FastQCFastQC Report
Sat 31 Dec 2022
SRR5843945.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843945.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134096
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGC4540.338563417253311No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGCCGTCT4350.32439446366782004No Hit
GCATGGGGAGACAAGAAAGAAAGACATGGTTTGGGCAATGTATAAATGTCAT2530.18867080300680109No Hit
TGCAAAGGGGATGAACTTCTTTTGAAAATATGTACATTATTTTTATTCACTT2000.1491468798472736No Hit
TATTCTGGGTAATATTTTCCCTGTAAAATCTCCTTGGAAAGGCTTTCAGATT1990.14840114544803723No Hit
AGCACAGGGACCTTTTCTCTTTTCTTTCTGTCTTGTTAATGCTTTTAAAAAC1940.1446724734518554No Hit
AAGCTGGGGAGTGAGAACATGTGATATCTGTCTTTCCGTGTCTGGCTTAGTT1920.14318100465338265No Hit
TGCGCAGGGATTTAAGCCCAGTTTTCAGGTACTACATCTGTAACTAGTGAAT1870.1394523326572008No Hit
ATCCATGGGTTCTAAAAGCATCACGTCAAGGTTTAAATGAAATGAGATGAAT1820.13572366066101899No Hit
CTAATAGGGGGAAAAGAGTCAGTCTGCAAAAACCGGAAAAGGAGGTTGTTTT1820.13572366066101899No Hit
ATTGTAGGGCAAACAGTAGATTTCTTCCGGTGTATGGCTTTGTCATTGTTAT1770.1319949886648371No Hit
GGCCGCGGGGAGTACTTTTTACTGTTGCAGAGAATTAAGTCACTAAACGTTA1760.13124925426560075No Hit
TGGCGCGGGATTTCCAGGCAGGCTCTCTTACTTGAACACAAATCCAAAAACT1750.13050351986636438No Hit
AGCTATGGGATAATTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCT1740.12975778546712802No Hit
GTTGCAGGGGGCTGTCTGCAATACGAAAAATTCCTGCTTGCCTGCCTGCCTG1730.12901205106789165No Hit
TAGGTAGGGCAAAGTCTTGGTGTTAAAGTTGTTTCGTTGTTTAATTTCCTTT1710.12752058226941893No Hit
GTATATGGGATGCAGACAATTGAAATGCAGAAAGTTGAAAAATTATTCAAAT1710.12752058226941893No Hit
GACAGAAGGAAGGTATTGAATAAGGTATTCTTGTCAGTTTAGAAATGGACTG1700.12677484787018256No Hit
GCTGATGGGGGATAACAGGCAGCAGAGGACTATAATGCATGAAAGGACAAAA1700.12677484787018256No Hit
GCACAAGGGTAAGGCGGTGGGTTGACCACCTTTGTTCTCCACAAGTCAATCA1650.12304617587400073No Hit
ACTAAAGGGCAAGACTTTGATCCTTGGAAACCGTGTGTCCAGCATTGAAGAG1640.12230044147476435No Hit
CAGTTAGGGTCAGATTCCACTTTTGACTTTTCAGATCAGTTTGACACAAGTC1640.12230044147476435No Hit
AAAACAGGGGTTGGAGATTCCTTTCCCAGTTTTATAGCACAGTGCCCAGAAT1630.12155470707552798No Hit
TAAACAGGGTACCTCCAGGAACTCCACCAGGTTCCCACAGTAAATATGAGAG1620.12080897267629162No Hit
TGCCGGGGTATTACCACTTAGAAGATGTTGTTAAAAACATGTGAAAGATAGG1620.12080897267629162No Hit
ATAGGCGGGGTAAAGATAATGCCAAGCCCAAATGGCTTCACTGGTGAATTCT1620.12080897267629162No Hit
