FastQCFastQC Report
Sat 31 Dec 2022
SRR5843864.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843864.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176345
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGC8010.4542232555501999No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGCCGTCT6610.37483342311945334No Hit
TTGCTTGGGGAAGGCACACCCGCAGAAGGCCGGCGTGCAGAAACAGGCTTGC2180.12362131049930535No Hit
GCAAAAGGGCTGGAAAACAAAGATGAAAATAGAATGCCAGAGTGATTTTGTA2070.11738353795117526No Hit
TACAAAGGGCAGGAGACAGTGAAGATGAGAGGAGTGACAGAGGATCTGAGTC2000.11341404632963792No Hit
TGTAACGGGGAGGCGCGGGGGTGGCGCGTCAGTTGCTTCAGCGTCCCGGTGT1870.10604213331821147No Hit
CTCAAGGTGGGTGAGTTCCTCATTCTTCTGAGTGACAGCCCGGACTTCTACA1820.10320678215997052No Hit
GGTTGCGGCTTTGTTAAATATTTTGAAATGTAAATGAAAAGAACTACTGTAT1800.10207264169667413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)