FastQCFastQC Report
Sat 31 Dec 2022
SRR5843868.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843868.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117273
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGC6060.5167429843186412No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGCCGTCT3730.31806127582649035No Hit
TATCACGGGGGATCTTACAGAAGGGCAATGAAAATAATCCTTCAAGAATTAA2020.17224766143954703No Hit
CTGTATACGGGGGGAGGGGGCGGGGCTTCGGCCGGCTAAGCCGCGGCCACCC1940.1654259718775848No Hit
GCCGATGGGGGCGGAAGAGGTGGCCCCGGATGCTGCGGCGCCCGCTGGCCGG1800.1534880151441508No Hit
TACAATGGGATACACAGTATGTTCTCTGACCACAATAGAATGAAATTAGAGT1780.15178259275366027No Hit
GCGCATGGGATAAAGAGAAATTCTTAAAGAAGCTAGATAAAGAAAGGCAGCC1750.14922445916792443No Hit
CTATCAGCGGGTTTCTGGAATTCCTGTTTTCCAGATGTTAGACCTCCAGAAT1710.1458136143869433No Hit
AGCACTGGGACTATGAAATTTGAGATTTAACATTTATTCATTAGTCAGATTT1700.144960903191698No Hit
CCTCAAGGGTGCATTTTTGCTTTTCATTATCTTTTTTTTTTTTTTTTTTTTT1690.14410819199645272No Hit
GCTGGCTCTATTTTTACATTCCCTTCCACCTCTATACTGTCATGTCACCGTT1670.14240276960596215No Hit
TGCCAAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1640.1398446360202263No Hit
GAGCCAGGGTATTCTGCTGGAAGCCCATGAACCATCGCTGAGGTTCTGATAC1630.13899192482498104No Hit
CCTTCAGGGCCACTTTTACTAAATTTGCTCTTTCAGTCTACTCATTTGTTAT1610.13728650243449045No Hit
GAATGTGGGGAATTATAATTTAAACTGTTTAATTTTGTTTAATGTGTATATT1600.13643379123924518No Hit
GAAGCAGAAGAAAAAAAGACAAGCTAGGAAACAAAAAGCTAAGGGCAAAATG1570.13387565765350934No Hit
GACCACAAAGCCCATGGTAACTTCTGCTTGTAATGGAACACGGAATGTGGCC1570.13387565765350934No Hit
ACAACGAGGGAGGTGAGCGGGGGCCACAGCCCGGGCTGGGGGGAGCCGGGGT1570.13387565765350934No Hit
GACAGAGGGGTCAAATTACAGCTTCTTGTGTTCACCACGGAAGTAAATGATC1560.13302294645826404No Hit
TTTTAGGGACACTGGGCCCACATTAGAATGGGATAACTATGATTTAAGGGAA1550.13217023526301877No Hit
TAACTAGGGATCTCTCTTTTTTTAAGTTATTTGAATTGGGATTCAAATTCAG1550.13217023526301877No Hit
GACAGTGGGATAAACTTAAAGTAAAGGGGTGGAAAAGACTTTCCATGCAAAT1540.1313175240677735No Hit
TCTCCGGGGTAAATGGGAAATGTCGATTTTCAATAATAGACTTATATGCAGG1540.1313175240677735No Hit
TACTAAGGGGATGGAAGGCGACGCACAAAAGCTTGAAGTAGAAAATGCCATG1530.1304648128725282No Hit
AGTCACGGGCACGGAAAAACAAAGGGCAAGATGGAAATAAGGGATGAGAAGG1520.1296121016772829No Hit
