FastQCFastQC Report
Sat 31 Dec 2022
SRR5843876.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843876.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147394
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGC3990.27070301369119504No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGCCGTCT3310.22456816424006404No Hit
GTTAAGGGTGACAGCGTCTTTTAATTAATGTGTTTTGGACCATTTACATTTA2110.14315372403218585No Hit
CGTATAGGGAGAGCGTGCAGACGGAGGATGGCACTGGCGGGATGGCCAGGGC1910.12958465066420616No Hit
TTACCGGGGATAATTCCTAAGGGGGAAATTAATAAACTTCATAAAAATTAAA1770.12008629930662035No Hit
TCTATAGGGATATTTGGCTTAGAATAAATCTCTTCAAATATATTTTTTTAAA1720.11669403096462541No Hit
GGCAATGGGCATATTAAACTCTTAAGTCAAATTAAGCAGACCCGGCGTTGGC1700.11533712362782746No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1670.1133017626226305No Hit
GGATTTGGGCAGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGCAATTCTCC1650.11194485528583253No Hit
GTTGCAGGGTACCAGAGAAGATCACTTTCACAAAAAGAATATAGGACAGAAG1610.1092310406122366No Hit
TGACATGGGATTTATGACTGCCGTAGATCTAATGGAAGCAAGCCAACTGTGT1600.10855258694383761No Hit
GATGCAGGGGTGATGGGGGCGTGCGGCAGGATCCAAGGCACAAGTGGAGGAG1590.10787413327543861No Hit
CGTGCTGGGTGACCTGAATCACTACCATGTAAAAGTGTCATACTTGTGATTT1570.10651722593864066No Hit
TGCGTGGGTGCAAAAATCCTAAACAAAATGCTAGCAAACCAATTCCAACAGC1570.10651722593864066No Hit
ATAACAGGGTCATGGTGTTTGAATTATGTGGCAAATCAGTTGTTAAAAACCC1550.10516031860184268No Hit
CAGATAGGGATATACATTTTTTGTGATCTTTATTTCCAGTGATGAGCACTGC1530.1038034112650447No Hit
GACAAAGGGCCTAGAAGACTTATATAATGGTGCAACAAGAAAACTGGCTCTG1520.10312495759664572No Hit
GAATATGGGTGGATCAAACAGAACTAGAGAAAAGTTTAAAAAGGCACGTGAC1510.10244650392824675No Hit
CATAAAGGGAGATAGAGGAGCTGAATAAAGAAGGTGATGAAATCTAAGTACA1510.10244650392824675No Hit
CTTTGCGGGACAGAAAAAAAAGGTTCTGGGATCTACATGGCAGGGAGGGCTG1480.1004111429230498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)