FastQCFastQC Report
Sat 31 Dec 2022
SRR5843933.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843933.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89753
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGCCGTCT4650.5180885318596593No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTTGAAGGATCTCGTATGC3120.34762069234454557No Hit
CGCTAGGGTGGACTAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGA1520.169353670629394No Hit
GAACAAGGGCTCCAGGGGCAGCTTTGAGCAGAAGCTGAACTGGGCCTTCAAT1440.16044031954363644No Hit
TAAACAGGGAGAGACAGCTTTGGGTATCACCATCAAACAAGTATCTGAAGAA1410.1570978128864773No Hit
ATGAAAGGGGAGTGAAGTAACTTCCACACTGTCAAGAGTTGACAGCATATTA1350.15041279957215914No Hit
TATCCTGGGGACAATAAACTGGAAAATCTAGAAGAAATGGAAAAATTCCGAG1280.14261361737212128No Hit
GTTACAGGGAGTTCCCTAATTGTTGAATAATTAGCTGAACGTACAAATCTGA1250.13927111071496218No Hit
ACATTTGGGGCACTTGGCTAGGAATAATAATATTTTAAAACAACAACAACAA1250.13927111071496218No Hit
TTTTGTGGGAAGACTCGTCATGAAGAATTTAAAAAATATGAAATGATGAAGG1230.13704277294352277No Hit
GTAGGAGGGGAGACCACTGATTGTACTATAGAAATGATTTCTTGTAGGGATG1230.13704277294352277No Hit
GGTTGCGGGAAGATAGATTTGTCTGGGGAAGAAGAATCACAATGCCACCAAT1220.13592860405780308No Hit
TGCATAGGGGGCAGAGAGAAGTGGTCAAAATCTGGATATATTTTGGAGGTTT1170.1303577596292046No Hit
GTTCATGGGCAAATCTATTTTTCTCACGTGTTTAAAAAACTGAAAAGTGACT1150.12812942185776519No Hit
GATACAGGGGGAAACAGAAGTTCTGGGAGAAAACCCATGCAGACAGCAGGAG1150.12812942185776519No Hit
ATATTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1140.12701525297204552No Hit
CTTCAGGGATACTAAGGAACTGAGTAGAATTATTTGGGAATGCTGAGTACCT1130.1259010840863258No Hit
CTTTCTCAGGGTACCGTGGCACACTTTCTGGTCACTTTGTACAATGTAGATT1130.1259010840863258No Hit
AAATCGGGAGGCGTGAACCACTGCGCCCGGCCAGCATGCATTTTAAAAGTGG1120.12478691520060611No Hit
CGTTAAGGGATTATCACTTCTTCATATCCGATAATATTAAAGTTCCACAGAA1120.12478691520060611No Hit
TTTCAAGGGTAACCAAGGTTTCAGGTATCCTCCTGTCACCACCTCTACTATT1120.12478691520060611No Hit
CATTTCGGGTACGAGGCTGCTGTTATTTGTATATGATTCTTAAAAATCAAGC1120.12478691520060611No Hit
TTTTAAGGGATGCATGAATTTTGGTACATGTGGTAGACCCGAAACCAATCCC1120.12478691520060611No Hit
ATAGTTCGGGACCTTAGGAAAGAATGTTTACTGAATGTTTATTTTATTTTTT1120.12478691520060611No Hit
AACCATGGGACTCAACATCATACAGATGTCAGCTCTTCTCAAGTTAACATGT1110.12367274631488641No Hit
TAACCGGGTGTCAGATAACATAGCAAATAATGGAACATATCATCCTTGATAT1110.12367274631488641No Hit
TCTTTGGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1100.12255857742916672No Hit
ATGGCTGGGACTCCAAGTCTCCTAATTTATTCATGCGGAGATGCATCTGCTA1090.12144440854344701No Hit