GTTACAGGGAAGGTAAATTAAATATTTTTACAGTTTCTTACTATAGAGCTTG1510.12875939048203763No Hit
CGAAAGGGGAAACCTACCACACTGCAAGTCATAAAAAAGTCTAACACATGCA1510.12875939048203763No Hit
TTAAGAGGGATGATCAGTGGATAAAATCACAGAAAATTGTCAAATTTCCTCG1500.12790667928679236No Hit
TCATATGGGATCAGAAGAATGTCAATTGGCAAGAAAATCTAGTGGAACAGGC1500.12790667928679236No Hit
GATCCTGGGCAGAATGTGGAGCACTGAGCAGGACAGCTGACCTGTCTCCTTC1490.12705396809154706No Hit
AGTCATGGGGGGCGCCGGGCGCGTCTTAGGGTGGCGCGGCGGGAATGGTTGG1490.12705396809154706No Hit
CAACTTGGGTATTTCAAACAATTGGCTCACATAATTATGGAGGCCAACAAGT1470.12534854570105652No Hit
CACTGGGGGATGAGACTCAACTAGAAAACACCTGGTCAAACTTAAGCATAAC1460.12449583450581123No Hit
TGTCATGGGGATCTTAGCGAGGGGTTTAAAAATGTGATTCAGCATGATTCCA1450.12364312331056594No Hit
GCCCAAGGGTACCTTGGTCAAGAAATGGAACCACACCTCCAAGAAAAGCTGA1440.12279041211532066No Hit
TACATAGGGGCTCTTCGTTAAGTCGGCCTTCCCAACATGGCGCAGTCTATTA1440.12279041211532066No Hit
GCTAGGGGATTTTGGTGCAGTGAGAATTTATAGAAGTAACCAGCTTGATGGA1440.12279041211532066No Hit
GTTAATGGGTACATATGGCGAATAGCACATGGAAAAATGTTCAATATCATTA1440.12279041211532066No Hit
GGTTAAGGGTTATTTTTGTTCTTCAGTTTCATACATATTTTGACTAGAACAT1430.12193770092007539No Hit
GCCCGCCTTGGCCTCCCAGGTGTTGGGATTGCAGGCGTAAGCCACCGCGCCT1430.12193770092007539No Hit
GGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGA1430.12193770092007539No Hit
TGCTGTATTTTGAATGTTGAAAAATTAGAAATGTATGTATTTTTAAATAGTA1430.12193770092007539No Hit
TAAAACGGGTGCCACAGGCAGCACAGCAAGCAGGTTACCAGCCAGTCTTGTC1420.1210849897248301No Hit
TTTCTTGGGGACTACAGGTGTGCGCCGCTTCACCAGGCACCATATTGTTTTA1420.1210849897248301No Hit
ACTATGGGGACAAGAAGAAGTCTGGAAAGTATACAAACACATTGAAATCAAA1420.1210849897248301No Hit
CATGCAGGGCTCCTTAATATGTTTGAAATTATTGATTTTGGCATTTTTCTAA1420.1210849897248301No Hit
TATTAAGGGGTTTTGAGTGAGTTTCTTAATCTCGAGTTCTAATTTGATTGTA1420.1210849897248301No Hit
GTATTAGGGAAGTAGAAGAGCAGCGTGAACAGGAAGCTAAGCATGAACGCAT1410.12023227852958482No Hit
AGCTTAGGGACTGCTTTAAGTTTCATATTCAAAACAGTTTTTGTTTTTCTCT1410.12023227852958482No Hit
CCAGGCGGAGGGGCTTGGAGCAGAGACGGCAGCCCCATCCTTCCCGCAGCAC1410.12023227852958482No Hit
ATAGCAGGGATAACCCATTTATTGCCTTCTGGCAAGAAACTGGCGCTTGCTG1410.12023227852958482No Hit
TGGCTAGGGAAATTCAAACAAGACTCTTTCCAATTTAAAGGGCCAAACCCTA1410.12023227852958482No Hit