TCGCATGGGGTTTATTCACCTTCAGCATCTTTTCATTGAAAATGCGGTAGAT1620.12080897267629162No Hit
CAGCCTGGGCAACAGAGCGAGACTCCATCTAAAAAAATAAAAAATAAAAAAT1610.12006323827705526No Hit
CGCAACGGGTAGACAGGTAGTCATAGGAACAAAGGTAAATGGAGACTTTTTC1610.12006323827705526No Hit
TATAGCGGGATATTTTTTAATGTTTACGTCATTTACACTGCTGCTTTTCTCA1600.11931750387781886No Hit
TAGCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1600.11931750387781886No Hit
CTAAAGGGGATGTTTGAATATGTGCAGTTTTCAACATTTTGGCACATGAAAG1580.11782603507934614No Hit
TACCACGGGGACCTTTGCCTGGGCCATTTTCATGGGATTTAGTATTTGCGGA1580.11782603507934614No Hit
GAAAAAGGGCACTATGAAGTCTAAATCTAAGAGAAGGCTCAGTAATATCGTA1570.11708030068010977No Hit
TTAGTCGGGTCTATATACAATGAAATATTATTTAGCAACAAAAAGGAAGGAA1570.11708030068010977No Hit
AGGTTAGGGAAGCAGCAGGAAGAAGTATTTCGAAATTTAGATGCTCAGTATG1570.11708030068010977No Hit
AACGTTGGGAAACTAACTAATACTAACATCTCAGACGCTCAGGAAATAGAAA1560.1163345662808734No Hit
TTGCGCCGGGCGACGCTCTTCCGATCTTAATAGGGGAAGAAACAAAATGTCT1560.1163345662808734No Hit
TTTCTTGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGA1560.1163345662808734No Hit
TGACTTGGGGGGAATGCTTTGTGCGGGAAATCCAGAAGTTGCAACAGGAAGA1550.11558883188163703No Hit
AACAATGGGACATGGATGAAAAGGAGGAGAAGGAATTTAATACTAAGGAAAC1540.11484309748240067No Hit
TTGCACGGGAGATGGTCATGATGTGGCACAACAATGTAAATGTATTTCACAC1540.11484309748240067No Hit
TTGTAAGGGAGGAGTGTGAAACAAATTATTTTTATCTTCATCCAAAGATAGA1540.11484309748240067No Hit
ATCGTCGGGGGCATTTAGATGGCTACTTTGAAACCCTTTTGTAAATGAAATT1540.11484309748240067No Hit
CATAAGGGGGGAGGACAGCAGCGCCGTGCCCCCCGTGCTCCTCTACTCGCTG1540.11484309748240067No Hit
ATAGGCCTGGAACATGGTGTATTTCGTTGATTTTCATGTTAAACCACCCTTG1540.11484309748240067No Hit
ATTTGGGGGGACACACCCGGAAGCAGGGGCCCGAGCGGAGCCGGCCGCGATG1540.11484309748240067No Hit
AGAGAGGGGAAATGGGAGTGGATGGATGGATGGCAGCCATAAGATCCCAGAT1530.1140973630831643No Hit
CTTGATGAGGGGTTGTTAAAAATAAGAGATCTTTGTAGAATAATACTTGGTT1530.1140973630831643No Hit
TGTCAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1530.1140973630831643No Hit
ATAAATGGGGGGAGATTGGTGTGATGTTTCTTTGTAAGTGAGGAAGGATTTT1530.1140973630831643No Hit
ACAAATGGGGATTTATGACGAGCTGCATAACTAAAAAGAAGAAATCCACACC1520.11335162868392794No Hit
GCAATGGGATCACTCAGCATTGGATCTAAATATAAAAGTGGTGCTTTCAGTG1510.11260589428469156No Hit
CAATAAGGGATTCCTGTGCTGTGTTTTTTATTACCCTTGGTAGGTATTAGAC1510.11260589428469156No Hit
