FastQCFastQC Report
Sat 31 Dec 2022
SRR5843877.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843877.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146363
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGC3650.24937996624830047No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGCCGTCT3260.22273388766286562No Hit
GTTAAGGGTGACAGCGTCTTTTAATTAATGTGTTTTGGACCATTTACATTTA2010.13732978963262574No Hit
GGATTTGGGCAGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGCAATTCTCC1860.12708129786899694No Hit
GAATATGGGTGGATCAAACAGAACTAGAGAAAAGTTTAAAAAGGCACGTGAC1760.12024897002657776No Hit
AGAAATGGGGTGATTAAGAAATCAGAATTTATAGATATTGGGATAAATGAAG1740.11888250445809391No Hit
CGTGCTGGGTGACCTGAATCACTACCATGTAAAAGTGTCATACTTGTGATTT1730.11819927167385201No Hit
GGTAACGGGCAGCCCCAGTTCTCAGGTTTTCATCTGCCTGGGTCTGATCCTG1720.11751603888961008No Hit
TAGTACGGGAGTATATATATTTCAAGCTGAATGTTCAGAAAGAATAAGGTGA1700.11614957332112623No Hit
CATTCAGGGACGTTTATCTATGTAACAAACATGCTGCACATGTACACCAGAA1680.1147831077526424No Hit
GATGCAGGGGTGATGGGGGCGTGCGGCAGGATCCAAGGCACAAGTGGAGGAG1660.11341664218415856No Hit
AACACTGGCAATATGGCAGCTTTAAATTCTGAGTTACTACAGTCTCCATGTC1630.11136694383143281No Hit
TGACATGGGATTTATGACTGCCGTAGATCTAATGGAAGCAAGCCAACTGTGT1620.11068371104719088No Hit
AGCTTTGGGGCCGGGCTACGTCGTGCCCTGCGCGTGAGCAGCTGCAGCGGCA1610.11000047826294898No Hit
TGGGTTGGGGGCAATGCTCTGCTGGTTTATTTTCAAGTGGCTGCGTTTTTTT1600.10931724547870705No Hit
TTACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1600.10931724547870705No Hit
ATCTGGGGGGTTTCTGCCATTTGCCCCAGTAATCCTCCACTGCCAAGTAGCA1590.10863401269446513No Hit
TTCTCAGGGAATGTTCAAATCTTTCCTAAAGATCCTTCAACTTTTCTGGAAG1580.1079507799102232No Hit
CGTATCGGGAAGATCAGCAACATCATCAAACAGTTCAAGCTGAGCTGCAGTA1560.10658431434173937No Hit
AGGCTGGGGCGACGCTGCGGAGATGGAGCGCTGTCTGGCGCGCAGGAGCGGA1530.10453461598901362No Hit
CTAACCGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1530.10453461598901362No Hit
CGTATAGGGAGAGCGTGCAGACGGAGGATGGCACTGGCGGGATGGCCAGGGC1530.10453461598901362No Hit
GTTGCAGGGTACCAGAGAAGATCACTTTCACAAAAAGAATATAGGACAGAAG1530.10453461598901362No Hit
TACCTGGGGGGGAGCCCGTTCGGTTGCGGGTGTCTCTGGCCCTGCGGTCAGC1520.1038513832047717No Hit
AATGTAGGGAGATTTACATCTTGAAAGATGTATTTTTCCAAAAGATGCCACT1520.1038513832047717No Hit
CTTACTGGGAAATCAGTTAGATGGTAAGAAAAAGAAACCAACTTCACCAGTA1510.10316815042052978No Hit
ATGATAGGGACTTGGTATAATGCTATTGAATTTTTCACCTCCAGGCATGATT1510.10316815042052978No Hit
TCGACAGGGCTTTCATGTACATTATAATACACACAAATACATACGTGTATAT1500.10248491763628785No Hit
AAGTTTGGGAATGTACTGAATGTGGCAAGGTCTTCAGTCGCAATTCACACCT1490.10180168485204595No Hit
GATTATGGGAAGATCTCAAAGGGCTTTGAGTAGGATTGTCCCATGATCAGAT1480.10111845206780402No Hit
TCTCATGGGTAACATTCTTAGTTATTTTAATTTTTTTTGTGAACTTGACATT1470.1004352192835621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)