FastQCFastQC Report
Sat 31 Dec 2022
SRR5843872.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843872.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93815
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGC5780.6161061663913021No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGCCGTCT5710.6086446730267014No Hit
TTGCTTGGGATCCAGGTGAAAGAACTGGAGAAGCGTGCCTCAGGCCAGGCTT1930.20572403133827213No Hit
GAGCTTGGGACCAACACCATCATCACCACCATCACCATCACAACCATCACCA1860.1982625379736716No Hit
AGACGTGGGTGCAAGCCTCATGATAAACTCAAATCCAAAAATATACAATGCA1610.17161434738581252No Hit
TGACAGGGGGAAAATTGAAGGCTAGTTAAAAAGACCAATATTATTAAATTTG1600.17054841976229812No Hit
GCCATTGGGGGGAGCAGGCGCTGCGGCAGGAGGGAAGATGGCGGACGAGGAG1490.15882321590364015No Hit
GCAGCTGGGCTGAGCTCTTTGAGGAATGGACAGGTAATGTGAAGACAATGGC1480.15775728828012578No Hit
AATAACGGGATGACAAAAAAGAAACATGAACTGTGAGATGCAATATAAAAAC1420.1513617225390396No Hit
AGTTCGGGGCAGTCAATTATAATGCTGCTTACCTCTTCTCTGTTGAAATAAT1390.1481639396684965No Hit
AAGCTTGGGTAAGATTATTTCAGGTCAGTTTCTTTCTGATAAGAAAGTTGGG1390.1481639396684965No Hit
AATCATGGGTATAACGACTAATCTAAATGTGATACATATTTAGGCACCTTCT1370.14603208442146778No Hit
TAAACTCGGGACAAAACAATTACTAATAGACCTAAGAAATGAGGTGGAGAGC1370.14603208442146778No Hit
GCTTTGGGGATTCTTGGCATACTTAGCAAGGTGTGTAGGAGTACAAGAGCCT1350.14390022917443906No Hit
TCTGAAGGGATGGTAGACAATGAGGCCATCTATGACATCTGTCGTAGAAACC1340.1428343015509247No Hit
GCAAATGGGAGTCTGCAGCAAGACATTCCTATATTGAAGAAATGAGAGAAAA1330.14176837392741032No Hit
CTCTATGGGAACGCAGCCACTGAGGAAGAGTGGTTTTCCTAAGAAGACATTG1320.14070244630389597No Hit
ACTCAGGGGGGGTCCCTCCCACAACACATGGGAATTCAAGATGAGATCTGAG1300.13857059105686725No Hit
CGCTTTGGGGTACCTTAAAAACATACCTACCCTAATTTCCAGTCAAGTTTTT1290.13750466343335288No Hit
CAAACTGGGGTAGACATATTACTTAGGAAGACAATTTTGTAAACAAGATTTA1280.1364387358098385No Hit
TTTGCTGGGGGGAGGGGGACTCAAATTTCAGAAAAGCTACCAAGTCTTGAGT1280.1364387358098385No Hit
GTCCACGGGGCAGGGTCTTTGCTATACAAAAGTGTTTTCCACTAATTTTGCA1270.13537280818632413No Hit
TACCTGGGGGTCTTTGTTTAGGTTTCTGTTTTAGCAACTGAAATTTTAACGC1270.13537280818632413No Hit
AGGACAGGGGGCCTAATTTTAAAGGTCCAAAATAACTTGTTTTTAAAGTTTG1260.1343068805628098No Hit
TTGTCAGGGCAGCTCACTGCATCCTCTGCCTCCCGGGTTCAAGCAATTCTCT1250.13324095293929542No Hit
