FastQCFastQC Report
Sat 31 Dec 2022
SRR5843805.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5843805.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124629
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACATTAGACGATCTCGTATGC9020.7237480843142446No Hit
CTTATACACATCTCCGAGCCCACGAGACATTAGACGATCTCGTATGCCGTCT7420.595367049402627RNA PCR Primer, Index 33 (95% over 21bp)
TTCCTAGGGCAGTTTTCTTATTTTATTGACTACCCCTGATATTTTCCTTTTT1390.11153102407946787No Hit
TCAATAGGGAGCCCTACTGCATCATCTGCGTCAGCCGGTCCTAGCCCATCTG1390.11153102407946787No Hit
GCTGCCAACAAAGTCATTCAAAACAAGAGAACAGAAAATGTGCCAGGGCCAA1370.10992626114307263No Hit
GCCTATGGGAGGCTGGAGTGCAGTGGCACGATTTTGGCTCACTGCAACCTCT1310.10511197233388699No Hit
TTGGTTGGGTCCCCAATTCCTAGTATAAGGCATTTATTCTCAGAGGTTCAGC1300.10430959086568936No Hit
ATAACTGGGATTTTTGTACATCTGTACAATGTGTTTGTATTTTAAGTGTTAC1280.10270482792929414No Hit
GACCAGGAGGTGTTTTGCAAATACGTGAAGCAAAAGACTCCATCAGTGTGAC1280.10270482792929414No Hit
ATCCTGGGGTAGGAAGAATCAATATCGTGAAAATGGCCATACTACCCAAGGT1270.10190244646109653No Hit
TTTCCAGGGAATTTAGCATATTGACAAAGTAATCTGGATTTATACCTTTTAA1270.10190244646109653No Hit
GCCTAAGGGCGGGGGGAAAGATGATGGAGGGGAACAGAAACTGGACTTGATG1250.10029768352470131No Hit
AAACATGGGATCCAGAGATTAAACGAAAACAATCACCTGAAAAGGCTCTGAA1250.10029768352470131No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)