FastQCFastQC Report
Sat 31 Dec 2022
SRR5843863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84403
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGCCGTCT4200.4976126441003282No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGC4200.4976126441003282No Hit
ACAACTGGGTACCCGAGACTGTGTAATTTATGACGAAAAGAGGTTTAATTCC1310.15520775327891187No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1260.14928379323009847No Hit
TATACCTGGGTGTTGCAGTGAGCTGAGATTGCATCACTGCATTCCAGCCTGG1250.14809900122033576No Hit
GAAAACAGGGTGGTGGAATTTTTATTGAAAAAGAAAGCAAGTGCACAATGCC1240.1469142092105731No Hit
GCGCCTGGGGGAATGGGAAAGGAGACAATGGCAACAAAATGTTGGAAGCTGG1180.13980545715199696No Hit
CTACAAGGGCGGTAGCGCTTGCAGCATGGCTGACCAACTGACTGAAGAGCAG1180.13980545715199696No Hit
TACTGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1170.13862066514223428No Hit
AAGCATGGGGCCTGCAAAAACAGAGGTGGATACGAGGACAGGTTGGAGCTGC1140.13506628911294621No Hit
TGACCGGCGGTCAGTCTGCGGCAGCGGCAGCAAGACGGTGCAGTGAAGGAGA1140.13506628911294621No Hit
GGAAACGGGATGGTATTTACAGTGAAAGAGTAAATTAGAAAGTGAATGGATG1130.13388149710318353No Hit
TGCCTAGGGGTGAAATCAGTGCATTGATTAGGCAGAAGAGACATGAACTGGA1110.13151191308365817No Hit
TAACCAGGGGTAGGAGGGATTTCGGCCTGAGAGCGGGCCGAGGAGATTGGCG1110.13151191308365817No Hit
TGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1100.1303271210738955No Hit
GAACATGAGGGGCGGTTGGTCAGACCGGAGCACTTGGCCTGAAGACCTGGAA1100.1303271210738955No Hit
GGTACAGGGAAGTAAAAGGACCATAGAGGGGAACCAGGAATCTGAGAATCAG1080.1279575370543701No Hit
CAAGACAGGATTGGTGATTTTCTGGTATTTTACCACACTTCTGAGTTGATTT1070.12677274504460742No Hit
GTGTTAGGATTGAAGTAGACAAGGACTTTGAAATAACTGTTTGAAATATTGT1060.12558795303484474No Hit
GTCCAAGGGATGCAAAGTTATCAAAGAAGAGGAGAAGGAAAGGAGGACAGAT1060.12558795303484474No Hit
AAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1060.12558795303484474No Hit
AGATTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1050.12440316102508205No Hit
TTGCTTGGGACGCCTGGACTTTCTGCAGGCCTGGATCCAAGATGGCATCCCA1040.12321836901531937No Hit
ACCAAGGGATGAAAGCTGTAGACCCTGTCCATAGAAAAAATAGTGCACACTC1040.12321836901531937No Hit
CAACATGGGATTGAACATTTTAAAAATAGCTAACTTACAGGAGATTTGATAT1040.12321836901531937No Hit
AATTGAGGGAATGAGTCGTTCTTGGAATACCTATGTGCAGCCACTACCCATC1040.12321836901531937No Hit
TAGCTAGGGAGGACTGGAAGGTGGCTAGGGATGTGTAGCGGGAGAAGGGTCA1040.12321836901531937No Hit
GCCATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.12321836901531937No Hit
AATTGCGGGGCCAGCTTGTGAAGATGTGAAGGCCAAACCAGTATCCGACAAT1030.12203357700555667No Hit
TTGATAGGGATCGGAAAATTCATACTGGAGAAAATCTTTACAAATGCAAAGT1030.12203357700555667No Hit
TGCATAGGGACTGTCACTTTTGGACTGAAAAGCTGAGGAAGAAACTTAATTT1030.12203357700555667No Hit
AGCCATCGGGGGAAGCAGGTTAAATGTTTTGTAAACTTTGAAATATATGGTC1030.12203357700555667No Hit
GGCATAGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTG1020.12084878499579398No Hit