TTAACAGGGAGTCAACTAATGCGACTTATGGTAGCCAAGGAAGCCCGCAATG1230.1311090976922667No Hit
TCTTCAGGGATACTCCTAGGTGAAAAAAAAGATGGAGACAAGGGAAAAAATG1230.1311090976922667No Hit
TTTAAGGGGATATATGATTGTTTGAATTAAGATGCAAATAAGGTCCATACAT1230.1311090976922667No Hit
TGCGCTGGGGATCTAAATCGGGGGGGTTCGCACACTCCTCCATTATGGCATT1230.1311090976922667No Hit
GGATACGGTGGGTAAATTTCTAAGTGGAAGTTTTAAAAATTAAGCATCAGTA1220.13004317006875232No Hit
AGTTGCGGCTACTTTAGCAATAATTAATTCCATGATTATCACATTCTGCCAT1220.13004317006875232No Hit
GATGTTGGGCGGCCTGGCTTCCTGACCTTCTGGGACCAGTGCACAGAGAGAC1210.12897724244523798No Hit
TCACCTGGGGTATGTGTTTGTATATGCTTCTGTGACAAAAGAAAAGAAAGTA1200.1279113148217236No Hit
ATCGATGGGTGGGTGTTTTCAGGGCTTGTTTTGAGTTTTGCTGAATAGGGAG1200.1279113148217236No Hit
CATCACGGGTACTTTCTAAACAGTAACTGAAAATACTTAGAGTTAAACTTGC1200.1279113148217236No Hit
CTTATGGGGCCATCCTGTCTCGCCGTATTGCTGTTGAATATAGTGATTCGGA1190.12684538719820923No Hit
TTCCTTGGGATATTGAAGAACTGTAGGAGAAATACAAAAGAAGTAACATATG1180.12577945957469489No Hit
GCACTAGGGATTTGTATGATATTTTAAAATCTCCTAAATGCTGGGCAATGGC1180.12577945957469489No Hit
TTTAATGGGCCCATAAAAATCCCGATGGTGGAAGGGTGGAACAAGAGAACTT1170.12471353195118051No Hit
ACCACGGGGGGTAATATAGATCATTTCTTTAGTCTGGTATAGGTCTAATTTT1160.12364760432766615No Hit
GAAGCTGGGGGTTCCGGCTACTCAGGAGGCTGAGGTGGAGGGATGGCTTGAT1160.12364760432766615No Hit
ACAACAGGGCCATCTCCTACCTCCACAGTACAGACTGTCCCCAACTTAACAG1160.12364760432766615No Hit
ATTTGCGGGTATAAAAAGTGCATTGTGTGGCTAATTCACACAGGTTATATTG1160.12364760432766615No Hit
CTGGATGGGACACCACGAACAGAAGTAATGAAATCCCTTCCACAGACGTCAC1150.12258167670415178No Hit
AGCGGAGGTTGCGGTGAGCTGGGGTTGCGCCATTGTACTCCAGCCTGGGCAA1150.12258167670415178No Hit
ATCGTAGGGGTAAGTTTTATTTATACTCTTTGGACTCCCATGAACTTTTGCA1140.12151574908063742No Hit
ATAGATGGGAGAGATGAGTTGTGTGGGCAGTGAGTTGAGTATCACATTGCAT1140.12151574908063742No Hit
ACTCAGGGTCCTGTTTCGTTAAAAGTGAAACATTTTTTAAAAGGAAAAATAA1130.12044982145712306No Hit
ACATGGGGGAGTGTCAAGTCTTGTGAATTTACTCCATGGGTTGTTTGCCATC1120.1193838938336087No Hit
GACTAAGGGGAATATTCTTTCCTTCAGGGGAAAGAAGTATAATCTTGATTAT1120.1193838938336087No Hit
ACGTGCGGGGAAAATGCTTAAGTAAAGATAATACACAATAATCTAGTTATAT1120.1193838938336087No Hit
TAACAGGGAGTAAAGAACATGCTCAGTGAGTGAGTGGAGAAGAATTTTAGTT1120.1193838938336087No Hit
