FastQCFastQC Report
Sat 31 Dec 2022
SRR5843865.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843865.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177066
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGC8090.4568917804660409No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGCCGTCT6770.38234330701546315No Hit
TACAAAGGGCAGGAGACAGTGAAGATGAGAGGAGTGACAGAGGATCTGAGTC2410.13610744016355483No Hit
TTGCTTGGGGAAGGCACACCCGCAGAAGGCCGGCGTGCAGAAACAGGCTTGC2400.13554267900105046No Hit
GGTTGCGGCTTTGTTAAATATTTTGAAATGTAAATGAAAAGAACTACTGTAT2060.11634079947590165No Hit
CTTTATGCGGGAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTTA1980.11182271017586662No Hit
GCAAAAGGGCTGGAAAACAAAGATGAAAATAGAATGCCAGAGTGATTTTGTA1970.11125794901336225No Hit
TGTAACGGGGAGGCGCGGGGGTGGCGCGTCAGTTGCTTCAGCGTCCCGGTGT1960.11069318785085785No Hit
GTACTTGGGGAAGAAGGCAAGGCTGGAGGCAAAGTTATTGCTAGTATCAAGG1960.11069318785085785No Hit
TCTGGTGGGCTGCAGTAGGGCTTCCATTCATCCTTTCTTGGGAGAAGGCTGT1950.11012842668835349No Hit
ACTCATGGGTCCCTTGATTATTCTGGTTTTGCCATGGTGTTTAACCACTGGC1800.10165700925078784No Hit
AGCCGAGGGGACCAGGTGGAAATCCTGGAGTACAACTTCAACAAGGTCGACA1780.10052748692577909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)