FastQCFastQC Report
Sat 31 Dec 2022
SRR5843861.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843861.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84876
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGC4200.49483953060935953No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTATTCAATCTCGTATGCCGTCT3960.46656298600311047No Hit
TGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1350.15905556341015128No Hit
TGCCTAGGGGTGAAATCAGTGCATTGATTAGGCAGAAGAGACATGAACTGGA1330.15669918469296387No Hit
AATTGCGGGGCCAGCTTGTGAAGATGTGAAGGCCAAACCAGTATCCGACAAT1320.15552099533437014No Hit
ACAACTGGGTACCCGAGACTGTGTAATTTATGACGAAAAGAGGTTTAATTCC1300.15316461661718273No Hit
TAGCTAGGGCGGGAAGTTAAAATTTTCAGAAACAACAATATTAGTGATTTTT1240.14609548046562043No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1240.14609548046562043No Hit
TGAGGCGGGATGTAGTTAACAGATAAAAGTTTAGATAATGAATAAAATCTCA1180.13902634431405816No Hit
CAAGACAGGATTGGTGATTTTCTGGTATTTTACCACACTTCTGAGTTGATTT1170.13784815495546443No Hit
CTTTATGGGAGGCCAAAAAAATGAGAGAGAACATTTCATCTTTGATGAGAAA1170.13784815495546443No Hit
TTGATAGGGATCGGAAAATTCATACTGGAGAAAATCTTTACAAATGCAAAGT1120.13195720816249587No Hit
ATCAAGGGAAATCAGAGTCTTTAATTCTTGCATGTTTGTATCTAATTTCTGA1110.13077901880390216No Hit
GGACCAGGGATACTTATTGTAATGGTATATTCAGATGGCACGATATTTTTGC1110.13077901880390216No Hit
GAACATGAGGGGCGGTTGGTCAGACCGGAGCACTTGGCCTGAAGACCTGGAA1100.12960082944530846No Hit
TAGCTAGGGAGGACTGGAAGGTGGCTAGGGATGTGTAGCGGGAGAAGGGTCA1080.12724445072812102No Hit
GCGACCGGGGCATGAAGTTTCGTTGAAATGACAAATACTAATTTCTTCATCT1070.1260662613695273No Hit
TACTGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1070.1260662613695273No Hit
CTACAAGGGCGGTAGCGCTTGCAGCATGGCTGACCAACTGACTGAAGAGCAG1070.1260662613695273No Hit
TTAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1060.1248880720109336No Hit
TTAGCAGGGCAAGAGAAGAGAAATTATTTCAGATGTGCCAGAATAAAACAGA1060.1248880720109336No Hit
ATACGACAGGAGATCATCCAGGACTTGGCCTCCATGGTCCGGGAACTTCTTA1050.12370988265233988No Hit
TATACCTGGGTGTTGCAGTGAGCTGAGATTGCATCACTGCATTCCAGCCTGG1050.12370988265233988No Hit
GACAAGGGCATGGAGCTCAAAACTAATGGGCCCCGGAAGATTGGCTACTGGA1050.12370988265233988No Hit
GACGCTCGGGTCTTTGGGCGGGGTTCATTACAGCACTGCTGTTCATTTCATC1040.12253169329374616No Hit
GTGCAGGGGGAGCTCAGCCAGCCCTGCAGGGCGAGCAGTCCAGCCTTGTGTT1040.12253169329374616No Hit
ATCTATGGGAGTTTCCAGCAGTTAGAACATTTATCATATGTGTCTTTGTAGT1030.12135350393515246No Hit
AGATTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1030.12135350393515246No Hit
CGAGTTGGGAACCTGTAAACATCTGAATGCTTTCAGGACAAGGCAGGTGTTC1020.12017531457655874No Hit
AGCCATCGGGGGAAGCAGGTTAAATGTTTTGTAAACTTTGAAATATATGGTC1020.12017531457655874No Hit
TACCTGGGAAGATCTATTTCATAATCAAAGTTCACATAGAATAAACAAGTGT1010.11899712521796504No Hit
CGTACAGGGAGTCCTGCTCCAGCTGTAACAGCAGCATCTGTGACCAACATAC1010.11899712521796504No Hit
AAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.11899712521796504No Hit
ATTAGCGGGAGTGAATTGCCATTGCATTTATATATTCATTTTGTAACTGTCT1000.11781893585937131No Hit
GCCATGGGTCTGCAGATTTTTTTATTATGGTGCAGCTTTTTTTTAATTATGT1000.11781893585937131No Hit
CTAGCGGGTGCAGACAGATGTGGATTTGGATCCTGGCTCTGCAATGTAGTTA1000.11781893585937131No Hit
GAAAACAGGGTGGTGGAATTTTTATTGAAAAAGAAAGCAAGTGCACAATGCC1000.11781893585937131No Hit
TAACGCGGGGTTTGCATGCTCTGTAAAGTGACTGGTGCACTGTGTAAACTCA1000.11781893585937131No Hit
CAAAGCGGGATGCCCGTGTCAACCACAACAAGTACATGGTGACTGAACGCGC990.11664074650077762No Hit
GCTCCGGCTGCATGGCTTCACGGGCCACAAGGACTGGATGGACAAGTGTGTG990.11664074650077762No Hit
TGAAACAGGGGCTTCCGGTTCTAGGCGCTTCGGGAGCCGCGGCTTATGGTGC990.11664074650077762No Hit
ATACTAGGGATAATGTGAGGGTTAAAAACTCCGGTAGCCAAGACTCTGAGGC990.11664074650077762No Hit
CACTGTGGGTCTATAATAAAATGTGATAGAACAGTTTCTTCTGATATATGAC990.11664074650077762No Hit
TCTGCGGGGGTTTTCACTTTAGTGGTTCTGCCTTCTAGTCCACAGAAAAGTT980.1154625571421839No Hit
ACATAGGGGGCTTCCTTTAAATGAAAAGGTGAAAGTTCTCAACTTAAAAAAG980.1154625571421839No Hit
TCAGAAGGGGCATGATTGGATATGAAAATTAAACAGCATAATATTTTAGAGA980.1154625571421839No Hit
CGCATCGGGGAGAGATTTAATGGAAAAATGAGAATTGTTGTGTACATGATTT980.1154625571421839No Hit
AACACAGGGAGTGGAACCAATTGTGCCCTTTTCTGTTTGCACGTTTAAGATA980.1154625571421839No Hit
GTCCAAGGGATGCAAAGTTATCAAAGAAGAGGAGAAGGAAAGGAGGACAGAT980.1154625571421839No Hit
TTGCTTGGGAAGTGGGAAATGTCTGGCAATAAACTGGTTATTAATGAGGATG980.1154625571421839No Hit
GTACTAGGGAGGTGTGCTCCTGTGAGGCAGGAAGCTGTAGTGGGGAGGCTGT980.1154625571421839No Hit
TTGCTTGGGACGCCTGGACTTTCTGCAGGCCTGGATCCAAGATGGCATCCCA970.11428436778359018No Hit
ATTAGCGGGCAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT970.11428436778359018No Hit
GTTATTGGGAATTGGGGGTTTGGTGAAGAAATCTGATTGTTGTGTGTATTCA970.11428436778359018No Hit
TCGTTGGGGCAATGAAGCAGAGTTATAGGGATAGATTTATAGCTGGCAGAGT970.11428436778359018No Hit
TTGCTAGGGATAAGTGAGTGATGTTATTGTGAGCTTCTAGTGATTCTTAGAG970.11428436778359018No Hit
GTTAATGGGAGAAATGTGAATGTCCAACAGAGTCGTTATAAAGGAATTTTAC970.11428436778359018No Hit