CACTACGGGAGGAGGCTGAGGCAAGAGAATTGCTTGAACACAGAAGGCAAAG1120.1193838938336087No Hit
ATACTAGGGGACCATATTATCTATAAGGCTTTGGTCAACAGTAAGACAGTAG1110.11831796621009434No Hit
TGAAGCGGGCTCTTGCAGAAGCTTCAACTTGGCAGACAGATCGTGAAGAGAC1110.11831796621009434No Hit
TTCTCAGGGATGCTGAGGGGATTCTGGGCAGTGCATTTCAACATCCAGAAGA1110.11831796621009434No Hit
TTTATGGGGACGCTCTTCCGATCTGCACTTGGGCTTTTTCCTTCAGCAGCAT1100.11725203858657997No Hit
TGGCATGGGATTGGCTCCGAGACGAGAGCCATCTACTCCATCGTGCTCAAGC1100.11725203858657997No Hit
TAGCATGGGAAGGGGGAAAGTCTCTTACTGAAGTGCCTGAAACCCAAAGCTA1100.11725203858657997No Hit
AACTAAGGGAGTTTCAGACCTCAGCAGCTGTAAGGCCAGTCCAGGGAACCCT1100.11725203858657997No Hit
ACATGTGGGTGGCTTCTGCCTGGCTCAGGAGACCTGGATTCTGGTCCCTGCT1100.11725203858657997No Hit
CAGCCTGGGTGACACAGCAGGACTCTGTCTCCAAAAGAAAAGGGAGGAATTT1090.11618611096306561No Hit
ACACTGGCCCCTCGCACTCCAGAAAAGCCATGCCAGCTGGGCTCGTTGACAA1090.11618611096306561No Hit
AGACAGGGGAACCTTTCTAATGAAGGTATACATATTATTCAAGAGTGACTAA1090.11618611096306561No Hit
TATTAGGGTGGGTTTTTTGCTATTGAATTGTTTGAGTTCCTTATATATTCTG1090.11618611096306561No Hit
ATGCGAGGAGATAACACACGTTTGGAAGCCATACAGTCTGTAGACGCAATAC1090.11618611096306561No Hit
CACTAGGGGACTCCAGGCCTGCAAGACCTCTCCTCCCCAAACTTTCTACAAC1080.11512018333955125No Hit
TACTGAGGGTACCTATTGGAAATCATAGTAAATCTAGACTTCTCTACTCAGC1080.11512018333955125No Hit
GGGTTAGGGATAATCAGACGCATATGTTGCGAAAGAGTGAAGGGACTTGGAC1080.11512018333955125No Hit
GTAAATGGGAACATGTGGCAAGTTCTATATGAATATTCTTGGTCATCTCGAC1080.11512018333955125No Hit
CTAACGAGGGAAATCCAAATGACTATCCCAAAGACATAGGAATATACGAATA1080.11512018333955125No Hit
TTTTCAGGGCAAAGTAAATTAACATAAATGCTAAATTTAGGTTTATTTAAAC1080.11512018333955125No Hit
ACAACTGGGATGGCAACTACACAGGATGTTGCTTACCAGGACGGAGTTTTGG1080.11512018333955125No Hit
ATTGATGGGGTTTAATGATACTCAATATAAATATTAAAAGAAGAGGAAACTT1070.11405425571603689No Hit
TGTTCAGGGCAAATATCTTTACTTTACTGTGGGTTTTTAGTACATCTGTTAA1070.11405425571603689No Hit
ATTAGCGGGGATATTGAATATGCCACAAAGTAGACTAAAATACTTCAGATCA1070.11405425571603689No Hit
TTACGTGGCCGGTCACTGGTAACAATAGCAATATGTGTCCAGGGACACAGAA1070.11405425571603689No Hit
CCGATAGGGACACAAAAGATGCAATGCCTATCAACAAAGCTACAATCATGCC1060.11298832809252253No Hit
CCTCAAGGGATGTCTGACTGACCCGGGCTGCTTCCGAGAAATTGATGAGCTA1060.11298832809252253No Hit
