FastQCFastQC Report
Sat 31 Dec 2022
SRR5843878.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843878.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146572
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGC3660.24970662882405917No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGCCGTCT3410.23265016510656877No Hit
GTTAAGGGTGACAGCGTCTTTTAATTAATGTGTTTTGGACCATTTACATTTA1800.12280653876593074No Hit
AGAAATGGGGTGATTAAGAAATCAGAATTTATAGATATTGGGATAAATGAAG1780.1214420216685315No Hit
GGATTTGGGCAGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGCAATTCTCC1770.1207597631198319No Hit
TAGTACGGGAGTATATATATTTCAAGCTGAATGTTCAGAAAGAATAAGGTGA1740.11871298747373304No Hit
CGTATCGGGAAGATCAGCAACATCATCAAACAGTTCAAGCTGAGCTGCAGTA1680.11461943618153536No Hit
GATGCAGGGGTGATGGGGGCGTGCGGCAGGATCCAAGGCACAAGTGGAGGAG1670.11393717763283574No Hit
TGCAGGGGATCTTGGTATGGGATCCCCAGGAAATGTCCACATACACACAAGA1650.1125726605354365No Hit
TCTATAGGGATATTTGGCTTAGAATAAATCTCTTCAAATATATTTTTTTAAA1620.11052588488933766No Hit
CATTCAGGGACGTTTATCTATGTAACAAACATGCTGCACATGTACACCAGAA1600.10916136779193844No Hit
TCTCATGGGTAACATTCTTAGTTATTTTAATTTTTTTTGTGAACTTGACATT1580.1077968506945392No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1580.1077968506945392No Hit
AACACTGGCAATATGGCAGCTTTAAATTCTGAGTTACTACAGTCTCCATGTC1540.10506781649974074No Hit
ACCCGACCTCACACTGCAGTTTCAGGCACCGCCCGGGCCCCCAAATCCAGGC1530.10438555795104112No Hit
TTCTCAGGGAATGTTCAAATCTTTCCTAAAGATCCTTCAACTTTTCTGGAAG1520.1037032994023415No Hit
TGACTAGGGCAGTGGAGGGCATCCACACCTCCATTTTATGCTTGTGGTTCAC1510.1030210408536419No Hit
CGTGCTGGGTGACCTGAATCACTACCATGTAAAAGTGTCATACTTGTGATTT1500.10233878230494228No Hit
GTTGCAGGGTACCAGAGAAGATCACTTTCACAAAAAGAATATAGGACAGAAG1500.10233878230494228No Hit
GACAAAGGGCCTAGAAGACTTATATAATGGTGCAACAAGAAAACTGGCTCTG1480.10097426520754305No Hit
TGTGTTGGGGTAGCAATCAGAGCACACAGCAAATGTCACCATTGGCATAGCC1480.10097426520754305No Hit
GAATATGGGTGGATCAAACAGAACTAGAGAAAAGTTTAAAAAGGCACGTGAC1470.10029200665884344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)