TTTGCTGGGAATCTCTGCTTTTCTAAAAGCACTTTGGTCAAGTCACTTAACC1410.12023227852958482No Hit
AACAACGGGGCCGGCCGGGGGCGGACTGGGAAGCCGGCAGAAATGGAACGAT1400.11937956733433952No Hit
GTTCGTGGGGATGCCCTTCTGAGTTGCTGCTATGCCAGTGAAATGGAGAGAT1400.11937956733433952No Hit
GACAATGGGATTTAAATGTATTAAGGTTTTGATGAGTTTAGGAAAATACTGA1400.11937956733433952No Hit
TCGACAGGGAACTTATTTTTCATAAAGTAATCACATGTTGATAGAAGCTTTT1400.11937956733433952No Hit
GCACTGGGGCTTTAGTTTTCTTATTGTGTTGCCTAAAGTATTGCATCATTTA1400.11937956733433952No Hit
AGGTTCGGGCTCCACGATCCCTCATTTTCTCTCTTTGCAGATAATTTCAAAT1390.11852685613909426No Hit
CTAGCTGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACT1390.11852685613909426No Hit
GCCCAAGGGCATGGAAGAAGGTCCCAGAAAGGTTCCCAGGAGATAGGTGTCT1390.11852685613909426No Hit
CATCAACCCCTTACTATAAAATGAATAAATAACCTGCATTTTGTATAGAAGT1380.11767414494384897No Hit
TCCTGCGGGACACTAATGGAAGAAATTAAAAAGGACACAAAACAGTGGAAAC1380.11767414494384897No Hit
TGCCTCGGGGCGGCACTACAGAGCTCCCGAGGTCATTTTGGCTTTAGGTTGG1380.11767414494384897No Hit
TAATCTGGGTTCCTACTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACG1380.11767414494384897No Hit
CATGTGGGGAGAGGTTATGCATTTTCCTTTAGTTATTTTATTTTGCTATACT1380.11767414494384897No Hit
CTCAAAGGGCAGGTTTTGTTTTAGTTTTTGGCTTTTAGTGAGCTTGCATTTC1370.11682143374860368No Hit
CCCCACCTGCCTAGTACTCTTACATGAGCCTTTTACATTTTTTTAACCCATT1370.11682143374860368No Hit
CCACAAGGGGTGGTTTTTTACAGGAGTTAAAGTACATAAATGCCTTTTTACA1370.11682143374860368No Hit
TTTTCCGGGACATGAAAGGAGTGGGACTGATGCTGGACAGGGCAGCACAATG1370.11682143374860368No Hit
ATAACGGGGCCATGTAAAAATCCTTTGATATTGCCAGGAAACAACTTTAAAT1370.11682143374860368No Hit
TACGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1360.1159687225533584No Hit
TGGTACGGGGATCTTCTTGCGGGAACCCAAACCCAGCAGAAGAAAAGAAATA1360.1159687225533584No Hit
TAATGCGGGGCACGCGGCCATGGCCGACTCCGAGCTGCAGCTGGTTGAGCAG1360.1159687225533584No Hit
CTCTTTGCAGGGAAGTCAAATTATCTGTTAAAAACAAACTATAAATAAATAA1360.1159687225533584No Hit
TAATTAGGGATGGAGAAACTAGTCATGGGCCAAAGGTCAAGATACTTCTCTG1360.1159687225533584No Hit
TTAGCTGGGGAGCCGTGCTTATATAAAGCTTCAGGAAGCGCCGTTCCGACGA1360.1159687225533584No Hit
TGGTAGGGGCTGCTCTAAAATTGGTGTTTGGGTGATCAGAAGCAGGTAGCCA1360.1159687225533584No Hit