TCAAGCAGGGTCCACATTTTTGAAAGGATTTAGTATGAATTTTCTAATTTTA1090.12144440854344701No Hit
AGCGCTGGGGAAGACAGTTTGGATGTTTCCTACAAAACTAAACACTTTTACC1090.12144440854344701No Hit
CTTTGAAGGGATTTCTCTATAGTTATTTTAAAATAGTAATCAATGCTATGTA1090.12144440854344701No Hit
AGTACAGGGAAGGTGAACCAAAAGATCTATGACAACATGTGAGAAAGTGGCA1090.12144440854344701No Hit
GCAGCAGGGTAATAAAAGGTGTTCATTGACACCCCATTACTGGTGGTTGGAC1090.12144440854344701No Hit
CTCATTGGGGCCTGATGTTAACTTACATCCCTTGAACATTCTCAGCTAGACT1090.12144440854344701No Hit
TCTAATGGGAGTCTAATGCTCAGTTAGCGTTAATACTGAGAGAAGTATTTCG1070.11921607077200762No Hit
TCTAGAGGGACAACCAGTCTTTTTAAAGGGCAAAAATGACTCAACACCTTTG1070.11921607077200762No Hit
CCAGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1070.11921607077200762No Hit
TGCTCAGGGATAATTAAGACTTTTGTGAAGTATTTTGTGAGCTCTCTCTGGG1060.11810190188628793No Hit
GATGCAGGGTGCTGACAAGTTGGATGTGGGGTGTGAAAGAAAAATAGGGACT1060.11810190188628793No Hit
CTACTTGGGGAAGCTACAGATTGTCATAGTTGTTTTCCTGCTTTACAAAATT1060.11810190188628793No Hit
ACTTCAGGGCAGGTGCCCGCGACCCGCGTGTCCCCGTGCGCCTGGCCGCCTT1060.11810190188628793No Hit
ACTTTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1050.11698773300056822No Hit
TAGCAGGGGTGCCAATTATATGTCTGGGTTTCTCTCCTTATTAGCTACAGGC1050.11698773300056822No Hit
ACAAATGGGACAATAAAACAATGCTATTTATCTATCAGAATGTCTTAAACTG1050.11698773300056822No Hit
TTAGGGGGTTGAACTCCTGGGATCAAGCGACCCTCCTCCCTTGGCCTCCCAA1040.11587356411484853No Hit
ATTGCAGGGCGATCTTATCTAGGGACCTATGATACATCTGACCTGGCTGCTA1040.11587356411484853No Hit
TGTTTGGGGGAAAATTACTGAATCTGTCATGTGTTTTTAGTTGAAGTGAGAG1040.11587356411484853No Hit
GCGCTGGGAAGGTATTTGTTGCAACTTACTACATAGCATATACCCAAAATCA1040.11587356411484853No Hit
TTCAGCGGGGCAGACTGCTGCGGTTCCTGGGTGGCGGCGGCATGGAGGCGCG1040.11587356411484853No Hit
ATCACTGGGTACAGGAGTTAAAGTACATAAATGCCTTTTTACAGCTTAATCA1040.11587356411484853No Hit
TCGTTGAGGGATGTTTTAGTTCTAAGGAAGCACCACTCAGTACAATGAAACA1040.11587356411484853No Hit
CCATCTGGGAAGTGAGAAGCGCCTCTGCCCGGCCATCTCATCTGGGAGGTGT1030.11475939522912883No Hit
TGAAATGGGAGGAGCTACTGAGGGAAATCAGTGTCATTATTTAAAGTCACGC1030.11475939522912883No Hit
GAATATGGGGTAATCCGTCAAATTCTTGTATAACTTTGAATAAATGGTTAAT1030.11475939522912883No Hit
GCACTAGGGCAAAATCTCTTTCATATTTCCATACGGTCCATCAGGCATTTTT1030.11475939522912883No Hit
TCAACGGGGTTATGTTTGAGAGTTGAAGCCTACTGCCAGGGTCTCACAGTGA1030.11475939522912883No Hit
GCTTACGGGAGGAATGAGAGGCAAAAATTCTAAAGATTTGGGTTATATTTTC1030.11475939522912883No Hit
AGAATAGGGTCCAAAAGAAATGAAATCACTATATCCAACAGATTTTTGCCCG1030.11475939522912883No Hit
CTCATTGGGATCCAAGACTACATAGAGAAGGCAGTAAATGGCAGGGTTAGTC1020.11364522634340914No Hit
