAGTTGTGATGTTACATTGTTAATTTGAGATCTTTCTAACT1490.112596443766011No Hit
AACGTTGGGAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAA1480.11184076293536663No Hit
ATTAGAGGGGAATATAGCCTAGAAAAGACTCGGAGCTTGACTGCTGTTGAAT1480.11184076293536663No Hit
TTGGATGGGGATATTGGCATTGGCACATAAAAACTTTGAAATGTCTATATCT1470.11108508210472225No Hit
CGCTTGGGGTGATATGAAGGGATTAACTGTAGCAGGATAGTCAACCTGACCG1470.11108508210472225No Hit
CAGTTAGGGTCAGATTCCACTTTTGACTTTTCAGATCAGTTTGACACAAGTC1470.11108508210472225No Hit
ATAGGCGGGGTAAAGATAATGCCAAGCCCAAATGGCTTCACTGGTGAATTCT1460.11032940127407788No Hit
TAGGTAGGGCAAAGTCTTGGTGTTAAAGTTGTTTCGTTGTTTAATTTCCTTT1460.11032940127407788No Hit
CACAAAGGGAGTCTACTGTACTGGGTTTAAATCTGAAGATTCCAATTATGAG1460.11032940127407788No Hit
AGGTTTGGGCAGTATTTTGATGTATTGCAAAAATAGATAATAATTTATATAA1460.11032940127407788No Hit
GAAGTCGGGGGACTGAGCCGGGTGGATGGTACTGCTGCATCCGGGTGTCTGG1460.11032940127407788No Hit
TATAGCGGGATATTTTTTAATGTTTACGTCATTTACACTGCTGCTTTTCTCA1450.10957372044343353No Hit
AACAATGGGACATGGATGAAAAGGAGGAGAAGGAATTTAATACTAAGGAAAC1450.10957372044343353No Hit
GCAAGGGGCTCACAGCTGCTATTAACCCTATAATGACTGAAATGACCCCTCC1450.10957372044343353No Hit
AGTAATGGGGATGCAGCAGCTTTGTCATTTCTTCAAAATGTTTAATGTTCCA1450.10957372044343353No Hit
CAGCCATGATCCCAGCAAGCATCACTGTATTGTTTACCTGCTGTGGACTTGA1450.10957372044343353No Hit
GTCGCGGGTAGAAATGAAAACGAAACTGAAGTTCCACAATCTGGGAATAACA1450.10957372044343353No Hit
CAGATAGGGTGATGGGGAAGATGAATTTGTTCCTTCAGATGGGTTAGATAAA1450.10957372044343353No Hit
GCTTTAGGGGGAAAAACTAGCTGGTTTGGTAGAAGAGCTGGAGGCTGACGAG1440.10881803961278913No Hit
TTGTAAGGGAGGAGTGTGAAACAAATTATTTTTATCTTCATCCAAAGATAGA1440.10881803961278913No Hit
ACGACCGGGACTGAGGGAAACAGTTATACTACAAATCATGTTGCAGATCTGA1440.10881803961278913No Hit
ACACGTGGGGAATGCTCATATGTAATTGAAATACTTCAGATCACATGAAAAT1440.10881803961278913No Hit
TTATTGGGGACAAGCAGGCATGCGCAATTCTAATGAAGTCTATCATTTGAGT1430.10806235878214478No Hit
AATACTGGGTTCTGTTTTGTGTAGCCAGGAGGATTTCATAACTTTTCCAAAC1430.10806235878214478No Hit
TGGGCAGGGAATAACAGTCAATGGCATAATAATGCTCTCCTTTTTGAGAAAT1430.10806235878214478No Hit
AGTTAGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1430.10806235878214478No Hit
TACTAAGGGGACTTAGATCCCCCACTACTGTTTTTCTGTGAGAAGCAGCTAT1420.10730667795150041No Hit
TGTCAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1420.10730667795150041No Hit
GTAAATGGGGAAATTGTTTCTATTTTTTAAAAAATAATATTTCTATTCTCAC1410.10655099712085603No Hit
TAGCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1410.10655099712085603No Hit
TGAATGGGGATCCTTCTTTAAATCTCTATTATCAGGACCTGGTGTGTACTGG1410.10655099712085603No Hit
AATGGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1400.10579531629021166No Hit
TTAGCTGGGCACTGAACTCCTGGAGAATGACCCATCAGTAAGGGCAATTCAT1400.10579531629021166No Hit
CAGCCTGGGCAACAGAGCGAGACTCCATCTAAAAAAATAAAAAATAAAAAAT1400.10579531629021166No Hit
ACATTAGGGTGATACGTGTATAGCTATGTAACAAACTTGCAAGTTTTGCCAT1390.10503963545956731No Hit
ACCGCTGGGAAAATATAAATGTTAAAATGTCTCCACTCTAATGTATTTTGCC1390.10503963545956731No Hit
GCACCAGGGTCTCACAAATTCCCACTAAAGAACTTACTCATGTAACCGAATA1390.10503963545956731No Hit
AACAAAGGGATTGCTACAAAGATTTTTTTTCCTAAATGATTCAGTAATTGAA1390.10503963545956731No Hit
TCCACAGGGATGCTTATGGATAGGAAGAATCAATATCATGAAAATGGCCATA1380.10428395462892291No Hit
CTTGATGAGGGGTTGTTAAAAATAAGAGATCTTTGTAGAATAATACTTGGTT1380.10428395462892291No Hit
TGACTCTGGGCTACCTTCACAGAAACATCCAGATGTTTTAAATAAAATATCT1380.10428395462892291No Hit
GAAGCGGGTCTTCAGCAATTACTTCACAACTTAGATTGGTACCAAGAGTGAC1380.10428395462892291No Hit
GCTGCCGGGGATCACCTGGATTTTCAGGACTTCCAGCTTCCTTGGCAACAGG1380.10428395462892291No Hit
ACAACTGGGGCTCTTTCTCAGTGACCGGGTGGTTTGCTTAGGCGCAGACGGG1370.10352827379827856No Hit
ACTGTAGGGACGCCCGGCTAACTTTTTGTATTTTTAGTAGAGACGGAGTTTC1370.10352827379827856No Hit
GCATTTGGGGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGACCCG1370.10352827379827856No Hit
TTTCGGGGGCTCAGCAACTCAGCAGAGTCGCTTCAGTTCACAGCTCTTAATC1360.10277259296763419No Hit
TCGAAAGGGAAGGAACCTACAGACAAATGTGAGTAATCGTCAAAGTTCTTGT1360.10277259296763419No Hit
TCTAAGGGGGGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCAGCAC1360.10277259296763419No Hit
TTAGTCGGGTCTATATACAATGAAATATTATTTAGCAACAAAAAGGAAGGAA1360.10277259296763419No Hit
GCAATGGGATCACTCAGCATTGGATCTAAATATAAAAGTGGTGCTTTCAGTG1350.10201691213698984No Hit
TATCCAGGGGAAAAAGTTTTTGCTGACACAGAACAAACCTGAAAGTAGTATC1350.10201691213698984No Hit
TGTTTTGGGTAATGTGACTATTCTTGGGCGATTTGATTACAGCCAGTGTGAC1350.10201691213698984No Hit
TCGAAGCGGGAAGGGACAGCAAGTGAGCAGAGATGTACTGCAAAGTCGCCAG1350.10201691213698984No Hit
TGCCGGGGTATTACCACTTAGAAGATGTTGTTAAAAACATGTGAAAGATAGG1340.10126123130634544No Hit
GTATATGGGGAAAGATTATGAAAGGAACAGGCTCTTTAAAAAAGAAAACAAA1340.10126123130634544No Hit
AACAAGGGGGAAAAAGAAGGCAGAAAGTACAGAGCAGAGGAGGAAAATAATG1330.10050555047570109No Hit
TGGTCCGGGAGGCGGAGGGGAACCAGCAGCTTTGGACCTTATTGATTGTCTG1330.10050555047570109No Hit
TAGTCAGGGATGATTTGATGTTTAAAAAAGAGGGTGGTTTGAACATAGCCCT1330.10050555047570109No Hit
ACCAAAGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTTC1330.10050555047570109No Hit
TTAGAGTGGGAGAGAATGGAAATGATTACACTACTATATCAGGAAGACTTCC1330.10050555047570109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)