CGACCAGGGAAGCTTCTGAAGTTCTTTTTACTTCTCCTTTCTTTGTACTAAT1060.11298832809252253No Hit
GTTATGGGATATTAAGTTTAATTTAGTTATTTAAGGAAGGACTATTTTTTTT1060.11298832809252253No Hit
ATCGCAGGGTATACATGCATTTTGAAACTCGACATTATGACTCTCACTAGCT1050.11192240046900814No Hit
TAACACGGGGAGGCCCCAGGCACCAGGAGGTATACCCTTGCAGAGAAGCGGG1050.11192240046900814No Hit
TAAGCCGGGTTCTAGCATGTATTAATTTTGCAGCAAACATTACATCCATAAT1050.11192240046900814No Hit
CATAGTGGGGTGTCTAGGCCGGTCTAATGCCTTACCTCAAACACTCTTCTCG1050.11192240046900814No Hit
TTATTGGGGAATCACAGTGACAGATGCAGATGCTGGAGAGAATAAAGCTGTG1050.11192240046900814No Hit
GCATGGGTCTGACACTTGAAGTAGGAAACCATTTCCTTTGTCTTGGCCTTAC1050.11192240046900814No Hit
GGCTCGGGGGATCCTGCCACCTCAGCTTCCCGGGTAGCTTGGACTGCAGGCG1050.11192240046900814No Hit
CAGCCAGGCTGATGAAGAGAAACATAAACGTCAAATGTCTGTCCCTGGCATC1050.11192240046900814No Hit
AACACTGGGCACTAATTTTGAAACTGGAAAGCTAATTAATGACATATTCATG1040.11085647284549378No Hit
GCTGGCGGGACTGTAAGCAAAAGACAGCTGCTAGAGTTTTTTAACCCTAGAA1040.11085647284549378No Hit
TCTAGTGGGAGTTAACCAGAAAATTAAATTAATTCTTTAAACATGATGGTCA1040.11085647284549378No Hit
TATTGAGGGGGACTTAATTAGATGCAGTTGTCTTTATCCTGAAAGTAGTGAG1040.11085647284549378No Hit
CTGTTCGCGGGATCGCATGTTTTACCATACCAGACATTGGTTTGATTCTGAC1040.11085647284549378No Hit
GTGTCGTCTGATGAAGGAGAAACTTGAAGAGGAAGAAAACCTCACCCGGGAG1040.11085647284549378No Hit
GTGTACGGGACTCCAGGCAGCCCTTGTCCACGGGCTCTCCTTGCATCAGCCA1040.11085647284549378No Hit
TATCCGGGGGAAGGTTCATGGGGAAACTCGCTCCTTCTGTTGGCAGAGGACA1030.10979054522197942No Hit
CAGCCTGGGCGACAGCAGAGTGAAACTCTGTCTCAAAATAAATAAATAAATA1030.10979054522197942No Hit
GTTACTGGGTTAATTCAAAATCTTATGCTGTATTATCAAAACTATATCACTC1030.10979054522197942No Hit
ATTGCTGGGCGATGGGGGGGTGGAAATGTGGCTAAAAAGAATACTGTGAATG1020.10872461759846508No Hit
TTCACGAGGGAGGACGACTGAAAGAAGTTGCACCTTGGCCTCCTTTGAGCTG1020.10872461759846508No Hit
CGTATAAGGGAGAATGGCAATGATTAAAAAGTCAGGAAACAACAGGTGCTGG1020.10872461759846508No Hit
TTTAGCGGGGGAAATGCAAAAAAATTTTTATCTTGGCCAATGTCTTAAGGAT1020.10872461759846508No Hit
CTTTGTGGGTCTCAGATTAAAATTCCCATCAGTAGTAGCCATATTGAATTTT1020.10872461759846508No Hit
TCCTTAGGGGTCGTTTTTTCAGTCCCTGCTTCAGTTCTCAGATTCTGTGTTG1020.10872461759846508No Hit
GTCAGAGGGATCTCGAACTCCTGACCTCAGGTGAGCACCTTGGCCTCCCAAA1020.10872461759846508No Hit