GAGGCAGGAGAATGGCATGAACCTGAGAGGCGGAGCTTGCAGTGAGCCGAGA1020.11364522634340914No Hit
CAACGGGAGAGGGAGCTGTTCAGCAAGCTGGGGGAGCTGGCTGTGGGCATGA1010.11253105745768943No Hit
AATCTGGGGCAAGAAATAGGTTATAGGTAAAATCTCTGAAGGATCATCTATG1010.11253105745768943No Hit
ATTTAAGGGACCATGATAAGTCAGAATACTGGAGGCAAAAAGAAGACTCTGT1010.11253105745768943No Hit
GTCAGGGGACTTAAGAGTAGAACTTACACTTATTTTTTACCATTATCTTCTC1010.11253105745768943No Hit
GTATGAGGGATGGAAAAGTACAAAAGAAGTAACTCAAGAAGAAATTAAAAAT1000.11141688857196974No Hit
TTAAGCAGGGAGGCTGGCTCTGTGGGACACTGTCAACAATGGGCCAGCCCGG1000.11141688857196974No Hit
TCCTATGGGTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCCGC1000.11141688857196974No Hit
GTTATAGGGACAGTCCCTTGTTCAACAGAGAGCTTTTGCTGGGAAGACGGCA1000.11141688857196974No Hit
AGCGATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA990.11030271968625004No Hit
CTACACAGGGTGGGATTGCAGGAACATGCCACCACTCCTGGCTGATTTTTGT990.11030271968625004No Hit
CATTTCGGGTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGATTATGTC990.11030271968625004No Hit
TAGCTTGGGTCCGATCTAGACGAGGGCAACCAACAAGTCATTATTACCCTCA990.11030271968625004No Hit
ATTGTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA990.11030271968625004No Hit
CGTACTGGGATCTTCTCCACAAATTATTATAACTCTGCAAGTTTGGGCAAGT990.11030271968625004No Hit
TGTGCAGGGCACCGACATAGTCATTATGCCGAAGAGAAAGTCTCCAGAGAAT990.11030271968625004No Hit
CATATTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA990.11030271968625004No Hit
GTGGTTGGGACTCGAAAAGAACAAAGGAGCTCTTTGTGCATCAGGTTCTCAA980.10918855080053036No Hit
ATCATTGGGGAATTCTCTTCCTTAAGAAAGCTGTATAGTATTACGGGAAATA980.10918855080053036No Hit
TGCTTTGGGCCTCGGAGGAGAGTGGAGAGAAAGTATGTTTCAAGAAGCAATG980.10918855080053036No Hit
CTCTACGGGATTATAGATAAATTAATCATTGTTGATTTTATCTGTCACATGT980.10918855080053036No Hit
TAAGCTGGGAAGTTGAATCAACATATGTTCTTTCATAGATGTTAAAAGGTTA980.10918855080053036No Hit
TTTTATGGGATATAAATTACACCAGAGCTTCATATTAATGATACTAACTGGC970.10807438191481064No Hit
GAGCAAGGGGTTGTCACTTAAGTTGCCACCTCTGCATAAGAGCTCTCTGATC970.10807438191481064No Hit
CTTACAGAGGGGACCAGCACCATGGCGGTTGGCAAGAACAAGCGCCTTACGA970.10807438191481064No Hit
TACGTGCGGGCTTCTGCTACTACTACTTAGTGCTTTGCTGTGTATAGGGTGT970.10807438191481064No Hit
CACAGCGGGGCAACCAGGGAATTTAATTATTGCAGAAACAAGTTTAATAAGG960.10696021302909096No Hit
ATATCCGGGAACTAAAACTAACTGAGCAATCAAGTTTTATTTTTCTCAGATA960.10696021302909096No Hit
TTTTTCGGGAGAAATTCAGTGACGAAGGTGCGTTGTAGGGGTATTGATGTGC950.10584604414337125No Hit
TCTCCAGGGATTTCATGTAGAATTAATTGCTAGTTATGATGCTTGTGGATAG950.10584604414337125No Hit
AATGCTGGGATCACCTGGGAGATGAAGGAAGCAGAAGGGAGAGGAAAGTAGG950.10584604414337125No Hit