GCTTTAGGGACAAGAGTTTTTATGGGAAATCATTAGGTATTCACTTTACAGA960.11310617842499647No Hit
TAGATCTGGGATTGACATACAAATTCAGGTTTGAATCCTTGCAAAATCACAT960.11310617842499647No Hit
GTCAAAGGGGCTCTTTCATATTTGTGGAACCCTATGTCAAGAGAGAAAACAT960.11310617842499647No Hit
GAAACAGGGGAATAGGGAATAACTATGATAAATTGCTTAAAATTTTATTATA960.11310617842499647No Hit
AAGCATGGGGCCTGCAAAAACAGAGGTGGATACGAGGACAGGTTGGAGCTGC950.11192798906640275No Hit
CAATTAGGGACGTGGAGCTGTGATTTCTTAGTGAAGGATTTCCCACAAATAC950.11192798906640275No Hit
TAGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA950.11192798906640275No Hit
CCGCCAGGGGACGCTCTTCCGATCTCTGTAAGGGAGCTTCTTCATGGAGTAA950.11192798906640275No Hit
TCGCCATGGGTTAACCCAAAGAGGGAGTCCTGGGAACCCCAATTTGAAGACA950.11192798906640275No Hit
CCAATCGGGAGAGAAAATAAGCAGTTACTACCCTGATAGGCACCTTCCCAAT950.11192798906640275No Hit
GACTTAGGGGTGGAATCTGATCAGTTACTTTAAAAAATGACTCCTTATTTTT940.11074979970780904No Hit
GCATTGGGAATTTTGTGTGTGTGTCTGTGCCCATAGAGGAGTGCTGGTTGTG940.11074979970780904No Hit
ATGCCGGGCATGGAGGGAAGTTTGGTTCTTTTCAATTAAGTCCGGAATTAAG940.11074979970780904No Hit
CGTACGGGGACTCATGATCTCATTTAACCTTAATTATTTCCCTAAGGCCTTG930.10957161034921532No Hit
AGGCAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC930.10957161034921532No Hit
TATCGGGGGCGCTGAGGGGGTCCGGCCGTTTGGCAGCCCGCGAGGCGGTCCG930.10957161034921532No Hit
GTCTATGGGACAGGGAAGGTTCTCTCCTTTTCCTTGGTGACAGTGAGAAGTC930.10957161034921532No Hit
AGGTAGGGGGGAGTTCTTTGGCTATTGCACTTTTGAAATTATTTGCAAATAA930.10957161034921532No Hit
GGAAACGGGATGGTATTTACAGTGAAAGAGTAAATTAGAAAGTGAATGGATG930.10957161034921532No Hit
GTACTCGGGGTGGATGATTTGGTGGGAGTAGGTATATACTTAGTGGAATGGT920.1083934209906216No Hit
AAGGTAGGGTAGAGTGGGTGTAGTGAGTGTATATAATGTGATTTGGCCCTGT920.1083934209906216No Hit
TTACCAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGG920.1083934209906216No Hit
TGCATAGGGACTGTCACTTTTGGACTGAAAAGCTGAGGAAGAAACTTAATTT920.1083934209906216No Hit
ATTTTGGGGGTATGCTGAAACTTTTTTATGGCACAGAAAATGACCTTTCTTA920.1083934209906216No Hit
GAAAAAGGGATCTGTGCTTCACTCAGTCTAGAAAACCTGCTTGTCATAGCCA910.10721523163202791No Hit
CAGCAGGGTATGATTCTTGAGGTAACTCTACCAGATACTTCCAGATTTAGAA910.10721523163202791No Hit
GTACAGGGGGAAACGAGAAAGTACTGAAGGGTAAGAAGCATAGGTAGATTAT910.10721523163202791No Hit
AAAAAGGGGGGCTCTCTGAGTTGAGCATGCATCAGTTACCTTAAGGGCTTGT910.10721523163202791No Hit
GCACAAGGGACTATTAAGAATATTATAAAATGTTGTGCCATTTTGCTGTCTT910.10721523163202791No Hit
CACGCAGGGCCTAACATATTTGGAAGTTCACAAATGGGTGCTACCATAGTAG910.10721523163202791No Hit
