FastQCFastQC Report
Sat 31 Dec 2022
SRR5843806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124959
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTAGACGATCTCGTATGC9450.7562480493601902No Hit
CTTATACACATCTCCGAGCCCACGAGACATTAGACGATCTCGTATGCCGTCT7600.6081994894325339RNA PCR Primer, Index 33 (95% over 21bp)
TTAAATGGGATTAGGCATGTTAGCAGACTTCAACAACCTTTTATAGGATTGA1340.10723517313678886No Hit
ACATTTGGGATATGTAGCATGGATTTGTGCTATATTGGAAGTAAAACACTAT1340.10723517313678886No Hit
TACGCCGGGAGGTGATTTTGGAAATCCTGTAAGTTACACTTCCTGTTCTGGT1340.10723517313678886No Hit
TTGGTTGGGTCCCCAATTCCTAGTATAAGGCATTTATTCTCAGAGGTTCAGC1300.10403412319240711No Hit
ATGTAGGGGTACAGTAAGGCAAGCACAGGGACAAAGTGATGAAGGGTTAAAA1300.10403412319240711No Hit
GCCTAAGGGCGGGGGGAAAGATGATGGAGGGGAACAGAAACTGGACTTGATG1250.10003281076192991No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)