AGCCTGGCTATTGGAGAGTGTGGGTTTGGAACTTGAGGCTAGGAGGTTCTGT1020.10872461759846508No Hit
GAAAATGGATCCCTGGTACACTTCTGTGAGTAGGATATATATAAAATCACTG1010.10765868997495069No Hit
TTACTTGGGACTTTAGAAACCCCATCTGTTGTCTTGGTGCTGGATATTTCCC1010.10765868997495069No Hit
ATGTATGGGCTCTGCTTTATTTTGAGGAGAAAAACATGAAATTTAATAAACT1010.10765868997495069No Hit
CAACATGGTGACTTGAGCTGATGATGCCGACCTGGCAGACACTAAACTCGTG1010.10765868997495069No Hit
AACATAGGGGATTGAACCTTTCCTCTACTTGATGCTTAGTTTGAGAACAGTT1010.10765868997495069No Hit
GTCAAAGGGGCATCTGTTTTTCAGAAGACTTCTATACAGAATTCTAAATTGT1010.10765868997495069No Hit
AATGTAGGGTTGCCAGAATGGGGCAGCATTTCATTCCATAAAATAGAATATA1000.10659276235143633No Hit
TTGCCAGGGACATCAATTATGGAGACTACCACGACCCTGGCTACTAGCACAG1000.10659276235143633No Hit
TCTCGCGGGGTGACTTTTATTTCTCATAACAGAGGTCAGAATAGAGAAAATG1000.10659276235143633No Hit
CGAGCTGGGCTTTCCAAGTTACCTGACCTTAAAGATGCTGAAGCTGTTCAGA1000.10659276235143633No Hit
ATCGTAGGGGAGACCATGAGTTGCTCCCGTTTGTTTAAAAGCAGGCTTTATG1000.10659276235143633No Hit
TCGTTTGGGCCGATAATCCGACATTGGCTTTAAAACTCATAGGCTTACTGGG1000.10659276235143633No Hit
CTAATAGCGGGGTTGCCAGCATGTAGAATCATCAAACTGTACTTGAAAGAGA990.10552683472792197No Hit
ACCAAGGGGGGGGCCCGAGGGACCCACACAGTGGGGGCCAGCCTCGCTGGAG990.10552683472792197No Hit
TTAGCAGGGTGGATGGCTGTATGAGGAGCTGGAACTGGCTGAACGGAGAAGA990.10552683472792197No Hit
CTTGCCGGTAAGTGGGGTGTGTGTGTATGTACATTTTAATAAAAGTACTAAT990.10552683472792197No Hit
AATTGTGGGAGGAAATAGAAACCGTCTGAACTATCCTGCCCGCCATCATCCT990.10552683472792197No Hit
CTTAGGCCGGGAAGTCAGTTCTCCCTGGTCCAGAGATTGCAAAGTGTAATTA990.10552683472792197No Hit
GGCTATGGGATTAGAACAAGATGTTGCATGAGCAAAGGACCTAAATTGTTAT980.10446090710440761No Hit
TACAAAGGGCGGAGACACAGGGGCTCAACAGAGTGCTTCCTAATAGCTTTTT980.10446090710440761No Hit
GGCTCGGGGCTTCCAAGACCAGATAAAGTCTTTGTACTCTAGATGTGTGTCA980.10446090710440761No Hit
ATATAAGGGAATAGTGTGGTCTGTTCTTTCTTGCCAGAAGTTATCTACAAGA970.10339497948089325No Hit
ATGCAAGGGGCCTCCACTAACCCCCTGCCCCTACGCTGTTCCTGAAGTCCCA970.10339497948089325No Hit
TGTTAGGGGATACTGACCTCAGACCCTTGGAGGAAAAACATGTTTTCCAATT970.10339497948089325No Hit
GCTGTTGGGATGTGTCACTGTAGGATTTTTTCTTCAAAATTGTATTTTTGAA970.10339497948089325No Hit
TGCATCGGGATATTAGTCTACAATTAAGCAAGGAAGGCAAAAATTACCTAGA970.10339497948089325No Hit