ATCAAGGGAAATCAGAGTCTTTAATTCTTGCATGTTTGTATCTAATTTCTGA1020.12084878499579398No Hit
TCTGCGGGGGTTTTCACTTTAGTGGTTCTGCCTTCTAGTCCACAGAAAAGTT1010.11966399298603131No Hit
GCATTGGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1010.11966399298603131No Hit
TAGATCTGGGATTGACATACAAATTCAGGTTTGAATCCTTGCAAAATCACAT1010.11966399298603131No Hit
CTAGCGGGTGCAGACAGATGTGGATTTGGATCCTGGCTCTGCAATGTAGTTA1010.11966399298603131No Hit
TTAGCAGGGCAAGAGAAGAGAAATTATTTCAGATGTGCCAGAATAAAACAGA1010.11966399298603131No Hit
GTGCAGGGGGAGCTCAGCCAGCCCTGCAGGGCGAGCAGTCCAGCCTTGTGTT1010.11966399298603131No Hit
TTTGATGGGAAATATTTTTAGTCCAGAGGTTTTAAGCTGTGTGTCCATTCCT1000.11847920097626861No Hit
GCGACCGGGGCATGAAGTTTCGTTGAAATGACAAATACTAATTTCTTCATCT1000.11847920097626861No Hit
ATTCAAGGGGGACATTGCTCCATCATTTACTAGGTAAGAGACTGAGAGTGTG1000.11847920097626861No Hit
AAATTTGGGGTGCATTGTCTGTGACATGCAACTTACAAAACTAGCAATTGTA990.11729440896650592No Hit
GCTCCGGCTGCATGGCTTCACGGGCCACAAGGACTGGATGGACAAGTGTGTG990.11729440896650592No Hit
TTAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA990.11729440896650592No Hit
GCCATGGGTCTGCAGATTTTTTTATTATGGTGCAGCTTTTTTTTAATTATGT990.11729440896650592No Hit
TGAGGCGGGATGTAGTTAACAGATAAAAGTTTAGATAATGAATAAAATCTCA990.11729440896650592No Hit
TCGTTGGGGCAATGAAGCAGAGTTATAGGGATAGATTTATAGCTGGCAGAGT990.11729440896650592No Hit
CACTGTGGGTCTATAATAAAATGTGATAGAACAGTTTCTTCTGATATATGAC990.11729440896650592No Hit
GCATAGGGAAGAGAATTCATTTTCAGCAGACCATGTGACTACTGCAGTTGAG990.11729440896650592No Hit
CATCCAGGGATTTATGGAGGGGCAAGATTTCACCATTCTGTGAAGAACTCTG980.11610961695674325No Hit
CAACCAGGGATGGGTGTGGTTGCTGGGATCTTAGTACAAAACGTGTTAAAGT980.11610961695674325No Hit
GGCTCTGGGCAGGCAGATAGAAGGAGAGCAGAAAGCTCTCCTCAGTTGCTTT980.11610961695674325No Hit
ATTAGCGGGAGTGAATTGCCATTGCATTTATATATTCATTTTGTAACTGTCT970.11492482494698056No Hit
TGAAACAGGGGCTTCCGGTTCTAGGCGCTTCGGGAGCCGCGGCTTATGGTGC970.11492482494698056No Hit
TATACGCGGGCACGTATTAATCACTTCAGCCCTTGCCGACCACCCAGTTCTC970.11492482494698056No Hit
TATCATGGGCTGAACATGAAGCTCTTGATTCCAAAGAGGTAAATGTAAGATA970.11492482494698056No Hit
ATACGACAGGAGATCATCCAGGACTTGGCCTCCATGGTCCGGGAACTTCTTA960.11374003293721786No Hit
CATATTGGGAAGGAGAACTACAAACCACTGCTCTACGAAACTGGTGCCAAGA960.11374003293721786No Hit
TCGCCATGGGTTAACCCAAAGAGGGAGTCCTGGGAACCCCAATTTGAAGACA960.11374003293721786No Hit
ATTTTGGGGGTATGCTGAAACTTTTTTATGGCACAGAAAATGACCTTTCTTA960.11374003293721786No Hit
AGATTAGGGCACCGATAAAAGACAAACAGCAAAATCATCATGAGTGAACGCC950.1125552409274552No Hit
GTCAAAGGGGCTCTTTCATATTTGTGGAACCCTATGTCAAGAGAGAAAACAT950.1125552409274552No Hit
AAACGAGGGGTAGAAATTTAAAAATAACAAATACTATAAACTGTCACACAAA950.1125552409274552No Hit
TTATTTGGGAGGCGGAGGGGAACCAGCAGCTTTGGACCTTATTGATTGTCTG940.1113704489176925No Hit
GGGCACGGGCGGCAGGGCAGTGTGCCCTAATTCCACGAAGAGGGGGCATGGG940.1113704489176925No Hit
GTAGCAGGGTACACTGTTGGAAGGTAATTTCATTGCTATGTTATTAAAATGA940.1113704489176925No Hit