TTAGCAGGGATGTAACAATCATGAATATTAGATTTTTTAAATTGTAGAATAT910.10721523163202791No Hit
GGATCAGGGATCCAATGGGAGAGTAGTGGCTCAAGGCTCCTGGGCTGCTGCA900.10603704227343418No Hit
CATATTGGGAAGGAGAACTACAAACCACTGCTCTACGAAACTGGTGCCAAGA900.10603704227343418No Hit
GTGTTAGGATTGAAGTAGACAAGGACTTTGAAATAACTGTTTGAAATATTGT900.10603704227343418No Hit
TTGCTGGGGTGAATAGTTGAAATGTATATTTGTACAGTCTTTTAGACCTATT900.10603704227343418No Hit
GATGAGGGCAGAGCAGAACTGAATGAAATTCACACAAAGGATCAACAAAACC900.10603704227343418No Hit
TAATAACGGGGATGAGAGAAAGCAAGAACCCTACTCACTCATGGAGATCTTC900.10603704227343418No Hit
TCCAACGGGAATTTCGGATATTCTTCTTTGATACTACACCAAAACTTGACAA900.10603704227343418No Hit
GGCATAGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTG890.10485885291484048No Hit
TCAGCGGGGTATTATCTTTCTTTATTTGCTAGATTCTTACAAATCTTTTAAG890.10485885291484048No Hit
AAGTGAGGGGGGGGCCTGAATATTCCAGAGCTGATGTGATGGGCTGTGCAGA890.10485885291484048No Hit
TATCATGGGCTGAACATGAAGCTCTTGATTCCAAAGAGGTAAATGTAAGATA890.10485885291484048No Hit
AACCTTGGGATTCCTAGTTGCCTTGTCACTGATTTTATACATTTTAAATTGT890.10485885291484048No Hit
TAAAACGGGTTGTAAAATGGACTTTCTCATATTTCAATGGCTCTCTCTCTGC890.10485885291484048No Hit
GCATAGGGAAGAGAATTCATTTTCAGCAGACCATGTGACTACTGCAGTTGAG890.10485885291484048No Hit
GCTTTGGGGTATGAGGTATAATTCATTGTGTTTTATTTTACAGACAAGTGAT890.10485885291484048No Hit
TGTGTGGGGGTCAATATCCAGAGGCGGATAATCAATCAATTGCATTTCACTG890.10485885291484048No Hit
TATTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC880.10368066355624676No Hit
TAGGCAGGGACCTGCACGCAACCACTTACTTATAAAGCTGTGATAGGGCATG880.10368066355624676No Hit
CGCTGGGGAGCTACTAAAAGCATTCATGTACAATTTTTTGTTGGGACACATG880.10368066355624676No Hit
CGCGCAAGGGAGGGTTGTAATGGACTTATGCTGATGCATCAGATGGGGCATT880.10368066355624676No Hit
ACTTGCGGGATTATGGGAAAGCGATCATCTAATGAAGTGTTGAATGTGATAC880.10368066355624676No Hit
TCATGCGGGGATAAATTTCTATGTTGTTTAACTATTTTACAGTGAAAACATA870.10250247419765304No Hit
AGTGACGGGGGATCATCAGCATGATTTCAAAGGGAAACTCTTAGACAAACGA870.10250247419765304No Hit
AACGCCGGGTATCTATATAGAACAAGATATTAAACATTGGTACAATAATGTT870.10250247419765304No Hit
ACCAAGGGATGAAAGCTGTAGACCCTGTCCATAGAAAAAATAGTGCACACTC870.10250247419765304No Hit
CACGACGGGATTGAAGGTGGAGCTCATGAAGGACTTCAGCATTTGGGTCCTT870.10250247419765304No Hit
TGCGTTGGGCATTGGTAAATTCTACCAAACATTTAAAGAAGAATTAACACCA870.10250247419765304No Hit
TAATATGGGATACAAGGCTGTTTTAGTATTCCAAAATTAATTCATGAAATTT870.10250247419765304No Hit
GGCTCTGGGCAGGCAGATAGAAGGAGAGCAGAAAGCTCTCCTCAGTTGCTTT870.10250247419765304No Hit