TCAACCGGGACATAATGTTGAAATTATTGATTTGATTTCTCCATTAGATTTA1350.11511601135811313No Hit
TCTCTGGAGGGATTAATGTACTTTTTATTGCATGGTGTGAACTAAGTTATTG1350.11511601135811313No Hit
TGTTCTGGGCTTTAAGAACAGGTATTATTTACTAAAGGTGAATTACCATGGC1350.11511601135811313No Hit
TCTATGGGGTCCTTGAAATTGAGTTCCTTTGGCATTTTTCCTTGCAGTCATC1350.11511601135811313No Hit
TCGCGCGGGTATGCTGTAGGGGACAGAGATTTCTGTCTTGATTTCAGGCATG1350.11511601135811313No Hit
TTCCAAGGGCTGGACTTCTAGAAACAAGTGAAGTGTGCTAAAGTCTCCTATT1340.11426330016286784No Hit
GCTAAGGGGGATGGTAGAAAGTGAATACACTGCATATAAAACATTTTGAAAT1340.11426330016286784No Hit
ATAACAGGGGTGATTGTCTGGGGGGATTCCTGGGTTTCCCTGGGTACCTTGT1330.11341058896762256No Hit
CTTGCGGGGCAATGAAACTTGCAGAGGAACATTTCATGCCTTTAATTAGTTC1330.11341058896762256No Hit
TAGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1320.11255787777237726No Hit
TTTCCTCTACCTCCTTCTCCTATTTCACCTGATACTAGAATTCCTCCTTTCT1320.11255787777237726No Hit
TGCTATGGGGACAGTTATTGGGAGTCTAGCAAGATGTCTTTCATTCTGTTCC1320.11255787777237726No Hit
CAACCAGGGCAAAAAAACCACTCTAGCAGGGCATGGAGAATAGATGACTGAT1320.11255787777237726No Hit
ATTCTTGGGGCTGGCAGGTGGACCAGGCCATCCTCTCGGAGGAGGACCGCGT1310.111705166577132No Hit
GCACATGTACACCTCAACTTAAAGTTTAAGAAAAAAAGAATGCTGGTGGGAA1310.111705166577132No Hit
AAAATGAGGAATCTTTTTGTTTCTCTTTAATTTTTTCATTGACCATGTTGTT1310.111705166577132No Hit
TATTATGGGCCTCATCTCAAAAAAATAAAAAGGATGTACTTCAGAAGGATGG1310.111705166577132No Hit
TGGATGGGGAGAAGGGAAGAATTTAAAAAATGAGATAATTCCGGTGTCATTG1300.1108524553818867No Hit
GAAAAAGGGAGCTCATTTTATTTATTCATTAAAAAATTATCTTATTCATCTT1290.10999974418664142No Hit
GTAATCCCAACTACGTGGAAGGCTGAGGCACAAGAATTGCTTGAACCTGGGA1290.10999974418664142No Hit
GACACAGGGACTGGAAAGCAACATTAGAGTTGTCCTCAGATGAAAAGGAGTT1290.10999974418664142No Hit
AATCCGGGGGTAGTGACATAGGCGTTTGTGTCACTCCTCCCTCACCTCCAGG1290.10999974418664142No Hit
CGGGCTGGGATAAGTTCTTTTCTTTGGTAAAATTTTCCAGTGAGTTCTAGTC1290.10999974418664142No Hit
AGAGTAGGGGTCAGTTCCTATGGGGAGCGCTTTAGAACTTTCTCTCCTTGTG1290.10999974418664142No Hit
TGGTTGGGGAGGGATATAGTGGGAAAAAAATGCTTGAATAGGAGTCTAAAGA1290.10999974418664142No Hit
GAAATAGGGAGATGTAATTCAATTTGACTAGTGTTTATTAAGTTCCTAATAC1290.10999974418664142No Hit
TGAATCGGGGGGAAACAGCATGTGCCTCATGAATTTTCTTTTTTGTTAATAA1290.10999974418664142No Hit
AAGCAGGGAGTACCCTTCTGGCAAACAAATAACTGAATTGCTACTATTTAAC1290.10999974418664142No Hit
