FastQCFastQC Report
Sat 31 Dec 2022
SRR5843936.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843936.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170443
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGCCGTCT9200.5397698937474698No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGC8270.48520619796647557No Hit
AATTTGGGGGTATGCAGGACAGATTTTAGAAACTTAGATTAAAATACAAATC2140.12555517093691146No Hit
TAGCAGGGGGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.11968810687443897No Hit
TGTATAGGGGTGCCATTCATGGCTGATCTGGAATTCCATTTCTGCCTTTGCT2020.11851469406194445No Hit
CTATATGGGACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.1179279876556972No Hit
GTGTTGGGGACAGAAAGAATGAATGGACACATGAACGACTGAATTAGAAATG1980.11616786843695547No Hit
CTTTCGGGGTAAAATATTCATTTGGAATGAGTTTAAAAGGCTGATTTAGAAA1970.1155811620307082No Hit
ACTGTGGGGATTATTGTGTCTTTGTCAAACAACAAAAACTTGAACTTAGCCC1960.11499445562446096No Hit
ATTGCTGGGACACTTTTTCTGTACTTACTAGGTTAAGGCAGATTTATTTTTA1950.11440774921821371No Hit
ATCCAGGGGTAAGTAAGGAGAGTCTGCTGGAGATGGAGTTTAGTGAGAAAAA1950.11440774921821371No Hit
TATACAGGGAAGTAAAATAATAATAATAAAAATGGTTTATGAACCAAAAGAC1920.11264762999947195No Hit
TTTGTTGGGGTGCTGGAAATAATGTGAAAGTGTGGGAAAAATACTTCCAAAA1920.11264762999947195No Hit
AATCACGGGCAACCTGAAGTAGAAGTGTTTCACAGAGAATGCTAATTTCTGG1910.11206092359322471No Hit
TTTAGTGGGATTCCATGAGAACTTCTGATAGAGAAAGAGTTGTGCCACTTAG1890.11088751078073023No Hit
GTGACTGGGGATTGTAAATATGGAGCTGTTCTGATGTCTGCTTTTGACTTTT1870.10971409796823571No Hit
ATTTTTGGGGAACAAATTCAGAGGATCTTAGGTGCTGGCTTGTGGAGACAAA1860.10912739156198846No Hit
ATCAATGGGCTGACCACAATAGAATGAAATTAGAGTCAATAATAGAAAGGTA1840.10795397874949397No Hit
TCATCGGGGGAGGTGTTGGCATTCTTCGCTGATTTGGCTGTTCCCAATGTTT1830.10736727234324671No Hit
ATTGCTGGATGGCGCCATTGCACTCCAGCCAGGGCAGCAAGACCAAAACTCC1820.10678056593699946No Hit
AACCCAGGGGAAAAACTAATGTTAAAATATCCATTTCACCTAAAGCAATGTA1820.10678056593699946No Hit
ACTACGAGGGGAAGTTAGCACTATGTTTACATGCAAAAGATTAAGGAAAAAA1820.10678056593699946No Hit
CGAAGCGGGAATCCCACTGTGGATATTTACCCAGAGGAAAGAAAATCAATAT1810.10619385953075222No Hit
AGCTGTGGGGATGGAGGTTTTAAAAACACAAACAAAGAAAAGAGGCAGTTGA1810.10619385953075222No Hit
TTAATGGGGACCTGTTTTTTTGTATATTACATTTAAGTTTTCATTTACCACA1800.10560715312450497No Hit
GTGCATGGGCAGTGGTCTGGATACGAAGTTTACAGTTTCATACCAACTATCT1800.10560715312450497No Hit
TATTGAGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1790.10502044671825772No Hit
TCATTAGGGGTAGTTCCCCTTGAAGAGATACCTCACATCCCTTGTTAGCTGT1770.1038470339057632No Hit
CAATTCGGGGGTAGAAAAGAAATTAGTAAAGAAACCTGAGAAAGAGTAGCCA1770.1038470339057632No Hit
GTTGCAGGGCTAGAAGTAAAGTCACCCAGTGTTCCCAGAACACCAAAAGAAG1760.10326032749951597No Hit
TGCAATGGGTAACAAGAAATGTGTAGATAGTAGCTCCAGTACTTGAAGCATC1750.10267362109326872No Hit
CATACTGGGATGTTACATGAATTCTACTTCAATCAAGCTGGTGATTTAAAAA1740.10208691468702148No Hit
ACATTAGGGAAGAAGAAACGTTTCCTAACAAAATAAAACAGTTTATTGAAAA1740.10208691468702148No Hit
GTGACTGGGGCTTCAGGAGTGCCTCACGTAGATAGATTGAGGTTTTATAATA1730.1015002082807742No Hit
ATGAACGGGGAGAGAAGAGAGGCGCCTGCGCTTGCGAGCTGGGCTTGTGAGT1720.10091350187452697No Hit
TGTCAAGGGATGTGGAAAAGCCTTTAACCAGTTCTCAAATCTTACTAAACAT1710.10032679546827972No Hit
TGCGCTGGGTACACTGGTCATGAAAGTCATTAACATTAGGGAAATTGGGTAA1710.10032679546827972No Hit
TTCTCACGGGTGGTTACACATATGGAAGTAAGCAAAATGAAAAGCACTTATT1710.10032679546827972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)