CAGAAAGGGGTTTTAAAGTAAATGATTATTATAAATTCCAATGGAACAGATA870.10250247419765304No Hit
GTTCATGGGACTGAGAGAGGGATGTTGAAGTCCTCAACTCTTATTTTGGACT870.10250247419765304No Hit
TGTAATGGGGGCTGTGCGCGTAGTCTGAAAAGCAGAAGTCAACATTTTTACA870.10250247419765304No Hit
ATCTCAGGGTATTCCGAGAATTTCTCCTGCCCAGGCATCCTCCTATTTTTCA870.10250247419765304No Hit
CTTGTTGGGAAATCCAGTATTAGCTGCCTATTTCAGACACTTAATACTTGCA860.10132428483905934No Hit
ATATAGTGGGGGTCTTCGCCCGCCATGGCCGAGAGCGACTGGGACACGGTGA860.10132428483905934No Hit
TGACCGGCGGTCAGTCTGCGGCAGCGGCAGCAAGACGGTGCAGTGAAGGAGA860.10132428483905934No Hit
ATTCAAGGGGGACATTGCTCCATCATTTACTAGGTAAGAGACTGAGAGTGTG860.10132428483905934No Hit
CAAATTGGGGATAATCTCAGTTAAAGCATACAATTAAGATTTAAGTTTTCAC860.10132428483905934No Hit
TTGACTGGGATTCCCAGATTCTTTCCTGAATAGATTTGAATTTAATGTCATT860.10132428483905934No Hit
TAATACGGTAAGTTCACATTTACTGTTTGTGTTAAGTGAATCCCCAACCTAC860.10132428483905934No Hit
GTTTAAGGGAACTTCTTTAGGTAAGATCTTATTTACTTTTCCTTTCTTAATT860.10132428483905934No Hit
GCGCCTGGGGGAATGGGAAAGGAGACAATGGCAACAAAATGTTGGAAGCTGG860.10132428483905934No Hit
GGTACAGGGAAGTAAAAGGACCATAGAGGGGAACCAGGAATCTGAGAATCAG860.10132428483905934No Hit
CGTACAGGGATTTCTTGAAATTATATTAGTGGTGGGTTAGGAAGTGGATTTA860.10132428483905934No Hit
TACTGGGGGCAACCCCCTGACAAAACACATAGCCTACCCCTTCCTTGTACTA850.10014609548046562No Hit
TTTACCGGGATAGATGATGCTTATAGACAAGGACATTTTACCTATAAACCTG850.10014609548046562No Hit
CAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA850.10014609548046562No Hit
TTACTAGGGGGGTGAAGGGCGGCGCGCTTAGGCAGGCGGTGGCGCGGCTGGA850.10014609548046562No Hit
CAACCAGGGATGGGTGTGGTTGCTGGGATCTTAGTACAAAACGTGTTAAAGT850.10014609548046562No Hit
AAACGAGGGGTAGAAATTTAAAAATAACAAATACTATAAACTGTCACACAAA850.10014609548046562No Hit
TATACGCGGGCACGTATTAATCACTTCAGCCCTTGCCGACCACCCAGTTCTC850.10014609548046562No Hit
CAAGCCGGGTAACCTATACTGCAGAGGTAAATATTACGAATTATTAACTCAA850.10014609548046562No Hit
CGACTGGGCGCTCTTCCGATCTGTATAGGGGGAGGCATTGAGGCAGCCAGCG850.10014609548046562No Hit
ACGCTGGGATTACAGGCGTGAGCCACCACACCCAGCCTGAATACATTTTAGA850.10014609548046562No Hit
GATCTTGGGGAAAATAACTCTGATGGGATAAAGCCCCCTTTGTAAATTTAGA850.10014609548046562No Hit
GGTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA850.10014609548046562No Hit
GTAGACGGGACGATAGATCAAATGTACCTAACACATATTTACAGAACATTCC850.10014609548046562No Hit
TTTGGTGGGCCTTGAGGAAGGCAAATGTCTGTAGGCTTTGCATATCTCATTT850.10014609548046562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)