CGAAGAGGGTTAGACACTTTTTACTAAGAGATAACTTAGTGCCATTTAAGGA970.10339497948089325No Hit
CAATATGGGTCTCTGATTCATGAAGAAGTAGAGTTTGGTTAGCTCCATTTAA970.10339497948089325No Hit
CGGCTTGGGGATCTGCTGAAGGGTATCTTTCAAGAATGTTTCAAATGAGAAC960.10232905185737888No Hit
GACCAGGGAAGTACAAATGAGGCTTTGTACAAGCTTTACAGTGCAGCAGCAA960.10232905185737888No Hit
CGCTATGGGCTACTAGGAAACCTTCCTCATATGAATAACCATTTATATGTGT960.10232905185737888No Hit
GCTGGTGGGAAGCAAGAAGAAATAAAGTTCAGATCAGACTAGTTAACACTTG960.10232905185737888No Hit
TTACTAGGGCTGGGGGCCAAATGGTACCCTGATTATTCCTGTGAGGGATACT960.10232905185737888No Hit
TCCACAGGGCATCCCACTGGCTCGCGGGGGCGTCGTGAGGCAGTACTGGAGC960.10232905185737888No Hit
ACTGCTGGGCTAGCTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTG950.10126312423386452No Hit
GCACGCGGGATATTCTCACAGTTTTTCCCCAGGAAGTCTGGAGAAAAAGTCC950.10126312423386452No Hit
ATGACTGGGAACTGGTAAAAATATTTACCCGGAAATGAAGAAACATTAAATA950.10126312423386452No Hit
GCGCCGGGGCTTTGCTGCTGGCTTTCCTCTAGGTGGCGAGTGTACTTGTTTG950.10126312423386452No Hit
TCATTCGGGAGGGAAATAAGCAACTACCTTGAATTTTTGCATGAATTGCATT950.10126312423386452No Hit
GCACTAGGGTCACATTTGTAGAAATGTAATGCGGTTCTGAGTGGCTTGAAAT950.10126312423386452No Hit
TGTACTGGGGGCGGCTATAAGAGACCACAAGCGACCCGCAGGGCCAGACGTT950.10126312423386452No Hit
AGCAGTGGGTATAAAAGAAATAGCCAAATAAAGAGATAATCTGTCTCTCCCA950.10126312423386452No Hit
GTATAAGGGGGATGCTGTCTTCTTCCACAGGTATGGCAACAATTTCTTTCAC950.10126312423386452No Hit
GTGGAGGGGATGAAAAAGAATATGGCAACAATACCTTTTAGCATTGAAACCC940.10019719661035016No Hit
TTACGGGAGTTATAGCAACCTGTGCAATTAAGCTCATTTTCGGATAGCACAG940.10019719661035016No Hit
AACTTAGGGTACTATTTATTTGTTTTAAGGCACTGCCTTGATCCATGTTTGC940.10019719661035016No Hit
TCACCGGGATATTGAAGAGGACGGGTCTAATAGATCGCTGGAGACACAATTT940.10019719661035016No Hit
AACTCAGGGCGCTCTTCCGATCTTTACGTGGGGGTTAGACCGTAGAAATTAT940.10019719661035016No Hit
CCAGATGGGTTAGTGATATGATATTTGAGTCAAAAATCTGAGGTTATAGTAC940.10019719661035016No Hit
GCTGACGGGCAGCAGGTGCTCAAAAAATATTTGAATGGATAAATAAATGATG940.10019719661035016No Hit
ATTTTAGGGATTTGAAGAAATTATGAAAATTCCGATGAGTTTTTAAAGTTTG940.10019719661035016No Hit
GACTTTAGCCCCAGCCTTGTCACTCTTTTTTTGATGTCAACAAAAAGTGGAA940.10019719661035016No Hit
AGTGCTGGGATTACAGGCGTGAGCCACCGTGCCCAGCCTGGTTTTAGCTTTT940.10019719661035016No Hit

[OK]Adapter Content

Adapter graph