CGACAGGGGTCTAGTTGTGATGTTACATTGTTAATTTGAGATCTTTCTAACT1510.11260589428469156No Hit
TCTGTTGGGGGTGTATGAAAATATCAGATCTTGGGATCTGCAATAAAAGTGA1510.11260589428469156No Hit
CAAGTTGGGGTGAACACATTGGAACCATAAAATATCAATGCTGGGAGTGTCA1500.1118601598854552No Hit
ATCGGGTCAGATAGTGAGGTTAGCTGAGAAAAGGAAGCATGGTAGAAGAAAT1500.1118601598854552No Hit
CGCTTGGGGTGATATGAAGGGATTAACTGTAGCAGGATAGTCAACCTGACCG1500.1118601598854552No Hit
TTGGATGGGGATATTGGCATTGGCACATAAAAACTTTGAAATGTCTATATCT1490.11111442548621883No Hit
TATCCAGGGGAAAAAGTTTTTGCTGACACAGAACAAACCTGAAAGTAGTATC1490.11111442548621883No Hit
TTTCGGGGGCTCAGCAACTCAGCAGAGTCGCTTCAGTTCACAGCTCTTAATC1490.11111442548621883No Hit
GTCAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1480.11036869108698247No Hit
GATGCATTGATTAGATTTGTTCTTTATGTTGCCAGGCCCAGAAGAACCCAGC1480.11036869108698247No Hit
ACCAATGGGGGGACACATTAAAAGTTGAATGGTTGGATGAATGGATGGATGG1480.11036869108698247No Hit
GCACCAGGGTCTCACAAATTCCCACTAAAGAACTTACTCATGTAACCGAATA1480.11036869108698247No Hit
TTAGAGGGAGGCCTTTATGATCAACCATTTCTCTAACCCCTTTCGCTAAATT1480.11036869108698247No Hit
AGTTCTGGGGATTTTTGCAAATACAAAGTATTGTAAATGAATATATGTAATT1480.11036869108698247No Hit
GAAGTCGGGGGACTGAGCCGGGTGGATGGTACTGCTGCATCCGGGTGTCTGG1480.11036869108698247No Hit
ACAACTGGGGCTCTTTCTCAGTGACCGGGTGGTTTGCTTAGGCGCAGACGGG1470.1096229566877461No Hit
CAGCCATGATCCCAGCAAGCATCACTGTATTGTTTACCTGCTGTGGACTTGA1470.1096229566877461No Hit
GACCTTGGGCAACACCAGGTATATCTGATGTTCAGATGAGTTCCAATAAAAA1470.1096229566877461No Hit
TCACGGGCGGTATAAAGAATTAAAATTCCCAGGGACAAAAAGGAAGTACAGA1470.1096229566877461No Hit
GACTGAGGGGGGATAATGGGTCGCAGTGCACTCCCAAGACCTTCGTTGGCAA1460.10887722228850973No Hit
TGTTCCGGGATTGAAAGATACATGTTCTTAGTTTATGATCCCAAATAATAGT1460.10887722228850973No Hit
CTTGTGGGATTGGAAACAGGACAAAGAATACTATTCAGAATTTTTCCTTGAA1460.10887722228850973No Hit
CACCTAGGGCTCCTGCCTCTGGATTTTAGGTGTTGATTTCTGGATCACGGGA1460.10887722228850973No Hit
AATGGCAGGGCCAGACTCTCAGCTGAAGACAGGGAGCCAATTTCCCCCAGGT1450.10813148788927336No Hit
TTATTGGGGACAAGCAGGCATGCGCAATTCTAATGAAGTCTATCATTTGAGT1450.10813148788927336No Hit
ATACGTGGGCTGATTGCTGGCAGGAAATGTTGGGAATTTGGTTTTATGGTCT1450.10813148788927336No Hit
ATGGTAGGGAGTAAAAATCTGGACAAGGACATGTATGGTGATGACCTAGAAG1450.10813148788927336No Hit
TCTAAGGGGGGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCAGCAC1450.10813148788927336No Hit