TGCGTTGGGCATTGGTAAATTCTACCAAACATTTAAAGAAGAATTAACACCA940.1113704489176925No Hit
ACATAGGGGGCTTCCTTTAAATGAAAAGGTGAAAGTTCTCAACTTAAAAAAG930.11018565690792981No Hit
CGCATCGGGGAGAGATTTAATGGAAAAATGAGAATTGTTGTGTACATGATTT930.11018565690792981No Hit
ACGCTGGGATTACAGGCGTGAGCCACCACACCCAGCCTGAATACATTTTAGA930.11018565690792981No Hit
CGTACAGGGAGTCCTGCTCCAGCTGTAACAGCAGCATCTGTGACCAACATAC920.10900086489816713No Hit
TAATTAGGGGGCGTTCTGAGCCGTCTGTCCTGCGCCAAGATGCTTCAAAGTA920.10900086489816713No Hit
GACGCTCGGGTCTTTGGGCGGGGTTCATTACAGCACTGCTGTTCATTTCATC920.10900086489816713No Hit
CGAACGGGGGGACTTTTATGGCTCTATCTTTTCCTTCAGTGAAGAACCCTAT920.10900086489816713No Hit
TACTACGGGCTTATTTATCTTAATATTCCCTATATCCCCTAATATCTTATGA910.10781607288840443No Hit
TCATGCGGGAAAAACAGCACGTCTTAAAACTTTTACTGCCAACCAGATAGAT910.10781607288840443No Hit
GCCTTTGGGATGACTTTGCTGCATATATACTCGGTTTTGTTCTTCTATCAGT910.10781607288840443No Hit
CAGCGGGTTCTGGGGTAAAACATAAACCTAGCTGAGTTGCCTTTATCTAAGG910.10781607288840443No Hit
TAATAACGGGGATGAGAGAAAGCAAGAACCCTACTCACTCATGGAGATCTTC910.10781607288840443No Hit
TCGGATGGGGGTGGAAGGGAGGTGGGGATAGTTAATTCGTACAAAAAGAAAA910.10781607288840443No Hit
GTAAGTGGGATGATGTGTAGATAGAGCATGAATGAAATTATTTATTGTATCA900.10663128087864177No Hit
CAATTAGGGACGTGGAGCTGTGATTTCTTAGTGAAGGATTTCCCACAAATAC900.10663128087864177No Hit
TAGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA900.10663128087864177No Hit
CAGCAGGGTATGATTCTTGAGGTAACTCTACCAGATACTTCCAGATTTAGAA900.10663128087864177No Hit
GTACAGGGGGAAACGAGAAAGTACTGAAGGGTAAGAAGCATAGGTAGATTAT900.10663128087864177No Hit
CAAGCCGGGTAACCTATACTGCAGAGGTAAATATTACGAATTATTAACTCAA900.10663128087864177No Hit
GTAGACGGGACGATAGATCAAATGTACCTAACACATATTTACAGAACATTCC900.10663128087864177No Hit
CGAGTTGGGAACCTGTAAACATCTGAATGCTTTCAGGACAAGGCAGGTGTTC900.10663128087864177No Hit
TGTGTGGGGGTCAATATCCAGAGGCGGATAATCAATCAATTGCATTTCACTG900.10663128087864177No Hit
TTATACGGGACATTCAGTTTCTTCTTTCTACCTTTTGCCTAGAATGTTGTCT890.10544648886887907No Hit
GGACCAGGGATACTTATTGTAATGGTATATTCAGATGGCACGATATTTTTGC890.10544648886887907No Hit
AGGTAGGGGGGAGTTCTTTGGCTATTGCACTTTTGAAATTATTTGCAAATAA890.10544648886887907No Hit
CGTACAGGGATTTCTTGAAATTATATTAGTGGTGGGTTAGGAAGTGGATTTA890.10544648886887907No Hit
GGATCAGGGATCCAATGGGAGAGTAGTGGCTCAAGGCTCCTGGGCTGCTGCA880.10426169685911638No Hit
TTTACCGGGATAGATGATGCTTATAGACAAGGACATTTTACCTATAAACCTG880.10426169685911638No Hit
TTTAAAGGGGGGTCTAACGGACCCTAATGAGATACTGAGGATTAAACACTAG880.10426169685911638No Hit
CAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.10426169685911638No Hit
TCAGAAGGGGCATGATTGGATATGAAAATTAAACAGCATAATATTTTAGAGA880.10426169685911638No Hit
TAATATGGGATACAAGGCTGTTTTAGTATTCCAAAATTAATTCATGAAATTT880.10426169685911638No Hit
TAAGGAGGGATCATGTATAAAGCAAACACCATCAGTCTAATAGCAGACCATT880.10426169685911638No Hit
TATCGGGGGCGCTGAGGGGGTCCGGCCGTTTGGCAGCCCGCGAGGCGGTCCG880.10426169685911638No Hit