TCACATGGGATCAAAGGAGAATTTTTAAAGCCTTAACAATGCCTACTTTCCA1290.10999974418664142No Hit
GCCACTGGGACAGCCTTCCACTAACATCCCGTAGGAGCCTCTCTCCCTACTG1280.10914703299139614No Hit
CTATCAGGGCTAGTAAAATCATGAAACTCTTGATACAGTTTTATTTGCATTT1280.10914703299139614No Hit
GGTTATGGGGAGATGTGGGATCTCTGGATTTGAGGCTGTACAAGTGGTACAG1280.10914703299139614No Hit
CAGGCTGGGGATTTTGTGGAAGTTCTGATTTATGGCTGAAAGAGTTGCACCA1280.10914703299139614No Hit
GCCAATGGGGATCCTGGCTTTTAAGAAATTATTATTCCTGCTCTGCTGAGAA1280.10914703299139614No Hit
TACGCGGGAAGAGACAGGCAGGTGATCTGCTGCAGAGCAGTAAGGGAACAAG1280.10914703299139614No Hit
CAATGAGGGACTGAAAGTCCACTCCTACTCATCACAGGGCCTTTGCACATGC1280.10914703299139614No Hit
TAGCCCTGGGTGGTACTCAAAAGAATGAAAGGTGGTCCCTACATTTTCTGTA1270.10829432179615087No Hit
GATTAAGGGATTACTAATTCAGATCCCTTTTGGTACTTAAATGTCACCCTAG1270.10829432179615087No Hit
CTAATTGGGACTTGAATCCTTGTCCCAAGTGGGTCCAGTAAATGAGAATGTT1270.10829432179615087No Hit
AAACTAGGGTCTGCCTTGTAAAGTTCTATCACGGCCTACCTGGAATTTAAAA1270.10829432179615087No Hit
GTATCAGGGAGTCTTTCCAGCTAACAAGGATTTTCAGCATAATAAGTAGATT1270.10829432179615087No Hit
TACCATGCCAGAGGAAGAAAAGGAAATGTGCAGAAGAAAAGGAAATGTGCAA1260.10744161060090558No Hit
AGCAATGGGGTATTTTGGATCAGTATTCTTTTCATTCTCTATGTAAGTGACA1260.10744161060090558No Hit
TACCCGGGAAGAAAAGACTATAACAATACTATAAAAAATTTAATGCCACTAC1260.10744161060090558No Hit
TCCAGAGTGGGTAAAAACTCAAAGTAATAGTCTCAAATTCAAAGAAACATTT1260.10744161060090558No Hit
AATACGGGGTAACAGAATTTACAGGACATTTTACTCAAGAGCTTCAGAATAT1260.10744161060090558No Hit
TGCTCAGGGTAGAACTCTATTTTCCTGCTACAGAGTAAAAAGACAAAATTTC1260.10744161060090558No Hit
TGTACTGGGGTTCACCAATCCTACTTTTGTTGCTCTTTTCTGCCTTGTTTTC1250.1065888994056603No Hit
TAGCCAGGGAATCACACATCTGAGAAATTACTTTCACAAAACGCTCTACAAA1250.1065888994056603No Hit
GTTCTAGGGCTCTTTTTACTGAATTAAAGTTCTACCAACGAGCTGCAAAACC1250.1065888994056603No Hit
TCAGCTGGGAGTTTGCATTTCCTAGACATACACTGATTGGACACACAATTCT1250.1065888994056603No Hit
ATGTTCGGGGTCTGTTGCTCGAATAACTCTTACTTTAATAACTGGGGCTCCT1250.1065888994056603No Hit
TCGAAAGGGTGTAACATTTGGAAGATTTTGTTATGGTGTCACTGTCATTTTG1250.1065888994056603No Hit
ACTGCAGGGTAGGTTATTTTATGTAAGTAGAAGTCATAAGACTATCACATAA1250.1065888994056603No Hit
CTAACAGGGGTTAATTTATTATTGACAAAAGAAAAATACTTTTTACAAATAT1250.1065888994056603No Hit