GCAGTATGCTTGTGGCAATGCCCTTGTCTGTGACAACGTGGAAGATGCCCGC1450.10813148788927336No Hit
CACATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1440.107385753490037No Hit
GGTGAGGGGATCTAATCGAGGAGCATTCAGGACACAATCAGATGTCTAGCTT1440.107385753490037No Hit
AGTAATGGGGATGCAGCAGCTTTGTCATTTCTTCAAAATGTTTAATGTTCCA1440.107385753490037No Hit
GGAGCTCAGATGGACTCGGGAGGGAGAAACAGGAAGAGCCCTCTATGGCCCA1440.107385753490037No Hit
TACTAGGGGTGAACTCACCAGTGAGTTAGCAGCACGTGTTCCCGACATAACA1440.107385753490037No Hit
GCTGCCGGGTGGTTCCTTCTCTTCCTGCGGATGTAGAGGTGGGGCCCCTTCC1440.107385753490037No Hit
CTGAGCGGGAGGCTGGTCTGTCTGTGGTAATTCTCAAAACAAAATTTCACTT1440.107385753490037No Hit
CTCCCTGGGCATCTATGTGGGAAAGCATTCACTGAATCTTCTGTGCTTAAAC1440.107385753490037No Hit
TGAATGGGGATCCTTCTTTAAATCTCTATTATCAGGACCTGGTGTGTACTGG1440.107385753490037No Hit
TGCCGGGGTATGGTAGCTCTATTTAGTAACTGTCCTGTTGCTTACCAAATCC1430.1066400190908006No Hit
GCTAAGGGGGAGAAGTCCACCTTTTAGTTGCAGAATCCACGCTTATATTGAG1420.10589428469156424No Hit
GACAAAGGGACTGTTCACAATGGCCAGGATGTGGAGTCGGCGAGGGTGTCCA1420.10589428469156424No Hit
TATAACGGGCGATCTACACGTGGGCAGGGCAGGGCTTTAGGATGTTCTGGAT1420.10589428469156424No Hit
TAAGCTGGGCTACAGTAAGACTCCGTCTCAAAAACAAAACAAAACAAAAAAA1410.10514855029232788No Hit
GGCAGTGGGAGCGGCCAGCTGCGGGGGCACCTCCAGAACTCCATTCCCTGAG1410.10514855029232788No Hit
GTAAATGGGGAAATTGTTTCTATTTTTTAAAAAATAATATTTCTATTCTCAC1410.10514855029232788No Hit
TTCTTAGGGCTAAAATGGAAATGTCAATAGAGGAGCATGGTCCTATGTGAGG1400.10440281589309151No Hit
TTAGTAGGGCATGTCAGTATTAGAAGAATAGGCAGCAGTTCCTTAGTTTACA1400.10440281589309151No Hit
AACGCTGGGAAGACCATACCAAAAAGATAAATTTAAAAATTATTACACATTT1400.10440281589309151No Hit
CTCTGTGAGGGACAGTGGGGAGGGCTGTGAAGGCTCATGTGACCTGGATCTG1400.10440281589309151No Hit
AATAGTGGGGAGGAATTTTTTGTTGTTTTGTCTTGTTTTGTTTTTGTTTTCA1390.10365708149385515No Hit
CTGCGAGGGTTAATTATTTGGTGGAAATGTTTACACTTTTTGCTTTGCATTT1390.10365708149385515No Hit
GCTTAGGGACCTGATTAAACCTGTCATGAATTATAAAAGGACTTTTTCTTAT1390.10365708149385515No Hit
GAAGCGGGTCTTCAGCAATTACTTCACAACTTAGATTGGTACCAAGAGTGAC1390.10365708149385515No Hit
CAACGTGGGCTCCTATAAGTACAGAATACATTTTTCTCTTGGCATATTTTGA1390.10365708149385515No Hit
CAGCCCGGGATGGTCATCTCCTTGTAACTTAGCTTAAATTAAAGTACCTTTA1390.10365708149385515No Hit
GTAGGCGGGGGAGAAGATTATCGAAGACAAAACTTTCGGTTTGAAGAATAAG1390.10365708149385515No Hit
GCAAGGGGCTCACAGCTGCTATTAACCCTATAATGACTGAAATGACCCCTCC1380.10291134709461877No Hit