TTTATAGGGAGATTGAATCTCTTCTGAAATCACGATTTCTTCCTGGTAAATT880.10426169685911638No Hit
AACCTTGGGATTCCTAGTTGCCTTGTCACTGATTTTATACATTTTAAATTGT880.10426169685911638No Hit
CTTTATGGGAGGCCAAAAAAATGAGAGAGAACATTTCATCTTTGATGAGAAA880.10426169685911638No Hit
ACTTACGGGATTTCTATACAGAAACTTGTACATGAATGTTCATGGCAGCTTT870.10307690484935371No Hit
AGAATAGGGAGCTTTTGCATATGCATGTACCTGTGTTACTACCACCCAGATC870.10307690484935371No Hit
CTTCTCGGGCTAGTTAAGCTAAAAAGTAAAAACTTTTTTAACTAGTGGCTTT870.10307690484935371No Hit
TCTAGTGGGGAGATGTGTCTGGTTGTCCGGTTCTAAACCTTTCTACAGGTCA870.10307690484935371No Hit
TTGCTGGGGTGAATAGTTGAAATGTATATTTGTACAGTCTTTTAGACCTATT870.10307690484935371No Hit
CTACGCGGGAAGAGAAATTATTGACCACACTTACTAAAATTCATGCAAAAGC870.10307690484935371No Hit
GCTTCCGGGGAAAACTGGAGTTCATTTTCATAGCAACACAGAAAATAATTCC870.10307690484935371No Hit
AAATGGGGGAAGTGGGTAGATATTTGAATTTCTGAATGCTTTAGAATATTAG870.10307690484935371No Hit
CATGGTGGGAGGGGGATTTCAGCCAAATCCAGATGTCTCTCTGAATTTTCTC870.10307690484935371No Hit
ATTAGCGGGCAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT860.10189211283959101No Hit
TAGCTAGGGCGGGAAGTTAAAATTTTCAGAAACAACAATATTAGTGATTTTT860.10189211283959101No Hit
TTGAATGGGTCTGAGCACGGGACTGGGAATAGTGTTAAACAAAACAGATTCT860.10189211283959101No Hit
GAAAAAGGGATCTGTGCTTCACTCAGTCTAGAAAACCTGCTTGTCATAGCCA860.10189211283959101No Hit
CCAATTGGGGTGGAAAATTAATTACCTTTATGAAGGTGGTTTATTACAAATA860.10189211283959101No Hit
GATGAGGGCAGAGCAGAACTGAATGAAATTCACACAAAGGATCAACAAAACC860.10189211283959101No Hit
TAAACTGGGATAGAGAATGATTAAATGTGAGGATAGCTATTTTAGAAAGGGT860.10189211283959101No Hit
GTTACAGGGAGCATTTCACTCTGCGTTCTCCTAGATCTGAGCAAGCTGTCAG860.10189211283959101No Hit
TAAAACGGGTTGTAAAATGGACTTTCTCATATTTCAATGGCTCTCTCTCTGC860.10189211283959101No Hit
TGTAATGGGGGCTGTGCGCGTAGTCTGAAAAGCAGAAGTCAACATTTTTACA860.10189211283959101No Hit
TACTTCGGGGGAGAGCGCGGAGGGAGGCGCGCGCGGGAGCGAACACCCTCCC850.10070732082982832No Hit
CTTACAGGGACATACAGGTCAGCATGAGAGACTACCTCGGGAACATAATGGA850.10070732082982832No Hit
TATCTCGGGGGAAGCACAGGGGTCCAGTAGAGTCTGAGCTTCCTTTCCTAAG850.10070732082982832No Hit
TAGGCAGGGACCTGCACGCAACCACTTACTTATAAAGCTGTGATAGGGCATG850.10070732082982832No Hit
TTACTAGGGGGGTGAAGGGCGGCGCGCTTAGGCAGGCGGTGGCGCGGCTGGA850.10070732082982832No Hit
CAAACAGGGGGGACCAGAGAATTATCTTTTTATTTCACCTGATTTTGTTTCT850.10070732082982832No Hit
AGTTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC850.10070732082982832No Hit
AACACAGGGAGTGGAACCAATTGTGCCCTTTTCTGTTTGCACGTTTAAGATA850.10070732082982832No Hit
TAGCTGGGATATTCAAGGGCCTTCCAAGTTGTAGAAGAACAATGATCTTCCC850.10070732082982832No Hit
ATACTAGGGATAATGTGAGGGTTAAAAACTCCGGTAGCCAAGACTCTGAGGC850.10070732082982832No Hit
GGTTTTGGGACCATGTTGGCAAACTTAAGAGTAGATGACCTCTCAGTCCCTT850.10070732082982832No Hit
AGTAAAGGGAAGTGTAATAAGAGCTGTAAGTTAATTTTCAAATGAATGTTCA850.10070732082982832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)