GGCGCAGGGAGCATAAGTTAGCAATCGGTTCTTCCAAACTCCTGTGGGTTGA950.10584604414337125No Hit
ACTGATGGGACCTTCATTTAAGAGGCAGAAGATTAAGATAAGAAATATAAGA950.10584604414337125No Hit
ACCAGCGGGGGGAAATGCCTGAAGTACTTTGGCGATGTCCAGGTATTTCACA950.10584604414337125No Hit
TACGTTGGGGCAGTGGCGGCACCTGGGAATCTGAATTTGTCATGCTTTCGAG950.10584604414337125No Hit
AGTACAGGGGTTGTTCTGAGATGATTTTAATATACACTACAGTGTTTTTGTA950.10584604414337125No Hit
CCCCAGCTTAAATGTACTGGAACGTTTGTCAGTGAGTTTCATTTCCAAAGTT940.10473187525765157No Hit
TCACTGTGGGGGTTGGGAATTTGCCTGGTCAGATAATGTGAATTCTAGAGTC940.10473187525765157No Hit
TCAAAAGGGATCCGAAAGAAACAGAAAGAAAGAAAGAAAAGAAAGAAAGAAA940.10473187525765157No Hit
TAACCAGGTAATGAAATGAGATGAAATAATGAAATGATGAAATAATGAAATG940.10473187525765157No Hit
TTGCATGGGGTATTCCTGAAACACAATTTAACATCAAAACACCTTAGTCTGA940.10473187525765157No Hit
GCCACTGGGGGTTTTTTCAGAGTTTGGCTAAGAATGTTGTTACCATCTTCTT940.10473187525765157No Hit
TAGCAGGGTGACCTTACCAGCCAACCAAAGAGGGGCAGGACATTTGGAAAAT930.10361770637193185No Hit
AGTAGGGGTCATTTGATGTTTCAAATAGCAAAGATGCGGGAGCCACTGAGCC930.10361770637193185No Hit
GTTGTTGGGGACTCATCCAAATCTCTCTAAAACTTTCCAATGAAATCAGCCA930.10361770637193185No Hit
ATTGGTGGGATCTTCACTGCCAAGCAAAAAGTCTGCCAAGTGGGAATGTTCA930.10361770637193185No Hit
TCATCAGGGGTTTTCTAAGAGCCCTCAATTGCCTGCCTGTATTAATTTTAGT930.10361770637193185No Hit
CCAGCTTAGTGGCCAGTGTGGCTTACTGTTCTCTCTAACTTAGAGATGATGA920.10250353748621217No Hit
GCGCAGAGGGACTGCTCTCCAAACTTCTATTTTATTGATTTATGCTCTAATT920.10250353748621217No Hit
GAGTGGGCCAACAATGGCAACTGTGTTGTCATAAGACCAAAAAATCTTCAAT920.10250353748621217No Hit
GTATGGGGAAATTGATTGGTTCTTAATTAGTTGTTTATTGTTCTTCTAATTC920.10250353748621217No Hit
AACGACGGGGATTTTAGAAGCCATTATTTCTTATCCATTCTGATCTTGTTGT910.10138936860049245No Hit
GCCATGGGAGATTAATGCGGAAGACAACCATCTACATGAGTGGTATGGAGAG910.10138936860049245No Hit
TGAACTGGGAGCAGCTCCAGATGTCCTTATGGCATAAGAAATGGAAAAGTGC910.10138936860049245No Hit
AGCTGTGGGATAGTGAGGAATATTCAAAATCTGGAAAGATATTCTATTTTTT910.10138936860049245No Hit
CACCTGGGGAATTTTGGGGTGTGACACTTTTGAGCGGTTGAATTGGGAGAAT910.10138936860049245No Hit
AGCTGCGGGAAGAGTCAGTTGAAAGAAAGACAAGAGAGTCACATCTTAGCTT910.10138936860049245No Hit
TCACATACGGGATCCTGCTGTGCAATAGAACAAGAACTTATTTCTCCTATCT910.10138936860049245No Hit
TACTCAGGGCCGATCTATGAAAGGGTGCAGGCTTTTCTGAATACCAAATCTG900.10027519971477278No Hit
TTGCTGGGGCTGAGTAATTCTCTAACTGGAACTAAAGTGCGTTTTAGAAATT900.10027519971477278No Hit
TATCGCGGGATCCCATATTGATTTTAGGAATTGACTCTAAAACTCTGTGCAG900.10027519971477278No Hit
ACCTCAGGGAAACAGAAGAAATCATTTTTTTGCTATGTAATACCTCGCAACT900.10027519971477278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)