FastQCFastQC Report
Sat 31 Dec 2022
SRR5843866.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843866.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176708
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGC8520.4821513457228875No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGCCGTCT7380.41763813749236023No Hit
TTGCTTGGGGAAGGCACACCCGCAGAAGGCCGGCGTGCAGAAACAGGCTTGC1980.11204925640038933No Hit
TGTAACGGGGAGGCGCGGGGGTGGCGCGTCAGTTGCTTCAGCGTCCCGGTGT1960.11091744572967835No Hit
TACAAAGGGCAGGAGACAGTGAAGATGAGAGGAGTGACAGAGGATCTGAGTC1960.11091744572967835No Hit
ACTCATGGGTCCCTTGATTATTCTGGTTTTGCCATGGTGTTTAACCACTGGC1910.10808791905290083No Hit
TGAGTTGGGATCAGGATACGTGGCTTTAGTAAGCAGACCAAGCGCTTCGTGG1900.10752201371754533No Hit
ACTTGTGGGGACAGCGAGGGCAGCTCAGACAGCGACGCCGATGAGCTGGCCG1870.10582429771147882No Hit
ATCCAAGGGTCAGTTAGGAGATTTTCATATTAATCCAGGCTGGAGATCAGGA1830.10356067637005682No Hit
AAGCTGGGATATCCAAAATTTTCATGCAGTGTTTACCTCTCTTTATCTTAAG1830.10356067637005682No Hit
TCTAATGGGAGCTGTCTCTTGGAAAAATCTACATTCTGCAAAGAATCCCCTT1800.10186296036399031No Hit
CCGTGAGGGGAGATGTTCATAGTTCCAGTCGTGTCTGTACACCAGAGTGGGT1770.1001652443579238No Hit
GTCTGTGGGCGATCTTTGCGCGGGGGGAATAGTCAGAATGAAAAGCCATTCC1770.1001652443579238No Hit
GTGCAGGGGGCCCGCCCGCTCCGGAGCAACCCGCGAGCTTACACCGGCTTCT1770.1001652443579238No Hit
AGCCTGGGTGACATAGCAAGACCCTGTCTCTAAAAAAATTAATTAAAGTAAA1770.1001652443579238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)