GTTTCAGGGGAAGATGTAGAACACTGGGAACTGTCACCAGGAAGGATGTGAT1250.1065888994056603No Hit
TTTACCGGGCTCGTGGAGTATTACTATAAGATGAAATTTTTTACAAAGTGTG1240.10573618821041501No Hit
ATTTTTGGGGTCGGATGAGAATGATGTTTTTGACAAGCTAAGTGTCATTGCA1240.10573618821041501No Hit
AAGTTTGGGAACTATACAGCTTGTCAGTGGAGGTTCCAAGATTCTTACCACT1240.10573618821041501No Hit
TGACTGGGGTCTTGGAACGGGAGCTATGTGATTATAATGTTTTTGGTGTGGG1230.10488347701516974No Hit
GCCGTGTTATACTGTTTCCCAATGGAACTCGAAAGGAAGTGAGTGCAGATGG1230.10488347701516974No Hit
TACAGCAGAATTTTAAAAAAAATAGATGTCTCTATCTTCATTCCTACATTTC1230.10488347701516974No Hit
AAAGTAGGGTATAAATACCATTAAGAGTTAGATACAAGGAGGTTGAGAAGCC1220.10403076581992446No Hit
TTATAAGGGGCCCCCTGGTGGTTACGAAGTTATCTATTTAATCTGTCAATAA1220.10403076581992446No Hit
TGACCAGGTGTACTTGGTAGATTATGGCCTTGCTTATCGGTACTGCCCAGAA1220.10403076581992446No Hit
CCTCCTGCTTGTACTAAGCCAGGAGGCTTTAAGCTCCAGCTTTAAGGGTTGT1220.10403076581992446No Hit
CAATACGGGGATCTGGAGGAGACTCCAGTGAGCTGGAAGGATGACACTGAGA1220.10403076581992446No Hit
CAGCTCGGGGTCATAAGCTCCTTGTGAACCCAAACATCCCATCTCGACACAT1220.10403076581992446No Hit
GTATCCGGGAGCTCACTGCAACTTCCACCTCCTCGGTTCAAGTGATTCTCCT1210.10317805462467916No Hit
GCTACTAGGTTTTGAACCAAGTAGCAGGCAGAAACTAAAAGGGCATTGAAAA1210.10317805462467916No Hit
GGAATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1210.10317805462467916No Hit
TTTTATTGGGAGATTCTTAATCTGACTTACATGTTACTTTATCCGTATGTCT1200.10232534342943388No Hit
ATACACGGGAAACTACCATCAGAGAATACTATAAACACACTGTGCGAATAAA1200.10232534342943388No Hit
GATGCTGGGGTGAAAAGAGGCAGGAAGAGAAGTGTCAGGGAGTCAAAGATAG1200.10232534342943388No Hit
GTTGCAGGGGTTTCTAAGAAACTGTATGACCAGAAATGAATTGTCTTTGATT1200.10232534342943388No Hit
GTTACTGGGAAATGTGTAAAGAAATTGAGCTCTAATGTAAGTTTCTATAAAA1190.1014726322341886No Hit
AACCACGGGACTGGGGAGTCTCTTTTCATGTAGGTTCTCCTTGATCATTTAC1190.1014726322341886No Hit
ATTGTAGGGTGCTTCTCGGAGGAAAATCTTAATCTAGACAGATTCAGATTCC1190.1014726322341886No Hit
CTTGCTGGGCTTTTCTCTTCCGGTTCTAGGCGCTTCGGGAGCCGCGGCTTAT1180.10061992103894332No Hit
CTGCTTGGGGAGATGTGCATAAACAGAGAAGCTCAAAGGGAGGCAGAGAAAC1180.10061992103894332No Hit
CAAAATGGGTGATATACCTTATAAAACAAAACCAGCGATGAGGAAGTGAGAT1180.10061992103894332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)