GCACTTGGGGTCCAGGTGGTGAGGTATAAGTTACTTGCTCTTAGCATTTGGT1380.10291134709461877No Hit
TTTTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1380.10291134709461877No Hit
TAAGCTGGCGGATCAGAAAGCTTAAGAACTCAGACTTAATCCCAGAGATAGG1380.10291134709461877No Hit
TCGAAGCGGGAAGGGACAGCAAGTGAGCAGAGATGTACTGCAAAGTCGCCAG1380.10291134709461877No Hit
GCGCCGGGATAAATTCCGATGATAAAAAATAGTGTTCCACTCCCTCCCTTGG1370.10216561269538241No Hit
CAGCGGGGGTTTCTGACTGACATTATTTTTGTCTCATAGGGTGGCACCTGTG1370.10216561269538241No Hit
TATACAGGGTCAGTACTTGACATTCCTCCACGGAAGGGAAGACAAGTATCAA1370.10216561269538241No Hit
TTACAAGGGGTGCAGGATATGCAGGTTTGTTACCTAGGTAAATGTGTGCCAT1370.10216561269538241No Hit
TTTACTGGGGGCACTAATGAACATTTTGAAAAAAGATGGGAACGATAAGCTT1370.10216561269538241No Hit
TAGGCTGGGCTCCCCTTTTTAAAAACTAGTATACGGCTACATTTCTTTTAGA1370.10216561269538241No Hit
TGATGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1370.10216561269538241No Hit
ACCACAGGGGGAAGCTGCTGCTTGGATTCGCTGGTGGAGGAAAGTGCGACCT1370.10216561269538241No Hit
TTTAAAGGGGGTGATGATGTTCAAAAAGGGAAGTTTTGAAATTGGAGCCACA1370.10216561269538241No Hit
TGTATCGGGCTCATTCATTGCTGGTGGAAATACAAAATGGCACAGCCACTGT1360.10141987829614604No Hit
GCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATA1360.10141987829614604No Hit
AACTAAGGGGAGTGTTAATCATTTTTAAATACTGGCCAATAGAACAGCCAAA1360.10141987829614604No Hit
ACCTAGGGGTGAGCTCCCAGATTCGTCAGAATTATTCCACCGACGTGGAGGC1360.10141987829614604No Hit
TTGACCGGGGTTTTAGATGATAACCACAGGTCTACAAGAGCGTTTTTAGTAA1360.10141987829614604No Hit
ACGTGAGGGACAGCAGATTTTGCTGGTTACTACAAGCTTGAAAGATGGGTCT1360.10141987829614604No Hit
AGGTATGGGTGTGCGTGTGTGTGTGTGTAATTTTTTTGGTTTCCTAGTAATC1350.10067414389690968No Hit
GACTAAGGGCATATTAAACATTTCTGTTTGTACTTTTATTTATTAGGTGCTA1350.10067414389690968No Hit
ACATTAGGGTGATACGTGTATAGCTATGTAACAAACTTGCAAGTTTTGCCAT1350.10067414389690968No Hit
TAGTCAGGGATGATTTGATGTTTAAAAAAGAGGGTGGTTTGAACATAGCCCT1350.10067414389690968No Hit
ATTAGAGGGGAATATAGCCTAGAAAAGACTCGGAGCTTGACTGCTGTTGAAT1350.10067414389690968No Hit
GTTCTCGGGTCGATAGAAATAAGGTTAAACAAGTTCAAGAATCAACTAACAT1350.10067414389690968No Hit
AATACTGGGTTCTGTTTTGTGTAGCCAGGAGGATTTCATAACTTTTCCAAAC1350.10067414389690968No Hit
TCGAAAGGGATCCGTAGAGTAGTCATGACACTATACAGATGGTTCGTGTTCA1350.10067414389690968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)